LOCUS       sequence-169876-       10952 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of guide RNA (huBim_Ex3) with fluorescent GFP 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10952)
  AUTHORS   Aubrey BJ, Kelly GL, Kueh AJ, Brennan MS, O'Connor L, Milla L, 
            Wilcox S, Tai L, Strasser A, Herold MJ
  TITLE     An inducible lentiviral guide RNA platform enables the 
            identification of tumor-essential genes and tumor-promoting 
            mutations in vivo.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1422-32. doi: 
            10.1016/j.celrep.2015.02.002. Epub 2015 Feb 26.
  PUBMED    25732831
REFERENCE   2  (bases 1 to 10952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.02.002"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1422-32"
COMMENT     Sequence Label: FgH1tUTG_huBim_Ex3
FEATURES             Location/Qualifiers
     source          1..10952
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             6..186
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(212..229)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    218..442
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      488..916
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(575..594)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     785..806
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(925..945)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        930..1259
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1110..1245
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1172..1191
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        1307..1354
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1373..1747
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1877..1998
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1914..1933)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1968..1987
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2047..2063)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2047..2063)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2060..2082)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2071..2087
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2095..2101,2102..2119,2120..2125))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2140..2161
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2278..2295)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2449..3037)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2529..2548)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3208..3999,4000..4068))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3831..3850
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4069..4173)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4248..4267)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4439..4818
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4820..5018
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4973..4993
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             5036..5216
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5263..5388
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5881..6114
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6299..6343
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6641..6758
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     6949..6968
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    7013..7031
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_RNA        7057..7132
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        7168..8379
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     8216..8235
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             8417..9037
                     /codon_start=1
                     /gene="synthetic gene"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     CDS             9047..9100
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(9101..9103,9104..9106,9107..9820)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9101..9103,9104..9106,9107..9817)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9146..9167)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9407..9426)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9754..9775
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    9860..10448
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9913..9933)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10331..10342)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
       61 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
      121 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
      181 ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
      241 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
      301 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
      361 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
      421 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
      481 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
      541 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
      601 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
      661 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
      721 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
      781 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
      841 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
      901 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      961 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     1021 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     1081 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     1141 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     1201 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     1261 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc
     1321 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa
     1381 gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg
     1441 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg
     1501 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct
     1561 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc
     1621 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg
     1681 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca
     1741 ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     1801 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     1861 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     1921 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     1981 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     2041 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     2101 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     2161 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     2221 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     2281 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     2341 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     2401 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     2461 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     2521 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     2581 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     2641 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     2701 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     2761 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     2821 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     2881 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     2941 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3001 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3061 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     3121 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     3181 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     3241 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     3301 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     3361 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     3421 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     3481 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     3541 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     3601 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     3661 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     3721 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     3781 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     3841 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     3901 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     3961 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     4021 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     4081 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     4141 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     4201 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
