LOCUS       sequence-169757-       14085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  genomic Sindbis virus plasmid for use in SYNseq PLA screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14085)
  AUTHORS   Peikon ID, Kebschull JM, Vagin VV, Ravens DI, Sun YC, Brouzes E, 
            Correa IR Jr, Bressan D, Zador AM
  TITLE     Using high-throughput barcode sequencing to efficiently map 
            connectomes.
  JOURNAL   Nucleic Acids Res. 2017 Jul 7;45(12):e115. doi: 10.1093/nar/gkx292.
  PUBMED    28449067
REFERENCE   2  (bases 1 to 14085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2017-07-7"; volume: "45(12):e115. doi"; pages: "
            10.1093/nar/gkx292"
COMMENT     Sequence Label: IDP425
FEATURES             Location/Qualifiers
     source          1..14085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7654..7663
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7801..7827
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7834..8379
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     regulatory      8841..8850
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      10636..10645
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10642..10707
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     regulatory      11093..11102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             11099..11812
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYKS"
     primer_bind     complement(11244..11262)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     11512..11531
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        12256..12360
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12361..12429,12430..13221)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12579..12598)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13392..13980
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13881..13900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 attgacggcg tagtacacac tattgaatca aacagccgac caattgcact accatcacaa
       61 tggagaagcc agtagtaaac gtagacgtag acccccagag tccgtttgtc gtgcaactgc
      121 aaaaaagctt cccgcaattt gaggtagtag cacagcaggt cactccaaat gaccatgcta
      181 atgccagagc attttcgcat ctggccagta aactaatcga gctggaggtt cctaccacag
      241 cgacgatctt ggacataggc agcgcaccgg ctcgtagaat gttttccgag caccagtatc
      301 attgtgtctg ccccatgcgt agtccagaag acccggaccg catgatgaaa tacgccagta
      361 aactggcgga aaaagcgtgc aagattacaa acaagaactt gcatgagaag attaaggatc
      421 tccggaccgt acttgatacg ccggatgctg aaacaccatc gctctgcttt cacaacgatg
      481 ttacctgcaa catgcgtgcc gaatattccg tcatgcagga cgtgtatatc aacgctcccg
      541 gaactatcta tcatcaggct atgaaaggcg tgcggaccct gtactggatt ggcttcgaca
      601 ccacccagtt catgttctcg gctatggcag gttcgtaccc tgcgtacaac accaactggg
      661 ccgacgagaa agtccttgaa gcgcgtaaca tcggactttg cagcacaaag ctgagtgaag
      721 gtaggacagg aaaattgtcg ataatgagga agaaggagtt gaagcccggg tcgcgggttt
      781 atttctccgt aggatcgaca ctttatccag aacacagagc cagcttgcag agctggcatc
      841 ttccatcggt gttccacttg aatggaaagc agtcgtacac ttgccgctgt gatacagtgg
      901 tgagttgcga aggctacgta gtgaagaaaa tcaccatcag tcccgggatc acgggagaaa
      961 ccgtgggata cgcggttaca cacaatagcg agggcttctt gctatgcaaa gttactgaca
