LOCUS       sequence-169804-       10128 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of SZT2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10128)
  AUTHORS   Wolfson RL, Chantranupong L, Wyant GA, Gu X, Orozco JM, Shen K, 
            Condon KJ, Petri S, Kedir J, Scaria SM, Abu-Remaileh M, Frankel WN, 
            Sabatini DM
  TITLE     KICSTOR recruits GATOR1 to the lysosome and is necessary for 
            nutrients to regulate mTORC1.
  JOURNAL   Nature. 2017 Feb 15. doi: 10.1038/nature21423.
  PUBMED    28199306
REFERENCE   2  (bases 1 to 10128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2017 Feb 15. doi: 10.1038/nature21423."
COMMENT     Sequence Label: pRK5-HA-SZT2
FEATURES             Location/Qualifiers
     source          1..10128
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggcctcgg agcgcccgga gccggaggtg gaagaagctg ggcaggtgtt cctgttaatg
       61 aaaaaggatt atcgaatctc ccgaaatgtt cgcctggctt ggttcctcag tcatctgcac
      121 caaactgtgc aggccacacc ccaggagatg ctgcttcagt ctgaacagga attggaagtc
      181 ctcagtgtcc tgccccctgg gtggcagcca gatgaaccag tggtcccaag gccattcctc
      241 ctggtacctt ccacccgggt caccttcctg gcttggcagt atcggtttgt cattgagttg
      301 gaccttagcc catctactgg cattgtggat gattccacag gggagatctt gtttgatgaa
      361 gttttccatg ccctgtcccg ctgcttaggc gggctgcttc ggcccttccg agtgcctgga
      421 tcttgcatcg acttccagcc tgagatctat gtaactatcc aggcctactc ctccatcatt
      481 ggactgcagt cccaccaggt gctggtacag ggctgcctct tggacccttc ccagcgggag
      541 gtgttcctgc agcagatata tgagcagctc tgcctctttg aggataaggt ggccaccatg
      601 ctgcagcagc agtacgatcc ccagagccag gcagaagacc agtccccaga ctcaggggac
      661 ctactgggcc ggaaggtagg cgtctccatg gtgacagctg atcttgggct ggtcagtatg
      721 attcgtcagg gcatcttggc actgcagtta ctaccctcga actctagtgc agggattatc
      781 gtgatcacgg atggggtgac cagtgtacct gatgttgctg tctgtgagac actgctgaac
      841 cagcttcgca gtggcactgt ggcttgttcc tttgtccagg tgggaggagt ttactcttat
      901 gactgcagtt ttggccatgt gcccaatgtg gaattaatga agttcatcgc aatggcaaca
      961 tttgggtcct acctgtccac ttgtcctgag ccggagccag gcaacctggg tctgactgtc
     1021 taccaccggg catttctcct ctattccttc ctgcgcagtg gggaagcact gaaccctgaa
     1081 tattactgcg gctctcagca ccgcctattt aatgagcacc tggtctctgc aagcagcaac
     1141 cctgccctgg ccttgcgccg gaagaagcac actgagaagg aggtgccagc cgacttggtc
     1201 agcactgtgt ccgtacggct tcgagagggc tacagtgtcc gagaggtcac actggccaaa
     1261 ggagggtccc aattggaggt aaagctggtg ctgctgtgga aacacaacat gcgcattgag
     1321 tatgtggcta tggcaccctg gcccctggag cctgagggcc ctcgagtaac acgggtggaa
     1381 gtgacgatgg aaggcggcta cgacattttg catgatgtgt cctgtgcact aaggcagccc
     1441 attcgttcat tgtatcgtac ccatgttatc cggcgtttct ggaacacgct gcagagcatc
