LOCUS       sequence-169846-        7220 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of constitutively active human RAR-alpha in chick 
            embryos.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7220)
  AUTHORS   Martinez-Morales PL, Diez del Corral R, Olivera-Martinez I, Quiroga 
            AC, Das RM, Barbas JA, Storey KG, Morales AV
  TITLE     FGF and retinoic acid activity gradients control the timing of 
            neural crest cell emigration in the trunk.
  JOURNAL   J Cell Biol. 2011 Aug 8;194(3):489-503. doi: 10.1083/jcb.201011077. 
            Epub 2011 Aug 1.
  PUBMED    21807879
REFERENCE   2  (bases 1 to 7220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201011077"; journalName: "J Cell Biol"; date: 
            "2011-08-8- 8"; volume: "194"; issue: "3"; pages: "489-503"
COMMENT     Sequence Label: pCIG-VP16–RAR-alpha
FEATURES             Location/Qualifiers
     source          1..7220
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     335..357
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     419..438
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1970..2203
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     misc_feature    join(2360..2934,2935..2937,2938..2946)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2527..2544)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2754..2773
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2962..2982
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2986..3006
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3010..3030
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3040..3042,3043..3045,3046..3759)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3040..3042,3043..3045,3046..3756)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3085..3106)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3346..3365)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3693..3714
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3851..3870)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3916..3971
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3970..3989)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4362..4378)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4362..4378)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4375..4397)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4386..4402
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4410..4416,4417..4434,4435..4440))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4455..4476
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4535..4730
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4581..4716
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4736..4870
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4773..4792)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4827..4846
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4938..4955)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5109..5697)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5189..5208)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5868..6659,6660..6728))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6491..6510
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6729..6833)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccymgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt
       61 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga
      121 cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct
      181 agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
      241 cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg
      301 gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc
      361 ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc
      421 aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattgctcga ggtcgatcga
      481 cggtatcgat aagcttgata gcttgatatc gaattagatc cgggcccagg ccccatgccc
      541 cgaggagggg tggtctgaag cccaccagag ccccctgcca gactgtctgc ctcccttctg
      601 actgtggccg cttggcatgg ccagcaacag cagctcctgc ccgacacctg ggggcgggca
      661 cctcaatggg tacccggtgc ctccctacgc cttcttcttc ccccctatgc tgggtggact
      721 ctccccgcca ggcgctctga ccactctcca gcaccagctt ccagttagtg gatatagcac
      781 accatcccca gccaccattg agacccagag cagcagttct gaagagatag tgcccagccc
      841 tccctcgcca ccccctctac cccgcatcta caagccttgc tttgtctgtc aggacaagtc
      901 ctcaggctac cactatgggg tcagcgcctg tgagggctgc aagggcttct tccgccgcag
      961 catccagaag aacatggtgt acacgtgtca ccgggacaag aactgcatca tcaacaaggt
     1021 gacccggaac cgctgccagt actgccgact gcagaagtgc tttgaagtgg gcatgtccaa
     1081 ggagtctgtg agaaacgacc gaaacaagaa gaagaaggag gtgcccaagc ccgagtgctc
     1141 tgagagctac acgctgacgc cggaggtggg ggagctcatt gagaaggtgc gcaaagcgca
     1201 ccaggaaacc ttccctgccc tctgccagct gggcaaatac actacgaaca acagctcaga
     1261 acaacgtgtc tctctggaca ttgacctctg ggacaagttc agtgaactct ccaccaagtg
     1321 catcattaag actgtggagt tcgccaagca gctgcccggc ttcaccaccc tcaccatcgc
     1381 cgaccagatc accctcctca aggctgcctg cctggacatc ctgatcctgc ggatctgcac
     1441 gcggtacacg cccgagcagg acaccatgac cttctcggac gggctgaccc tgaaccggac
     1501 ccagatgcac aacgctggct tcggccccct caccgacctg gtctttgcct tcgccaacca
     1561 gctgctgccc ctggagatgg atgatgcgga gacggggctg ctcagcgcca tctgcctcat
     1621 ctgcggagac cgccaggacc tggagcagcc ggaccgggtg gacatgctgc aggagccgct
     1681 gctggaggcg ctaaaggtct acgtgcggaa gcggaggccc agccgccccc acatgttccc
     1741 caagatgcta atgaagatta ctgacctgcg aagcatcagc gccaaggggg ctgagcgggt
     1801 gatcacgctg aagatggaga tcccgggctc catgccgcct ctcatccagg aaatgttgga
     1861 gaactcagag ggcctggaca ctctgagcgg acagccgggg ggtggggggc gggacggggg
     1921 tggcctggcc cccccgccag gcagctgtag ccccagcctc acggatctgg cccccccgac
     1981 cgatgtcagc ctgggggacg agctccactt agacggcgag gacgtggcga tggcgcatgc
     2041 cgacgcgcta gacgatttcg atctggacat gttgggggac ggggattccc cgggtccggg
     2101 atttaccccc cacgactccg ccccctacgg cgctctggat atggccgact tcgagtttga
     2161 gcagatgttt accgatgccc ttggaattga cgagtacggt gggtaggggg cgcgaccgga
     2221 cccgcatccc ccgtctgggt tttcccctcc cgtccccggt tcgtatccac aataaacacg
     2281 agcacataca ttacaaaaac ctgcggttgt cgtctgatta tttggtggat catcgaattc
     2341 ctgcagcccg ggggatccgc ccctctccct cccccccccc taacgttact ggccgaagcc
     2401 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     2461 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     2521 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     2581 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     2641 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     2701 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     2761 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     2821 ctgatctggg gcctcggtac acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     2881 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc
     2941 acaaccatgg tgcacgtgga tccaaaaaag aagagaaagg tagatccaaa aaagaagaga
     3001 aaggtagatc caaaaaagaa gagaaaggta cacgtgtcca tggtgagcaa gggcgaggag
     3061 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     3121 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     3181 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     3241 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     3301 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     3361 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     3421 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     3481 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     3541 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     3601 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     3661 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     3721 gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccgcaa ttcactcctc
     3781 aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat
     3841 accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc
     3901 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
     3961 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt
     4021 atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc
     4081 tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa
     4141 aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa
     4201 catccctaaa attttcctta catgttttac tagccagatt tttcctcctc tcctgactac
     4261 tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcac cgtcgaccag
     4321 ctggtcgacg gtgcaccgtc gaccagcttg gcgtaatcat ggtcatagct gtttcctgtg
     4381 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     4441 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     4501 ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag
     4561 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     4621 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     4681 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt
     4741 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     4801 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     4861 tgtctggatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     4921 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     4981 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     5041 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     5101 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     5161 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     5221 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     5281 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     5341 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     5401 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     5461 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     5521 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     5581 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     5641 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     5701 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     5761 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     5821 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     5881 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     5941 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     6001 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     6061 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     6121 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     6181 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     6241 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     6301 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     6361 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     6421 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     6481 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     6541 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     6601 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     6661 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     6721 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     6781 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     6841 ccccgaaaag tgccacctgg gtcgacattg attattgact agttattaat agtaatcaat
     6901 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     6961 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     7021 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     7081 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     7141 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     7201 tacttggcag tacatctacg
//
