LOCUS       sequence-169844-       10952 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of guide RNA (hup53_Ex4) with fluorescent GFP 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10952)
  AUTHORS   Herold
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: FgH1tUTG_hup53_Ex4
FEATURES             Location/Qualifiers
     source          1..10952
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..142
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(61..80)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     307..327
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(336..764)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(446..467)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     658..677
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    810..1034
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1023..1040
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             1066..1246
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1756..2344
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1862..1873
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2271..2291
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(2384..3097,3098..3100,3101..3103))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2387..3097,3098..3100,3101..3103))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2429..2450)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2778..2797
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3037..3058
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(3104..3157)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(3167..3787)
                     /codon_start=1
                     /gene="synthetic gene"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     promoter        complement(3825..5036)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(3969..3988)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_RNA        complement(5072..5147)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    5173..5191
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(5236..5255)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_feature    5446..5563
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5861..5905)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6090..6323
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6816..6941
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6988..7168
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7186..7384)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7211..7231)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7386..7765
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     7937..7956
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8031..8135
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8136..8204,8205..8996)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8354..8373)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9167..9755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9656..9675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9909..9926
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10043..10064
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10079..10084,10085..10102,10103..10109)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10117..10133
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10122..10144
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10141..10157
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10141..10157
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    10206..10327
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10217..10236)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10271..10290
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(10457..10831)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(10850..10897)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(join(10945..10952,1..322))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag gcagaggcgg
       61 cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac
      121 tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat
      181 tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg
      241 ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact
      301 ttccacaccc taactgacac acattccaca gaattaattc gcgttaaatt tttgttaaat
      361 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
      421 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
      481 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
      541 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
      601 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
      661 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
      721 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtggggat accccctaga
      781 gccccagctg gttctttccg cctcagaagc catagagccc accgcatccc cagcatgcct
      841 gctattgtct tcccaatcct cccccttgct gtcctgcccc accccacccc ccagaataga
      901 atgacaccta ctcagacaat gcgatgcaat ttcctcattt tattaggaaa ggacagtggg
      961 agtggcacct tccagggtca aggaaggcac gggggagggg caaacaacag atggctggca
     1021 actagaaggc acagtcgagg ctgatcagcg ggtttaaacg ggccctgcta gagattttcc
     1081 acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac
     1141 acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt
     1201 agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta cagtccggat
     1261 gcagctctcg ggccatgtga tgaaatgcta ggcggctgtc aaacctccac tctaatactt
     1321 ctctctccgg gtcatccatc ccatgcaggc tcacagggtg taacaagcgg gtgttctctc
     1381 cttcattggc ttcttctacc ttctcttgct caactggtac tagcttgtag caccatccaa
     1441 aggtcagtgg atatctgatc cctggccctg gtgtgtagtt ctgccaatca gggaagtagc
