LOCUS       sequence-169712-       10724 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Golden Gate Level 1 cassette encoding a Cas9-YFP fusion under the 
            FCP promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10724)
  AUTHORS   Hopes A, Nekrasov V, Kamoun S, Mock T
  TITLE     Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira
            pseudonana.
  JOURNAL   Plant Methods. 2016 Nov 24;12:49. doi: 10.1186/s13007-016-0148-0. 
            eCollection 2016.
  PUBMED    27904648
REFERENCE   2  (bases 1 to 10724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2016-11-24"; volume: "12:49. doi"; pages: " 
            10.1186/s13007-016-0148-0. eCollection 2016"
COMMENT     Sequence Label: pICH47742:FCP:Cas9YFP
FEATURES             Location/Qualifiers
     source          1..10724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1014..5117
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1014..5117
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1014..5117
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5130..5150
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5157..5159,5160..5162,5163..5876)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5202..5223)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5463..5482)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5810..5831
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6427..6451
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(6607..7755)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(8091..8679)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8171..8190)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8850..9641,9642..9710))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9473..9492
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9711..9815)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9902..10533
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    10625..10649
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tctgtgaaga caagcaagaa ttcaagcttg gagagcttgc gctttttccg agaactcccc
       61 ataagtcaac ggctccaatc aagaatgtat ccgacaacgg cgagcatagc aacacgtccg
      121 tctttggagt agaatcatca tgttgtggat gaatacacag atgaatgaca ttagaagcat
      181 gaacgtgtta gagagtagga ggtagagatt gatatggtag cattgcgatg tttgtttttg
      241 gtcagcatat gatgagtgga taccaatatg atgaaagttg aatctcgcgt ttgagctcag
      301 cggtacgtta ttgatcgaaa gtagcctgat caaaatcctt ggagagtaca agaggatcaa
      361 agaatccagt gggggcgata actccaagct cgttctcaaa gaggcaatgg aggtagaaac
      421 tcatcccagt tgagaagaag tgaaggcagt ggcggtggcg aaagcagagg caacgaggtc
      481 agacttcctg taggttgatg caacgaatat ttccagaagg agaagtttag agagttgaac
      541 cgctacctac aatgacaaag tatcgtatcg attttgatgt tggttggtta tgaattcaaa
      601 ctgtaagttg gattgtgaga agatcagaag ttgaacgaac acatctttcc gatcattcac
      661 ctccacactg caacaacacg gtacttcttc cgcggcagat ctctgtcgcc attctcttgt
      721 cctgttgttg gctgtgagac gaggaaagca acgacaagtt tcacaaaagg gagttccttt
      781 aacgagatat gttttttata aagagtccca atagaaagac aaattgattc ctccgtgcaa
      841 acgcgcaaat aaacaccacg tccattatat ccatatcttt cagagtatcc aacaagtgtt
      901 gaaggacagg tagttgaagt aacgtatctt ccccctcgac tggatccatc aacaaggcga
      961 acaaatccat tcaacctctc ataaattatc tgatttacca aaccaatacc aaaatggaca
     1021 agaagtactc cattgggctc gatatcggca caaacagcgt cggctgggcc gtcattacgg
     1081 acgagtacaa ggtgccgagc aaaaaattca aagttctggg caataccgat cgccacagca
     1141 taaagaagaa cctcattggc gccctcctgt tcgactccgg ggagacggcc gaagccacgc
     1201 ggctcaaaag aacagcacgg cgcagatata cccgcagaaa gaatcggatc tgctacctgc
     1261 aggagatctt tagtaatgag atggctaagg tggatgactc tttcttccat aggctggagg
     1321 agtccttttt ggtggaggag gataaaaagc acgagcgcca cccaatcttt ggcaatatcg