     1501 aaccagacag accagatgct tgcccacctt cagtccttct cctcagtgcc tgagcatttc
     1561 acgcttcctg acagcaccaa gagcggagtg ccactcttct acatccctcc aggctccacc
     1621 accccggtgc tctccctcca gcccagtggt tctgactcat cccatgccca gtttgctgcc
     1681 tactggaagc cagtgctgtc catggatgca aattcctggc agcgatggct gcacatgcat
     1741 cgcctggtgc taatcctgga gcatgacaca ccaatcccca agcacttgca caccccgggc
     1801 agcaatgggc gctacagcac tatccagtgc aggatctccc actcctccct gacctctctg
     1861 ctgcgggact ggagcagctt cgtactagtc gagggctatt cttatgttaa gctgctctcc
     1921 agtgccccag accagccccc caattccttc tacatggtcc gtatcatttc caaggcccca
     1981 tgcatggttc ttcgcctggg ttttcccatt ggcacaccag caccggcccg gcacaagatt
     2041 gtgtcaggct tgagggaaga gatcctgcgg ctgcgtttcc cccaccgggt acaaagcaag
     2101 gagccaacgc ccaaggtgaa acgaaaaggg ctagggggtg ctggtggggg cagctctccc
     2161 tccaagtcac cccccgtgct ggggccacag caggccctgt ctgaccggcc ctgccttgtg
     2221 gtcctgcata agccactgga caaactgctc atcaggtatg agaagctgcc cttggactac
     2281 cgggcaccct tcttgctgac attggagcca ccaggtccac tgcccttggt gtcaggccgc
     2341 tcagcctctt ctagcctggc gtcactgtcc cgctacctct accatcagcg ctggctttgg
     2401 agtgtcccgt caggactggc ccctgcgctg cctctcagtg ccattgccca gctcctctcc
     2461 atcctcactg aagtccgact ttctgaagga ttccacttcg cctgcagtgg ggaaggaatc
     2521 atcaacatgg ttctggagct tccaattcag aatgaaccac cagggcaggc tgcagctgaa
     2581 gagaagcaca cctgtgttgt ccagtacatc ctcttccccc cacactctac ctccaccaaa
     2641 gacagcttct cgacagatga tgacaatgat gtggaagtgg aggccctgga gggagactca
     2701 gagctcaatc tggtcactga ggtgtgggtg gagccacagt atgggcgagt gggacctggc
     2761 cctggaatct ggaagcacct ccaggacctg acgtattctg agatcccgca agctctccac
     2821 ccacgggatg ctgcctgcat aggctccatg ctgagctttg aatacctgat acagctgtgt
     2881 cagagcaagg aatggggtcc tctgccccca gagccgaggg tctctgatgg attggatcag
     2941 ggaggagaca cctgcgtcca tgagatccct ttccattttg acctaatggg attgctgcca
     3001 cagtgccagc agctccagat gttcttcctc ttgcttgcca gaggtgccga ggggccactg
     3061 ctgggggttc atgggatccc gaaggagcaa gcagtcggca gcacccaggc cacaggagac
     3121 tccgctttta cttccctgag tgtaggtctt cctgaaactc tcaagcctct catctctgcc
     3181 cagccccctc agtggcgctg ctatgcaagg cttgtgaacc cccagcatgt gtttctgact
     3241 tttctcccag ctaccttctc agatgtccag cgtctggctg cctgtggcct ggagggaccc
     3301 cctcaagagg agacaaagcc taagtttggg gattggagtg gggctcccag tctgaaagat
     3361 ctaggaggaa ctgggatcaa agctacaaag tcccacgtcc ctgtcctcag tgtgaccctg
     3421 gctagtgaca atgcccagaa tcaaggagag ctaagtccac cattccgtcg agacttacag
     3481 gcttacgctg ggcgtcaggc ttcccagaca gagagtgcgg atgggccccg gacccggtgt
     3541 cctgtctaca tctacagctg ttcactggaa gcgctgaggg aacaaatggt tggcatgcag
     3601 ccccctcagg cgccccgaga cctcatcttc cggactcagt tcctcgacca cccctcccca
