LOCUS       sequence-169840-       10655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sleeping beauty transposon for hydrodynamic tail vein injection for 
            CreER expression and tetracyclin-dependent transgene/shRNA 
            expression (CMV promoter) in mouse liver.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10655)
  AUTHORS   Hubner EK, Lechler C, Kohnke-Ertel B, Zmoos AF, Sage J, Schmid RM, 
            Ehmer U
  TITLE     An in vivo transfection system for inducible gene expression and 
            gene silencing in murine hepatocytes.
  JOURNAL   J Gene Med. 2016 Dec 23. doi: 10.1002/jgm.2940.
  PUBMED    28009940
REFERENCE   2  (bases 1 to 10655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gene Med.
            2016 Dec 23. doi: 10.1002/jgm.2940."
COMMENT     Sequence Label: pTC-CMV-Tet
FEATURES             Location/Qualifiers
     source          1..10655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             132..185
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             207..917
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(352..370)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     620..639
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     814..837
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    994..1118
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1143..1148,1149..1166,1167..1173)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1227..1886
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1293..1312)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2228..2533
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2412..2431
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2574..2698)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     enhancer        2784..3087
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3088..3291
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3242..3262
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(3378..3995,3996..4001,4002..4037,4038..4040,4041..4076,
                     4077..4079,4080..4115,4116..4124)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4553..4569)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4553..4569)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4566..4588)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4577..4593
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4601..4607,4608..4625,4626..4631))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4646..4667
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4784..4801)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4955..5543)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5035..5054)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5714..6505,6506..6574))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6337..6356
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6575..6679)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6747..6765
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6803..6825)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6925..6944
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7138..7160
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7152..7169
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7153..7169
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7551..7571)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     8731..8750
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             8786..9814
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             join(9827..10655,1..107)
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
ORIGIN
        1 ccgtggagga gacggaccaa agccacttgg ccactgcggg ctctacttca tcgcattcct
       61 tgcaaaagta ttacatcacg ggggaggcag agggtttccc tgccacagct gctacgcgtg
      121 aaggcagtgg agagggcaga ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg
      181 gcccaagacg aatcgccggc gtatggatgg tgagcaaggg cgaggaggat aacatggcca
      241 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
      301 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
      361 aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
      421 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
      481 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
      541 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
      601 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
      661 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
      721 tgaaggacgg cggccactac gacgccgagg tcaagaccac ctacaaggcc aagaagcccg
      781 tgcagctgcc cggcgcctac aacgtcaaca tcaagctgga catcacctcc cacaacgagg
      841 actacaccat cgtggaacag tacgagcgcg ccgagggccg ccactccacc ggcggcatgg
      901 acgagctgta caagtagatg cgcatgaatt gcatgaattc gttttaaaca gggacagcag
      961 agatccagtt tggttaattc tagacagtta tcaacaagtt tgtacaaaaa agctgaacga
     1021 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact
     1081 acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag
     1141 gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccgtcga
     1201 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
     1261 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
     1321 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
     1381 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
     1441 aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