     1381 tggacgaggt ggcgtaccat gaaaagtacc caaccatata tcatctgagg aagaagcttg
     1441 tagacagtac tgataaggct gacttgcggt tgatctatct cgcgctggcg catatgatca
     1501 aatttcgggg acacttcctc atcgaggggg acctgaaccc agacaacagc gatgtcgaca
     1561 aactctttat ccaactggtt cagacttaca atcagctttt cgaagagaac ccgatcaacg
     1621 catccggagt tgacgccaaa gcaatcctga gcgctaggct gtccaaatcc cggcggctcg
     1681 aaaacctcat cgcacagctc cctggggaga agaagaacgg cctgtttggt aatcttatcg
     1741 ccctgtcact cgggctgacc cccaacttta aatctaactt cgacctggcc gaagatgcca
     1801 agcttcaact gagcaaagac acctacgatg atgatctcga caatctgctg gcccagatcg
     1861 gcgaccagta cgcagacctt tttttggcgg caaagaacct gtcagacgcc attctgctga
     1921 gtgatattct gcgagtgaac acggagatca ccaaagctcc gctgagcgct agtatgatca
     1981 agcgctatga tgagcaccac caagacttga ctttgctgaa ggcccttgtc agacagcaac
     2041 tgcctgagaa gtacaaggaa attttcttcg atcagtctaa aaatggctac gccggataca
     2101 ttgacggcgg agcaagccag gaggaatttt acaaatttat taagcccatc ttggaaaaaa
     2161 tggacggcac cgaggagctg ctggtaaagc ttaacagaga agatctgttg cgcaaacagc
     2221 gcactttcga caatggaagc atcccccacc agattcacct gggcgaactg cacgctatcc
     2281 tcaggcggca agaggatttc tacccctttt tgaaagataa cagggaaaag attgagaaaa
     2341 tcctcacatt tcggataccc tactatgtag gccccctcgc ccggggaaat tccagattcg
     2401 cgtggatgac tcgcaaatca gaagagacta tcactccctg gaacttcgag gaagtcgtgg
     2461 ataagggggc ctctgcccag tccttcatcg aaaggatgac taactttgat aaaaatctgc
     2521 ctaacgaaaa ggtgcttcct aaacactctc tgctgtacga gtacttcaca gtttataacg
     2581 agctcaccaa ggtcaaatac gtcacagaag ggatgagaaa gccagcattc ctgtctggag
     2641 agcagaagaa agctatcgtg gacctcctct tcaagacgaa ccggaaagtt accgtgaaac
     2701 agctcaaaga agattatttc aaaaagattg aatgtttcga ctctgttgaa atcagcggag
     2761 tggaggatcg cttcaacgca tccctgggaa cgtatcacga tctcctgaaa atcattaaag
     2821 acaaggactt cctggacaat gaggagaacg aggacattct tgaggacatt gtcctcaccc
     2881 ttacgttgtt tgaagatagg gagatgattg aagaacgctt gaaaacttac gctcatctct
     2941 tcgacgacaa agtcatgaaa cagctcaaga ggcgccgata tacaggatgg gggcggctgt
     3001 caagaaaact gatcaatggg atccgagaca agcagagtgg aaagacaatc ctggattttc
     3061 ttaagtccga tggatttgcc aaccggaact tcatgcagtt gatccatgat gactctctca
     3121 cctttaagga ggacatccag aaagcacaag tttctggcca gggggacagt ctccacgagc
     3181 acatcgctaa tcttgcaggt agcccagcta tcaaaaaggg aatactgcag accgttaagg
     3241 tcgtggatga actcgtcaaa gtaatgggaa ggcataagcc cgagaatatc gttatcgaga
     3301 tggcccgaga gaaccaaact acccagaagg gacagaagaa cagtagggaa aggatgaaga
     3361 ggattgaaga gggtataaaa gaactggggt cccaaatcct taaggaacac ccagttgaaa
     3421 acacccagct tcagaatgag aagctctacc tgtactacct gcagaacggc agggacatgt
     3481 acgtggatca ggaactggac atcaatcggc tctccgacta cgacgtggat catatcgtgc
     3541 cccagtcttt tctcaaagat gattctattg ataataaagt gttgacaaga tccgataaaa
     3601 atagagggaa gagtgataac gtcccctcag aagaagttgt caagaaaatg aaaaattatt
     3661 ggcggcagct gctgaacgcc aaactgatca cacaacggaa gttcgataat ctgactaagg
     3721 ctgaacgagg tggcctgtct gagttggata aagccggctt catcaaaagg cagcttgttg
     3781 agacacgcca gatcaccaag cacgtggccc aaattctcga ttcacgcatg aacaccaagt
     3841 acgatgaaaa tgacaaactg attcgagagg tgaaagttat tactctgaag tctaagctgg
     3901 tttcagattt cagaaaggac tttcagtttt ataaggtgag agagatcaac aattaccacc
     3961 atgcgcatga tgcctacctg aatgcagtgg taggcactgc acttatcaaa aaatatccca
     4021 agcttgaatc tgaatttgtt tacggagact ataaagtgta cgatgttagg aaaatgatcg
     4081 caaagtctga gcaggaaata ggcaaggcca ccgctaagta cttcttttac agcaatatta
     4141 tgaatttttt caagaccgag attacactgg ccaatggaga gattcggaag cgaccactta
     4201 tcgaaacaaa cggagaaaca ggagaaatcg tgtgggacaa gggtagggat ttcgcgacag
     4261 tccggaaggt cctgtccatg ccgcaggtga acatcgttaa aaagaccgaa gtacagaccg
     4321 gaggcttctc caaggaaagt atcctcccga aaaggaacag cgacaagctg atcgcacgca
