LOCUS       sequence-169739-       13398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  genomic Sindbis virus plasmid for expression of SYNseq components.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Peikon ID, Kebschull JM, Vagin VV, Ravens DI, Sun YC, Brouzes E, 
            Correa IR Jr, Bressan D, Zador AM
  TITLE     Using high-throughput barcode sequencing to efficiently map 
            connectomes.
  JOURNAL   Nucleic Acids Res. 2017 Jul 7;45(12):e115. doi: 10.1093/nar/gkx292.
  PUBMED    28449067
REFERENCE   2  (bases 1 to 13398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2017-07-7"; volume: "45(12):e115. doi"; pages: "
            10.1093/nar/gkx292"
COMMENT     Sequence Label: IDP466
FEATURES             Location/Qualifiers
     source          1..13398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             7804..7833
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             7846..7911
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             7918..8463
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylcytosine 
                     derivatives"
                     /label=CLIP-tag(TM)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLIQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISESHLAALVGNPAATAAVNTALDGNPVPILIPCHRVVQGDSDVGPYLGGLA
                     VKEWLLAHEGHRLGKPGLG"
     regulatory      9580..9589
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9586..9651
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     gap             10132..10161
                     /estimated_length=30
     regulatory      10226..10235
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(10232..10234,10235..10237,10238..10951)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(10232..10234,10235..10237,10238..10948)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(10277..10298)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10538..10557)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10885..10906
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        11569..11673
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11674..11742,11743..12534)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11892..11911)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12705..13293
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13194..13213
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 attgacggcg tagtacacac tattgaatca aacagccgac caattgcact accatcacaa
       61 tggagaagcc agtagtaaac gtagacgtag acccccagag tccgtttgtc gtgcaactgc
      121 aaaaaagctt cccgcaattt gaggtagtag cacagcaggt cactccaaat gaccatgcta
      181 atgccagagc attttcgcat ctggccagta aactaatcga gctggaggtt cctaccacag
      241 cgacgatctt ggacataggc agcgcaccgg ctcgtagaat gttttccgag caccagtatc
      301 attgtgtctg ccccatgcgt agtccagaag acccggaccg catgatgaaa tacgccagta
      361 aactggcgga aaaagcgtgc aagattacaa acaagaactt gcatgagaag attaaggatc
      421 tccggaccgt acttgatacg ccggatgctg aaacaccatc gctctgcttt cacaacgatg
      481 ttacctgcaa catgcgtgcc gaatattccg tcatgcagga cgtgtatatc aacgctcccg
      541 gaactatcta tcatcaggct atgaaaggcg tgcggaccct gtactggatt ggcttcgaca
