LOCUS       sequence-16983-e         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  TITLE     MyoD
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CMV-myctagged MyoD
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        49..67
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     49..66
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             117..146
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             156..185
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             195..224
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             234..263
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             273..302
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             336..365
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gctcgtttag ggaccgccat tctgcctggg gacgtcggag caagcttgat ttaggtgaca
       61 ctatagaata caagctactt gttctttttg caggatccca tcgatttaaa gctatggagc
      121 aaaagctcat ttctgaagag gacttgaatg aaatggagca aaagctcatt tctgaagagg
      181 acttgaatga aatggagcaa aagctcattt ctgaagagga cttgaatgaa atggagcaaa
      241 agctcatttc tgaagaggac ttgaatgaaa tggagcaaaa gctcatttct gaagaggact
      301 tgaatgaaat ggagagcttg ggcgacctca ccatggagca aaagctcatt tctgaagagg
      361 acttgaattc caggaactgg gatatggagc ttctatcgcc gccactccgg gacatagact
      421 tgacaggccc cgacggctct ctctgctcct ttgagacagc agacgacttc tatgatgatc
      481 cgtgtttcga ctcaccagac ctgcgctttt ttgaggacct ggacccgcgc ctggtgcacg
      541 tgggagccct cctgaaaccg gaggagcacg cacacttctc tactgcggtg cacccaggcc
      601 caggcgctcg tgaggatgag catgtgcgcg cgcccagcgg gcacaccagg cgggtcgctg
      661 cttgctgtgg gcctgcaagg cgtgcaag
//
