LOCUS       sequence-169835-       11064 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sleeping beauty transposon for hydrodynamic tail vein injection for 
            CreER expression and tetracyclin-dependent YAP expression (CMV 
            promoter ) in mouse liver.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11064)
  AUTHORS   Hubner EK, Lechler C, Kohnke-Ertel B, Zmoos AF, Sage J, Schmid RM, 
            Ehmer U
  TITLE     An in vivo transfection system for inducible gene expression and 
            gene silencing in murine hepatocytes.
  JOURNAL   J Gene Med. 2016 Dec 23. doi: 10.1002/jgm.2940.
  PUBMED    28009940
REFERENCE   2  (bases 1 to 11064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gene Med.
            2016 Dec 23. doi: 10.1002/jgm.2940."
COMMENT     Sequence Label: pTC-CMV-Tet-YAP
FEATURES             Location/Qualifiers
     source          1..11064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(153..176)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      176..185
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(210..233)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(277..591)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    351..369
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    387..405
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    422..440
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    458..476
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    494..512
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    529..547
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    565..583
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(623..647)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(724..1434)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(804..827)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(1002..1021)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1271..1289
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(1456..1509)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(1534..2469)
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             complement(2482..3510)
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     primer_bind     complement(3546..3565)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     4725..4745
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(5127..5144)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5127..5143)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5136..5158)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5352..5371)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5471..5493
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5531..5549)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5617..5721
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5722..5790,5791..6582)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5940..5959)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6753..7341
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7242..7261
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7495..7512
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7629..7650
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7665..7670,7671..7688,7689..7695)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7703..7719
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7708..7730
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7727..7743
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7727..7743
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(join(8172..8180,8181..8216,8217..8219,8220..8255,
                     8256..8258,8259..8294,8295..8300,8301..8918))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(9005..9208)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        9209..9512
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    9598..9622
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gccgcctggg tcgccgcggg tgccggttgc ccgggtccgg acggcgggcc ctgcccctgc
       61 gggggctgcg aaggcggctg cccttggccc tggggggccg gttgaggcgg cggctgctgc
      121 ccgggatcct taggtaccga ttcgagcctt gctttatcgt catcgtcttt gtagtccatg
      181 gtggctaagc ttgaattcgc ggccgcaagc ttgtcgtcat cgtctttgta gtccatggta
      241 gatcaattct gacggtcact aaacgagctc actagtggcg atctgacggt tcactaaacg