     4381 aaaaagattg ggaccccaag aaatacggcg gattcgattc tcctacagtc gcttacagtg
     4441 tactggttgt ggccaaagtg gagaaaggga agtctaaaaa actcaaaagc gtcaaggaac
     4501 tgctgggcat cacaatcatg gagcgatcaa gcttcgaaaa aaaccccatc gactttctcg
     4561 aggcgaaagg atataaagag gtcaaaaaag acctcatcat taagcttccc aagtactctc
     4621 tctttgagct tgaaaacggc cggaaacgaa tgctcgctag tgcgggcgag ctgcagaaag
     4681 gtaacgagct ggcactgccc tctaaatacg ttaatttctt gtatctggcc agccactatg
     4741 aaaagctcaa aggatctccc gaagataatg agcagaagca gctgttcgtg gaacaacaca
     4801 aacactacct tgatgagatc atcgagcaaa taagcgaatt ctccaaaaga gtgatcctcg
     4861 ccgacgctaa cctcgataag gtgctttctg cttacaataa gcacagggat aagcccatca
     4921 gggagcaggc agaaaacatt atccacttgt ttactctgac caacttgggc gcgcctgcag
     4981 ccttcaagta cttcgacacc accatagaca gaaagcggta cacctctaca aaggaggtcc
     5041 tggacgccac actgattcat cagtcaatta cggggctcta tgaaacaaga atcgacctct
     5101 ctcagctcgg tggagacagc agggctgacc ccaagaagaa gaggaaggtg ggttcgatgg
     5161 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     5221 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     5281 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     5341 tgaccacctt cggctacggc ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc
     5401 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     5461 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     5521 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     5581 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     5641 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     5701 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     5761 tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     5821 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtgagctt
     5881 atactggatt ggtgaatcaa tgagccgtag cacaatggtt acattcggct agctaagatc
     5941 caatggcaag gaccaagtgc tggaacttgt tttgctttag cagatcttag cgtgagaggt
     6001 atttgtcctc tgtcaggagt agatagtaga tgttcttttt aaactaaaat gctaactgtt
     6061 ccgaattcct catcgcagct aatccgtaca tcaaaagaca aaatgctagg tatgtgtact
     6121 acatctcctg ttgctagata agacatatga taggaaacac accatcaata gtcattgtag
     6181 ctttacttat actacgcatt tgcactttcc cctgagtggc agaggcgcat tgagaaaatc
     6241 gatctcaaca tagtttatgt agcatcccct agatccatta ctttaagtct ccttcgtctt
     6301 tggtgtaggc atgttggaca caacgaggta aaacacaaca caaacaatgt gtccagcaaa
     6361 gtagtagctg ctccagttct ccgctactat tgtcttctgc acgaagtggt ttaaactatc
     6421 agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa
     6481 tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca
     6541 accacagggt tccccagatc aggcgctggc tgctgaaccc ccagccggaa ctgaccccac
     6601 aaggccctag cgtttgcaat gcaccaggtc atcattgacc caggcgtgtt ccaccaggcc
     6661 gctgcctcgc aactcttcgc aggcttcgcc gacctgctcg cgccacttct tcacgcgggt
     6721 ggaatccgat ccgcacatga ggcggaaggt ttccagcttg agcgggtacg gctcccggtg
     6781 cgagctgaaa tagtcgaaca tccgtcgggc cgtcggcgac agcttgcggt acttctccca
     6841 tatgaatttc gtgtagtggt cgccagcaaa cagcacgacg atttcctcgt cgatcaggac
     6901 ctggcaacgg gacgttttct tgccacggtc caggacgcgg aagcggtgca gcagcgacac
     6961 cgattccagg tgcccaacgc ggtcggacgt gaagcccatc gccgtcgcct gtaggcgcga
     7021 caggcattcc tcggccttcg tgtaataccg gccattgatc gaccagccca ggtcctggca
     7081 aagctcgtag aacgtgaagg tgatcggctc gccgataggg gtgcgcttcg cgtactccaa
     7141 cacctgctgc cacaccagtt cgtcatcgtc ggcccgcagc tcgacgccgg tgtaggtgat
     7201 cttcacgtcc ttgttgacgt ggaaaatgac cttgttttgc agcgcctcgc gcgggatttt
     7261 cttgttgcgc gtggtgaaca gggcagagcg ggccgtgtcg tttggcatcg ctcgcatcgt
     7321 gtccggccac ggcgcaatat cgaacaagga aagctgcatt tccttgatct gctgcttcgt
     7381 gtgtttcagc aacgcggcct gcttggcctc gctgacctgt tttgccaggt cctcgccggc
