LOCUS       sequence-169715-       10816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cas9-tracrRNA integration vector for Bacillus subtilis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10816)
  AUTHORS   Westbrook AW, Moo-Young M, Chou CP
  TITLE     Development of a CRISPR-Cas9 toolkit for comprehensive engineering 
            of Bacillus subtilis.
  JOURNAL   Appl Environ Microbiol. 2016 Jun 3. pii: AEM.01159-16.
  PUBMED    27260361
REFERENCE   2  (bases 1 to 10816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2016 Jun 3. pii: AEM.01159-16."
COMMENT     Sequence Label: pAW016-2
FEATURES             Location/Qualifiers
     source          1..10816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(453..4559)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(456..4559)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_RNA        4855..4933
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     promoter        6490..6594
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(6640..6674)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     promoter        7318..7422
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7423..7491,7492..8283)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7641..7660)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8454..9042
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8943..8962
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9196..9213
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9228..9368
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9294..9313)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9454..9476
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(9470..9661)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ccccgatcag tggctggatc ggcccgatat tttagctgac gatatcaaac tgatgaagct
       61 ttctcatacg aatacgtttt ctgtcggcat ttttgcatgg agcgcacttg agccggagga
      121 gggcgtatat caatttgaat ggctggatga tatttttgag cggattcaca gtataggcgg
      181 ccgggtcata ttagcaacgc cgagcggagc ccgtccggcc tggctgtcgc aaacctatcc
      241 ggaagttttg cgcgtcaatg cctcccgcgt caaacagctg cacggcggaa ggcacaacca
      301 ctgcctcaca tctaaagtct accgagaaaa aacacggcac atcaaccgct tattagcaga
      361 acgatacgga catcacccgg cgctgttaat gtggcacatt tcaaacgaat acgggggaga
      421 ttgccactgt gaatcgatgc ggccgctcta gatcagtcac ctcctagctg actcaaatca
      481 atgcgtgttt cataaagacc agtgatggat tgatggataa gagtggcatc taaaacttct
      541 tttgtagacg tatatcgttt acgatcaatt gttgtatcaa aatatttaaa agcagcggga
      601 gctccaagat tcgtcaacgt aaataaatga ataatatttt ctgcttgttc acgtattggt
      661 ttgtctctat gtttgttata tgcactaaga actttatcta aattggcatc tgctaaaata
      721 acacgcttag aaaattcact gatttgctca ataatctcat ctaaataatg cttatgctgc
      781 tccacaaaca attgtttttg ttcgttatct tctggactac ccttcaactt ttcataatga
      841 ctagctaaat ataaaaaatt cacatatttg cttggcagag ccagctcatt tcctttttgt
      901 aattctccgg cactagccag catccgttta cgaccgtttt ctaactcaaa aagactatat
      961 ttaggtagtt taatgattaa gtctttttta acttccttat atcctttagc ttctaaaaag
     1021 tcaatcggat ttttttcaaa ggaacttctt tccataattg tgatccctag taactcttta
     1081 acggatttta acttcttcga tttccctttt tccaccttag caaccactag gactgaataa
     1141 gctaccgttg gactatcaaa accaccatat ttttttggat cccagtcttt tttacgagca
     1201 ataagcttgt ccgaatttct ttttggtaaa attgactcct tggagaatcc gcctgtctgt
     1261 acttctgttt tcttgacaat attgacttgg ggcatggaca atactttgcg cactgtggca
     1321 aaatctcgcc ctttatccca gacaatttct ccagtttccc cattagtttc gattagaggg
     1381 cgtttgcgaa tctctccatt tgcaagtgta atttctgttt tgaagaagtt catgatatta
     1441 gagtaaaaga aatattttgc ggttgctttg cctatttctt gctcagactt agcaatcatt
     1501 ttacgaacat cataaacttt ataatcacca tagacaaact ccgattcaag ttttggatat
