LOCUS       sequence-169874-       10952 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of guide RNA (huMcl-1.2) with fluorescent GFP 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10952)
  AUTHORS   Aubrey BJ, Kelly GL, Kueh AJ, Brennan MS, O'Connor L, Milla L, 
            Wilcox S, Tai L, Strasser A, Herold MJ
  TITLE     An inducible lentiviral guide RNA platform enables the 
            identification of tumor-essential genes and tumor-promoting 
            mutations in vivo.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1422-32. doi: 
            10.1016/j.celrep.2015.02.002. Epub 2015 Feb 26.
  PUBMED    25732831
REFERENCE   2  (bases 1 to 10952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.02.002"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1422-32"
COMMENT     Sequence Label: FgH1tUTG_huMcl-1.2
FEATURES             Location/Qualifiers
     source          1..10952
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     161..178
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             204..384
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    894..1482
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1000..1011
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1409..1429
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(1522..2235,2236..2238,2239..2241))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1525..2235,2236..2238,2239..2241))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1567..1588)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1916..1935
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2175..2196
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(2242..2295)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(2305..2925)
                     /codon_start=1
                     /gene="synthetic gene"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     promoter        complement(2963..4174)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(3107..3126)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_RNA        complement(4210..4285)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    4311..4329
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(4374..4393)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_feature    4584..4701
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4999..5043)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5228..5461
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5954..6079
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6126..6306
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6324..6522)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6349..6369)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6524..6903
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     7075..7094
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7169..7273
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7274..7342,7343..8134)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7492..7511)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8305..8893
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8794..8813
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9047..9064
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9181..9202
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9217..9222,9223..9240,9241..9247)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9255..9271
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9260..9282
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9279..9295
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9279..9295
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    9344..9465
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9355..9374)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9409..9428
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(9595..9969)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(9988..10035)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(10083..10412)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10097..10232
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10151..10170)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     10397..10417
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(10426..10854)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10536..10557)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10748..10767
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    join(10900..10952,1..172)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact cagacaatgc
       61 gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc cagggtcaag
      121 gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac agtcgaggct
      181 gatcagcggg tttaaacggg ccctgctaga gattttccac actgactaaa agggtctgag
      241 ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg
      301 caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca
      361 gatctggtct aaccagagag acccagtaca gtccggatgc agctctcggg ccatgtgatg
      421 aaatgctagg cggctgtcaa acctccactc taatacttct ctctccgggt catccatccc
      481 atgcaggctc acagggtgta acaagcgggt gttctctcct tcattggctt cttctacctt
      541 ctcttgctca actggtacta gcttgtagca ccatccaaag gtcagtggat atctgatccc
      601 tggccctggt gtgtagttct gccaatcagg gaagtagcct tgtgtgtggt agatccacag
      661 atcaaggata tcttgtcttc gttgggagtg aattagccct tccagtcccc ccttttcttt
      721 taaaaagtgg ctaagatcta cagctgcctt gtaagtcatt ggtcttaaag gtacctgagg
      781 tgtgactgga aaacccacct cctcctcctc ttgtgcttct agccaggcac aatcagcatt
      841 ggtagctgct gtattgctac ttgtgattgc tccatgtttt tctaggtctc gatgcgggga
      901 ggcggcccaa agggagatcc gactcgtctg agggcgaagg cgaagacgcg gaagaggccg
      961 cagagccggc agcaggccgc gggaaggaag gtccgctgga ttgagggccg aagggacgta
     1021 gcagaaggac gtcccgcgca gaatccaggt ggcaacacag gcgagcagcc aaggaaagga
     1081 cgatgatttc cccgacaaca ccacggaatt gtcagtgccc aacagccgag cccctgtcca