     1501 cttgtgtgtg gtagatccac agatcaagga tatcttgtct tcgttgggag tgaattagcc
     1561 cttccagtcc ccccttttct tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca
     1621 ttggtcttaa aggtacctga ggtgtgactg gaaaacccac ctcctcctcc tcttgtgctt
     1681 ctagccaggc acaatcagca ttggtagctg ctgtattgct acttgtgatt gctccatgtt
     1741 tttctaggtc tcgatgcggg gaggcggccc aaagggagat ccgactcgtc tgagggcgaa
     1801 ggcgaagacg cggaagaggc cgcagagccg gcagcaggcc gcgggaagga aggtccgctg
     1861 gattgagggc cgaagggacg tagcagaagg acgtcccgcg cagaatccag gtggcaacac
     1921 aggcgagcag ccaaggaaag gacgatgatt tccccgacaa caccacggaa ttgtcagtgc
     1981 ccaacagccg agcccctgtc cagcagcggg caaggcaggc ggcgatgagt tccgccgtgg
     2041 caatagggag ggggaaagcg aaagtcccgg aaaggagctg acaggtggtg gcaatgcccc
     2101 aaccagtggg ggttgcgtca gcaaacacag tgcacaccac gccacgttgc ctgacaacgg
     2161 gccacaactc ctcataaaga gacagcaacc aggatttata caaggaggag aaaatgaaag
     2221 ccatacggga agcaatagca tgatacaaag gcattaaagc agcgtatcca catagcgtaa
     2281 aaggagcaac atagttaaga ataccagtca atctttcaca aattttgtaa tccagaggtt
     2341 gattatcgat aagcttgata tcgaattgta gatcgcggcc gctttacttg tacagctcgt
     2401 ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct
     2461 tctcgttggg gtctttgctc agggcggact gggtgctcag gtagtggttg tcgggcagca
     2521 gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc
     2581 cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg
     2641 ccatgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc
     2701 cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga
     2761 acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct
     2821 ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt
     2881 agcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg
     2941 gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct
     3001 cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga
     3061 tgggcaccac cccggtgaac agctcctcgc ccttgctcac cattgggcca ggattctcct
     3121 cgacgtcacc gcatgttagc agacttcctc tgccctcacc ggacccagag ccagactcac
     3181 atttcagctg cttttccagg ccacagatga tcagttccag gccaaaaagg aatgcaggct
     3241 cagccccttg gtggtcaaac aactcaatag cttgccgcag cagtggtggc atggagtcag
     3301 tggtgggggt ctccctctcc tccttggcaa cttggtgctc ctggtcctcc agcacacagc
     3361 ccaaggtgaa gtggcccaca gcagagaggg catacaaggc attttccagg gagaagcctt
     3421 gttggcacag gaatgccagc tggttctcca gggtctcata ctgcttctct gtaggcctgg
     3481 tgcccaagtg aactttggca ccatcccggt gggagagcaa ggcacatctg aagctcttgg
     3541 cattgttcct caggaagtcc tgccaggact ccccttccag agggcagaag tgggtgtggt
     3601 gcctgtccaa catctcaatg gccagggcat caagcagagc cctcttgttc ttcacatgcc
     3661 agtacagggt aggctgctcc acacccagct tctgggccag cttcctggtg gtcaagccct
     3721 caatgccaac ttcattcagg agctccaagg cagagttgat gactttgctc ttgtccagtc
     3781 tggacatggt ggatcctcta gagtcgacct gcagcccaag cttcgtctaa caaaaaagcc
     3841 aaaaacggcc agaatttagc ggacaattta ctagtctaac actgaaaatt acatattgac
     3901 ccaaatgatt acatttcaaa aggtgcctaa aaaacttcac aaaacacact cgccaacccc
     3961 gagcgcatag ttcaaaaccg gagcttcagc tacttaagaa gataggtaca taaaaccgac
     4021 caaagaaact gacgcctcac ttatccctcc cctcaccaga ggtccggcgc ctgtcgattc
     4081 aggagagcct accctaggcc cgaaccctgc gtcctgcgac ggagaaaagc ctaccgcaca
     4141 cctaccggca ggtggcccca ccctgcatta taagccaaca gaacgggtga cgtcacgaca
     4201 cgacgagggc gcgcgctccc aaaggtacgg gtgcactgcc caacggcacc gccataactg
     4261 ccgcccccgc aacagacgac aaaccgagtt ctccagtcag tgacaaactt cacgtcaggg
     4321 tccccagatg gtgccccagc ccatctcacc cgaataagag ctttcccgca ttagcgaagg
     4381 cctcaagacc ttgggttctt gccgcccacc atgcccccca ccttgtttca acgacctcac
     4441 agcccgcctc acaagcgtct tccattcaag actcgggaac agccgccatt ttgctgcgct
     4501 ccccccaacc cccagttcag ggcaaccttg ctcgcggacc cagactacag cccttggcgg
     4561 tctctccaca cgcttccgtc ccaccgagcg gcccggcggc cacgaaagcc ccggccagcc
     4621 cagcagcccg ctactcacca agtgacgatc acagcgatcc acaaacaaga accgcgaccc
     4681 aaatcccggc tgcgacggaa ctagctgtgc cacacccggc gcgtccttat ataatcatcg
     4741 gcgttcaccg ccccacggag atccctccgc agaatcgccg agaagggact acttttcctc
     4801 gcctgttccg ctctctggaa agaaaaccag tgccctagag tcacccaagt cccgtcctaa
     4861 aatgtccttc tgctgatact ggggttctaa ggccgagtct tatgagcagc gggccgctgt
     4921 cctgagcgtc cgggcggaag gatcaggacg ctcgctgcgc ccttcgtctg acgtggcagc
     4981 gctcgccgtg aggagggggg cgcccgcggg aggcgccaaa acccggcgcg gaggccagat
     5041 ctggagccga cacgggttaa ttaaaaaaaa agcaccgact cggtgccact ttttcaagtt
     5101 gataacggac tagccttatt ttaacttgct atttctagct ctaaaacaga cggaaaccgt
     5161 agctgccggg aatctctatc actgataggg acttataaga ttcccaaatc caaagacatt
     5221 tcacgtttat ggtgatttcc cagaacacat agcgacatgc aaatattgca gggcgccact
     5281 cccctgtccc tcacagccat cttcctgcca gggcgcacgc gcgctgggtg ttcccgccta
     5341 gtgacactgg gcccgcgatt ccttggagcg ggttgatgac gtcagcgttc gaattccatg
     5401 gttaattaac caaactggat ctctgctgtc cctgtaataa acccgaaaat tttgaatttt
     5461 tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt gctattatgt ctactattct
     5521 ttcccctgca ctgtaccccc caatcccccc ttttctttta aaattgtgga tgaatactgc
     5581 catttgtctg cagaattggc gcacgcagtg ccgatccgtt cactaatcga atggatctgt
     5641 ctctgtctct ctctccacct tcttcttcta ttccttcggg cctgtcgggt cccctcgggg
     5701 ttgggaggtg ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag
     5761 aaagtacagc aaaaactatt cttaaaccta ccaagcctcc tactatcatt atgaataatt
     5821 ttatatacca cagccaattt gttatgttaa accaattcca caaacttgcc catttatcta
     5881 attccaataa ttcttgttca ttcttttctt gctggttttg cgattcttca attaaggagt
     5941 gtattaagct tgtgtaattg ttaatttctc tgtcccactc catccaggtc gtgtgattcc
     6001 aaatctgttc cagagattta ttactccaac tagcattcca aggcacagca gtggtgcaaa