     3661 tcctcagcct ggatggaacc ccggtacaag gaggcagcta accactgtgc cctgctgcag
     3721 gagcatgcac agcggtgcta tgtccgtggg ctattccgca gcttgcagca agcacagagt
     3781 gtgacctccc aggatttgct gacagcggta gatgcctgtg aggagctact acaagaaata
     3841 gacatcaccc catttctcct tgcattgtgt ggccacactt ggggtttgcc tcatgcaccc
     3901 ccaagtcctg gtcctctcag ccctgggccc ttcagcagca gcatggagga gggtgctgaa
     3961 cctcgggaac gagctatcct agcttctgaa tccagcatag agaccgagga cctaagcgag
     4021 cctgagtttc agagcacccg tgtccctggc attccagacc ctgggccaga gatctctctg
     4081 acagatgtct gccagctcag aggagaggcc catggtgccc ttcatagcgt catccaggag
     4141 aagttcctag agatcagtcg tctccacttc cgcacagtgc cttccaatcc ccactacttc
     4201 ttctattgcc ctccatccag caggcgagaa gatgaggggc ctcgggacac agtagacaga
     4261 aaaatcagtg acctggagtt ttcagaggct gagcttatgg gagaagaagg agacacatct
     4321 gcctgctgtg tggtcactga gagtgaccca gagctagagg tagaataccg ggagagccgt
     4381 gaatcagacc tggggcctgc tgggctagac tctgcctcgc tgtcagacgt agacactgtg
     4441 aatcctgatg aagactcctt cagtatcttg gggggcgact cacccactgg gcctgagagc
     4501 ttccttcatg acctgccacc gctcttcctg cacctcacgt gctccgtgcg gctacgtggg
     4561 cagcacagct cagtacctgt gtgcagcctg cctacctgcc tgggccaggt gctttccagt
     4621 ctggagggcc ccccagttgg aggccgagtt cccttgaggg acctcagtgt gactctggat
     4681 gtcttcatgc tgactttgcc cctggaagtg gagctcccca cggcctcgga ccctcagcac
     4741 caccggtcaa catctgaaag cagtgcttca tttccacgat ccccagggca gccatcatct
     4801 ttaaggtcag atgatggcct cgggccccca ctgccacccc cagaagagga gaggcaccca
     4861 ggactatcca atttggccac gccccacaga ctggctattg agaccaccat gaatgagatc
     4921 cgctggttgt tggaagatga gatggtgggg gcactccgaa gagggggcat cccacagagt
     4981 cctgccctgc accgcgcagc tgcccatatc catagttctc ctggacgctc cacctgcctt
     5041 cgccaaactc tgccactgag ttttgtattt gggccagagc gttccctcac acaattcaag
     5101 gaggagttcc gccgcctcca tctccctggc catgttcttc ttgaagaccc tgacagtggc
     5161 ttcttctttg tggcagctgg ccaacagcca ggtgggtccc atggggagcc ttcttcagcg
     5221 gcctgggctt ggcacagtca tgaggacagg gctgaaggca tcgaagggga gaccctgaca
     5281 gccagccccc aagcacctgg gtccccagag gattctgagg gtgtccccct catcagcctg
     5341 ccccgcgtgc cacagggagg gagtcagcct gggcccagcc ggggattaag tctcatgtcc
     5401 agtcagggca gtgtggactc agaccaccta ggttatgatg gtggcagcag tggctcagac
     5461 agtgagggtc ccaatgacac ccttggtgag aaggccccct tcacattgcg gactccacct
     5521 gggccagcac ctccacagcc ttcactctca ggcctccctg ggccctgcct gcctgacttc
     5581 tggctcattg tccgggtcct gcaggaccgt gtggaagtgt atgcacatgc acggagcctg
     5641 attcgggagg atggggggcc gggcactgag tgtcgccacc tgcagcagct cctggtgagg
     5701 cgagttgggg agatctgcag ggaggtcaac cagcgactgc ttcttcaaga ccttcatgac
     5761 agccacgtgt gtaactctct tctggtggcc gagagtgaag aagatctgtg gcgcagtgag