     1021 cagtaaaagg agaacgggta tcgttccctg tgtgcacgta catcccggcc accatatgcg
     1081 atcagatgac tggtataatg gccacggata tatcacctga cgatgcacaa aaacttctgg
     1141 ttgggctcaa ccagcgaatt gtcattaacg gtaggactaa caggaacacc aacaccatgc
     1201 aaaattacct tctgccgatc atagcacaag ggttcagcaa atgggctaag gagcgcaagg
     1261 atgatcttga taacgagaaa atgctgggta ctagagaacg caagcttacg tatggctgct
     1321 tgtgggcgtt tcgcactaag aaagtacatt cgttttatcg cccacctgga acgcagacct
     1381 gcgtaaaagt cccagcctct tttagcgctt ttcccatgtc gtccgtatgg acgacctctt
     1441 tgcccatgtc gctgaggcag aaattgaaac tggcattgca accaaagaag gaggaaaaac
     1501 tgctgcaggt ctcggaggaa ttagtcatgg aggccaaggc tgcttttgag gatgctcagg
     1561 aggaagccag agcggagaag ctccgagaag cacttccacc attagtggca gacaaaggca
     1621 tcgaggcagc cgcagaagtt gtctgcgaag tggaggggct ccaggcggac atcggagcag
     1681 cattagttga aaccccgcgc ggtcacgtaa ggataatacc tcaagcaaat gaccgtatga
     1741 tcggacagta tatcgttgtc tcgccaaact ctgtgctgaa gaatgccaaa ctcgcaccag
     1801 cgcacccgct agcagatcag gttaagatca taacacactc cggaagatca ggaaggtacg
     1861 cggtcgaacc atacgacgct aaagtactga tgccagcagg aggtgccgta ccatggccag
     1921 aattcctagc actgagtgag agcgccacgt tagtgtacaa cgaaagagag tttgtgaacc
     1981 gcaaactata ccacattgcc atgcatggcc ccgccaagaa tacagaagag gagcagtaca
     2041 aggttacaaa ggcagagctt gcagaaacag agtacgtgtt tgacgtggac aagaagcgtt
     2101 gcgttaagaa ggaagaagcc tcaggtctgg tcctctcggg agaactgacc aaccctccct
     2161 atcatgagct agctctggag ggactgaaga cccgacctgc ggtcccgtac aaggtcgaaa
     2221 caataggagt gataggcaca ccggggtcgg gcaagtcagc tattatcaag tcaactgtca
     2281 cggcacgaga tcttgttacc agcggaaaga aagaaaattg tcgcgaaatt gaggccgacg
     2341 tgctaagact gaggggtatg cagattacgt cgaagacagt agattcggtt atgctcaacg
     2401 gatgccacaa agccgtagaa gtgctgtacg ttgacgaagc gttcgcgtgc cacgcaggag
     2461 cactacttgc cttgattgct atcgtcaggc cccgcaagaa ggtagtacta tgcggagacc
     2521 ccatgcaatg cggattcttc aacatgatgc aactaaaggt acatttcaat caccctgaaa
     2581 aagacatatg caccaagaca ttctacaagt atatctcccg gcgttgcaca cagccagtta
     2641 cagctattgt atcgacactg cattacgatg gaaagatgaa aaccacgaac ccgtgcaaga
     2701 agaacattga aatcgatatt acaggggcca caaagccgaa gccaggggat atcatcctga
     2761 catgtttccg cgggtgggtt aagcaattgc aaatcgacta tcccggacat gaagtaatga
     2821 cagccgcggc ctcacaaggg ctaaccagaa aaggagtgta tgccgtccgg caaaaagtca
     2881 atgaaaaccc actgtacgcg atcacatcag agcatgtgaa cgtgttgctc acccgcactg
     2941 aggacaggct agtgtggaaa accttgcagg gcgacccatg gattaagcag cccactaaca
     3001 tacctaaagg aaactttcag gctactatag aggactggga agctgaacac aagggaataa
     3061 ttgctgcaat aaacagcccc actccccgtg ccaatccgtt cagctgcaag accaacgttt
     3121 gctgggcgaa agcattggaa ccgatactag ccacggccgg tatcgtactt accggttgcc
     3181 agtggagcga actgttccca cagtttgcgg atgacaaacc acattcggcc atttacgcct
     3241 tagacgtaat ttgcattaag tttttcggca tggacttgac aagcggactg ttttctaaac
     3301 agagcatccc actaacgtac catcccgccg attcagcgag gccggtagct cattgggaca
     3361 acagcccagg aacccgcaag tatgggtacg atcacgccat tgccgccgaa ctctcccgta
     3421 gatttccggt gttccagcta gctgggaagg gcacacaact tgatttgcag acggggagaa
     3481 ccagagttat ctctgcacag cataacctgg tcccggtgaa ccgcaatctt cctcacgcct