      601 ccacccagtt catgttctcg gctatggcag gttcgtaccc tgcgtacaac accaactggg
      661 ccgacgagaa agtccttgaa gcgcgtaaca tcggactttg cagcacaaag ctgagtgaag
      721 gtaggacagg aaaattgtcg ataatgagga agaaggagtt gaagcccggg tcgcgggttt
      781 atttctccgt aggatcgaca ctttatccag aacacagagc cagcttgcag agctggcatc
      841 ttccatcggt gttccacttg aatggaaagc agtcgtacac ttgccgctgt gatacagtgg
      901 tgagttgcga aggctacgta gtgaagaaaa tcaccatcag tcccgggatc acgggagaaa
      961 ccgtgggata cgcggttaca cacaatagcg agggcttctt gctatgcaaa gttactgaca
     1021 cagtaaaagg agaacgggta tcgttccctg tgtgcacgta catcccggcc accatatgcg
     1081 atcagatgac tggtataatg gccacggata tatcacctga cgatgcacaa aaacttctgg
     1141 ttgggctcaa ccagcgaatt gtcattaacg gtaggactaa caggaacacc aacaccatgc
     1201 aaaattacct tctgccgatc atagcacaag ggttcagcaa atgggctaag gagcgcaagg
     1261 atgatcttga taacgagaaa atgctgggta ctagagaacg caagcttacg tatggctgct
     1321 tgtgggcgtt tcgcactaag aaagtacatt cgttttatcg cccacctgga acgcagacct
     1381 gcgtaaaagt cccagcctct tttagcgctt ttcccatgtc gtccgtatgg acgacctctt
     1441 tgcccatgtc gctgaggcag aaattgaaac tggcattgca accaaagaag gaggaaaaac
     1501 tgctgcaggt ctcggaggaa ttagtcatgg aggccaaggc tgcttttgag gatgctcagg
     1561 aggaagccag agcggagaag ctccgagaag cacttccacc attagtggca gacaaaggca
     1621 tcgaggcagc cgcagaagtt gtctgcgaag tggaggggct ccaggcggac atcggagcag
     1681 cattagttga aaccccgcgc ggtcacgtaa ggataatacc tcaagcaaat gaccgtatga
     1741 tcggacagta tatcgttgtc tcgccaaact ctgtgctgaa gaatgccaaa ctcgcaccag
     1801 cgcacccgct agcagatcag gttaagatca taacacactc cggaagatca ggaaggtacg
     1861 cggtcgaacc atacgacgct aaagtactga tgccagcagg aggtgccgta ccatggccag
     1921 aattcctagc actgagtgag agcgccacgt tagtgtacaa cgaaagagag tttgtgaacc
     1981 gcaaactata ccacattgcc atgcatggcc ccgccaagaa tacagaagag gagcagtaca
     2041 aggttacaaa ggcagagctt gcagaaacag agtacgtgtt tgacgtggac aagaagcgtt
     2101 gcgttaagaa ggaagaagcc tcaggtctgg tcctctcggg agaactgacc aaccctccct
     2161 atcatgagct agctctggag ggactgaaga cccgacctgc ggtcccgtac aaggtcgaaa
     2221 caataggagt gataggcaca ccggggtcgg gcaagtcagc tattatcaag tcaactgtca
     2281 cggcacgaga tcttgttacc agcggaaaga aagaaaattg tcgcgaaatt gaggccgacg
     2341 tgctaagact gaggggtatg cagattacgt cgaagacagt agattcggtt atgctcaacg
     2401 gatgccacaa agccgtagaa gtgctgtacg ttgacgaagc gttcgcgtgc cacgcaggag
     2461 cactacttgc cttgattgct atcgtcaggc cccgcaagaa ggtagtacta tgcggagacc
     2521 ccatgcaatg cggattcttc aacatgatgc aactaaaggt acatttcaat caccctgaaa
     2581 aagacatatg caccaagaca ttctacaagt atatctcccg gcgttgcaca cagccagtta
     2641 cagctattgt atcgacactg cattacgatg gaaagatgaa aaccacgaac ccgtgcaaga
     2701 agaacattga aatcgatatt acaggggcca caaagccgaa gccaggggat atcatcctga
     2761 catgtttccg cgggtgggtt aagcaattgc aaatcgacta tcccggacat gaagtaatga
     2821 cagccgcggc ctcacaaggg ctaaccagaa aaggagtgta tgccgtccgg caaaaagtca
     2881 atgaaaaccc actgtacgcg atcacatcag agcatgtgaa cgtgttgctc acccgcactg
     2941 aggacaggct agtgtggaaa accttgcagg gcgacccatg gattaagcag cccactaaca
     3001 tacctaaagg aaactttcag gctactatag aggactggga agctgaacac aagggaataa
