LOCUS       sequence-16985-e         519 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Sullivan EK, Weirich CS, Guyon JR, Sif S, Kingston RE
  TITLE     Transcriptional activation domains of human heat shock factor 1 
            recruit human SWI/SNF.
  JOURNAL   Mol Cell Biol 2001 Sep;21(17):5826-37.
  PUBMED    11486022
REFERENCE   2  (bases 1 to 519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol"; date: "2001-09"; volume: "21(17)"; pages: "5826-37"
COMMENT     Sequence Label: pBABE HSF Flag wt
FEATURES             Location/Qualifiers
     source          1..519
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(50..73)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(74..445)
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GLSVDTSTLLDLVSPLVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
ORIGIN
        1 gctggcgatt cctttttttt agagcctgtc tgtttataga tcttcgctac ttgtcatcgt
       61 cgtccttgta gtcggagaca gtggggtcct tggctttggg aggctccgag cctgtcagca
      121 gggagatggt ggggtcctcg gcgaagccgt ccccttcgga gaagtaggag ccctctccca
      181 gctcaaacag caccggcagg tcgttgctcc cggtgtccac ggagccgggg tccagcagga
      241 acagcggctg cgctgtgtag tgcaccagct gcttccctga atccgggctg ctgttctctg
      301 cctcgggagg cctggggggc tcctggggag acaggagctc ttggatactg gccaggctgc
      361 tgtcaaggtc aggcaggctc atgtcgggca cggtcaccaa ggggctgacc aggtccagca
      421 gggtactggt gtccacactc aagccgtggc tgctcagcag ggtctgcagg ttatccaggt
      481 tggagtccat agcatcccac tggtcacgga gctccttct
//