     3541 tagtccccga gtacaaggag aagcaacccg gcccggtcaa aaaattcttg aaccagttca
     3601 aacaccactc agtacttgtg gtatcagagg aaaaaattga agctccccgt aagagaatcg
     3661 aatggatcgc cccgattggc atagccggtg cagataagaa ctacaacctg gctttcgggt
     3721 ttccgccgca ggcacggtac gacctggtgt tcatcaacat tggaactaaa tacagaaacc
     3781 accactttca gcagtgcgaa gaccatgcgg cgaccttaaa aaccctttcg cgttcggccc
     3841 tgaattgcct taacccagga ggcaccctcg tggtgaagtc ctatggctac gccgaccgca
     3901 acagtgagga cgtagtcacc gctcttgcca gaaagtttgt cagggtgtct gcagcgagac
     3961 cagattgtgt ctcaagcaat acagaaatgt acctgatttt ccgacaacta gacaacagcc
     4021 gtacacggca attcaccccg caccatctga attgcgtgat ttcgtccgtg tatgagggta
     4081 caagagatgg agttggagcc gcgccgtcat accgcaccaa aagggagaat attgctgact
     4141 gtcaagagga agcagttgtc aacgcagcca atccgctggg tagaccaggc gaaggagtct
     4201 gccgtgccat ctataaacgt tggccgacca gttttaccga ttcagccacg gagacaggca
     4261 ccgcaagaat gactgtgtgc ctaggaaaga aagtgatcca cgcggtcggc cctgatttcc
     4321 ggaagcaccc agaagcagaa gccttgaaat tgctacaaaa cgcctaccat gcagtggcag
     4381 acttagtaaa tgaacataac atcaagtctg tcgccattcc actgctatct acaggcattt
     4441 acgcagccgg aaaagaccgc cttgaagtat cacttaactg cttgacaacc gcgctagaca
     4501 gaactgacgc ggacgtaacc atctattgcc tggataagaa gtggaaggaa agaatcgacg
     4561 cggcactcca acttaaggag tctgtaacag agctgaagga tgaagatatg gagatcgacg
     4621 atgagttagt atggattcat ccagacagtt gcttgaaggg aagaaaggga ttcagtacta
     4681 caaaaggaaa attgtattcg tacttcgaag gcaccaaatt ccatcaagca gcaaaagaca
     4741 tggcggagat aaaggtcctg ttccctaatg accaggaaag taatgaacaa ctgtgtgcct
     4801 acatattggg tgagaccatg gaagcaatcc gcgaaaagtg cccggtcgac cataacccgt
     4861 cgtctagccc gcccaaaacg ttgccgtgcc tttgcatgta tgccatgacg ccagaaaggg
     4921 tccacagact tagaagcaat aacgtcaaag aagttacagt atgctcctcc accccccttc
     4981 ctaagcacaa aattaagaat gttcagaagg ttcagtgcac gaaagtagtc ctgtttaatc
     5041 cgcacactcc cgcattcgtt cccgcccgta agtacataga agtgccagaa cagcctaccg
     5101 ctcctcctgc acaggccgag gaggcccccg aagttgtagc gacaccgtca ccatctacag
     5161 ctgataacac ctcgcttgat gtcacagaca tctcactgga tatggatgac agtagcgaag
     5221 gctcactttt ttcgagcttt agcggatcgg acaactctat tactagtatg gacagttggt
     5281 cgtcaggacc tagttcacta gagatagtag accgaaggca ggtggtggtg gctgacgttc
     5341 atgccgtcca agagcctgcc cctattccac cgccaaggct aaagaagatg gcccgcctgg
     5401 cagcggcaag aaaagagccc actccaccgg caagcaatag ctctgagtcc ctccacctct
     5461 cttttggtgg ggtatccatg tccctcggat caattttcga cggagagacg gcccgccagg
     5521 cagcggtaca acccctggca acaggcccca cggatgtgcc tatgtctttc ggatcgtttt
     5581 ccgacggaga gattgatgag ctgagccgca gagtaactga gtccgaaccc gtcctgtttg
     5641 gatcatttga accgggcgaa gtgaactcaa ttatatcgtc ccgatcagcc gtatcttttc
     5701 cactacgcaa gcagagacgt agacgcagga gcaggaggac tgaatactga ctaaccgggg
     5761 taggtgggta catattttcg acggacacag gccctgggca cttgcaaaag aagtccgttc
     5821 tgcagaacca gcttacagaa ccgaccttgg agcgcaatgt cctggaaaga attcatgccc
     5881 cggtgctcga cacgtcgaaa gaggaacaac tcaaactcag gtaccagatg atgcccaccg
     5941 aagccaacaa aagtaggtac cagtctcgta aagtagaaaa tcagaaagcc ataaccactg
     6001 agcgactact gtcaggacta cgactgtata actctgccac agatcagcca gaatgctata
     6061 agatcaccta tccgaaacca ttgtactcca gtagcgtacc ggcgaactac tccgatccac