     5821 actcccttcc actcccgtca gcgggcacca ctgcccagtg atgattatgc tgctgatgag
     5881 agctgtgcgc cccgtgggta cctggcagcc acaatgcagt ttgtccctgg ccatttctcc
     5941 tgtgacgttg tgtggggaac tgtgatccga gtccattcac gcctcaaaat ggggcccagc
     6001 atgggggtct ctcgggccat ccaggccctg cgctctgtgc tcaatgcctt ctctgtggtg
     6061 aaccggaaaa acatgtttgt ttaccaggag cgagcaacaa aggctgtgta ctatcttcgg
     6121 ctcctagaga catcctgcag tgaccggcca tggaaagggg atgcgctgcc cccttccctc
     6181 gctctgtccc gaagccaaga gcccatctac tctgaggaag cctcgggtcc tcgttctccc
     6241 ttagacatgg tctctagccg cagttcagat gctgctcgtc ctgtgggcca agtggacaga
     6301 catatccagc tgctggtcca tggtgttggg caggcaggtc ctgagatcac ggatgagctc
     6361 gtgcgagttc tatgtcggcg cctggatgag gccacgctgg acgtcatcac agttatgctt
     6421 gttcggaact gcaagctgac accagctgat gtggagttca tccagccccc tggaagtctc
     6481 ccctcagagg tgctgcatct ggccctaccc acctcctgca ggccctggct tccagccctg
     6541 gcatggtacc tacggcagaa cttgctcatc ttcctgcact ctcccaagta cacagatagc
     6601 aacagccgga accacttcca acatccactc ccaccacagg gtggcctccc tgacttggac
     6661 atctacttgt ataacaagcc tggtggacag ggcactgggg gcaaaggggt tgcctgcatc
     6721 actctagcct ttgtggatga aggaggggcc cccttgtcac tggcgttgtg gcccccctcc
     6781 tctccggggc ccccagaccc actgcgagag gaggaatttg agcaactgac ccaggtcatc
     6841 cgctgcccgg ttgttgtgga cagttcttca gctcagaatg gggccccacg gcttcgattg
     6901 gatgtgtggg aaaaggggaa cattagtatt gtgcagctgg aggagaaact ccgaggagca
     6961 gctcgccagg ccctggccga tgccatcatc gagcttcagc tgctgccagc ttcactatgt
     7021 acagaggaca cacccacagg aagtctcagg aacggatcgt tggaaactaa gagctctgca
     7081 ggccgagcta gcacctttcc ccctgcccct gtccctgggg agcctgtgac tccacccagc
     7141 aaagcgggcc ggcgtagctt ctgggatatg ctgagtaaaa cagaatgtgg ggatttgggt
     7201 tcccccaaaa caactgatga cattgtcctg gatcggccag aagacactcg gggccggagg
     7261 cgtcacaaaa ccgagagtgt tcggactcct ggtggagctg agcgggcgcc aggctcagat
     7321 tctggagccc agagacaaaa gcgccggaca acacagctag aagagggtga ggtggggacc
     7381 cttcatcctg tgtttgcccg tgttgctcag cgctggatgg agtttatggt tcagattggt
     7441 tgtgcctcag tgtccagaag ctctgcccac atggtgtccc ggttcctcct tccatccatc
     7501 ctgtctgagt tcaccgcact ggtcacctca atggctggag acaccagtgt ccgcatcttt
     7561 gagcagcatt tgggttcaga gccagagatc ttcggccctt gttcccctgg gcaactgggc
     7621 ccctctcccc gccctgcagc tgagcggcat ctgctgcttc tgggaaggaa cttcttgcag
     7681 tggaggagac caacacagca ggctgccaaa gccatgcagc gcttcgagcc aggaggtgat
     7741 gggagctcag ggcgaaatgc tccccggcag aggctcttgc tactagaggt tgtggacaag
     7801 aagctacagc tgctgaccta caactgggct ccagacctgg gggcagcatt gggccgagcg
     7861 ctggttcgcc tggtgcagtg gcagaatgca cgagcccatc tcatcttctg cctactcagc
     7921 cagaagcttg gcctcttcca tcattatggc cagttggact tccccgtgcg agatgaaaag
