LOCUS       sequence-169873-       10952 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of guide RNA (muBim_Ex3) with fluorescent GFP 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10952)
  AUTHORS   Aubrey BJ, Kelly GL, Kueh AJ, Brennan MS, O'Connor L, Milla L, 
            Wilcox S, Tai L, Strasser A, Herold MJ
  TITLE     An inducible lentiviral guide RNA platform enables the 
            identification of tumor-essential genes and tumor-promoting 
            mutations in vivo.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1422-32. doi: 
            10.1016/j.celrep.2015.02.002. Epub 2015 Feb 26.
  PUBMED    25732831
REFERENCE   2  (bases 1 to 10952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.02.002"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1422-32"
COMMENT     Sequence Label: FgH1tUTG_muBim_Ex3
FEATURES             Location/Qualifiers
     source          1..10952
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             28..208
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(234..251)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    240..464
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      510..938
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(597..616)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     807..828
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(947..967)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        952..1281
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1132..1267
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1194..1213
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        1329..1376
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1395..1769
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1899..2020
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1936..1955)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1990..2009
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2069..2085)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2069..2085)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2082..2104)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2093..2109
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2117..2123,2124..2141,2142..2147))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2162..2183
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2300..2317)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2471..3059)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2551..2570)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3230..4021,4022..4090))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3853..3872
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4091..4195)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4270..4289)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4461..4840
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4842..5040
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4995..5015
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             5058..5238
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5285..5410
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5903..6136
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6321..6365
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6663..6780
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     6971..6990
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    7035..7053
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_RNA        7079..7154
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        7190..8401
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     8238..8257
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             8439..9059
                     /codon_start=1
                     /gene="synthetic gene"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     CDS             9069..9122
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(9123..9125,9126..9128,9129..9842)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9123..9125,9126..9128,9129..9839)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9168..9189)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9429..9448)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9776..9797
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    9882..10470
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9935..9955)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10353..10364)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg
       61 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
      121 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
      181 ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac
      241 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
      301 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
      361 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
      421 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
      481 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
      541 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
      601 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
      661 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
      721 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
      781 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
      841 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
      901 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
      961 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     1021 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     1081 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     1141 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     1201 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     1261 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     1321 cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg
     1381 tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg
     1441 tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg
     1501 acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc
     1561 aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg
     1621 ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg
     1681 agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg
     1741 tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg
     1801 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     1861 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     1921 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     1981 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     2041 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     2101 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     2161 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     2221 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2281 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     2341 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     2401 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     2461 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     2521 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2581 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2641 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     2701 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2761 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     2821 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2881 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     2941 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     3001 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     3061 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     3121 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     3181 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     3241 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     3301 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     3361 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     3421 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     3481 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     3541 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     3601 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     3661 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     3721 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     3781 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     3841 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     3901 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     3961 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     4021 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     4081 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     4141 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     4201 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg
