LOCUS       sequence-169837-       11609 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sleeping beauty transposon for hydrodynamic tail vein injection for 
            CreER expression and tetracyclin-dependent YAP expression 
            (ApoE.HCR.hAAT promoter) in mouse liver.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11609)
  AUTHORS   Hubner EK, Lechler C, Kohnke-Ertel B, Zmoos AF, Sage J, Schmid RM, 
            Ehmer U
  TITLE     An in vivo transfection system for inducible gene expression and 
            gene silencing in murine hepatocytes.
  JOURNAL   J Gene Med. 2016 Dec 23. doi: 10.1002/jgm.2940.
  PUBMED    28009940
REFERENCE   2  (bases 1 to 11609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gene Med.
            2016 Dec 23. doi: 10.1002/jgm.2940."
COMMENT     Sequence Label: pTC-ApoE-Tet-YAP
FEATURES             Location/Qualifiers
     source          1..11609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     7..25
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(63..85)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     185..204
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     398..420
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     412..429
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     413..429
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(811..831)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             2046..3074
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             3087..4022
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             4047..4100
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4122..4832
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4267..4285)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4535..4554
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4729..4752
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    4908..4932
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4964..5278
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    4972..4990
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5008..5026
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5043..5061
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5079..5097
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5115..5133
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5150..5168
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5186..5204
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     5251..5275
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5322..5345
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      5370..5379
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5379..5402
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(6997..7021)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(8247..8864,8865..8870,8871..8906,8907..8909,8910..8945,
                     8946..8948,8949..8984,8985..8993)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(9422..9438)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9422..9438)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9435..9457)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9446..9462
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9470..9476,9477..9494,9495..9500))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9515..9536
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9653..9670)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9824..10412)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9904..9923)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10583..11374,11375..11443))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11206..11225
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11444..11548)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
       61 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
      121 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
      181 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
      241 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
      301 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      361 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      421 acggccagtg aattcgagct cggtacccta cagttgaagt cggaagttta catacactta
      481 agttggagtc attaaaactc gtttttcaac tactccacaa atttcttgtt aacaaacaat
      541 agttttggca agtcagttag gacatctact ttgtgcatga cacaagtcat ttttccaaca
      601 attgtttaca gacagattat ttcacttata attcactgta tcacaattcc agtgggtcag