     6121 agttcgctgt agctgtctgt aacaactatc tgcatgagaa ctatccgaca gtagcatctt
     6181 atcagattac tgacgagtac gatgcttact tggatatggt agacgggaca gtcgcctgcc
     6241 tggatactgc aaccttctgc cccgctaagc ttagaagtta cccgaaaaaa catgagtata
     6301 gagccccgaa tatccgcagt gcggttccat cagcgatgca gaacacgcta caaaatgtgc
     6361 tcattgccgc aactaaaaga aattgcaacg tcacgcagat gcgtgaactg ccaacactgg
     6421 actcagcgac attcaatgtc gaatgctttc gaaaatatgc atgtaatgac gagtattggg
     6481 aggagttcgc tcggaagcca attaggatta ccactgagtt tgtcaccgca tatgtagcta
     6541 gactgaaagg ccctaaggcc gccgcactat ttgcaaagac gtataatttg gtcccattgc
     6601 aagaagtgcc tatggataga ttcgtcatgg acatgaaaag agacgtgaaa gttacaccag
     6661 gcacgaaaca cacagaagaa agaccgaaag tacaagtgat acaagccgca gaacccctgg
     6721 cgactgctta cttatgcggg attcaccggg aattagtgcg taggcttacg gccgtcttgc
     6781 ttccaaacat tcacacgctt tttgacatgt cggcggagga ttttgatgca atcatagcag
     6841 aacacttcaa gcaaggcgac ccggtactgg agacggatat cgcatcattc gacaaaagcc
     6901 aagacgacgc tatggcgtta accggtctga tgatcttgga ggacctgggt gtggatcaac
     6961 cactactcga cttgatcgag tgcgcctttg gagaaatatc atccacccat ctacctacgg
     7021 gtactcgttt taaattcggg gcgatgatga aatccggaat gttcctcaca ctttttgtca
     7081 acacagtttt gaatgtcgtt atcgccagca gagtactaga agagcggctt aaaacgtcca
     7141 gatgtgcagc gttcattggc gacgacaaca tcatacatgg agtagtatct gacaaagaaa
     7201 tggctgagag gtgcgccacc tggctcaaca tggaggttaa gatcatcgac gcagtcatcg
     7261 gtgagagacc accttacttc tgcggcggat ttatcttgca agattcggtt acttccacag
     7321 cgtgccgcgt ggcggatccc ctgaaaaggc tgtttaagtt gggtaaaccg ctcccagccg
     7381 acgacgagca agacgaagac agaagacgcg ctctgctaga tgaaacaaag gcgtggttta
     7441 gagtaggtat aacaggcact ttagcagtgg ccgtgacgac ccggtatgag gtagacaata
     7501 ttacacctgt cctactggca ttgagaactt ttgcccagag caaaagagca ttccaagcca
     7561 tcagagggga aattaaagca tctctacggt ggtcctaaat agtcagcata gtacatttca
     7621 tctgactaat accacaacac caccacctct agagccacca tggcacttcc cagatgcatg
     7681 tggccaaatt atgtttggag agctatgatg gcatgtgtgg tccacagggg atccggtgcc
     7741 ccattgactc tctgcttgtt gggatgtttg ctacagactt ttcacgtact ctctcaaaag
     7801 tatccatacg acgttccgga ctacgcagaa ttcatggaca aagactgcga aatgaagcgc
     7861 accaccctgg atagccctct gggcaagctg gaactgtctg ggtgcgaaca gggcctgcac
     7921 cgtatcatct tcctgggcaa aggaacatct gccgccgacg ccgtggaagt gcctgcccca
     7981 gccgccgtgc tgggcggacc agagccactg atgcaggcca ccgcctggct caacgcctac
     8041 tttcaccagc ctgaggccat cgaggagttc cctgtgccag ccctgcacca cccagtgttc
     8101 cagcaggaga gctttacccg ccaggtgctg tggaaactgc tgaaagtggt gaagttcgga
     8161 gaggtcatca gctacagcca cctggccgcc ctggccggca atcccgccgc caccgccgcc
     8221 gtgaaaaccg ccctgagcgg aaatcccgtg cccattctga tcccctgcca ccgggtggtg
     8281 cagggcgacc tggacgtggg gggctacgag ggcgggctcg ccgtgaaaga gtggctgctg
     8341 gcccacgagg gccacagact gggcaagcct gggctgggtg tcgacttgga tgatgtagac
     8401 ccattggtta ctactaactt tggcaagatt aggggaatta agaaagaact caataatgaa
     8461 attttgggtc ctgtcattca gtttcttggg gttccatatg ccgctccacc aacaggagaa
     8521 catcgtttcc agcctccaga accaccatct ccctggtctg acatccggaa cgccactcag
     8581 tttgctcctg tatgtcccca gaatatcatt gatggcagat tgcctgaagt tatgcttcct