     4261 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
     4321 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
     4381 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
     4441 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
     4501 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     4561 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     4621 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     4681 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     4741 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     4801 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     4861 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     4921 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     4981 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     5041 cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     5101 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     5161 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     5221 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     5281 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     5341 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     5401 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     5461 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     5521 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     5581 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     5641 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     5701 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
     5761 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     5821 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     5881 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     5941 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     6001 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     6061 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     6121 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     6181 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     6241 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     6301 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     6361 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     6421 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     6481 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
     6541 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
     6601 tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac
     6661 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     6721 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     6781 cgggtttatt acagggacag cagagatcca gtttggttaa ttaaccatgg aattcgaacg
     6841 ctgacgtcat caacccgctc caaggaatcg cgggcccagt gtcactaggc gggaacaccc
     6901 agcgcgcgtg cgccctggca ggaagatggc tgtgagggac aggggagtgg cgccctgcaa
     6961 tatttgcatg tcgctatgtg ttctgggaaa tcaccataaa cgtgaaatgt ctttggattt
     7021 gggaatctta taagtcccta tcagtgatag agattcccgc acaggagctg cggcggatgt
     7081 tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg
     7141 caccgagtcg gtgctttttt tttaattaac ccgtgtcggc tccagatctg gcctccgcgc
     7201 cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac
     7261 gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc tcaggacagc ggcccgctgc
     7321 tcataagact cggccttaga accccagtat cagcagaagg acattttagg acgggacttg
     7381 ggtgactcta gggcactggt tttctttcca gagagcggaa caggcgagga aaagtagtcc
     7441 cttctcggcg attctgcgga gggatctccg tggggcggtg aacgccgatg attatataag
     7501 gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg gatttgggtc gcggttcttg
     7561 tttgtggatc gctgtgatcg tcacttggtg agtagcgggc tgctgggctg gccggggctt
     7621 tcgtggccgc cgggccgctc ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta
     7681 gtctgggtcc gcgagcaagg ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg
     7741 cggctgttcc cgagtcttga atggaagacg cttgtgaggc gggctgtgag gtcgttgaaa
     7801 caaggtgggg ggcatggtgg gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg
     7861 gaaagctctt attcgggtga gatgggctgg ggcaccatct ggggaccctg acgtgaagtt
     7921 tgtcactgac tggagaactc ggtttgtcgt ctgttgcggg ggcggcagtt atggcggtgc
     7981 cgttgggcag tgcacccgta cctttgggag cgcgcgccct cgtcgtgtcg tgacgtcacc
     8041 cgttctgttg gcttataatg cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt
     8101 tctccgtcgc aggacgcagg gttcgggcct agggtaggct ctcctgaatc gacaggcgcc
     8161 ggacctctgg tgaggggagg gataagtgag gcgtcagttt ctttggtcgg ttttatgtac
     8221 ctatcttctt aagtagctga agctccggtt ttgaactatg cgctcggggt tggcgagtgt
     8281 gttttgtgaa gttttttagg caccttttga aatgtaatca tttgggtcaa tatgtaattt
     8341 tcagtgttag actagtaaat tgtccgctaa attctggccg tttttggctt ttttgttaga
     8401 cgaagcttgg gctgcaggtc gactctagag gatccaccat gtccagactg gacaagagca
     8461 aagtcatcaa ctctgccttg gagctcctga atgaagttgg cattgagggc ttgaccacca
     8521 ggaagctggc ccagaagctg ggtgtggagc agcctaccct gtactggcat gtgaagaaca
     8581 agagggctct gcttgatgcc ctggccattg agatgttgga caggcaccac acccacttct
     8641 gccctctgga aggggagtcc tggcaggact tcctgaggaa caatgccaag agcttcagat
     8701 gtgccttgct ctcccaccgg gatggtgcca aagttcactt gggcaccagg cctacagaga
     8761 agcagtatga gaccctggag aaccagctgg cattcctgtg ccaacaaggc ttctccctgg
     8821 aaaatgcctt gtatgccctc tctgctgtgg gccacttcac cttgggctgt gtgctggagg
     8881 accaggagca ccaagttgcc aaggaggaga gggagacccc caccactgac tccatgccac
     8941 cactgctgcg gcaagctatt gagttgtttg accaccaagg ggctgagcct gcattccttt
     9001 ttggcctgga actgatcatc tgtggcctgg aaaagcagct gaaatgtgag tctggctctg
     9061 ggtccggtga gggcagagga agtctgctaa catgcggtga cgtcgaggag aatcctggcc
     9121 caatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     9181 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     9241 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     9301 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     9361 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     9421 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     9481 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     9541 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     9601 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     9661 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     9721 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     9781 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     9841 aaagcggccg cgatctacaa ttcgatatca agcttatcga taatcaacct ctggattaca
     9901 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     9961 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
    10021 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
    10081 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
    10141 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
    10201 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
    10261 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
    10321 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
    10381 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
    10441 gtcggatctc cctttgggcc gcctccccgc atcgagacct agaaaaacat ggagcaatca
    10501 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
    10561 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
    10621 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
    10681 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
    10741 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca
    10801 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct
    10861 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga
    10921 ggtttgacag ccgcctagca tttcatcaca tg
//