     1141 gcagcgggca aggcaggcgg cgatgagttc cgccgtggca atagggaggg ggaaagcgaa
     1201 agtcccggaa aggagctgac aggtggtggc aatgccccaa ccagtggggg ttgcgtcagc
     1261 aaacacagtg cacaccacgc cacgttgcct gacaacgggc cacaactcct cataaagaga
     1321 cagcaaccag gatttataca aggaggagaa aatgaaagcc atacgggaag caatagcatg
     1381 atacaaaggc attaaagcag cgtatccaca tagcgtaaaa ggagcaacat agttaagaat
     1441 accagtcaat ctttcacaaa ttttgtaatc cagaggttga ttatcgataa gcttgatatc
     1501 gaattgtaga tcgcggccgc tttacttgta cagctcgtcc atgccgagag tgatcccggc
     1561 ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag
     1621 ggcggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg
     1681 ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat
     1741 cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct
     1801 gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc
     1861 cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt
     1921 gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc
     1981 ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc actgcacgcc
     2041 gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat
     2101 gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa
     2161 cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag
     2221 ctcctcgccc ttgctcacca ttgggccagg attctcctcg acgtcaccgc atgttagcag
     2281 acttcctctg ccctcaccgg acccagagcc agactcacat ttcagctgct tttccaggcc
     2341 acagatgatc agttccaggc caaaaaggaa tgcaggctca gccccttggt ggtcaaacaa
     2401 ctcaatagct tgccgcagca gtggtggcat ggagtcagtg gtgggggtct ccctctcctc
     2461 cttggcaact tggtgctcct ggtcctccag cacacagccc aaggtgaagt ggcccacagc
     2521 agagagggca tacaaggcat tttccaggga gaagccttgt tggcacagga atgccagctg
     2581 gttctccagg gtctcatact gcttctctgt aggcctggtg cccaagtgaa ctttggcacc
     2641 atcccggtgg gagagcaagg cacatctgaa gctcttggca ttgttcctca ggaagtcctg
     2701 ccaggactcc ccttccagag ggcagaagtg ggtgtggtgc ctgtccaaca tctcaatggc
     2761 cagggcatca agcagagccc tcttgttctt cacatgccag tacagggtag gctgctccac
     2821 acccagcttc tgggccagct tcctggtggt caagccctca atgccaactt cattcaggag
     2881 ctccaaggca gagttgatga ctttgctctt gtccagtctg gacatggtgg atcctctaga
     2941 gtcgacctgc agcccaagct tcgtctaaca aaaaagccaa aaacggccag aatttagcgg
     3001 acaatttact agtctaacac tgaaaattac atattgaccc aaatgattac atttcaaaag
     3061 gtgcctaaaa aacttcacaa aacacactcg ccaaccccga gcgcatagtt caaaaccgga
     3121 gcttcagcta cttaagaaga taggtacata aaaccgacca aagaaactga cgcctcactt
     3181 atccctcccc tcaccagagg tccggcgcct gtcgattcag gagagcctac cctaggcccg
     3241 aaccctgcgt cctgcgacgg agaaaagcct accgcacacc taccggcagg tggccccacc
     3301 ctgcattata agccaacaga acgggtgacg tcacgacacg acgagggcgc gcgctcccaa
     3361 aggtacgggt gcactgccca acggcaccgc cataactgcc gcccccgcaa cagacgacaa
     3421 accgagttct ccagtcagtg acaaacttca cgtcagggtc cccagatggt gccccagccc
     3481 atctcacccg aataagagct ttcccgcatt agcgaaggcc tcaagacctt gggttcttgc
     3541 cgcccaccat gccccccacc ttgtttcaac gacctcacag cccgcctcac aagcgtcttc
     3601 cattcaagac tcgggaacag ccgccatttt gctgcgctcc ccccaacccc cagttcaggg
     3661 caaccttgct cgcggaccca gactacagcc cttggcggtc tctccacacg cttccgtccc
     3721 accgagcggc ccggcggcca cgaaagcccc ggccagccca gcagcccgct actcaccaag
     3781 tgacgatcac agcgatccac aaacaagaac cgcgacccaa atcccggctg cgacggaact
     3841 agctgtgcca cacccggcgc gtccttatat aatcatcggc gttcaccgcc ccacggagat
     3901 ccctccgcag aatcgccgag aagggactac ttttcctcgc ctgttccgct ctctggaaag
     3961 aaaaccagtg ccctagagtc acccaagtcc cgtcctaaaa tgtccttctg ctgatactgg
     4021 ggttctaagg ccgagtctta tgagcagcgg gccgctgtcc tgagcgtccg ggcggaagga
     4081 tcaggacgct cgctgcgccc ttcgtctgac gtggcagcgc tcgccgtgag gaggggggcg
     4141 cccgcgggag gcgccaaaac ccggcgcgga ggccagatct ggagccgaca cgggttaatt
     4201 aaaaaaaaag caccgactcg gtgccacttt ttcaagttga taacggacta gccttatttt
     4261 aacttgctat ttctagctct aaaactagcc aaaagtcgcc ctcccgggaa tctctatcac
     4321 tgatagggac ttataagatt cccaaatcca aagacatttc acgtttatgg tgatttccca
     4381 gaacacatag cgacatgcaa atattgcagg gcgccactcc cctgtccctc acagccatct
     4441 tcctgccagg gcgcacgcgc gctgggtgtt cccgcctagt gacactgggc ccgcgattcc
     4501 ttggagcggg ttgatgacgt cagcgttcga attccatggt taattaacca aactggatct
     4561 ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg taatttgttt ttgtaattct
     4621 ttagtttgta tgtctgttgc tattatgtct actattcttt cccctgcact gtacccccca
     4681 atcccccctt ttcttttaaa attgtggatg aatactgcca tttgtctgca gaattggcgc
     4741 acgcagtgcc gatccgttca ctaatcgaat ggatctgtct ctgtctctct ctccaccttc
     4801 ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt gggaggtggg tctgaaacga
     4861 taatggtgaa tatccctgcc taactctatt cactatagaa agtacagcaa aaactattct
     4921 taaacctacc aagcctccta ctatcattat gaataatttt atataccaca gccaatttgt
     4981 tatgttaaac caattccaca aacttgccca tttatctaat tccaataatt cttgttcatt
     5041 cttttcttgc tggttttgcg attcttcaat taaggagtgt attaagcttg tgtaattgtt
     5101 aatttctctg tcccactcca tccaggtcgt gtgattccaa atctgttcca gagatttatt
     5161 actccaacta gcattccaag gcacagcagt ggtgcaaatg agttttccag agcaacccca
     5221 aatccccagg agctgttgat cctttaggta tctttccaca gccaggattc ttgcctggag
     5281 ctgcttgatg ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa tagccctcag
     5341 caaattgttc tgctgctgca ctataccaga caataattgt ctggcctgta ccgtcagcgt
     5401 cattgacgct gcgcccatag tgcttcctgc tgctcccaag aacccaagga acaaagctcc
     5461 tattcccact gctctttttt ctctctgcac cactcttctc tttgccttgg tgggtgctac
     5521 tcctaatggt tcaattttta ctactttata tttatataat tcacttctcc aattgtccct
     5581 catatctcct cctccaggtc tgaagatcag cggccgcttg ctgtgcggtg gtcttacttt
     5641 tgttttgctc ttcctctatc ttgtctaaag cttccttggt gtcttttatc tctatccttt
     5701 gatgcacaca atagagggtt gctactgtat tatataatga tctaagttct tctgatcctg
     5761 tctgaaggga tggttgtagc tgtcccagta tttgtctaca gccttctgat gtttctaaca