     4261 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
     4321 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     4381 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
     4441 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     4501 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     4561 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     4621 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     4681 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     4741 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     4801 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     4861 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     4921 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     4981 ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc
     5041 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     5101 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     5161 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     5221 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     5281 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     5341 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     5401 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     5461 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     5521 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     5581 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     5641 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     5701 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     5761 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     5821 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     5881 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     5941 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     6001 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     6061 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     6121 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     6181 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     6241 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     6301 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     6361 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     6421 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     6481 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     6541 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg
     6601 tgcgccaatt ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     6661 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     6721 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     6781 gagatccagt ttggttaatt aaccatggaa ttcgaacgct gacgtcatca acccgctcca
     6841 aggaatcgcg ggcccagtgt cactaggcgg gaacacccag cgcgcgtgcg ccctggcagg
     6901 aagatggctg tgagggacag gggagtggcg ccctgcaata tttgcatgtc gctatgtgtt
     6961 ctgggaaatc accataaacg tgaaatgtct ttggatttgg gaatcttata agtccctatc
     7021 agtgatagag attcccgccc aagagttgcg gcgtatgttt tagagctaga aatagcaagt
     7081 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttt
     7141 taattaaccc gtgtcggctc cagatctggc ctccgcgccg ggttttggcg cctcccgcgg
     7201 gcgcccccct cctcacggcg agcgctgcca cgtcagacga agggcgcagc gagcgtcctg
     7261 atccttccgc ccggacgctc aggacagcgg cccgctgctc ataagactcg gccttagaac
     7321 cccagtatca gcagaaggac attttaggac gggacttggg tgactctagg gcactggttt
     7381 tctttccaga gagcggaaca ggcgaggaaa agtagtccct tctcggcgat tctgcggagg
     7441 gatctccgtg gggcggtgaa cgccgatgat tatataagga cgcgccgggt gtggcacagc
     7501 tagttccgtc gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc
     7561 acttggtgag tagcgggctg ctgggctggc cggggctttc gtggccgccg ggccgctcgg
     7621 tgggacggaa gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc gagcaaggtt
     7681 gccctgaact gggggttggg gggagcgcag caaaatggcg gctgttcccg agtcttgaat
     7741 ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg catggtgggc
     7801 ggcaagaacc caaggtcttg aggccttcgc taatgcggga aagctcttat tcgggtgaga
     7861 tgggctgggg caccatctgg ggaccctgac gtgaagtttg tcactgactg gagaactcgg
     7921 tttgtcgtct gttgcggggg cggcagttat ggcggtgccg ttgggcagtg cacccgtacc
     7981 tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca
     8041 gggtggggcc acctgccggt aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt
     8101 tcgggcctag ggtaggctct cctgaatcga caggcgccgg acctctggtg aggggaggga
     8161 taagtgaggc gtcagtttct ttggtcggtt ttatgtacct atcttcttaa gtagctgaag
     8221 ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca
     8281 ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc agtgttagac tagtaaattg
     8341 tccgctaaat tctggccgtt tttggctttt ttgttagacg aagcttgggc tgcaggtcga
     8401 ctctagagga tccaccatgt ccagactgga caagagcaaa gtcatcaact ctgccttgga
     8461 gctcctgaat gaagttggca ttgagggctt gaccaccagg aagctggccc agaagctggg
     8521 tgtggagcag cctaccctgt actggcatgt gaagaacaag agggctctgc ttgatgccct
     8581 ggccattgag atgttggaca ggcaccacac ccacttctgc cctctggaag gggagtcctg
     8641 gcaggacttc ctgaggaaca atgccaagag cttcagatgt gccttgctct cccaccggga
     8701 tggtgccaaa gttcacttgg gcaccaggcc tacagagaag cagtatgaga ccctggagaa
     8761 ccagctggca ttcctgtgcc aacaaggctt ctccctggaa aatgccttgt atgccctctc
     8821 tgctgtgggc cacttcacct tgggctgtgt gctggaggac caggagcacc aagttgccaa
     8881 ggaggagagg gagaccccca ccactgactc catgccacca ctgctgcggc aagctattga
     8941 gttgtttgac caccaagggg ctgagcctgc attccttttt ggcctggaac tgatcatctg
     9001 tggcctggaa aagcagctga aatgtgagtc tggctctggg tccggtgagg gcagaggaag
     9061 tctgctaaca tgcggtgacg tcgaggagaa tcctggccca atggtgagca agggcgagga
     9121 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     9181 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     9241 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     9301 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     9361 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     9421 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     9481 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     9541 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     9601 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     9661 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     9721 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     9781 cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg atctacaatt
     9841 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     9901 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     9961 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
    10021 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
    10081 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
    10141 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
    10201 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
    10261 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
    10321 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
    10381 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
    10441 ctccccgcat cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
    10501 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
    10561 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
    10621 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
    10681 tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc
    10741 agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga
    10801 gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca
    10861 tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt
    10921 tcatcacatg gcccgagagc tgcatccgga ct
//
