LOCUS       sequence-169722-         492 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses calcium-dependent Sortase A with a TEV cleavage site 
            proximal to the C-terminal histidine tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Sarpong K, Bose R
  TITLE     Efficient sortase-mediated N-terminal labeling of TEV protease 
            cleaved recombinant proteins.
  JOURNAL   Anal Biochem. 2017 Mar 15;521:55-58. doi: 10.1016/j.ab.2017.01.008. 
            Epub 2017 Jan 11.
  PUBMED    28088451
REFERENCE   2  (bases 1 to 492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem."; date: "2017-03-15"; volume: "521:55-58. doi"; pages: " 
            10.1016/j.ab.2017.01.008. Epub 2017 Jan 11"
COMMENT     Sequence Label: pET30b-5M SrtA with TEV site
FEATURES             Location/Qualifiers
     source          1..492
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             451..471
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             472..489
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgcaagcta aacctcaaat tccgaaagat aaatcaaaag tggcaggcta tattgaaatt
       61 ccagatgctg atattaaaga accagtatat ccaggaccag caacacgcga acaattaaat
      121 agaggtgtaa gctttgcaga agaaaatgaa tcactagatg atcaaaatat ttcaattgca
      181 ggacacactt tcattgaccg tccgaactat caatttacaa atcttaaagc agccaaaaaa
      241 ggtagtatgg tgtactttaa agttggtaat gaaacacgta agtataaaat gacaagtata
      301 agaaacgtta agccaacagc tgtagaagtt ctagatgaac aaaaaggtaa agataaacaa
      361 ttaacattaa ttacttgtga tgattacaat gaagagacag gcgtttggga aacacgtaaa
      421 atctttgtag ctacagaagt caaactcgag gagaatctct attttcaagg tcaccaccac
      481 caccaccact ga
//