     1561 ttcttaatca aagcagttcc aacgacggca tttagatacg catcatgggc atgatggtaa
     1621 ttgttaatct cacgtacttt atagaattgg aaatcttttc ggaagtcaga aactaattta
     1681 gattttaagg taatcacttt aacctctcga ataagtttat cattttcatc gtatttagta
     1741 ttcatgcgac tatccaaaat ttgtgccaca tgcttagtga tttggcgagt ttcaaccaat
     1801 tggcgtttga taaaaccagc tttatcaagt tcactcaaac ctccacgttc agctttcgtt
     1861 aaattatcaa acttacgttg agtgattaac ttggcgttta gaagttgtct ccaatagttt
     1921 ttcatctttt tgactacttc ttcacttgga acgttatccg atttaccacg atttttatca
     1981 gaacgcgtta agaccttatt gtctattgaa tcgtctttaa ggaaactttg tggaacaatg
     2041 tgatcgacat cataatcact taaacgatta atatctaatt cttggtccac atacatgtct
     2101 cttccatttt ggagataata gagatagagc ttttcatttt gcaattgagt attttcaaca
     2161 ggatgctctt taagaatctg acttcctaat tctttgatac cttcttcgat tcgtttcata
     2221 cgctctcgcg aatttttctg gcccttttga gttgtctgat tttcacgtgc catttcaata
     2281 acgatatttt ctggcttatg ccgccccatt actttgacca attcatcaac aacttttaca
     2341 gtctgtaaaa tacctttttt aatagcaggg ctaccagcta aatttgcaat atgttcatgt
     2401 aaactatcgc cttgtccaga cacttgtgct ttttgaatgt cttctttaaa tgtcaaacta
     2461 tcatcatgga tcagctgcat aaaattgcga ttggcaaaac catctgattt caaaaaatct
     2521 aatattgttt tgccagattg cttatcccta ataccattaa tcaattttcg agacaaacgt
     2581 ccccaaccag tataacggcg acgtttaagc tgtttcatca ccttatcatc aaagaggtga
     2641 gcatatgttt taagtctttc ctcaatcatc tccctatctt caaataaggt caatgttaaa
     2701 acaatatcct ctaagatatc ttcattttct tcattatcca aaaaatcttt atctttaata
     2761 atttttagca aatcatggta ggtacctaat gaagcattaa atctatcttc aactcctgaa
     2821 atttcaacac tatcaaaaca ttctattttt ttgaaataat cttcttttaa ttgcttaacg
     2881 gttacttttc gatttgtttt gaagagtaaa tcaacaatgg ctttcttctg ttcacctgaa
     2941 agaaatgctg gttttcgcat tccttcagta acatatttga cctttgtcaa ttcgttataa
     3001 accgtaaaat actcataaag caaactatgt tttggtagta ctttttcatt tggaagattt
     3061 ttatcaaagt ttgtcatgcg ttcaataaat gattgagctg aagcaccttt atcgacaact
     3121 tcttcaaaat tccatggggt aattgtttct tcagacttcc gagtcatcca tgcaaaacga
     3181 ctattgccac gcgccaatgg accaacataa taaggaattc gaaaagtcaa gattttttca
     3241 atcttctcac gattgtcttt taaaaatgga taaaagtctt cttgtcttct caaaatagca
     3301 tgcagctcac ccaagtgaat ttgatgggga atagagccgt tgtcaaaggt ccgttgcttg
     3361 cgcagcaaat cttcacgatt tagtttcacc aataattcct cagtaccatc cattttttct
     3421 aaaattggtt tgataaattt ataaaattct tcttggctag ctcccccatc aatataacct
     3481 gcatatccgt tttttgattg atcaaaaaag atttctttat acttttctgg aagttgttgt
     3541 cgaactaaag cttttaaaag agtcaagtct tgatgatgtt catcgtagcg tttaatcatt
     3601 gaagctgata ggggagcctt agttatttca gtatttactc ttaggatatc tgaaagtaaa
     3661 atagcatctg ataaattctt agctgccaaa aacaaatcag catattgatc tccaatttgc
     3721 gccaataaat tatctaaatc atcatcgtaa gtatcttttg aaagctgtaa tttagcatct
     3781 tctgccaaat caaaatttga tttaaaatta ggggtcaaac ccaatgacaa agcaatgaga
     3841 ttcccaaata agccattttt cttctcaccg gggagctgag caatgagatt ttctaatcgt
     3901 cttgatttac tcaatcgtgc agaaagaatc gctttagcat ctactccact tgcgttaata
     3961 gggttttctt caaataattg attgtaggtt tgtaccaact ggataaatag tttgtccaca
     4021 tcactattat caggatttaa atctccctca atcaaaaaat gaccacgaaa cttaatcata
     4081 tgcgctaagg ccaaatagat taagcgcaaa tccgctttat cagtagaatc taccaatttt
     4141 tttcgcagat gatagatagt tggatatttc tcatgataag caacttcatc tactatattt
     4201 ccaaaaatag gatgacgttc atgcttcttg tcttcttcca ccaaaaaaga ctcttcaagt
     4261 cgatgaaaga aactatcatc tactttcgcc atctcatttg aaaaaatctc ctgtagataa
     4321 caaatacgat tcttccgacg tgtatacctt ctacgagctg tccgtttgag acgagtcgct
     4381 tccgctgtct ctccactgtc aaataaaaga gcccctataa gatttttttt gatactgtgg