      661 aagtttacat acactaagtt gactgtgcct ttaaacagct tggaaaattc cagaaaatga
      721 tgtcatggct ttagaagctt ctgatagact aattgacatc atttgagtca attggaggtg
      781 tacctgtgga tgtatttcaa ggaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
      841 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tccctttcgc gcgcgaaacc
      901 ctccggagtt aggctcagag gcacacagga gtttctgggc tcaccctgcc cccttccaac
      961 ccctcagttc ccatcctcca gcagctgttt gtgtgctgcc tctgaagtcc acactgaaca
     1021 aacttcagcc tactcatgtc cctaaaatgg gcaaacattg caagcagcaa acagcaaaca
     1081 cacagccctc cctgcctgct gaccttggag ctggggcaga ggtcagagac ctctctgggc
     1141 ccatgccacc tccaacatcc actcgacccc ttggaatttc ggtggagagg agcagaggtt
     1201 gtcctggcgt ggtttaggta gtgtgagagg ggtacccggg gatcttgcta ccagtggaac
     1261 agccactaag gattctgcag tgagagcaga gggccagcta agtggtactc tcccagagac
     1321 tgtctgactc acgccacccc ctccaccttg gacacaggac gctgtggttt ctgagccagg
     1381 tacaatgact cctttcggta agtgcagtgg aagctgtaca ctgcccaggc aaagcgtccg
     1441 ggcagcgtag gcgggcgact cagatcccag ccagtggact tagcccctgt ttgctcctcc
     1501 gataactggg gtgaccttgg ttaatattca ccagcagcct cccccgttgc ccctctggat
     1561 ccactgctta aatacggacg aggacagggc cctgtctcct cagcttcagg caccaccact
     1621 gacctgggac agtgaatgat ccccctgatc tgcggcctcg acggtatcga taagcttgat
     1681 atcgaattct agtcgtcgac cactttcaca atctgctagc aacctgagga ggttatcgta
     1741 cgaaattcgc tgtctgcgag ggccagctgt tggggtgagt actccctctc aaaagcgggc
     1801 atgacttctg cgctaagatt gtcagtttcc aaaaacgagg aggatttgat attcacctgg
     1861 cccgcggtga tgcctttgag ggtggccgcg tccatctggt cagaaaagac aatctttttg
     1921 ttgtcaagct tgaggtgtgg caggcttgag atcgatctga ccatacactt gagtgacaat
     1981 gacatccact ttgcctttct ctccacaggt gtccactccc aggtccaacc ttaattaagc
     2041 ggaccatgtc caatttactg accgtacacc aaaatttgcc tgcattaccg gtcgatgcaa
     2101 cgagtgatga ggttcgcaag aacctgatgg acatgttcag ggatcgccag gcgttttctg
     2161 agcatacctg gaaaatgctt ctgtccgttt gccggtcgtg ggcggcatgg tgcaagttga
     2221 ataaccggaa atggtttccc gcagaacctg aagatgttcg cgattatctt ctatatcttc
     2281 aggcgcgcgg tctggcagta aaaactatcc agcaacattt gggccagcta aacatgcttc
     2341 atcgtcggtc cgggctgcca cgaccaagtg acagcaatgc tgtttcactg gttatgcggc
     2401 ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa acaggctcta gcgttcgaac
     2461 gcactgattt cgaccaggtt cgttcactca tggaaaatag cgatcgctgc caggatatac
     2521 gtaatctggc atttctgggg attgcttata acaccctgtt acgtatagcc gaaattgcca
     2581 ggatcagggt taaagatatc tcacgtactg acggtgggag aatgttaatc catattggca
     2641 gaacgaaaac gctggttagc accgcaggtg tagagaaggc acttagcctg ggggtaacta
     2701 aactggtcga gcgatggatt tccgtctctg gtgtagctga tgatccgaat aactacctgt
     2761 tttgccgggt cagaaaaaat ggtgttgccg cgccatctgc caccagccag ctatcaactc
     2821 gcgccctgga agggattttt gaagcaactc atcgattgat ttacggcgct aaggatgact
     2881 ctggtcagag atacctggcc tggtctggac acagtgcccg tgtcggagcc gcgcgagata
     2941 tggcccgcgc tggagtttca ataccggaga tcatgcaagc tggtggctgg accaatgtaa
     3001 atattgtcat gaactatatc cgtaacctgg atagtgaaac aggggcaatg gtgcgcctgc
     3061 tggaagatgg cgatctcgag ccatctgctg gagacatgag agctgccaac ctttggccaa
     3121 gcccgctcat gatcaaacgc tctaagaaga acagcctggc cttgtccctg acggccgacc
     3181 agatggtcag tgccttgttg gatgctgagc cccccatact ctattccgag tatgatccta
     3241 ccagaccctt cagtgaagct tcgatgatgg gcttactgac caacctggca gacagggagc
     3301 tggttcacat gatcaactgg gcgaagaggg tgccaggctt tgtggatttg accctccatg
     3361 atcaggtcca ccttcttgaa tgtgcctggc tagagatcct gatgattggt ctcgtctggc
     3421 gctccatgga gcacccagtg aagctactgt ttgctcctaa cttgctcttg gacaggaacc
     3481 agggaaaatg tgtagagggc atggtggaga tcttcgacat gctgctggct acatcatctc
     3541 ggttccgcat gatgaatctg cagggagagg agtttgtgtg cctcaaatct attattttgc
     3601 ttaattctgg agtgtacaca tttctgtcca gcaccctgaa gtctctggaa gagaaggacc
     3661 atatccaccg agtcctggac aagatcacag acactttgat ccacctgatg gccaaggcag
     3721 gcctgaccct gcagcagcag caccagcggc tggcccagct cctcctcatc ctctcccaca
     3781 tcaggcacat gagtaacaaa ggcatggagc atctgtacag catgaagtgc aagaacgtgg
     3841 tgcccctcta tgacctgctg ctggaggcgg cggacgccca ccgcctacat gcgcccacta
     3901 gccgtggagg ggcatccgtg gaggagacgg accaaagcca cttggccact gcgggctcta
     3961 cttcatcgca ttccttgcaa aagtattaca tcacggggga ggcagagggt ttccctgcca
     4021 cagctgctac gcgtgaaggc agtggagagg gcagaggaag tctgctaaca tgcggtgacg
     4081 tcgaggagaa tcctggccca agacgaatcg ccggcgtatg gatggtgagc aagggcgagg
