LOCUS       sequence-169825-       11448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses NLS-tdiRFP-2A-LBR in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11448)
  AUTHORS   Seirin-Lee S, Osakada F, Takeda J, Tashiro S, Kobayashi R, Yamamoto 
            T, Ochiai H
  TITLE     Role of dynamic nuclear deformation on genomic architecture 
            reorganization.
  JOURNAL   PLoS Comput Biol. 2019 Sep 11;15(9):e1007289. doi: 
            10.1371/journal.pcbi.1007289. eCollection 2019 Sep.
  PUBMED    31509522
REFERENCE   2  (bases 1 to 11448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Comput
            Biol."; date: "2019-09-11"; volume: "15(9):e1007289. doi"; pages: " 
            10.1371/journal.pcbi.1007289. eCollection 2019 Sep"
COMMENT     Sequence Label: pLR5-CAG-NLS-tdiRFP670-2A-LBR
FEATURES             Location/Qualifiers
     source          1..11448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(89..544)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(226..247)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     438..457
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     671..693
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     685..702
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     686..702
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        712..730
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   760..794
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1117..1496
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1498..1774
                     /label=chicken beta-actin promoter
     intron          1775..2791
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2715..2737
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2799..2818
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        2889..2907
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2987..2996
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2999..3019
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4979..5032
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     intron          6982..7211
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     7194..7213
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(7284..7858,7859..7861,7862..7870)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7451..7468)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7678..7697
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7893..8696
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7956..7975)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8566..8585
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8826..8947
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8863..8882)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   9234..9296
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9347..9367)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9347..9365)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9386..9402)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9386..9402)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9399..9421)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9410..9426
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9434..9440,9441..9458,9459..9464))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9479..9500
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9617..9634)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9788..10376)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9868..9887)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10547..11338,11339..11407))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11170..11189
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(11408..11448,1..64))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
       61 cgcgcacatt tcccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc
      121 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
      181 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
      241 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
      301 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
      361 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
      421 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
      481 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
      541 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
      601 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
      661 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac
      721 tcactatagg gcgaattggg gcgcgccatt ctagattaac cctagaaaga tagtctgcgt
      781 aaaattgacg catgcattct tgaaatattg ctctctcttt ctaaatagcg cgaatccgtc
      841 gctgtgcatt taggacatct cagtcgccgc ttggagctcc cgtgaggcgt gcttgtcaat
      901 gcggtaagtg tcactgattt tgaactataa cgaccgcgtg agtcaaaatg acgcatgatt
      961 atcttttacg tgacttttaa gatttaactc atacgataat tatattgtta tttcatgttc
     1021 tacttacgtg ataacttatt atatatatat tttcttgtta tagatatcaa ctagaatgct
     1081 agctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
     1141 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     1201 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     1261 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     1321 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     1381 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     1441 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg
     1501 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt
     1561 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggncg
     1621 cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc
     1681 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