     3061 ttgctgcaat aaacagcccc actccccgtg ccaatccgtt cagctgcaag accaacgttt
     3121 gctgggcgaa agcattggaa ccgatactag ccacggccgg tatcgtactt accggttgcc
     3181 agtggagcga actgttccca cagtttgcgg atgacaaacc acattcggcc atttacgcct
     3241 tagacgtaat ttgcattaag tttttcggca tggacttgac aagcggactg ttttctaaac
     3301 agagcatccc actaacgtac catcccgccg attcagcgag gccggtagct cattgggaca
     3361 acagcccagg aacccgcaag tatgggtacg atcacgccat tgccgccgaa ctctcccgta
     3421 gatttccggt gttccagcta gctgggaagg gcacacaact tgatttgcag acggggagaa
     3481 ccagagttat ctctgcacag cataacctgg tcccggtgaa ccgcaatctt cctcacgcct
     3541 tagtccccga gtacaaggag aagcaacccg gcccggtcaa aaaattcttg aaccagttca
     3601 aacaccactc agtacttgtg gtatcagagg aaaaaattga agctccccgt aagagaatcg
     3661 aatggatcgc cccgattggc atagccggtg cagataagaa ctacaacctg gctttcgggt
     3721 ttccgccgca ggcacggtac gacctggtgt tcatcaacat tggaactaaa tacagaaacc
     3781 accactttca gcagtgcgaa gaccatgcgg cgaccttaaa aaccctttcg cgttcggccc
     3841 tgaattgcct taacccagga ggcaccctcg tggtgaagtc ctatggctac gccgaccgca
     3901 acagtgagga cgtagtcacc gctcttgcca gaaagtttgt cagggtgtct gcagcgagac
     3961 cagattgtgt ctcaagcaat acagaaatgt acctgatttt ccgacaacta gacaacagcc
     4021 gtacacggca attcaccccg caccatctga attgcgtgat ttcgtccgtg tatgagggta
     4081 caagagatgg agttggagcc gcgccgtcat accgcaccaa aagggagaat attgctgact
     4141 gtcaagagga agcagttgtc aacgcagcca atccgctggg tagaccaggc gaaggagtct
     4201 gccgtgccat ctataaacgt tggccgacca gttttaccga ttcagccacg gagacaggca
     4261 ccgcaagaat gactgtgtgc ctaggaaaga aagtgatcca cgcggtcggc cctgatttcc
     4321 ggaagcaccc agaagcagaa gccttgaaat tgctacaaaa cgcctaccat gcagtggcag
     4381 acttagtaaa tgaacataac atcaagtctg tcgccattcc actgctatct acaggcattt
     4441 acgcagccgg aaaagaccgc cttgaagtat cacttaactg cttgacaacc gcgctagaca
     4501 gaactgacgc ggacgtaacc atctattgcc tggataagaa gtggaaggaa agaatcgacg
     4561 cggcactcca acttaaggag tctgtaacag agctgaagga tgaagatatg gagatcgacg
     4621 atgagttagt atggattcat ccagacagtt gcttgaaggg aagaaaggga ttcagtacta
     4681 caaaaggaaa attgtattcg tacttcgaag gcaccaaatt ccatcaagca gcaaaagaca
     4741 tggcggagat aaaggtcctg ttccctaatg accaggaaag taatgaacaa ctgtgtgcct
     4801 acatattggg tgagaccatg gaagcaatcc gcgaaaagtg cccggtcgac cataacccgt
     4861 cgtctagccc gcccaaaacg ttgccgtgcc tttgcatgta tgccatgacg ccagaaaggg
     4921 tccacagact tagaagcaat aacgtcaaag aagttacagt atgctcctcc accccccttc
     4981 ctaagcacaa aattaagaat gttcagaagg ttcagtgcac gaaagtagtc ctgtttaatc
     5041 cgcacactcc cgcattcgtt cccgcccgta agtacataga agtgccagaa cagcctaccg
     5101 ctcctcctgc acaggccgag gaggcccccg aagttgtagc gacaccgtca ccatctacag
     5161 ctgataacac ctcgcttgat gtcacagaca tctcactgga tatggatgac agtagcgaag
     5221 gctcactttt ttcgagcttt agcggatcgg acaactctat tactagtatg gacagttggt
     5281 cgtcaggacc tagttcacta gagatagtag accgaaggca ggtggtggtg gctgacgttc
     5341 atgccgtcca agagcctgcc cctattccac cgccaaggct aaagaagatg gcccgcctgg
     5401 cagcggcaag aaaagagccc actccaccgg caagcaatag ctctgagtcc ctccacctct
     5461 cttttggtgg ggtatccatg tccctcggat caattttcga cggagagacg gcccgccagg
     5521 cagcggtaca acccctggca acaggcccca cggatgtgcc tatgtctttc ggatcgtttt