      301 agctctgctt atataggcct cccaccgtac acgcctacct cgacatacgt tctctatcac
      361 tgatagggag taaactcgac atacgttctc tatcactgat agggataaac tcgacatacg
      421 ttctctatca ctgataggga gtaaactcga catacgttct ctatcactga tagggagtaa
      481 actcgacata cgttctctat cactgatagg gagtaaactc gacatcgttc tctatcactg
      541 atagggagta aactcgacat acgttctcta tcactgatag ggagtaaact cgggatccag
      601 tcgactgaat tggttccttt aaagcctgct tttttgtaca aacttgttga taactgtcta
      661 gaattaacca aactggatct ctgctgtccc tgtttaaaac gaattcatgc aattcatgcg
      721 catctacttg tacagctcgt ccatgccgcc ggtggagtgg cggccctcgg cgcgctcgta
      781 ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg tccagcttga tgttgacgtt
      841 gtaggcgccg ggcagctgca cgggcttctt ggccttgtag gtggtcttga cctcggcgtc
      901 gtagtggccg ccgtccttca gcttcagcct ctgcttgatc tcgcccttca gggcgccgtc
      961 ctcggggtac atccgctcgg aggaggcctc ccagcccatg gtcttcttct gcattacggg
     1021 gccgtcggag gggaagttgg tgccgcgcag cttcaccttg tagatgaact cgccgtcctg
     1081 cagggaggag tcctgggtca cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc
     1141 ccacttgaag ccctcgggga aggacagctt caagtagtcg gggatgtcgg cggggtgctt
     1201 cacgtaggcc ttggagccgt acatgaactg aggggacagg atgtcccagg cgaagggcag
     1261 ggggccgccc ttggtcacct tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc
     1321 gccctcgccc tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa
     1381 gcgcatgaac tccttgatga tggccatgtt atcctcctcg cccttgctca ccatccatac
     1441 gccggcgatt cgtcttgggc caggattctc ctcgacgtca ccgcatgtta gcagacttcc
     1501 tctgccctct ccactgcctt cacgcgtagc agctgtggca gggaaaccct ctgcctcccc
     1561 cgtgatgtaa tacttttgca aggaatgcga tgaagtagag cccgcagtgg ccaagtggct
     1621 ttggtccgtc tcctccacgg atgcccctcc acggctagtg ggcgcatgta ggcggtgggc
     1681 gtccgccgcc tccagcagca ggtcatagag gggcaccacg ttcttgcact tcatgctgta
     1741 cagatgctcc atgcctttgt tactcatgtg cctgatgtgg gagaggatga ggaggagctg
     1801 ggccagccgc tggtgctgct gctgcagggt caggcctgcc ttggccatca ggtggatcaa
     1861 agtgtctgtg atcttgtcca ggactcggtg gatatggtcc ttctcttcca gagacttcag
     1921 ggtgctggac agaaatgtgt acactccaga attaagcaaa ataatagatt tgaggcacac
     1981 aaactcctct ccctgcagat tcatcatgcg gaaccgagat gatgtagcca gcagcatgtc
     2041 gaagatctcc accatgccct ctacacattt tccctggttc ctgtccaaga gcaagttagg
     2101 agcaaacagt agcttcactg ggtgctccat ggagcgccag acgagaccaa tcatcaggat
     2161 ctctagccag gcacattcaa gaaggtggac ctgatcatgg agggtcaaat ccacaaagcc
     2221 tggcaccctc ttcgcccagt tgatcatgtg aaccagctcc ctgtctgcca ggttggtcag
     2281 taagcccatc atcgaagctt cactgaaggg tctggtagga tcatactcgg aatagagtat
     2341 ggggggctca gcatccaaca aggcactgac catctggtcg gccgtcaggg acaaggccag
     2401 gctgttcttc ttagagcgtt tgatcatgag cgggcttggc caaaggttgg cagctctcat
     2461 gtctccagca gatggctcga gatcgccatc ttccagcagg cgcaccattg cccctgtttc
     2521 actatccagg ttacggatat agttcatgac aatatttaca ttggtccagc caccagcttg
     2581 catgatctcc ggtattgaaa ctccagcgcg ggccatatct cgcgcggctc cgacacgggc
     2641 actgtgtcca gaccaggcca ggtatctctg accagagtca tccttagcgc cgtaaatcaa
     2701 tcgatgagtt gcttcaaaaa tcccttccag ggcgcgagtt gatagctggc tggtggcaga
     2761 tggcgcggca acaccatttt ttctgacccg gcaaaacagg tagttattcg gatcatcagc
     2821 tacaccagag acggaaatcc atcgctcgac cagtttagtt acccccaggc taagtgcctt
     2881 ctctacacct gcggtgctaa ccagcgtttt cgttctgcca atatggatta acattctccc
     2941 accgtcagta cgtgagatat ctttaaccct gatcctggca atttcggcta tacgtaacag
     3001 ggtgttataa gcaatcccca gaaatgccag attacgtata tcctggcagc gatcgctatt
     3061 ttccatgagt gaacgaacct ggtcgaaatc agtgcgttcg aacgctagag cctgttttgc
     3121 acgttcaccg gcatcaacgt tttcttttcg gatccgccgc ataaccagtg aaacagcatt
     3181 gctgtcactt ggtcgtggca gcccggaccg acgatgaagc atgtttagct ggcccaaatg
     3241 ttgctggata gtttttactg ccagaccgcg cgcctgaaga tatagaagat aatcgcgaac
     3301 atcttcaggt tctgcgggaa accatttccg gttattcaac ttgcaccatg ccgcccacga
     3361 ccggcaaacg gacagaagca ttttccaggt atgctcagaa aacgcctggc gatccctgaa
     3421 catgtccatc aggttcttgc gaacctcatc actcgttgca tcgaccggta atgcaggcaa
     3481 attttggtgt acggtcagta aattggacat ggtccgctta attaaggttg gacctgggag
     3541 tggacacctg tggagagaaa ggcaaagtgg atgtcattgt cactcaagtg tatggtcaga
     3601 tcgatctcaa gcctgccaca cctcaagctt gacaacaaaa agattgtctt ttctgaccag
     3661 atggacgcgg ccaccctcaa aggcatcacc gcgggccagg tgaatatcaa atcctcctcg