     1741 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc
     1801 cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact
     1861 cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta
     1921 atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc
     1981 tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc
     2041 gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc
     2101 gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg
     2161 ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt
     2221 gggcgcgtcg gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg
     2281 cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg
     2341 ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc
     2401 gggggagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag
     2461 ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg
     2521 tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg
     2581 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg
     2641 tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg
     2701 acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa
     2761 ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct
     2821 gtctcatcat tttggcaaag agctctctgg ctaactagag aacccactgc ttactggctt
     2881 atcgaaatta atacgactca ctatagggag acccaagctt ggtatcgacc tcggatcgtc
     2941 gctagcgata tcggcgcgcc agcatttaaa tctgtacaga ccggtcgcca ccatgggccc
     3001 aaaaaagaaa agaaaagttg aattcatggc cagaaaagtg gacctgacca gttgtgaccg
     3061 agagccgata cacattcctg gctctatcca gccatgtggg tgtttgctgg cctgcgatgc
     3121 ccaggcagtc aggattactc gaattaccga aaatgcgggt gccttctttg gccgtgaaac
     3181 gccccgtgtc ggcgaactct tagccgatta tttcggagaa accgaggctc acgcacttag
     3241 gaacgcactg gcgcaatcaa gcgatcccaa acgccctgct ctcatctttg ggtggagaga
     3301 tggactgaca ggccgtactt tcgacataag ccttcaccgg catgacggaa ccagtataat
     3361 tgagtttgaa ccggccgccg ctgagcaagc agacaacccc cttcgcctga ccagacaaat
     3421 tatcgcccgc actaaggagc tgaaatcgct ggaagaaatg gccgctcgtg ttcccaggta
     3481 tcttcaggca atgctgggtt atcatagggt aatgctctac agattcgccg acgatggctc
     3541 tggtatggta ataggggagg ccaagcgatc cgacctggag tcttttctgg ggcagcattt
     3601 ccccgcatct ctagtacccc agcaggcaag gcttttgtac ctcaagaatg ccatccgcgt
     3661 ggtatcggac tcccgcggta ttagtagcag gatcgttcca gaacacgacg cctctggagc
     3721 tgcgctggat ctgtctttcg ctcacctccg atctatcagc ccttgtcatt tagagtttct
     3781 gcgaaatatg ggggtcagcg caagtatgtc cctgagcata ataatcgatg gaacactatg
     3841 gggcctaata atttgccatc attacgaacc tagagcagta ccaatggcac agcgagtggc
     3901 cgccgaaatg ttcgccgatt ttttgtctct tcattttact gccgctcacc atcagcgcgg
     3961 ccacgggact ggctcgacag gttctggcag tagtgggact gcttcatcag aagataataa
     4021 catggccatg gccagaaaag tggacctgac cagttgtgac cgagagccga tacacattcc
     4081 tggctctatc cagccatgtg ggtgtttgct ggcctgcgat gcccaggcag tcaggattac
     4141 tcgaattacc gaaaatgcgg gtgccttctt tggccgtgaa acgccccgtg tcggcgaact
     4201 cttagccgat tatttcggag aaaccgaggc tcacgcactt aggaacgcac tggcgcaatc
     4261 aagcgatccc aaacgccctg ctctcatctt tgggtggaga gatggactga caggccgtac
     4321 tttcgacata agccttcacc ggcatgacgg aaccagtata attgagtttg aaccggccgc
     4381 cgctgagcaa gcagacaacc cccttcgcct gaccagacaa attatcgccc gcactaagga
     4441 gctgaaatcg ctggaagaaa tggccgctcg tgttcccagg tatcttcagg caatgctggg
     4501 ttatcatagg gtaatgctct acagattcgc cgacgatggc tctggtatgg taatagggga
     4561 ggccaagcga tccgacctgg agtcttttct ggggcagcat ttccccgcat ctctagtacc
     4621 ccagcaggca aggcttttgt acctcaagaa tgccatccgc gtggtatcgg actcccgcgg
     4681 tattagtagc aggatcgttc cagaacacga cgcctctgga gctgcgctgg atctgtcttt
     4741 cgctcacctc cgatctatca gcccttgtca tttagagttt ctgcgaaata tgggggtcag
     4801 cgcaagtatg tccctgagca taataatcga tggaacacta tggggcctaa taatttgcca
     4861 tcattacgaa cctagagcag taccaatggc acagcgagtg gccgccgaaa tgttcgccga
     4921 ttttttgtct cttcatttta ctgccgctca ccatcagcgc ggatccgcgt ttaaactcga
     4981 gggaagaggt agcctgctaa cttgcgggga cgtagaggaa aatcctggtc ccatgccaag
     5041 taggaagttt gttgagggtg aagtggtcag aggccgatgg cctgggagct ccctttatta
     5101 tgaagtagag attctgagcc acgacaacaa atcccagctc tacactgtaa aatacaaaga
     5161 tggcaccgaa ctggaactga aggagagtga tatcaagcct ttaaaatcct ttaagcaaag
     5221 aaaaagtggc tcgatttcca gctctccctc tcgacgccgt ggcagccgca gccgcagccg
     5281 ctcaagatca cgttcccgat ccccgggcag agcaccgaaa ggctccagaa gatccgtctc
     5341 tgcttcccat gagggtgacg tgaaggagaa gaaggagaag gagatgagga gggaaattct
     5401 gcaagttaag ctgactccgc ttgtgttgaa gccgttcgga aacagcgtca gcgtgtacaa
     5461 cggggagccc gaacacatgg agaagaacgc tacgccgtat aaagacaagc aggaaagaat
     5521 cattttgtca acagaagaca gatatatagt tacacagtac agccttcgtc ccaggagaga
     5581 agaggtcaaa gccaaagaaa tagagtctga ggaacaaaac cttgtcacca aaggacccgc
     5641 acctttggga acctttcaag tgaccactcc acagaggaag gacttggagt ttggaggagt
     5701 acctggtgcg gtcctcatca tgctgggcct gcccgcctgc gtcctcttgc tgctgctgca
     5761 gtgcaggcag aaggacccgg ggctgctcca cttccctcca cctctgccgg ctctccacga
     5821 gctgtgggaa cccagggtgt gcggcgttta cctcctctgg tttttcgttc aagctctctt
     5881 ccaccttcta ccagttggga aggttgcaga aggaacacct cttgttgatg gaagaagact
     5941 ccagtataga ctgaatggat tgtatgcctt cattctgaca tctgcagccc tgggagcagc
     6001 tgtcttctgg ggtgttgagc tgtgctacct gtacactcac ttcctgcagt tggcactggc
     6061 agccaccggg ttctccgtgc tcctgagtgc ttacctctat gttcgctctc tgcgagcccc
     6121 gagggaggag ctgtcgccag ccagttccgg aaatgctgtc tacgacttct tcattggccg
     6181 agagttaaac ccgcgacttg gtgctttcga tctcaagttc ttttgtgagt tgcgccctgg
     6241 attgattgga tgggtggtta ttaacttagt gatgcttctg atggaaatga aaattcagga
     6301 gcgagctgct ccgtccttgg caatgatctt ggttaacagt ttccagctct tatatgtggt