     4441 cggtctgtat ttcccagaac cttgaacttt ttagacggaa ccttatattc atcagtgatc
     4501 accgcccatc cgacgctatt tgtgccgata tctaagccta ttgagtattt cttatccatt
     4561 tttgcctcct aaaataaaaa gtttaaatta aatccataat gagtttgatg atttcaataa
     4621 tagttttaat gacctccgaa attagtttaa tatgctttaa tttttctttt tcaaaatatc
     4681 tcttcaaaaa atattaccca atacttaata ataaatagat tataacacaa aattctttta
     4741 aaaagtagtt tattttgtta tcattctata gtattaagta ttgttttatg gctgataaat
     4801 ttctttgaat ttctccttga ttatttgtta taaaagttat aaaataatct tgttggaacc
     4861 attcaaaaca gcatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
     4921 accgagtcgg tgcttttttt gatacttcta ttctactctg actgcaaacc aaaaaaacaa
     4981 gcgctttcaa aacgcttgtt ttatcatttt tagggaaatt aatctcttaa tccttttatc
     5041 attctacatt taggcgctgc catcttgcta aacctactaa gctccacagg atgatttcgt
     5101 aatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg
     5161 tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc
     5221 gttagcaatt taactgtgat aaactaccgc attatctaga gtctagggac ctctttagct
     5281 ccttggaagc tgtcagtagt atacctaata atttatctac attcccttta gtaacgtgta
     5341 actttccaaa tttacaaaag cgactcatag aattatttcc tcccgttaaa taatagataa
     5401 ctattaaaaa tagacaatac ttgctcataa gtaacggtac ttaaattgtt tactttggcg
     5461 tgtttcattg cttgatgaaa ctgattttta gtaaacagtt gacgatattc tcgattgacc
     5521 cattttgaaa caaagtacgt atatagcttc caatatttat ctggaacatc tgtggtatgg
     5581 cgggtaagtt ttattaagac actgtttact tttggtttag gatgaaagca ttccgctggc
     5641 agcttaagca attgctgaat cgagacttga gtgtgcaaga gcaaccctag tgttcggtga
     5701 atatccaagg tacgcttgta gaatccttct tcaacaatca gatagatgtc agacgcatgg
     5761 ctttcaaaaa ccactttttt aataatttgt gtgcttaaat ggtaaggaat actcccaaca
     5821 attttatacc tctgtttgtt agggaattga aactgtagaa tatcttggtg aattaaagtg
     5881 acacgagtat tcagttttaa tttttctgac gataagttga atagatgact gtctaattca
     5941 atagacgtta cctgtttact tattttagcc agtttcgtcg ttaaatgccc tttacctgtt
     6001 ccaatttcgt aaacggtatc ggtttctttt aaattcaatt gttttattat ttggttgagt
     6061 actttttcac tcgttaaaaa gttttgagaa tattttatat ttttgttcat gtaatcactc
     6121 cttcttaatt acaaattttt agcatctaat ttaacttcaa ttcctattat acaaaatttt
     6181 aagatactgc actatcaaca cactcttaag tttgcttcta agtcttattt ccataacttc
     6241 ttttacgttt ccgccattct ttgctgtttc gatttttatg atatggtgca agtcagcacg
     6301 aacacgaacc gtcttatctc ccattatatc tttttttgca ctgattggtg tatcatttcg
     6361 tttttctttt tgtgctagag gatcaattct tgaagacgaa agggcctcgt gatacgccta
     6421 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     6481 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     6541 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtgcggcc
     6601 gcccgcggga gctccccggg acgttcttgc cattgctgca taaaaaacgc ccggcggcaa
     6661 ccgagcgttc tgaattaatt aatcatcgcg actgcagaga tatcgatttc aagctatatt
     6721 tggagttgag cctcttgaaa cggacaccct gtatccgaag gatcgaaacg ctgtcagcta
     6781 ccgcagccaa atatatgaaa tgaaggatta tgcaaccgtg attgatgtaa agacagcttc
     6841 agtggaagcg gtgtatcaag aagattttta tgcgcgcacg ccagcggtca caagccatga
     6901 gtatcagcag ggcaaggcgt attttatcgg cgcgcgtttg gaggatcaat ttcagcgtga
     6961 tttctatgag ggtctgatca cagacctgtc tctctctcca gtttttccgg ttcggcacgg
     7021 aaaaggcgtc tccgtacaag cgaggcagga tcaggacaat gattatattt ttgtcatgaa
     7081 tttcacggaa gaaaaacagc tggtcacgtt tgatcagagt gtgaaggaca taatgacagg
     7141 agacatattg tcaggcgacc tgacgatgga aaagtatgaa gtgagaattg tcgtaaacac