     4141 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
     4201 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     4261 agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt
     4321 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
     4381 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     4441 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
     4501 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
     4561 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
     4621 agcagaggct gaagctgaag gacggcggcc actacgacgc cgaggtcaag accacctaca
     4681 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ctggacatca
     4741 cctcccacaa cgaggactac accatcgtgg aacagtacga gcgcgccgag ggccgccact
     4801 ccaccggcgg catggacgag ctgtacaagt agatgcgcat gaattgcatg aattcgttta
     4861 aacagggaca gcagagatcc agtttggtta attctagaca gttatcaaca agtttgtaca
     4921 aaaaagcagg ctttaaagga accaattcag tcgactggat cccgagttta ctccctatca
     4981 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacga tgtcgagttt
     5041 actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg
     5101 tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttat ccctatcagt
     5161 gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgaggtag
     5221 gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgccac
     5281 tagtgagctc gtttagtgac cgtcagaatt gatctaccat ggactacaaa gacgatgacg
     5341 acaagcttgc ggccgcgaat tcaagcttag ccaccatgga ctacaaagac gatgacgata
     5401 aagcaaggct cgaatcggta cctaaggatc ccgggcagca gccgccgcct caaccggccc
     5461 cccagggcca agggcagccg ccttcgcagc ccccgcaggg gcagggcccg ccgtccggac
     5521 ccgggcaacc ggcacccgcg gcgacccagg cggcgccgca ggcacccccc gccgggcatc
     5581 agatcgtgca cgtccgcggg gactcggaga ccgacctgga ggcgctcttc aacgccgtca
     5641 tgaaccccaa gacggccaac gtgccccaga ccgtgcccat gaggctccgg aagctgcccg
     5701 actccttctt caagccgccg gagcccaaat cccactcccg acaggccagt actgatgcag
     5761 gcactgcagg agccctgact ccacagcatg ttcgagctca ttcctctcca gcttctctgc
     5821 agttgggagc tgtttctcct gggacactga cccccactgg agtagtctct ggcccagcag
     5881 ctacacccac agctcagcat cttcgacagt cttcttttga gatacctgat gatgtacctc
     5941 tgccagcagg ttgggagatg gcaaagacat cttctggtca gagatacttc ttaaatcaca
     6001 tcgatcagac aacaacatgg caggacccca ggaaggccat gctgtcccag atgaacgtca
     6061 cagcccccac cagtccacca gtgcagcaga atatgatgaa ctcggcttca ggtcctcttc
     6121 ctgatggatg ggaacaagcc atgactcagg atggagaaat ttactatata aaccataaga
     6181 acaagaccac ctcttggcta gacccaaggc ttgaccctcg ttttgccatg aaccagagaa
     6241 tcagtcagag tgctccagtg aaacagccac cacccctggc tccccagagc ccacagggag
     6301 gcgtcatggg tggcagcaac tccaaccagc agcaacagat gcgactgcag caactgcaga
     6361 tggagaagga gaggctgcgg ctgaaacagc aagaactgct tcggcaggag ttagccctgc
     6421 gtagccagtt accaacactg gagcaggatg gtgggactca aaatccagtg tcttctcccg
     6481 ggatgtctca ggaattgaga acaatgacga ccaatagctc agatcctttc cttaacagtg
     6541 gcacctatca ctctcgagat gagagtacag acagtggact aagcatgagc agctacagtg
     6601 tccctcgaac cccagatgac ttcctgaaca gtgtggatga gatggataca ggtgatacta
     6661 tcaaccaaag caccctgccc tcacagcaga accgtttccc agactacctt gaagccattc
     6721 ctgggacaaa tgtggacctt ggaacactgg aaggagatgg aatgaacata gaaggagagg
     6781 agctgatgcc aagtctgcag gaagctttga gttctgacat ccttaatgac atggagtctg
     6841 ttttggctgc caccaagcta gataaagaaa gctttcttac atggttatag agccctcagg
     6901 cagactgaat tctgcagata tccagcacag tggcggccgc tcgagtctag aggatcccgg
     6961 gtggcatccc tgtgacccct ccgtcgagat atctagaccc agctttcttg tacaaagtgg
     7021 ttgataacat ctcgagccat aaagatggtt aattaagtta ggctcagagg cacacaggag
     7081 tttctgggct caccctgccc ccttccaacc cctcagttcc catcctccag cagctgtttg
     7141 tgtgctgcct ctgaagtcca cactgaacaa acttcagcct actcatgtcc ctaaaatggg
     7201 caaacattgc aagcagcaaa cagcaaacac acagccctcc ctgcctgctg accttggagc
     7261 tggggcagag gtcagagacc tctctgggcc catgccacct ccaacatcca ctcgacccct
     7321 tggaatttcg gtggagagga gcagaggttg tcctggcgtg gtttaggtag tgtgagaggg
     7381 gtacccgggg atcttgctac cagtggaaca gccactaagg attctgcagt gagagcagag
     7441 ggccagctaa gtggtactct cccagagact gtctgactca cgccaccccc tccaccttgg
     7501 acacaggacg ctgtggtttc tgagccaggt acaatgactc ctttcggtaa gtgcagtgga
     7561 agctgtacac tgcccaggca aagcgtccgg gcagcgtagg cgggcgactc agatcccagc
     7621 cagtggactt agcccctgtt tgctcctccg ataactgggg tgaccttggt taatattcac
     7681 cagcagcctc ccccgttgcc cctctggatc cactgcttaa atacggacga ggacagggcc