     5581 ccgacggaga gattgatgag ctgagccgca gagtaactga gtccgaaccc gtcctgtttg
     5641 gatcatttga accgggcgaa gtgaactcaa ttatatcgtc ccgatcagcc gtatcttttc
     5701 cactacgcaa gcagagacgt agacgcagga gcaggaggac tgaatactga ctaaccgggg
     5761 taggtgggta catattttcg acggacacag gccctgggca cttgcaaaag aagtccgttc
     5821 tgcagaacca gcttacagaa ccgaccttgg agcgcaatgt cctggaaaga attcatgccc
     5881 cggtgctcga cacgtcgaaa gaggaacaac tcaaactcag gtaccagatg atgcccaccg
     5941 aagccaacaa aagtaggtac cagtctcgta aagtagaaaa tcagaaagcc ataaccactg
     6001 agcgactact gtcaggacta cgactgtata actctgccac agatcagcca gaatgctata
     6061 agatcaccta tccgaaacca ttgtactcca gtagcgtacc ggcgaactac tccgatccac
     6121 agttcgctgt agctgtctgt aacaactatc tgcatgagaa ctatccgaca gtagcatctt
     6181 atcagattac tgacgagtac gatgcttact tggatatggt agacgggaca gtcgcctgcc
     6241 tggatactgc aaccttctgc cccgctaagc ttagaagtta cccgaaaaaa catgagtata
     6301 gagccccgaa tatccgcagt gcggttccat cagcgatgca gaacacgcta caaaatgtgc
     6361 tcattgccgc aactaaaaga aattgcaacg tcacgcagat gcgtgaactg ccaacactgg
     6421 actcagcgac attcaatgtc gaatgctttc gaaaatatgc atgtaatgac gagtattggg
     6481 aggagttcgc tcggaagcca attaggatta ccactgagtt tgtcaccgca tatgtagcta
     6541 gactgaaagg ccctaaggcc gccgcactat ttgcaaagac gtataatttg gtcccattgc
     6601 aagaagtgcc tatggataga ttcgtcatgg acatgaaaag agacgtgaaa gttacaccag
     6661 gcacgaaaca cacagaagaa agaccgaaag tacaagtgat acaagccgca gaacccctgg
     6721 cgactgctta cttatgcggg attcaccggg aattagtgcg taggcttacg gccgtcttgc
     6781 ttccaaacat tcacacgctt tttgacatgt cggcggagga ttttgatgca atcatagcag
     6841 aacacttcaa gcaaggcgac ccggtactgg agacggatat cgcatcattc gacaaaagcc
     6901 aagacgacgc tatggcgtta accggtctga tgatcttgga ggacctgggt gtggatcaac
     6961 cactactcga cttgatcgag tgcgcctttg gagaaatatc atccacccat ctacctacgg
     7021 gtactcgttt taaattcggg gcgatgatga aatccggaat gttcctcaca ctttttgtca
     7081 acacagtttt gaatgtcgtt atcgccagca gagtactaga agagcggctt aaaacgtcca
     7141 gatgtgcagc gttcattggc gacgacaaca tcatacatgg agtagtatct gacaaagaaa
     7201 tggctgagag gtgcgccacc tggctcaaca tggaggttaa gatcatcgac gcagtcatcg
     7261 gtgagagacc accttacttc tgcggcggat ttatcttgca agattcggtt acttccacag
     7321 cgtgccgcgt ggcggatccc ctgaaaaggc tgtttaagtt gggtaaaccg ctcccagccg
     7381 acgacgagca agacgaagac agaagacgcg ctctgctaga tgaaacaaag gcgtggttta
     7441 gagtaggtat aacaggcact ttagcagtgg ccgtgacgac ccggtatgag gtagacaata
     7501 ttacacctgt cctactggca ttgagaactt ttgcccagag caaaagagca ttccaagcca
     7561 tcagagggga aattaaagca tctctacggt ggtcctaaat agtcagcata gtacatttca
     7621 tctgactaat accacaacac caccacctct agagccacca tgtaccagag gatgctccgg
     7681 tgcggcgccg atctgggatc gcccgggggc ggcagtggcg gcggcgcagg ggggcgcctg
     7741 gccctgatct ggatagtccc gctcaccctc ggcggcctcc taggagtggc ctggggggaa
     7801 ttcgaacaaa aactcatctc agaagaggat ctacaattcg gcggagacta caaagaccat
     7861 gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa gggagctatg
     7921 gacaaagact gcgaaatgaa gcgcaccacc ctggatagcc ctctgggcaa gctggaactg
     7981 tctgggtgcg aacagggcct gcaccgtatc atcttcctgg gcaaaggaac atctgccgcc
