LOCUS       sequence-169802-       11755 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for Gal4 inducible humanIL-2 with mCherry reporter
            and constitutive tBFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11755)
  AUTHORS   Roybal KT, Williams JZ, Morsut L, Rupp LJ, Kolinko I, Choe JH, 
            Walker WJ, McNally KA, Lim WA
  TITLE     Engineering T Cells with Customized Therapeutic Response Programs 
            Using Synthetic Notch Receptors.
  JOURNAL   Cell. 2016 Oct 6;167(2):419-432.e16. doi: 
            10.1016/j.cell.2016.09.011. Epub 2016 Sep 29.
  PUBMED    27693353
REFERENCE   2  (bases 1 to 11755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.09.011"; journalName: "Cell"; date: "2016-10-6-
            6"; volume: "167"; issue: "2"; pages: "419-432.e16"
COMMENT     Sequence Label: pHR_Gal4UAS_humanIL-2_IRES_mCherry_PGK_tBFP
FEATURES             Location/Qualifiers
     source          1..11755
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     166..190
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     sig_peptide     280..339
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     misc_feature    760..1333
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(927..944)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1154..1173
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             1352..2062
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1497..1515)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1765..1784
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        2147..2634
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2182..2205)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2567..2586
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2655..3356
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    3392..3980
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3445..3465)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3863..3874)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3983..3999)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3984..4000)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4090..4323
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4348..4366)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4349..4366)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4656..4675)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4889..4908)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5008..5030
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5068..5086)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5154..5258
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5259..5327,5328..6119)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5477..5496)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6290..6878
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6779..6798
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7032..7049
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7119..7139)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7124..7453
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7304..7439
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8587..8652
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8782..8802
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9227..9361
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9264..9283)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9318..9337
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9529..10162
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10209..10334
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10831..11064
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11249..11293
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11578..11695
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggagcactgt cctccgaacg tcggagcact gtcctccgaa cgtcggagca ctgtcctccg
       61 aacgtcggag cactgtcctc cgaacggagc atgtcctccg aacgtcggag cactgtcctc
      121 cgaacgacta gttaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa
      181 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
      241 ccgatccagc ctctcgacat tcgttggatc gccgccacca tgtaccgaat gcagctcctg
      301 tcttgtattg ctctctcttt ggccctcgtt accaatagtg cccctacctc cagttccacg
      361 aaaaagaccc agctccagct tgagcacctg ctgctggatc tgcaaatgat cttgaacggc
      421 ataaataact acaagaatcc caagctgacg cgaatgctga ccttcaagtt ctacatgcct
      481 aagaaggcca ctgagctcaa acatcttcag tgcctggagg aagagcttaa gccattggaa
      541 gaggtgttga atctcgccca atctaagaac tttcatctgc ggccaaggga tcttatctcc
      601 aatatcaacg ttatcgttct ggaactgaaa ggatccgaga caaccttcat gtgcgagtac
      661 gccgatgaaa cggccaccat tgtggagttc ttgaaccggt ggattacgtt ttgtcagtca
      721 ataatatcta ctctgacgta agatccttga cttgcggccc ccctctccct cccccccccc
      781 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
      841 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
      901 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
      961 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     1021 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     1081 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     1141 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     1201 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     1261 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     1321 aacacgatga taaatgagcg ctggcgggtc catggtgagc aagggcgagg aggataacat
     1381 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     1441 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     1501 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     1561 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     1621 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     1681 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     1741 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     1801 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     1861 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     1921 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     1981 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     2041 catggacgag ctgtacaagt agggccgcaa ctcccacctg caacatgcgt gactgactga
     2101 ggccgcgact ctagagtcga cctgcacgag gttaacgaat tctaccgggt aggggaggcg
     2161 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac
     2221 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg
     2281 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc
     2341 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc
     2401 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa
     2461 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc
     2521 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
     2581 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
     2641 agcccaagct taccatgagc gagctgatta aggagaacat gcacatgaag ctgtacatgg
     2701 agggcaccgt ggacaaccat cacttcaagt gcacatccga gggcgaaggc aagccctacg
     2761 agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg
     2821 acatcctggc tactagcttc ctctacggca gcaagacctt catcaaccac acccagggca
     2881 tccccgactt cttcaagcag tccttccctg agggcttcac atgggagaga gtcaccacat
     2941 acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca
     3001 tctacaacgt caagatcaga ggggtgaact tcacatccaa cggccctgtg atgcagaaga
     3061 aaacactcgg ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag
     3121 gcagaaacga catggccctg aagctcgtgg gcgggagcca tctgatcgca aacatcaaga
     3181 ccacatatag atccaagaaa cccgctaaga acctcaagat gcctggcgtc tactatgtgg
     3241 actacagact ggaaagaatc aaggaggcca acaacgagac ctacgtcgag cagcacgagg
     3301 tggcagtggc cagatactgc gacctcccta gcaaactggg gcacaagctt aattaatgca
     3361 ggcatgcaag cttgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
     3421 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     3481 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     3541 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     3601 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     3661 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     3721 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     3781 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     3841 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     3901 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     3961 cctttgggcc gcctccccgc atcgataccg tcgacctcga gggaattaat tcgagctcgg