     1501 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg
     1561 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
     1621 cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga
     1681 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
     1741 ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc
     1801 atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact
     1861 gcgatgagtg gcagggcggg gcgtaaagat ctggatccgg cttactaaaa gccagataac
     1921 agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc
     1981 gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga
     2041 cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc
     2101 atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg
     2161 atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc
     2221 tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt
     2281 ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag
     2341 tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga
     2401 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga
     2461 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
     2521 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg
     2581 actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
     2641 atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggttg
     2701 ataacatctc gagccataaa gatggttaat taatagtaat caattacggg gtcattagtt
     2761 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     2821 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     2881 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     2941 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     3001 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     3061 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     3121 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     3181 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     3241 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
     3301 aaccgtcagg gcgcgccgct agcgctaccg gactcagatc tcgagctcaa gcttcgaatt
     3361 ctgcagtcga attcaccatg tctagactgg acaagagcaa agtcataaac ggagctctgg
     3421 aattactcaa tggtgtcggt atcgaaggcc tgacgacaag gaaactcgct caaaagctgg
     3481 gagttgagca gcctaccctg tactggcacg tgaagaacaa gcgggccctg ctcgatgccc
     3541 tgccaatcga gatgctggac aggcatcata cccacttctg ccccctggaa ggcgagtcat
     3601 ggcaagactt tctgcggaac aacgccaagt cataccgctg tgctctcctc tcacatcgcg
     3661 acggggctaa agtgcatctc ggcacccgcc caacagagaa acagtacgaa accctggaaa
     3721 atcagctcgc gttcctgtgt cagcaaggct tctccctgga gaacgcactg tacgctctgt
     3781 ccgccgtggg ccactttaca ctgggctgcg tattggagga acaggagcat caagtagcaa
     3841 aagaggaaag agagacacct accaccgatt ctatgccccc acttctgaga caagcaattg
     3901 agctgttcga ccggcaggga gccgaacctg ccttcctttt cggcctggaa ctaatcatat
     3961 gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc gaccgacgcc cttgacgatt
     4021 ttgacttaga catgctccca gccgatgccc ttgacgactt tgaccttgat atgctgcctg
     4081 ctgacgctct tgacgatttt gaccttgaca tgctccccgg gtaactaagt aagctcgagc
     4141 aaatttggat ccccttgaaa tacatccaca ggtacacctc caattgactc aaatgatgtc
     4201 aattagtcta tcagaagctt ctaaagccat gacatcattt tctggaattt tccaagctgt
     4261 ttaaaggcac agtcaactta gtgtatgtaa acttctgacc cactggaatt gtgatacagt
     4321 gaattataag tgaaataatc tgtctgtaaa caattgttgg aaaaatgact tgtgtcatgc
     4381 acaaagtaga tgtcctaact gacttgccaa aactattgtt tgttaacaag aaatttgtgg
     4441 agtagttgaa aaacgagttt taatgactcc aacttaagtg tatgtaaact tccgacttca
     4501 actgtatagg tctagagtcg acctgcaggc atgcaagctt ggcgtaatca tggtcatagc
     4561 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     4621 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     4681 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     4741 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     4801 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     4861 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     4921 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     4981 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     5041 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     5101 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     5161 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     5221 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     5281 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     5341 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     5401 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     5461 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     5521 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     5581 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     5641 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     5701 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     5761 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     5821 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     5881 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     5941 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     6001 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     6061 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     6121 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     6181 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     6241 