     8041 gacgccgtgg aagtgcctgc cccagccgcc gtgctgggcg gaccagagcc actgatccag
     8101 gccaccgcct ggctcaacgc ctactttcac cagcctgagg ccatcgagga gttccctgtg
     8161 ccagccctgc accacccagt gttccagcag gagagcttta cccgccaggt gctgtggaaa
     8221 ctgctgaaag tggtgaagtt cggagaggtc atcagcgaga gccacctggc cgccctggtg
     8281 ggcaatcccg ccgccaccgc cgccgtgaac accgccctgg acggaaatcc cgtgcccatt
     8341 ctgatcccct gccaccgggt ggtgcagggc gacagcgacg tggggcccta cctgggcggg
     8401 ctcgccgtga aagagtggct gctggcccac gagggccaca gactgggcaa gcctgggctg
     8461 ggtgtcgacg gtaccgcatc cagtttggga gcgcaccaca tccaccattt ccatggcagc
     8521 agcaagcatc attcagtgcc tattgcaatc tacaggtcac cagcatcctt gcgaggcgga
     8581 cacgctggga cgacgtatat ctttagcaaa ggtggtggac aaattacata taagtggcct
     8641 cctaatgacc gacccagtac acgagcagac aggctggcca tcgggtttag cactgttcag
     8701 aaggaagcag tgttggtgcg tgtggacagt tcctcaggcc tgggtgacta ccttgagctg
     8761 cacatacacc aaggaaaaat tggagttaaa tttaatgttg ggacagatga catcgccatt
     8821 gaggagtcca atgcaatcat taatgatgga aaataccatg tagtgcgttt cacgaggagt
     8881 ggtggcaatg ccacgttaca ggtggacagc tggccagtca tcgaacgata ccctgcaggg
     8941 cgtcagctca caatcttcaa tagccaagca accataataa ttggcgggaa agagcagggc
     9001 cagcccttcc agggccagct ctctgggctt tactacaatg gcttgaaagt tctgaatatg
     9061 gcggcagaga acgatgccaa catcgccata gtgggaaatg tgaggctggt cggtgaagtg
     9121 ccttcctcta tgacaactga gtcgacagcc actgccatgc agtccgagat gtccacctca
     9181 atcatggaga ccaccacaac cctggctacc agcacagctc gacgaggaaa gccccccaca
     9241 aaggagccta tcagccagac cacggatgat atccttgtgg cctcggcaga gtgtcccagc
     9301 gacgatgagg acattgaccc ctgtgagccg agctcaggtg ggttagccaa ccccacccga
     9361 gtgggcggac gcgaaccata cccaggctcg gcagaggtga tccgggagtc tagcagtacc
     9421 actggcatgg tggtggggat tgtcgcagca gcagctctgt gcatcctcat cctcctctat
     9481 gccatgtaca agtacaggaa ccgggatgaa gggtcgagcg ctccaccgct cgacggagcc
     9541 ggagctggcg ctggagctgg agccggagct ggcgggctag ccaccatgga cgcacaaaca
     9601 cgacgacgtg agcgtcgcgc tgagaaacaa gctcaatgga aagctgcaaa cctcgagcca
     9661 ccgctcgacg gagccggagc tggcgctgga gctggagccg gagctggcgg tctagccacc
     9721 ccaccgagcg cttaccacgt ggatgagagt cgaaactaca tcagtaactc agcacagtcc
     9781 aatggggctg tggtcaagga gatgcaaccc agcagtgcta aaagcgccaa caaaaacaag
     9841 aagaacaagg ataaggagta ttatgtctag gcatgcaggc cttaaagcat ctctacggtg
     9901 gtcctaaata gtcagcatag tacatttcat ctgactaata ccacaacacc accacctcta
     9961 gtagaggcgc cggacgatgc cgtcctcgta gttcgggcat gtactggagc cgagaggtaa
    10021 cctcattata atcgttcgct attcagggat tgaccaacac cggaaaacat ctcacttgaa
    10081 gtaatatata cgacagagtc gcggccgcaa ccggtggaca cgtcttatgt gnnnnnnnnn
    10141 nnnnnnnnnn nnnnnnnnnn nagatcggaa gagcgtcgtg tagggaaaga gtgtagatct
    10201 cggtggtcgc cgtatcatta cgcgtgccac catggtgagc aagggcgagg agctgttcac
    10261 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
    10321 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
    10381 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
    10441 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
    10501 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
    10561 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
