LOCUS       sequence-169711-       11958 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible dCas9 integration vector for Bacillus subtilis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11958)
  AUTHORS   Westbrook AW, Moo-Young M, Chou CP
  TITLE     Development of a CRISPR-Cas9 toolkit for comprehensive engineering 
            of Bacillus subtilis.
  JOURNAL   Appl Environ Microbiol. 2016 Jun 3. pii: AEM.01159-16.
  PUBMED    27260361
REFERENCE   2  (bases 1 to 11958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2016 Jun 3. pii: AEM.01159-16."
COMMENT     Sequence Label: pAW019-2
FEATURES             Location/Qualifiers
     source          1..11958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      243..289
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        complement(324..428)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      1719..1805
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1824..1851
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      1931..1965
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             3595..3786
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(3780..3802)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3888..4028
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3943..3962
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4043..4060)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4214..4802)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4294..4313)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4973..5764,5765..5833))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5596..5615
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5834..5938)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      6582..6616
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             join(8071..8097,8098..8100,8101..10587,10588..10590,
                     10591..11958,1..219)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 catagagaca aaccaatacg tgaacaagca gaaaatatta ttcatttatt tacgttgacg
       61 aatcttggag ctcccgctgc ttttaaatat tttgatacaa caattgatcg taaacgatat
      121 acgtctacaa aagaagtttt agatgccact cttatccatc aatccatcac tggtctttat
      181 gaaacacgca ttgatttgag tcagctagga ggtgactaac tcgagtaagg atctccaggc
      241 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc ctgcaggaga
      301 tccgagctcc cgcgggcggc cgcactcttc ctttttcaat attattgaag catttatcag
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
      481 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attgatcctc
      541 tagcacaaaa agaaaaacga aatgatacac caatcagtgc aaaaaaagat ataatgggag
      601 ataagacggt tcgtgttcgt gctgacttgc accatatcat aaaaatcgaa acagcaaaga
      661 atggcggaaa cgtaaaagaa gttatggaaa taagacttag aagcaaactt aagagtgtgt
      721 tgatagtgca gtatcttaaa attttgtata ataggaattg aagttaaatt agatgctaaa
      781 aatttgtaat taagaaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt
      841 ttaacgagtg aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat
      901 accgtttacg aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt
      961 aaacaggtaa cgtctattga attagacagt catctattca acttatcgtc agaaaaatta
     1021 aaactgaata ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac
     1081 aaacagaggt ataaaattgt tgggagtatt ccttaccatt taagcacaca aattattaaa
     1141 aaagtggttt ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac
     1201 aagcgtacct tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt
     1261 cagcaattgc ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc
     1321 ttaataaaac ttacccgcca taccacagat gttccagata aatattggaa gctatatacg
     1381 tactttgttt caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt
     1441 catcaagcaa tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta
     1501 ttgtctattt ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt
     1561 ttgtaaattt ggaaagttac acgttactaa agggaatgta gataaattat taggtatact
     1621 actgacagct tccaaggagc taaagaggtc cctagactct agacccgggg atctctgcag
     1681 tcgggaagat ctggtaatga ctctctagct tgaggcatca aataaaacga aaggctcagt