     8641 gtgtggttca ctaataactt ggatgtggtt tcatcatacg tccaagacca gagtgaagac
     8701 tgtctatact taaacatcta tgtcccaact gaagatggtc cccttacaaa gaaacacaca
     8761 gatgatttag gtgataatga cggtgctgaa gatgaagata ttcgggacag tgggggtccc
     8821 aaaccagtga tggtgtacat ccatggcggc tcttacatgg aaggtactgg aaatctgtat
     8881 gatgggagtg tcttggcaag ctatggcaat gtgatcgtca tcacagtcaa ctatcggctt
     8941 ggggtacttg gcttcttgag cacaggggat caggctgcca aaggaaacta cgggctcctt
     9001 gacctcatcc aggccctaag atggaccagc gagaacattg ggttctttgg tggtgacccc
     9061 ttgcgaatca ctgtgtttgg atcaggcgct gggggttcat gtgtcaacct gctgacttta
     9121 tcccattatt ctgaaggtaa ccgttggagc aattcaacca aaggactttt tcaacgagca
     9181 atagctcaga gtggaacagc cctttccagc tgggctgtta gtttccagcc tgcaaaatac
     9241 gctagaattc tggccacaaa agttggctgc aatgtttcag atacagtaga gttagtagaa
     9301 tgcctgcaga agaagcctta caaagaactt gttgatcaag atgttcaacc agcccgatac
     9361 cacatagcct ttggacctgt gatcgatggt gatgtaatac cagatgaccc tcagatactg
     9421 atggaacaag gagagttcct caactatgat ataatgttgg gagttaacca aggggaaggg
     9481 ttgaagtttg tcgaaaatat agtagatagt gatgatggtg tatcagccag tgatttcgac
     9541 tttgctgttt ctaattttgt tgataattta tatggatatc ctgaaggcaa agatgttttg
     9601 agagaaacca ttaaattcat gtatactgac tgggctgatc gccataatcc tgaaactaga
     9661 aggaagacat tgttggcttt gtttacggac catcaatggg tagcacctgc tgtggccaca
     9721 gcagaccttc actcgaactt tggctcacct acatacttct atgcctttta tcatcattgc
     9781 caaacagatc aagttccagc ttgggctgat gcagctcatg gggatgaggt tccctatgtg
     9841 ttgggaatcc ccatgattgg ccctacagag ttatttcctt gcaatttctc caagaatgat
     9901 gtgatgttga gtgcagtagt aatgacatac tggacgaatt ttgctaaaac tggtgaccca
     9961 aatcaaccag ttcctcaaga cacaaaattc atccatacca aacccaaccg ctttgaagaa
    10021 gtagcatgga ccagatattc ccagaaagac cagctttatc tccatattgg attaaaaccg
    10081 agagttaaag agcattacag agccaataag gtaaatctct ggctggagct ggtacctcat
    10141 ctgcataatc tcaatgacat ttctcagtat acctcgacaa caactaaagt gccatccacg
    10201 gacatcactc tcagacctac aaggaagaat tccacaccag tcacatcagc ctttcccact
    10261 gccaaacagg atgatcccaa gcaacaacca agccccttct cggtggatca gagggactac
    10321 tccacagagc taagtgtcac tatcgcagtg ggggcctctc tgctgtttct caacatcttg
    10381 gcttttgcag ccctgtacta caagaaggat aagaggagac atgatgtcca ccggaggtgc
    10441 agccctcagc gcacgaccac caacgaccta acccatgctc cagaagagga aattatgtct
    10501 ctccaaatga agcacactga cttggatcac gagtgtgagt ccatccatcc acatgaggtg
    10561 gttcttcgga ccagcgctcc accgctcgac ggagccggag ctggcgctgg agctggagcc
    10621 ggagctggcg ggctagccac catggacgca caaacacgac gacgtgagcg tcgcgctgag
    10681 aaacaagctc aatggaaagc tgcaaacctc gagccaccgc tcgacggagc cggagctggc
    10741 gctggagctg gagccggagc tggcggtcta gccaccccac cgagcgctgc ctgtccccca
    10801 gattatactc tagctatgag gaggtcacct gatgatattc cactaatgac acctaacacc
    10861 atcacaatga ttcccaacac tataccaggg attcagccct tacatacatt caacacattt
    10921 actggaggac agaataatac actgccccat ccccacccac acccccattc acattcaaca
    10981 accagggtat aggcatgcag gccttaaagc atctctacgg tggtcctaaa tagtcagcat
    11041 agtacatttc atctgactaa taccacaaca ccaccacctc tagtagaggc gcgccaccat
    11101 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
    11161 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