     7201 acattagccc atcattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     7261 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     7321 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     7381 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     7441 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     7501 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     7561 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     7621 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     7681 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     7741 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     7801 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     7861 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     7921 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca
     7981 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     8041 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     8101 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     8161 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     8221 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     8281 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     8341 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     8401 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     8461 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     8521 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     8581 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     8641 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     8701 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     8761 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     8821 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     8881 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     8941 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     9001 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     9061 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     9121 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     9181 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat
     9241 tttctcctta cgcatctgtg cggtatttca taccgcatat ggtgcactct cagtacaatc
     9301 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
     9361 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     9421 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     9481 caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa
     9541 gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg
     9601 ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca
     9661 ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag
     9721 agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg
     9781 agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat
     9841 gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga
     9901 tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa
     9961 cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt
    10021 cgcttcacgt tcgctcgcgt atcggtggtt cattctgcta accagtaagg caaccccgcc
    10081 agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa
    10141 cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct
    10201 gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg
    10261 gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct
    10321 ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca
    10381 tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc
    10441 aatgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc
    10501 gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc
    10561 gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgactaaga aaatgccgtc
    10621 aaatccgctc gccatgactt cactaacgat gcctttgaaa atcttcaagt tctcttctac
    10681 taattcaagg cgtgtctcac caggtttttg gtttgctccg gcgcaaatgc agacaatatc
    10741 aggatggggt gatgtcaaag cttgaaaaaa cgcacgtaac aaaagcaaaa tttatgctcc
    10801 atgggggaga ctacaa
//