     1741 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
     1801 caaatccgcc gctctagcta agcagaaggc catcctgacg gatggccttt ttgcgtttct
     1861 acaaactctt gttaactcta gagctgcctg ccgcgtttcg gtgatgaaga tcttcccgat
     1921 gattaattaa ttcagaacgc tcggttgccg ccgggcgttt tttatgcagc aatggcaaga
     1981 acgttgctct agagcggccg catcgattca cagtggcaat ctcccccgta ttcgtttgaa
     2041 atgtgccaca ttaacagcgc cgggtgatgt ccgtatcgtt ctgctaataa gcggttgatg
     2101 tgccgtgttt tttctcggta gactttagat gtgaggcagt ggttgtgcct tccgccgtgc
     2161 agctgtttga cgcgggaggc attgacgcgc aaaacttccg gataggtttg cgacagccag
     2221 gccggacggg ctccgctcgg cgttgctaat atgacccggc cgcctatact gtgaatccgc
     2281 tcaaaaatat catccagcca ttcaaattga tatacgccct cctccggctc aagtgcgctc
     2341 catgcaaaaa tgccgacaga aaacgtattc gtatgagaaa gcttcatcag tttgatatcg
     2401 tcagctaaaa tatcgggccg atccagccac tgatcggggt tgtagtctcc cccatggagc
     2461 ataaattttg cttttgttac gtgcgttttt tcaagctttg acatcacccc atcctgatat
     2521 tgtctgcatt tgcgccggag caaaccaaaa acctggtgag acacgccttg aattagtaga
     2581 agagaacttg aagattttca aaggcatcgt tagtgaagtc atggcgagcg gatttgacgg
     2641 cattttctta gtcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     2701 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     2761 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcattggacc
     2821 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
     2881 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
     2941 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
     3001 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
     3061 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
     3121 tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
     3181 ggttgcctta ctggttagca gaatgaacca ccgatacgcg agcgaacgtg aagcgactgc
     3241 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
     3301 taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
     3361 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
     3421 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
     3481 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
     3541 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
     3601 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     3661 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     3721 gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     3781 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     3841 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     3901 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     3961 tactgagagt gcaccatatg cggtatgaaa taccgcacag atgcgtaagg agaaaatacc
     4021 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     4081 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4141 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4201 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4261 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4321 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4381 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4441 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4501 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4561 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4621 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     4681 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4741 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4801 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4861 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4921 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4981 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     5041 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     5101 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     5161 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     5221 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     5281 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     5341 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     5401 