     3721 tttttggaaa ctgacaatct tagcgcagaa gtcatgcccg cttttgagag ggagtactca
     3781 ccccaacagc tggccctcgc agacagcgaa tttcgtacga taacctcctc aggttgctag
     3841 cagattgtga aagtggtcga cgactagaat tcgatatcaa gcttatcgat accgtcgagg
     3901 ccgcagatca gggggatcat tcactgtccc aggtcagtgg tggtgcctga agctgaggag
     3961 acagggccct gtcctcgtcc gtatttaagc agtggatcca gaggggcaac gggggaggct
     4021 gctggtgaat attaaccaag gtcaccccag ttatcggagg agcaaacagg ggctaagtcc
     4081 actggctggg atctgagtcg cccgcctacg ctgcccggac gctttgcctg ggcagtgtac
     4141 agcttccact gcacttaccg aaaggagtca ttgtacctgg ctcagaaacc acagcgtcct
     4201 gtgtccaagg tggagggggt ggcgtgagtc agacagtctc tgggagagta ccacttagct
     4261 ggccctctgc tctcactgca gaatccttag tggctgttcc actggtagca agatccccgg
     4321 gtacccctct cacactacct aaaccacgcc aggacaacct ctgctcctct ccaccgaaat
     4381 tccaaggggt cgagtggatg ttggaggtgg catgggccca gagaggtctc tgacctctgc
     4441 cccagctcca aggtcagcag gcagggaggg ctgtgtgttt gctgtttgct gcttgcaatg
     4501 tttgcccatt ttagggacat gagtaggctg aagtttgttc agtgtggact tcagaggcag
     4561 cacacaaaca gctgctggag gatgggaact gaggggttgg aagggggcag ggtgagccca
     4621 gaaactcctg tgtgcctctg agcctaactc cggagggttt cgcgcgcgaa agggatgcat
     4681 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac
     4741 attccacaga attccttgaa atacatccac aggtacacct ccaattgact caaatgatgt
     4801 caattagtct atcagaagct tctaaagcca tgacatcatt ttctggaatt ttccaagctg
     4861 tttaaaggca cagtcaactt agtgtatgta aacttctgac ccactggaat tgtgatacag
     4921 tgaattataa gtgaaataat ctgtctgtaa acaattgttg gaaaaatgac ttgtgtcatg
     4981 cacaaagtag atgtcctaac tgacttgcca aaactattgt ttgttaacaa gaaatttgtg
     5041 gagtagttga aaaacgagtt ttaatgactc caacttaagt gtatgtaaac ttccgacttc
     5101 aactgtaggg taccgagctc gaattcactg gccgtcgttt tacaacgtcg tgactgggaa
     5161 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     5221 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     5281 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     5341 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     5401 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     5461 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     5521 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     5581 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     5641 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     5701 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     5761 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     5821 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     5881 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     5941 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     6001 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     6061 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     6121 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     6181 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     6241 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     6301 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     6361 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     6421 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     6481 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     6541 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     6601 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     6661 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6721 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6781 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6841 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     6901 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6961 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7021 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7081 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7141 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7201 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7261 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7321 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7381 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     7441 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     7501 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     7561 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     7621 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     7681 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     7741 gaccatgatt acgccaagct tgcatgcctg caggtcgact ctagacctat acagttgaag