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     6301 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     6361 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     6421 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     6481 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     6541 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     6601 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     6661 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     6721 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     6781 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     6841 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     6901 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     6961 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     7021 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     7081 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     7141 agtcacgacg ttgtaaaacg acggccagtg aattcgagct cggtacccta cagttgaagt
     7201 cggaagttta catacactta agttggagtc attaaaactc gtttttcaac tactccacaa
     7261 atttcttgtt aacaaacaat agttttggca agtcagttag gacatctact ttgtgcatga
     7321 cacaagtcat ttttccaaca attgtttaca gacagattat ttcacttata attcactgta
     7381 tcacaattcc agtgggtcag aagtttacat acactaagtt gactgtgcct ttaaacagct
     7441 tggaaaattc cagaaaatga tgtcatggct ttagaagctt ctgatagact aattgacatc
     7501 atttgagtca attggaggtg tacctgtgga tgtatttcaa ggaattctgt ggaatgtgtg
     7561 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     7621 tccctttcgc gcgcgaaacc ctccggagtt aggctcagag gcacacagga gtttctgggc
     7681 tcaccctgcc cccttccaac ccctcagttc ccatcctcca gcagctgttt gtgtgctgcc
     7741 tctgaagtcc acactgaaca aacttcagcc tactcatgtc cctaaaatgg gcaaacattg
     7801 caagcagcaa acagcaaaca cacagccctc cctgcctgct gaccttggag ctggggcaga
     7861 ggtcagagac ctctctgggc ccatgccacc tccaacatcc actcgacccc ttggaatttc
     7921 ggtggagagg agcagaggtt gtcctggcgt ggtttaggta gtgtgagagg ggtacccggg
     7981 gatcttgcta ccagtggaac agccactaag gattctgcag tgagagcaga gggccagcta
     8041 agtggtactc tcccagagac tgtctgactc acgccacccc ctccaccttg gacacaggac
     8101 gctgtggttt ctgagccagg tacaatgact cctttcggta agtgcagtgg aagctgtaca
     8161 ctgcccaggc aaagcgtccg ggcagcgtag gcgggcgact cagatcccag ccagtggact
     8221 tagcccctgt ttgctcctcc gataactggg gtgaccttgg ttaatattca ccagcagcct
     8281 cccccgttgc ccctctggat ccactgctta aatacggacg aggacagggc cctgtctcct
     8341 cagcttcagg caccaccact gacctgggac agtgaatgat ccccctgatc tgcggcctcg
     8401 acggtatcga taagcttgat atcgaattct agtcgtcgac cactttcaca atctgctagc
     8461 aacctgagga ggttatcgta cgaaattcgc tgtctgcgag ggccagctgt tggggtgagt
     8521 actccctctc aaaagcgggc atgacttctg cgctaagatt gtcagtttcc aaaaacgagg
     8581 aggatttgat attcacctgg cccgcggtga tgcctttgag ggtggccgcg tccatctggt
     8641 cagaaaagac aatctttttg ttgtcaagct tgaggtgtgg caggcttgag atcgatctga
     8701 ccatacactt gagtgacaat gacatccact ttgcctttct ctccacaggt gtccactccc
     8761 aggtccaacc ttaattaagc ggaccatgtc caatttactg accgtacacc aaaatttgcc
     8821 tgcattaccg gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg acatgttcag
     8881 ggatcgccag gcgttttctg agcatacctg gaaaatgctt ctgtccgttt gccggtcgtg
     8941 ggcggcatgg tgcaagttga ataaccggaa atggtttccc gcagaacctg aagatgttcg
     9001 cgattatctt ctatatcttc aggcgcgcgg tctggcagta aaaactatcc agcaacattt
     9061 gggccagcta aacatgcttc atcgtcggtc cgggctgcca cgaccaagtg acagcaatgc
     9121 tgtttcactg gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa
     9181 acaggctcta gcgttcgaac gcactgattt cgaccaggtt cgttcactca tggaaaatag
     9241 cgatcgctgc caggatatac gtaatctggc atttctgggg attgcttata acaccctgtt
     9301 acgtatagcc gaaattgcca ggatcagggt taaagatatc tcacgtactg acggtgggag
     9361 aatgttaatc catattggca gaacgaaaac gctggttagc accgcaggtg tagagaaggc
     9421 acttagcctg ggggtaacta aactggtcga gcgatggatt tccgtctctg gtgtagctga
     9481 tgatccgaat aactacctgt tttgccgggt cagaaaaaat ggtgttgccg cgccatctgc
     9541 caccagccag ctatcaactc gcgccctgga agggattttt gaagcaactc atcgattgat
     9601 ttacggcgct aaggatgact ctggtcagag atacctggcc tggtctggac acagtgcccg
     9661 tgtcggagcc gcgcgagata tggcccgcgc tggagtttca ataccggaga tcatgcaagc
     9721 tggtggctgg accaatgtaa atattgtcat gaactatatc cgtaacctgg atagtgaaac
     9781 aggggcaatg gtgcgcctgc tggaagatgg cgatctcgag ccatctgctg gagacatgag
     9841 agctgccaac ctttggccaa gcccgctcat gatcaaacgc tctaagaaga acagcctggc
     9901 cttgtccctg acggccgacc agatggtcag tgccttgttg gatgctgagc cccccatact
     9961 ctattccgag tatgatccta ccagaccctt cagtgaagct tcgatgatgg gcttactgac
    10021 caacctggca gacagggagc tggttcacat gatcaactgg gcgaagaggg tgccaggctt
    10081 tgtggatttg accctccatg atcaggtcca ccttcttgaa tgtgcctggc tagagatcct
    10141 gatgattggt ctcgtctggc gctccatgga gcacccagtg aagctactgt ttgctcctaa
    10201 cttgctcttg gacaggaacc agggaaaatg tgtagagggc atggtggaga tcttcgacat
    10261 gctgctggct acatcatctc ggttccgcat gatgaatctg cagggagagg agtttgtgtg
    10321 cctcaaatct attattttgc ttaattctgg agtgtacaca tttctgtcca gcaccctgaa
    10381 gtctctggaa gagaaggacc atatccaccg agtcctggac aagatcacag acactttgat
    10441 ccacctgatg gccaaggcag gcctgaccct gcagcagcag caccagcggc tggcccagct
    10501 cctcctcatc ctctcccaca tcaggcacat gagtaacaaa ggcatggagc atctgtacag
    10561 catgaagtgc aagaacgtgg tgcccctcta tgacctgctg ctggaggcgg cggacgccca
    10621 ccgcctacat gcgcccacta gccgtggagg ggcat
//