     7981 gagccaaacc cattcctgct gccgaccatg gaagtggaga ccctcatccg gagtgcaagt
     8041 cccccgctga gccgtgagca gggccgactg agtgggtcct ctcgtggtgg gggtcctctt
     8101 cccctggaca cattcccctt tgacgaggcc ctaagggata tcacggctgc ccgccccagc
     8161 tccgtacttg gtcctgtgcc cagacctcct gatcctgtca cctaccatgg acaacagttc
     8221 ctagagatca agatggcaga gcgcagagag ctggagcgcc agatgaagat ggaaaacctg
     8281 tttgtaacct ggcagcagcg ttctacccca gccaccatgc ccatcagtgc tggagagctg
     8341 gagaccctga agcagtcatc ccgcctggtg cattactgtg caacagccat gctcttcgac
     8401 ccagctgcct ggctgcatgg gcccccagag acctctggac cccctgacgg gcagcggcgc
     8461 catcgccctg agtcagggtc tgggagccga gaggccccca caagctgtga atccttggat
     8521 gtgtcgcccc cgggagcccg tgaggagcct tggctgaagg agctgagctt ggctttcctg
     8581 cagcaatatg tgcagtatct gcagagcata ggttttgtgc tggtaccact gcggcccccc
     8641 tcacccgccc gcagcaccag ccggccacgg gccatggcta tccttggaac agagggtcga
     8701 ggctccttct cctgccctaa aaccaagact gatgggagcc ccaagagcac tagctctccg
     8761 gtaaccacct accacctgca gcgggcactg cctgggggca tcatcctcat ggaactggca
     8821 ttccagggct gttacttctg tgtcaaacag tttgccctgg aatgttcccg aatcccaatg
     8881 gggcaggctg tcaactcaca gctgtccatg ctgttcacag aggagtgtga caaggtgcgg
     8941 gacctgatgc acgtgcactc gttcagctat gacttccatc tgcgcctcgt gcatcagcac
     9001 gtgctaggtg cccatctggt gctgcggcac ggctaccacc tcaccacctt tctgcgacac
     9061 ttcctggccc accaccctga cggaccccac tttggccgca atcacattta ccaagggaca
     9121 ttggagctcc ccacaccact cattgctgcc caccagctat acaactacgt ggctgatcac
     9181 gccagctctt accacatgaa gccattgcga atggcccggc cagggggccc agaacacaac
     9241 gagtatgccc tggtgtcggc atggcacagt tctggctcct acctggactc tgagggactt
     9301 cgacaccagg atgactttga tgtgtctctg cttgtctgtc actgtgctgc accctttgag
     9361 gagcaaggag aggctgagcg gcacgttctg cggctacagt tcttcgtggt gctcaccagc
     9421 cagcgagagc tcttccccag gctcactgct gacatgcgcc gcttccggaa gccacccaga
     9481 ctgccccctg agccagaggc tcctgggagt tcagctggca gccctgggga ggcctcaggg
     9541 cttattctag cgcctggacc ggctcctctg ttcccaccac tggctgcaga ggtgggcatg
     9601 gcacgagcac ggctggctca gctggtgcgg ctggctggag ggcactgccg tcgggacacc
     9661 ctttggaagc gcctcttctt gctggagcca ccggggcctg atcgactgcg gctagggggg
     9721 cgcctggccc tggcagagct ggaggaactc ctagaagcag tccatgccaa atccattggg
     9781 gacatcgacc cccagctgga ctgcttccta tccatgacgg tctcctggta ccagagcctg
     9841 atcaaagttc tcctaagccg cttcccccag agctgtcgcc atttccaaag cccagacttg
     9901 ggaactcagt acctggttgt gctgaatcag aagttcactg actgctttgt gctagtgttt
     9961 ctggactccc acttaggaaa gacgtctctg acagtggttt tccgagagcc cttcccagta
    10021 cagccccagg acagcgagag cccccctgcc caactggtct ccacctacca ccacctggag
    10081 tctgtcatca acacagcctg tttcaccctc tggacccgcc tcctctga
//