     7801 tcggaagttt acatacactt aagttggagt cattaaaact cgtttttcaa ctactccaca
     7861 aatttcttgt taacaaacaa tagttttggc aagtcagtta ggacatctac tttgtgcatg
     7921 acacaagtca tttttccaac aattgtttac agacagatta tttcacttat aattcactgt
     7981 atcacaattc cagtgggtca gaagtttaca tacactaagt tgactgtgcc tttaaacagc
     8041 ttggaaaatt ccagaaaatg atgtcatggc tttagaagct tctgatagac taattgacat
     8101 catttgagtc aattggaggt gtacctgtgg atgtatttca aggggatcca aatttgctcg
     8161 agcttactta gttacccggg gagcatgtca aggtcaaaat cgtcaagagc gtcagcaggc
     8221 agcatatcaa ggtcaaagtc gtcaagggca tcggctggga gcatgtctaa gtcaaaatcg
     8281 tcaagggcgt cggtcggccc gccgctttcg cactttagct gtttctccag gccacatatg
     8341 attagttcca ggccgaaaag gaaggcaggt tcggctccct gccggtcgaa cagctcaatt
     8401 gcttgtctca gaagtggggg catagaatcg gtggtaggtg tctctctttc ctcttttgct
     8461 acttgatgct cctgttcctc caatacgcag cccagtgtaa agtggcccac ggcggacaga
     8521 gcgtacagtg cgttctccag ggagaagcct tgctgacaca ggaacgcgag ctgattttcc
     8581 agggtttcgt actgtttctc tgttgggcgg gtgccgagat gcactttagc cccgtcgcga
     8641 tgtgagagga gagcacagcg gtatgacttg gcgttgttcc gcagaaagtc ttgccatgac
     8701 tcgccttcca gggggcagaa gtgggtatga tgcctgtcca gcatctcgat tggcagggca
     8761 tcgagcaggg cccgcttgtt cttcacgtgc cagtacaggg taggctgctc aactcccagc
     8821 ttttgagcga gtttccttgt cgtcaggcct tcgataccga caccattgag taattccaga
     8881 gctccgttta tgactttgct cttgtccagt ctagacatgg tgaattcgac tgcagaattc
     8941 gaagcttgag ctcgagatct gagtccggta gcgctagcgg cgcgccctga cggttcacta
     9001 aacgagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
     9061 gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc
     9121 cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc
     9181 cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt
     9241 actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt
     9301 taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca
     9361 agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg
     9421 ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc
     9481 caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta
     9541 atgaccccgt aattgattac tattaattaa ccatctttat ggctcgagat gttatcaacc
     9601 actttgtaca agaaagctgg gtctagatat ctcgacggag gggtcacagg gatgccaccc
     9661 gggatcctct agactcgagc ggccgccact gtgctggata tctgcagaat tcagtctgcc
     9721 tgagggctct ataaccatgt aagaaagctt tctttatcta gcttggtggc agccaaaaca
     9781 gactccatgt cattaaggat gtcagaactc aaagcttcct gcagacttgg catcagctcc
     9841 tctccttcta tgttcattcc atctccttcc agtgttccaa ggtccacatt tgtcccagga
     9901 atggcttcaa ggtagtctgg gaaacggttc tgctgtgagg gcagggtgct ttggttgata
     9961 gtatcacctg tatccatctc atccacactg ttcaggaagt catctggggt tcgagggaca
    10021 ctgtagctgc tcatgcttag tccactgtct gtactctcat ctcgagagtg ataggtgcca
    10081 ctgttaagga aaggatctga gctattggtc gtcattgttc tcaattcctg agacatcccg
    10141 ggagaagaca ctggattttg agtcccacca tcctgctcca gtgttggtaa ctggctacgc
    10201 agggctaact cctgccgaag cagttcttgc tgtttcagcc gcagcctctc cttctccatc
    10261 tgcagttgct gcagtcgcat ctgttgctgc tggttggagt tgctgccacc catgacgcct
    10321 ccctgtgggc tctggggagc caggggtggt ggctgtttca ctggagcact ctgactgatt
    10381 ctctggttca tggcaaaacg agggtcaagc cttgggtcta gccaagaggt ggtcttgttc
    10441 ttatggttta tatagtaaat ttctccatcc tgagtcatgg cttgttccca tccatcagga
    10501 agaggacctg aagccgagtt catcatattc tgctgcactg gtggactggt gggggctgtg
    10561 acgttcatct gggacagcat ggccttcctg gggtcctgcc atgttgttgt ctgatcgatg
    10621 tgatttaaga agtatctctg accagaagat gtctttgcca tctcccaacc tgctggcaga
    10681 ggtacatcat caggtatctc aaaagaagac tgtcgaagat gctgagctgt gggtgtagct
    10741 gctgggccag agactactcc agtgggggtc agtgtcccag gagaaacagc tcccaactgc
    10801 agagaagctg gagaggaatg agctcgaaca tgctgtggag tcagggctcc tgcagtgcct
    10861 gcatcagtac tggcctgtcg ggagtgggat ttgggctccg gcggcttgaa gaaggagtcg
    10921 ggcagcttcc ggagcctcat gggcacggtc tggggcacgt tggccgtctt ggggttcatg
    10981 acggcgttga agagcgcctc caggtcggtc tccgagtccc cgcggacgtg cacgatctga
    11041 tgcccggcgg ggggtgcctg cggc
//