     7741 ctgtctcctc agcttcaggc accaccactg acctgggaca gtgaatgatc cccctgatct
     7801 gcggcctcga cggtatcgat aagcttgata tcgaattcta gtcgtcgacc actttcacaa
     7861 tctgctagca acctgaggag gttatcgtac gaaattcgct gtctgcgagg gccagctgtt
     7921 ggggtgagta ctccctctca aaagcgggca tgacttctgc gctaagattg tcagtttcca
     7981 aaaacgagga ggatttgata ttcacctggc ccgcggtgat gcctttgagg gtggccgcgt
     8041 ccatctggtc agaaaagaca atctttttgt tgtcaagctt gaggtgtggc aggcttgaga
     8101 tcgatctgac catacacttg agtgacaatg acatccactt tgcctttctc tccacaggtg
     8161 tccactccca ggtccaacgg cgcgccgcta gcgctaccgg actcagatct cgagctcaag
     8221 cttcgaattc tgcagtcgaa ttcaccatgt ctagactgga caagagcaaa gtcataaacg
     8281 gagctctgga attactcaat ggtgtcggta tcgaaggcct gacgacaagg aaactcgctc
     8341 aaaagctggg agttgagcag cctaccctgt actggcacgt gaagaacaag cgggccctgc
     8401 tcgatgccct gccaatcgag atgctggaca ggcatcatac ccacttctgc cccctggaag
     8461 gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc ataccgctgt gctctcctct
     8521 cacatcgcga cggggctaaa gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa
     8581 ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag aacgcactgt
     8641 acgctctgtc cgccgtgggc cactttacac tgggctgcgt attggaggaa caggagcatc
     8701 aagtagcaaa agaggaaaga gagacaccta ccaccgattc tatgccccca cttctgagac
     8761 aagcaattga gctgttcgac cggcagggag ccgaacctgc cttccttttc ggcctggaac
     8821 taatcatatg tggcctggag aaacagctaa agtgcgaaag cggcgggccg accgacgccc
     8881 ttgacgattt tgacttagac atgctcccag ccgatgccct tgacgacttt gaccttgata
     8941 tgctgcctgc tgacgctctt gacgattttg accttgacat gctccccggg taactaagta
     9001 agctcgagca aatttggatc cccttgaaat acatccacag gtacacctcc aattgactca
     9061 aatgatgtca attagtctat cagaagcttc taaagccatg acatcatttt ctggaatttt
     9121 ccaagctgtt taaaggcaca gtcaacttag tgtatgtaaa cttctgaccc actggaattg
     9181 tgatacagtg aattataagt gaaataatct gtctgtaaac aattgttgga aaaatgactt
     9241 gtgtcatgca caaagtagat gtcctaactg acttgccaaa actattgttt gttaacaaga
     9301 aatttgtgga gtagttgaaa aacgagtttt aatgactcca acttaagtgt atgtaaactt
     9361 ccgacttcaa ctgtataggt ctagagtcga cctgcaggca tgcaagcttg gcgtaatcat
     9421 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     9481 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     9541 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     9601 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     9661 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     9721 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     9781 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     9841 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     9901 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     9961 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    10021 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    10081 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    10141 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    10201 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    10261 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    10321 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    10381 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    10441 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    10501 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    10561 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    10621 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    10681 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    10741 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    10801 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    10861 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    10921 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    10981 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    11041 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    11101 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    11161 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    11221 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    11281 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    11341 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    11401 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    11461 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    11521 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
    11581 aagaaaccat tattatcatg acattaacc
//