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     5461 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     5521 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     5581 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     5641 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5701 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5761 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5821 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5881 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5941 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     6001 ataaaaatag gcgtatcacg aggccctttc gtcttcaaga atgatgggct aatgtgtgtt
     6061 tacgacaatt ctcacttcat acttttccat cgtcaggtcg cctgacaata tgtctcctgt
     6121 cattatgtcc ttcacactct gatcaaacgt gaccagctgt ttttcttccg tgaaattcat
     6181 gacaaaaata taatcattgt cctgatcctg cctcgcttgt acggagacgc cttttccgtg
     6241 ccgaaccgga aaaactggag agagagacag gtctgtgatc agaccctcat agaaatcacg
     6301 ctgaaattga tcctccaaac gcgcgccgat aaaatacgcc ttgccctgct gatactcatg
     6361 gcttgtgacc gctggcgtgc gcgcataaaa atcttcttga tacaccgctt ccactgaagc
     6421 tgtctttaca tcaatcacgg ttgcataatc cttcatttca tatatttggc tgcggtagct
     6481 gacagcgttt cgatccttcg gatacagggt gtccgtttca agaggctcaa ctccaaatat
     6541 agcttgaaat cgatatctct gcagtcgcga tgattaatta attcagaacg ctcggttgcc
     6601 gccgggcgtt ttttatgcag caatggcaag aacgtcccgg ggagctccta acttataggg
     6661 gtaacactta aaaaagaatc aataacgata gaaaccgctc ctaaagcagg tgcatttttt
     6721 cctaacgaag aaggcaatag ttcacattta ttgtctaaat gagaatggac tctagaagaa
     6781 acttcgtttt taatcgtatt taaaacaatg ggatgagatt caattatatg atttctcaag
     6841 ataacagctt ctatatcaaa tgtattaagg atattggtta atccaattcc gatataaaag
     6901 ccaaagtttt gaagtgcatt taacatttct acatcatttt tatttgcgcg ttccacaatc
     6961 tcttttcgag aaatattctt ttcttcttta gagagcgaag ccagtaacgc tttttcagaa
     7021 gcatataatt cccaacagcc tcgatttcca cagctgcatt tgggtccatt aaaatctatc
     7081 gtcatatgac ccatttcccc agaaaaaccc tgaacacctt tatacaattc gttgttaata
     7141 acaagtccag ttccaattcc gatattaata ctgatgtaaa cgatgttttc atagtttttt
     7201 gtcataccaa atactttttc accgtatgct cctgcattag cttcattttc aacaaaaacc
     7261 ggaacattaa actcactctc aattaaaaac tgcaaatctt tgatattcca atttaagtta
     7321 ggcatgaaaa taatttgctg atgacgatct acaaggcctg gaacacaaat tcctattccg
     7381 actagaccat aaggggactc aggcatatgg gttacaaaac catgaataag tgcaaataaa
     7441 atctctttta cttcactagc ggaagaacta gacaagtcag aagtcttctc gagaataata
     7501 tttccttcta agtcggttag aattccgtta agatagtcga ctcctatatc aataccaatc
     7561 gagtagcctg cattcttatt aaaaacaagc attacaggtc ttctgccgcc tctagattgc
     7621 cctgccccaa tttcaaaaat aaaatctttt tcaagcagtg tatttacttg agaggagaca
     7681 gtagacttgt ttaatcctgt aatctcagag agagttgccc tggagacagg ggagttcttc
     7741 aaaatttcat ctaatattaa tttttgattc atttttttta ctaaagcttg atctgcaatt
     7801 tgaataataa ccactccttt gtttatccac cgaactaagt tggtgttttt tgaagcttga
     7861 attagatatt taaaagtatc atatctaata ttataactaa attttctaaa aaaaacattg
     7921 aaataaacat ttattttgta tatgatgaga taaagttagt ttattggata aacaaactaa
     7981 ctcaattaag atagttgatg gataaacttg ttcacttaaa tcaaaggggg aaatgacaaa
     8041 tggtccaaac tagtaaagga ggtatcaagt atggataaga aatactcaat aggcttagct
     8101 atcggcacaa atagcgtcgg atgggcggtg atcactgatg aatataaggt tccgtctaaa
     8161 aagttcaagg ttctgggaaa tacagaccgc cacagtatca aaaaaaatct tataggggct
     8221 cttttatttg acagtggaga gacagcggaa gcgactcgtc tcaaacggac agctcgtaga
     8281 aggtatacac gtcggaagaa tcgtatttgt tatctacagg agattttttc aaatgagatg
     8341 gcgaaagtag atgatagttt ctttcatcga cttgaagagt cttttttggt ggaagaagac
     8401 aagaagcatg aacgtcatcc tatttttgga aatatagtag atgaagttgc ttatcatgag
     8461 aaatatccaa ctatctatca tctgcgaaaa aaattggtag attctactga taaagcggat
     8521 ttgcgcttaa tctatttggc cttagcgcat atgattaagt ttcgtggtca ttttttgatt
     8581 gagggagatt taaatcctga taatagtgat gtggacaaac tatttatcca gttggtacaa
     8641 acctacaatc aattatttga agaaaaccct attaacgcaa gtggagtaga tgctaaagcg
     8701 attctttctg cacgattgag taaatcaaga cgattagaaa atctcattgc tcagctcccc