     5821 ggccaggatt aactgcgaat cgttctagct ccctgcttgc ccatactata tgttttaatt
     5881 tatatttttt ctttccccct ggccttaacc gaattttttc ccatcgcgat ctaattctcc
     5941 cccgcttaat actgacgctc tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa
     6001 tttttggcgt actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc
     6061 gagtcctgcg tcgagagagc tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc
     6121 gccactgcta gagattttcc acactgacta aaagggtctg agggatctct agttaccaga
     6181 gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt
     6241 aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag
     6301 agacccagta caggcaaaac gcgctgctta tatagacctc ccaccgtaca cgcctaccgc
     6361 ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt
     6421 gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa
     6481 accgctatcc acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga
     6541 ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat
     6601 gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca
     6661 cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga
     6721 aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt
     6781 cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat
     6841 atgggctatg aactaatgac cccgtaattg attactatta ataactagtc aataatcaat
     6901 gtcaacgcgt atatctggcc cgtacatcgc gaagcagcgc aaaacgccta accctaagca
     6961 gattcttcat gcaattgtcg gtcaagcctt gccttgttgt agcttaaatt ttgctcgcgc
     7021 actactcagc gacctccaac acacaagcag ggagcagata ctggcttaac tatgcggcat
     7081 cagagcagat tgtactgaga gtgcaccata ggggatcggg agatctcccg atccgtcgac
     7141 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     7201 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     7261 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     7321 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     7381 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     7441 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     7501 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     7561 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     7621 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     7681 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     7741 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     7801 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     7861 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     7921 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     7981 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     8041 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     8101 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     8161 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     8221 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     8281 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     8341 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     8401 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     8461 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     8521 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     8581 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     8641 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     8701 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     8761 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     8821 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     8881 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     8941 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     9001 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     9061 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     9121 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     9181 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     9241 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     9301 ttacgccaag ctctagctag aggtcgacgg tatacagaca tgataagata cattgatgag
     9361 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     9421 gctattgctt tatttgtaac cattataagc tgcaataaac aagttggggt gggcgaagaa
     9481 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc
     9541 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtagca cgtgtcagtc
     9601 ctgctcctcg gccacgaagt gcacgcagtt gccggccggg tcgcgcaggg cgaactcccg
     9661 cccccacggc tgctcgccga tctcggtcat ggccggcccg gaggcgtccc ggaagttcgt
     9721 ggacacgacc tccgaccact cggcgtacag ctcgtccagg ccgcgcaccc acacccaggc
     9781 cagggtgttg tccggcacca cctggtcctg gaccgcgctg atgaacaggg tcacgtcgtc
     9841 ccggaccaca ccggcgaagt cgtcctccac gaagtcccgg gagaacccga gccggtcggt
     9901 ccagaactcg accgctccgg cgacgtcgcg cgcggtgagc accggaacgg cactggtcaa
     9961 cttggccatg gtttagttcc tcaccttgtc gtattatact atgccgatat actatgccga
    10021 tgattaattg tcaacacgtg ctgatcagat ccgaaaatgg atatacaagc tcccgggagc
    10081 tttttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag
    10141 gcagaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa
    10201 tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt
    10261 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
    10321 ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag
    10381 cctggggact ttccacaccc taactgacac acattccaca gaattaattc gcgttaaatt
    10441 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
    10501 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
    10561 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
    10621 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
    10681 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
    10741 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
    10801 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtggggat
    10861 accccctaga gccccagctg gttctttccg cctcagaagc catagagccc accgcatccc
    10921 cagcatgcct gctattgtct tcccaatcct cc
//