    11221 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
    11281 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
    11341 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
    11401 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
    11461 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
    11521 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
    11581 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
    11641 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
    11701 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
    11761 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtctt aggggcccaa
    11821 tgatccgacc agcaaaactc gatgtacttc cgaggaactg atgtgcataa tgcatcaggc
    11881 tggtacatta gatccccgct taccgcgggc aatatagcaa cactaaaaac tcgatgtact
    11941 tccgaggaag cgcagtgcat aatgttgcgc agtgttgcca cataaccact atattaacca
    12001 tttatttagc ggacgccaaa aactcaatgt atttttgagg aagcgtggtg cataatgcca
    12061 cgcaggtttt gcataatttt tttttttttt tttattaatc aacaaaattt tgtttttaac
    12121 atttcaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag gggaattcct cgataattaa
    12181 gcggccgctt aattaatcga ggggaattaa ttcttgaaga cgaaagggcc aggtggcact
    12241 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
    12301 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
    12361 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
    12421 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
    12481 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
    12541 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
    12601 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
    12661 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
    12721 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
    12781 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
    12841 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
    12901 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
    12961 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
    13021 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
    13081 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
    13141 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
    13201 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
    13261 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
    13321 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
    13381 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
    13441 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
    13501 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
    13561 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
    13621 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
    13681 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
    13741 gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg
    13801 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
    13861 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
    13921 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
    13981 aacgccagca acgcgagctc gtatggacat attgtcgtta gaacgcggct acaattaata
    14041 cataacctta tgtatcatac acatacgatt taggggacac tatag
//
