LOCUS       sequence-169845-       10829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for Gal4 inducible human IL-2/MIP1a with 
            constitutive mCherry expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10829)
  AUTHORS   Roybal KT, Williams JZ, Morsut L, Rupp LJ, Kolinko I, Choe JH, 
            Walker WJ, McNally KA, Lim WA
  TITLE     Engineering T Cells with Customized Therapeutic Response Programs 
            Using Synthetic Notch Receptors.
  JOURNAL   Cell. 2016 Oct 6;167(2):419-432.e16. doi: 
            10.1016/j.cell.2016.09.011. Epub 2016 Sep 29.
  PUBMED    27693353
REFERENCE   2  (bases 1 to 10829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.09.011"; journalName: "Cell"; date: "2016-10-6-
            6"; volume: "167"; issue: "2"; pages: "419-432.e16"
COMMENT     Sequence Label: pHR_Gal4UAS_humanIL-2_T2A_MIP1-alpha_PGK_mCherry
FEATURES             Location/Qualifiers
     source          1..10829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             61..294
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(319..337)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(320..337)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(627..646)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(860..879)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     979..1001
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1039..1057)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1125..1229
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1230..1298,1299..2090)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1448..1467)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2261..2849
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2750..2769
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3003..3020
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3090..3110)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3095..3424
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3275..3410
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4558..4623
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4753..4773
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5198..5332
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5235..5254)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5289..5308
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5500..6133
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6180..6305
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6802..7035
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7220..7264
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7549..7666
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     7892..7916
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     sig_peptide     8006..8065
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             8477..8530
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     promoter        8932..9431
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8967..8990)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9364..9383
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9452..10162
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9597..9615)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     9865..9884
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    10239..10827
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10292..10312)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10710..10721)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cgataccgtc gaccaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
       61 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
      121 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      181 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      241 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct
      301 agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt
      361 tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc
      421 tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt
      481 tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc
      541 aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac
      601 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
      661 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
      721 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
      781 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
      841 tcgaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
      901 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
      961 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     1021 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     1081 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     1141 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     1201 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     1261 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     1321 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     1381 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     1441 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     1501 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     1561 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     1621 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     1681 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     1741 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     1801 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     1861 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     1921 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     1981 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     2041 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     2101 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     2161 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     2221 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2281 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2341 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2401 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2461 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2521 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2581 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2641 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2701 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2761 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2821 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2881 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2941 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3001 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     3061 cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     3121 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     3181 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3241 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     3301 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     3361 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     3421 caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg
     3481 cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg
     3541 cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag
     3601 ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc
     3661 agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta
     3721 ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg
     3781 tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt
     3841 gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac
     3901 cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt
     3961 cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac
     4021 ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta
     4081 atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat
     4141 agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc
     4201 tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg
     4261 gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac tggtatcgca
     4321 tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc
     4381 aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac gatacggtga
     4441 ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt gaaggaacct
     4501 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta
     4561 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
     4621 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
     4681 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
     4741 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
     4801 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
     4861 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta
     4921 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac
     4981 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta
     5041 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat
     5101 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     5161 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     5221 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5281 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
     5341 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
     5401 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
     5461 aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc
     5521 aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     5581 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
     5641 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
     5701 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
     5761 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
     5821 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
     5881 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
     5941 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     6001 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     6061 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     6121 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     6181 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     6241 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     6301 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     6361 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     6421 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     6481 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     6541 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     6601 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt
     6661 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     6721 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     6781 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     6841 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     6901 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     6961 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     7021 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     7081 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     7141 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     7201 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     7261 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     7321 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     7381 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     7441 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     7501 agtgaacgga tctcgacggt atcgccaaat ggcagtattc atccacaatt ttaaaagaaa
     7561 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     7621 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     7681 ggacagcaga gatccagttt ggatcgataa gcttgatatc gaattcggag cactgtcctc
     7741 cgaacgtcgg agcactgtcc tccgaacgtc ggagcactgt cctccgaacg tcggagcact
     7801 gtcctccgaa cggagcatgt cctccgaacg tcggagcact gtcctccgaa cgactagtta
     7861 ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct
     7921 ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctct
     7981 cgacattcgt tggatcgccg ccaccatgta caggatgcag ctgctcagtt gcatcgcctt
     8041 gtctctcgct ctcgtgacca atagtgcccc aacatccagc tcaaccaaga agactcagct
     8101 tcagttggaa catctgttgc tggatctgca gatgatcctt aatgggatta acaactacaa
     8161 gaaccccaaa ctgactagga tgctgacctt caaattctat atgccaaaaa aggcaacgga
     8221 actgaagcac ctccagtgtt tggaggaaga gctgaagccc ttggaggaag tccttaattt
     8281 ggcccagagc aaaaactttc acctgcgccc tagggacctg attagtaaca ttaatgtcat
     8341 tgttctcgaa ctcaaaggaa gtgaaaccac ttttatgtgc gagtacgcgg atgaaactgc
     8401 cactatcgtg gagttcttga atagatggat taccttttgc cagtctataa tctccacact
     8461 cacacggaag cgcagagagg gtcgaggttc cctgctgact tgtggcgacg tagaagagaa
     8521 tcctgggcct atgcaggtct caacggcagc tctggccgtc ctcctgtgca ccatggccct
     8581 gtgtaaccaa tttagcgcta gcctcgctgc cgacacccct accgcctgtt gtttttctta
     8641 caccagccgg cagatcccac agaactttat agctgactac tttgaaacaa gtagtcagtg
     8701 ttctaaacct ggagttatct tcttgacaaa gcgcagcagg caggtgtgcg ccgaccctag
     8761 tgaggagtgg gtccagaagt atgtgtctga tctggaactc tccgcctgag atccttgact
     8821 tgcggccgca actcccacct gcaacatgcg tgactgactg aggccgcgac tctagagtcg
     8881 acctgcatct aggcgccgga attagatctc tcgaggttaa cgaattctac cgggtagggg
     8941 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
     9001 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
     9061 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
     9121 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
     9181 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
     9241 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
     9301 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
     9361 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
     9421 cgggcctttc gacctgcagc ccaagcttac catggtgagc aagggcgagg aggataacat
     9481 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     9541 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     9601 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     9661 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     9721 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     9781 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     9841 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     9901 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     9961 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
    10021 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
    10081 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
    10141 catggacgag ctgtacaagt aaagcggccg cgactctaga gtcgacctgc aggcatgcaa
    10201 gcttgcaggc atgcaagctt gatatcaagc ttatcgataa tcaacctctg gattacaaaa
    10261 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
    10321 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
    10381 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
    10441 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
    10501 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
    10561 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
    10621 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
    10681 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
    10741 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
    10801 ggatctccct ttgggccgcc tccccgcat
//