    10621 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
    10681 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
    10741 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
    10801 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
    10861 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
    10921 cactctcggc atggacgagc tgtacaagta aagatcttac ctaattgccg tcgtgaggta
    10981 cgaccaccgc tagctgtaca gccctgaaaa agggctcgag ccctgaaaaa gggcaattgc
    11041 cctgaaaaag ggcgtcgacg ccctgaaaaa gggcggaatt catgtccaat ttactgactg
    11101 tacaccaaaa tttgcctgct accgcgggcc caatgatccg accagcaaaa ctcgatgtac
    11161 ttccgaggaa ctgatgtgca taatgcatca ggctggtaca ttagatcccc gcttaccgcg
    11221 ggcaatatag caacactaaa aactcgatgt acttccgagg aagcgcagtg cataatgttg
    11281 cgcagtgttg ccacataacc actatattaa ccatttattt agcggacgcc aaaaactcaa
    11341 tgtatttttg aggaagcgtg gtgcataatg ccacgcaggt tttgcataat tttttttttt
    11401 ttttttatta atcaacaaaa ttttgttttt aacatttcaa aaaaaaaaaa aaaaaaaaaa
    11461 aaaaaaaaaa aaggggaatt cctcgataat taagcgaccg cttaattaat cgaggggaat
    11521 taattcttga agacgaaagg gccaggtggc acttttcggg gaaatgtgcg cggaacccct
    11581 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
    11641 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
    11701 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
    11761 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
    11821 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
    11881 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
    11941 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
    12001 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
    12061 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
    12121 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
    12181 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
    12241 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
    12301 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
    12361 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
    12421 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
    12481 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
    12541 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
    12601 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    12661 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    12721 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    12781 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    12841 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    12901 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    12961 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    13021 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    13081 tacctacagc gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    13141 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    13201 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    13261 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcgag ctcgtatgga
    13321 catattgtcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
    13381 atttagggga cactatag
//