     6061 tgagttttcc agagcaaccc caaatcccca ggagctgttg atcctttagg tatctttcca
     6121 cagccaggat tcttgcctgg agctgcttga tgccccagac tgtgagttgc aacagatgct
     6181 gttgcgcctc aatagccctc agcaaattgt tctgctgctg cactatacca gacaataatt
     6241 gtctggcctg taccgtcagc gtcattgacg ctgcgcccat agtgcttcct gctgctccca
     6301 agaacccaag gaacaaagct cctattccca ctgctctttt ttctctctgc accactcttc
     6361 tctttgcctt ggtgggtgct actcctaatg gttcaatttt tactacttta tatttatata
     6421 attcacttct ccaattgtcc ctcatatctc ctcctccagg tctgaagatc agcggccgct
     6481 tgctgtgcgg tggtcttact tttgttttgc tcttcctcta tcttgtctaa agcttccttg
     6541 gtgtctttta tctctatcct ttgatgcaca caatagaggg ttgctactgt attatataat
     6601 gatctaagtt cttctgatcc tgtctgaagg gatggttgta gctgtcccag tatttgtcta
     6661 cagccttctg atgtttctaa caggccagga ttaactgcga atcgttctag ctccctgctt
     6721 gcccatacta tatgttttaa tttatatttt ttctttcccc ctggccttaa ccgaattttt
     6781 tcccatcgcg atctaattct cccccgctta atactgacgc tctcgcaccc atctctctcc
     6841 ttctagcctc cgctagtcaa aatttttggc gtactcacca gtcgccgccc ctcgcctctt
     6901 gccgtgcgcg cttcagcaag ccgagtcctg cgtcgagaga gctcctctgg tttccctttc
     6961 gctttcaagt ccctgttcgg gcgccactgc tagagatttt ccacactgac taaaagggtc
     7021 tgagggatct ctagttacca gagtcacaca acagacgggc acacactact tgaagcactc
     7081 aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag agctcccagg
     7141 ctcagatctg gtctaaccag agagacccag tacaggcaaa acgcgctgct tatatagacc
     7201 tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt
     7261 ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga
     7321 cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc
     7381 atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat
     7441 aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg
     7501 ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac
     7561 tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt
     7621 attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt
     7681 tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat
     7741 taataactag tcaataatca atgtcaacgc gtatatctgg cccgtacatc gcgaagcagc
     7801 gcaaaacgcc taaccctaag cagattcttc atgcaattgt cggtcaagcc ttgccttgtt
     7861 gtagcttaaa ttttgctcgc gcactactca gcgacctcca acacacaagc agggagcaga
     7921 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca taggggatcg
     7981 ggagatctcc cgatccgtcg acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     8041 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     8101 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     8161 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     8221 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     8281 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     8341 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     8401 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     8461 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     8521 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     8581 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     8641 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     8701 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     8761 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     8821 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     8881 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     8941 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     9001 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     9061 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     9121 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     9181 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     9241 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     9301 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     9361 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     9421 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     9481 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     9541 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     9601 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     9661 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     9721 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     9781 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     9841 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     9901 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     9961 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
    10021 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    10081 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    10141 caggaaacag ctatgaccat gattacgcca agctctagct agaggtcgac ggtatacaga
    10201 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
    10261 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
    10321 acaagttggg gtgggcgaag aactccagca tgagatcccc gcgctggagg atcatccagc
    10381 cggcgtcccg gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga
    10441 aatctcgtag cacgtgtcag tcctgctcct cggccacgaa gtgcacgcag ttgccggccg
    10501 ggtcgcgcag ggcgaactcc cgcccccacg gctgctcgcc gatctcggtc atggccggcc
    10561 cggaggcgtc ccggaagttc gtggacacga cctccgacca ctcggcgtac agctcgtcca
    10621 ggccgcgcac ccacacccag gccagggtgt tgtccggcac cacctggtcc tggaccgcgc
    10681 tgatgaacag ggtcacgtcg tcccggacca caccggcgaa gtcgtcctcc acgaagtccc
    10741 gggagaaccc gagccggtcg gtccagaact cgaccgctcc ggcgacgtcg cgcgcggtga
    10801 gcaccggaac ggcactggtc aacttggcca tggtttagtt cctcaccttg tcgtattata
    10861 ctatgccgat atactatgcc gatgattaat tgtcaacacg tgctgatcag atccgaaaat
    10921 ggatatacaa gctcccggga gctttttgca aa
//
