LOCUS       sequence-16987-e         466 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Sullivan EK, Weirich CS, Guyon JR, Sif S, Kingston RE
  TITLE     Transcriptional activation domains of human heat shock factor 1 
            recruit human SWI/SNF.
  JOURNAL   Mol Cell Biol 2001 Sep;21(17):5826-37.
  PUBMED    11486022
REFERENCE   2  (bases 1 to 466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol"; date: "2001-09"; volume: "21(17)"; pages: "5826-37"
COMMENT     Sequence Label: pBABE HSF Flag Ac
FEATURES             Location/Qualifiers
     source          1..466
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(40..63)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(64..435)
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDISALLALFSPSVTVGDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFALGAGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
ORIGIN
        1 cctttttttt agagcctgtc tgtttataga tcttcgctac ttgtcatcgt cgtccttgta
       61 gtcggagaca gtggggtcct tggctttggg aggctccgag cctgtcagca gggagatggt
      121 ggggtcctcg gcgaagccgt ccccttcgga gaagtaggag cccgctccca gcgcaaacag
      181 caccggcagg tcgttgctcc cggtgtccac ggagccgggg tccagcagga acagcggctg
      241 cgctgtgtag tgcaccagct gcttccctga atccgggctg ctgttctctg cctcgggagg
      301 cctggggggc tcctggggag acaggagctc ttggatactg gccaggctgc tgtcaaggtc
      361 aggcaggctc atgtcgccca cggtcaccga ggggctgaac agggccagca gggcactgat
      421 gtccacgctg aagccgtgac tgctcagcat ggtctgcagg ttatcc
//