     6361 ggacgccctc tggaatgagg aagcactgct gacctccatg gacatcatgc acgacggctt
     6421 tggcttcatg ctggcctttg gggatttagt gtgggttcca tttacctaca gcctccaggc
     6481 cttttacctg gtcagccatc cccatgactt gtcctggcca ttggcttctg tcatcattgc
     6541 tctgaaattg tgtggatatg taatcttccg ttgtgcaaac tctcagaaaa atgcattccg
     6601 gaagaatccc actgatccaa agcttgcaca tttgaagacc attcatactt ccacggggaa
     6661 aagtctgctt gtttccggat ggtggggctt tgttcgccat cccaattact tgggtgacct
     6721 catcatggct ctggcgtggt ccctcccatg tggtttcaac cacctcctgc catacttcta
     6781 cattatctac ttcaccgcct tgcttatcca tcgagaggcc cgggatgaac accagtgcag
     6841 gaggaagtac ggcctggcct gggagaagta ctgccagcga gtgccctacc gcatattccc
     6901 ctacatttac tgagcggccg catagataac tgatccagtg tgctggaatt aattcgctgt
     6961 ctgcgagggc cagctgttgg ggtgagtact ccctctcaaa agcgggcatg acttctgcgc
     7021 taagattgtc agtttccaaa aacgaggagg atttgatatt cacctggccc gcggtgatgc
     7081 ctttgagggt ggccgcgtcc atctggtcag aaaagacaat ctttttgttg tcaagcttga
     7141 ggtgtggcag gcttgagatc tggccataca cttgagtgac aatgacatcc actttgcctt
     7201 tctctccaca ggtgtccact cccaggtcca actgcaggtc gagcatgcat ctagggcggc
     7261 caattccgcc cctctccccc ccccccctct ccctcccccc cccctaacgt tactggccga
     7321 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     7381 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     7441 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     7501 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     7561 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     7621 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     7681 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     7741 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     7801 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct
     7861 tgccacaacc cgggataatt cctgcagcca atatgggatc ggccattgaa caagatggat
     7921 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     7981 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     8041 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     8101 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     8161 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     8221 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     8281 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     8341 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag ggactcgcgc
     8401 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgatgat ctcgtcgtga
     8461 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     8521 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     8581 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     8641 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagggg
     8701 atcaattctc tagataactg atcataatca gccataccac atttgtagag gttttacttg
     8761 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     8821 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     8881 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     8941 tatcttaacg cgtcgagtgc attctagttg tggtttgtcc aaactcatca atgtatctta
     9001 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatctcgac atactagtta
     9061 aaagttttgt tactttatag aagaaatttt gagtttttgt ttttttttaa taaataaata
     9121 aacataaata aattgtttgt tgaatttatt attagtatgt aagtgtaaat ataataaaac
     9181 ttaatatcta ttcaaattaa taaataaacc tcgatataca gaccgataaa acacatgcgt
     9241 caattttacg catgattatc tttaacgtac gtcacaatat gattatcttt ctagggttaa
     9301 tctagtatac gcgtatgcgg ccgcttaatt aatccagctt ttgttccctt tagtgagggt
     9361 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     9421 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     9481 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     9541 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     9601 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     9661 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     9721 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     9781 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     9841 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     9901 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     9961 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    10021 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    10081 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    10141 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    10201 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
    10261 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    10321 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    10381 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    10441 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    10501 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    10561 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    10621 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    10681 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    10741 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    10801 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    10861 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    10921 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    10981 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    11041 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    11101 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    11161 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    11221 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    11281 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    11341 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    11401 tactcatact cttccttttt caatattatt gaagcattta tcagggtt
//