     8761 ggtgagaaga aaaatggctt atttgggaat ctcattgctt tgtcattggg tttgacccct
     8821 aattttaaat caaattttga tttggcagaa gatgctaaat tacagctttc aaaagatact
     8881 tacgatgatg atttagataa tttattggcg caaattggag atcaatatgc tgatttgttt
     8941 ttggcagcta agaatttatc agatgctatt ttactttcag atatcctaag agtaaatact
     9001 gaaataacta aggctcccct atcagcttca atgattaaac gctacgatga acatcatcaa
     9061 gacttgactc ttttaaaagc tttagttcga caacaacttc cagaaaagta taaagaaatc
     9121 ttttttgatc aatcaaaaaa cggatatgca ggttatattg atgggggagc tagccaagaa
     9181 gaattttata aatttatcaa accaatttta gaaaaaatgg atggtactga ggaattattg
     9241 gtgaaactaa atcgtgaaga tttgctgcgc aagcaacgga cctttgacaa cggctctatt
     9301 ccccatcaaa ttcacttggg tgagctgcat gctattttga gaagacaaga agacttttat
     9361 ccatttttaa aagacaatcg tgagaagatt gaaaaaatct tgacttttcg aattccttat
     9421 tatgttggtc cattggcgcg tggcaatagt cgttttgcat ggatgactcg gaagtctgaa
     9481 gaaacaatta ccccatggaa ttttgaagaa gttgtcgata aaggtgcttc agctcaatca
     9541 tttattgaac gcatgacaaa ctttgataaa aatcttccaa atgaaaaagt actaccaaaa
     9601 catagtttgc tttatgagta ttttacggtt tataacgaat tgacaaaggt caaatatgtt
     9661 actgaaggaa tgcgaaaacc agcatttctt tcaggtgaac agaagaaagc cattgttgat
     9721 ttactcttca aaacaaatcg aaaagtaacc gttaagcaat taaaagaaga ttatttcaaa
     9781 aaaatagaat gttttgatag tgttgaaatt tcaggagttg aagatagatt taatgcttca
     9841 ttaggtacct accatgattt gctaaaaatt attaaagata aagatttttt ggataatgaa
     9901 gaaaatgaag atatcttaga ggatattgtt ttaacattga ccttatttga agatagggag
     9961 atgattgagg aaagacttaa aacatatgct cacctctttg atgataaggt gatgaaacag
    10021 cttaaacgtc gccgttatac tggttgggga cgtttgtctc gaaaattgat taatggtatt
    10081 agggataagc aatctggcaa aacaatatta gattttttga aatcagatgg ttttgccaat
    10141 cgcaatttta tgcagctgat ccatgatgat agtttgacat ttaaagaaga cattcaaaaa
    10201 gcacaagtgt ctggacaagg cgatagttta catgaacata ttgcaaattt agctggtagc
    10261 cctgctatta aaaaaggtat tttacagact gtaaaagttg ttgatgaatt ggtcaaagta
    10321 atggggcggc ataagccaga aaatatcgtt attgaaatgg cacgtgaaaa tcagacaact
    10381 caaaagggcc agaaaaattc gcgagagcgt atgaaacgaa tcgaagaagg tatcaaagaa
    10441 ttaggaagtc agattcttaa agagcatcct gttgaaaata ctcaattgca aaatgaaaag
    10501 ctctatctct attatctcca aaatggaaga gacatgtatg tggaccaaga attagatatt
    10561 aatcgtttaa gtgattatga tgtcgatgcc attgttccac aaagtttcct taaagacgat
    10621 tcaatagaca ataaggtctt aacgcgttct gataaaaatc gtggtaaatc ggataacgtt
    10681 ccaagtgaag aagtagtcaa aaagatgaaa aactattgga gacaacttct aaacgccaag
    10741 ttaatcactc aacgtaagtt tgataattta acgaaagctg aacgtggagg tttgagtgaa
    10801 cttgataaag ctggttttat caaacgccaa ttggttgaaa ctcgccaaat cactaagcat
    10861 gtggcacaaa ttttggatag tcgcatgaat actaaatacg atgaaaatga taaacttatt
    10921 cgagaggtta aagtgattac cttaaaatct aaattagttt ctgacttccg aaaagatttc
    10981 caattctata aagtacgtga gattaacaat taccatcatg cccatgatgc gtatctaaat
    11041 gccgtcgttg gaactgcttt gattaagaaa tatccaaaac ttgaatcgga gtttgtctat
    11101 ggtgattata aagtttatga tgttcgtaaa atgattgcta agtctgagca agaaataggc
    11161 aaagcaaccg caaaatattt cttttactct aatatcatga acttcttcaa aacagaaatt
    11221 acacttgcaa atggagagat tcgcaaacgc cctctaatcg aaactaatgg ggaaactgga
    11281 gaaattgtct gggataaagg gcgagatttt gccacagtgc gcaaagtatt gtccatgccc
    11341 caagtcaata ttgtcaagaa aacagaagta cagacaggcg gattctccaa ggagtcaatt
    11401 ttaccaaaaa gaaattcgga caagcttatt gctcgtaaaa aagactggga tccaaaaaaa
    11461 tatggtggtt ttgatagtcc aacggtagct tattcagtcc tagtggttgc taaggtggaa
    11521 aaagggaaat cgaagaagtt aaaatccgtt aaagagttac tagggatcac aattatggaa
    11581 agaagttcct ttgaaaaaaa tccgattgac tttttagaag ctaaaggata taaggaagtt
    11641 aaaaaagact taatcattaa actacctaaa tatagtcttt ttgagttaga aaacggtcgt
    11701 aaacggatgc tggctagtgc cggagaatta caaaaaggaa atgagctggc tctgccaagc
    11761 aaatatgtga attttttata tttagctagt cattatgaaa agttgaaggg tagtccagaa
    11821 gataacgaac aaaaacaatt gtttgtggag cagcataagc attatttaga tgagattatt
    11881 gagcaaatca gtgaattttc taagcgtgtt attttagcag atgccaattt agataaagtt
    11941 cttagtgcat ataacaaa
//