     4021 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     4081 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     4141 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     4201 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     4261 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     4321 gcagcatcta gaattaattc cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat
     4381 acataaggtt atgtattaat tgtagccgcg ttctaacgac aatatgtaca agcctaattg
     4441 tgtagcatct ggcttactga agcagaccct atcatctctc tcgtaaactg ccgtcagagt
     4501 cggtttggtt ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt
     4561 cagcgaacca atcagcaggg tcatcgctag ccagatcctc tacgccggac gcatcgtggc
     4621 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
     4681 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
     4741 aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc
     4801 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa
     4861 gggagagcgt cgaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     4921 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     4981 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     5041 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     5101 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     5161 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5221 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5281 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5341 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5401 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5461 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5521 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5581 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5641 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5701 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5761 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5821 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5881 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5941 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6001 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6061 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6121 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6181 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6241 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6301 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6361 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6421 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6481 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6541 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6601 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6661 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6721 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6781 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6841 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6901 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6961 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7021 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     7081 ctctccccgc gcgttggccg attcattaat gcagctgtgg aatgtgtgtc agttagggtg
     7141 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     7201 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     7261 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     7321 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     7381 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     7441 aggcttttgc aaaaagcttg gacacaagac aggcttgcga gatatgtttg agaataccac
     7501 tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg
     7561 tttttagtgc gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt
     7621 aagccgtaga taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg
     7681 acatgttgca gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga
     7741 aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact
     7801 gggtattcgt catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga
     7861 gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct
     7921 ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt
     7981 caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatatccc
     8041 gcaagatacc tggattgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc
     8101 cggtcgctaa tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggcg
     8161 ggttacaata gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga
     8221 gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct
     8281 ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc atccctcact
     8341 ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc
     8401 tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg taccgacgat gatttatacg
     8461 atacggtgat tggctaccgt ggcggcaact ggatttatga gtgggccccg gatctttgtg
     8521 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
     8581 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
     8641 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt
     8701 aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct
     8761 gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt
     8821 ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc
     8881 tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa
     8941 tattctgtaa cctttataag taggcataac agttataatc ataacatact gttttttctt
     9001 actccacaca ggcatagagt gtctgctatt aataactatg ctcaaaaatt gtgtaccttt
     9061 agctttttaa tttgtaaagg ggttaataag gaatatttga tgtatagtgc cttgactaga
     9121 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     9181 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     9241 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     9301 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     9361 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
     9421 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc
     9481 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa
     9541 ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     9601 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat
     9661 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga
     9721 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccgtag agagaagtgt
     9781 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg
     9841 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc
     9901 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag
     9961 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
    10021 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
    10081 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
    10141 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
    10201 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
    10261 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
    10321 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
    10381 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
    10441 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
    10501 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
    10561 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
    10621 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc
    10681 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
    10741 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
    10801 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
    10861 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
    10921 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
    10981 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
    11041 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
    11101 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
    11161 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
    11221 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
    11281 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
    11341 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
    11401 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
    11461 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
    11521 cattcgatta gtgaacggat ctcgacggta tcgccaaatg gcagtattca tccacaattt
    11581 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
    11641 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
    11701 ttattacagg gacagcagag atccagtttg gatcgataag cttgatatcg aattc
//