     7441 ggtttttcgc ttcttggtcg tcatagttcc tcgcgtgtcg atggtcatcg acttcgccaa
     7501 acctgccgcc tcctgttcaa gacgacgcga acgctccacg gcggccgatg gcgcgggcag
     7561 ggcaggggga gccagttgca cgctgtcgcg ctcgatcttg gccgtagctt gctggaccat
     7621 cgagccgacg gactggaagg tttcgcgggg cgcacgcatg acggtgcggc ttgcgatggt
     7681 ttcggcatcc tcggcggaaa accccgcgtc gatcagttct tgcctgtatg ccttccggtc
     7741 aaacgtccga ttcattcacc ctccttgcgg gattgccccg actcacgccg gggcaatgtg
     7801 cccttattcc tgatttgacc cgcctggtgc cttggtgtcc agataatcca ccttatcggc
     7861 aatgaagtcg gtcccgtaga ccgtctggcc gtccttctcg tacttggtat tccgaatctt
     7921 gccctgcacg aataccagcg accccttgcc caaatacttg ccgtgggcct cggcctgaga
     7981 gccaaaacac ttgatgcgga agaagtcggt gcgctcctgc ttgtcgccgg catcgttgcg
     8041 ccacatctag gatctgccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     8101 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     8161 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     8221 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     8281 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     8341 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     8401 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     8461 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     8521 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     8581 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8641 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8701 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     8761 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8821 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8881 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8941 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agaaccacgc
     9001 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     9061 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     9121 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     9181 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     9241 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     9301 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     9361 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     9421 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     9481 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     9541 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     9601 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     9661 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9721 tttcaatatt attgaagcat ttatcaaggt tattgtctca tgagcggata catatttgaa
     9781 tgtatttaga aaaataaaca aataggggtt ccgcgcacga attggccagc gctgccattt
     9841 ttggggtgag gccgttcgcg gccgaggggc gcagcccctg gggggatggg aggcccgcgt
     9901 tagcgggccg ggagggttcg agaagggggg gcacccccct tcggcgtgcg cggtcacgcg
     9961 cacagggcgc agccctggtt aaaaacaagg tttataaata ttggtttaaa agcaggttaa
    10021 aagacaggtt agcggtggcc gaaaaacggg cggaaaccct tgcaaatgct ggattttctg
    10081 cctgtggaca gcccctcaaa tgtcaatagg tgcgcccctc atctgtcagc actctgcccc
    10141 tcaagtgtca aggatcgcgc ccctcatctg tcagtagtcg cgcccctcaa gtgtcaatac
    10201 cgcagggcac ttatccccag gcttgtccac atcatctgtg ggaaactcgc gtaaaatcag
    10261 gcgttttcgc cgatttgcga ggctggccag ctccacgtcg ccggccgaaa tcgagcctgc
    10321 ccctcatctg tcaacgccgc gccgggtgag tcggcccctc aagtgtcaac gtccgcccct
    10381 catctgtcag tgagggccaa gttttccgcg aggtatccac aacgccggcg gccgcggtgt
    10441 ctcgcacacg gcttcgacgg cgtttctggc gcgtttgcag ggccatagac ggccgccagc
    10501 ccagcggcga gggcaaccag cccggtgagc gtcgcaaagg agatcctgat ctgactgatg
    10561 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
    10621 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
    10681 tgcggacgtt tttaatgtac tggggtggat gcagtgggcc ccac
//
