LOCUS       sequence-169887-       10952 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of guide RNA (Bfl-1.1) with fluorescent GFP 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10952)
  AUTHORS   Herold
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: FgH1tUTG_Bfl-1.1
FEATURES             Location/Qualifiers
     source          1..10952
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(204..251)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(299..628)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      313..448
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(367..386)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     613..633
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(642..1070)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(752..773)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     964..983
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    1116..1340
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1329..1346
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             1372..1552
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2062..2650
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2168..2179
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2577..2597
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(2690..3403,3404..3406,3407..3409))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2693..3403,3404..3406,3407..3409))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2735..2756)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3084..3103
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3343..3364
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(3410..3463)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(3473..4093)
                     /codon_start=1
                     /gene="synthetic gene"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     promoter        complement(4131..5342)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(4275..4294)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_RNA        complement(5378..5453)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    5479..5497
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(5542..5561)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_feature    5752..5869
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6167..6211)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6396..6629
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7122..7247
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7294..7474
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7492..7690)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7517..7537)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7692..8071
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     8243..8262
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8337..8441
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8442..8510,8511..9302)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8660..8679)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9473..10061
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9962..9981
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10215..10232
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10349..10370
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10385..10390,10391..10408,10409..10415)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10423..10439
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10428..10450
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10447..10463
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10447..10463
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    10512..10633
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10523..10542)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10577..10596
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(10763..10952,1..185))
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
ORIGIN
        1 gtgttgtccg gcaccacctg gtcctggacc gcgctgatga acagggtcac gtcgtcccgg
       61 accacaccgg cgaagtcgtc ctccacgaag tcccgggaga acccgagccg gtcggtccag
      121 aactcgaccg ctccggcgac gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg
      181 gccatggttt agttcctcac cttgtcgtat tatactatgc cgatatacta tgccgatgat
      241 taattgtcaa cacgtgctga tcagatccga aaatggatat acaagctccc gggagctttt
      301 tgcaaaagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggcag
      361 aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg
      421 cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg
      481 actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
      541 acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg
      601 gggactttcc acaccctaac tgacacacat tccacagaat taattcgcgt taaatttttg
      661 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
      721 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
      781 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
      841 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
      901 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
      961 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     1021 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc
     1081 cctagagccc cagctggttc tttccgcctc agaagccata gagcccaccg catccccagc
     1141 atgcctgcta ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc caccccccag
     1201 aatagaatga cacctactca gacaatgcga tgcaatttcc tcattttatt aggaaaggac
     1261 agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg
     1321 ctggcaacta gaaggcacag tcgaggctga tcagcgggtt taaacgggcc ctgctagaga
     1381 ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac
     1441 gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc
     1501 ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac ccagtacagt
     1561 ccggatgcag ctctcgggcc atgtgatgaa atgctaggcg gctgtcaaac ctccactcta
     1621 atacttctct ctccgggtca tccatcccat gcaggctcac agggtgtaac aagcgggtgt
     1681 tctctccttc attggcttct tctaccttct cttgctcaac tggtactagc ttgtagcacc
     1741 atccaaaggt cagtggatat ctgatccctg gccctggtgt gtagttctgc caatcaggga
     1801 agtagccttg tgtgtggtag atccacagat caaggatatc ttgtcttcgt tgggagtgaa
     1861 ttagcccttc cagtcccccc ttttctttta aaaagtggct aagatctaca gctgccttgt
     1921 aagtcattgg tcttaaaggt acctgaggtg tgactggaaa acccacctcc tcctcctctt
     1981 gtgcttctag ccaggcacaa tcagcattgg tagctgctgt attgctactt gtgattgctc
     2041 catgtttttc taggtctcga tgcggggagg cggcccaaag ggagatccga ctcgtctgag
     2101 ggcgaaggcg aagacgcgga agaggccgca gagccggcag caggccgcgg gaaggaaggt
     2161 ccgctggatt gagggccgaa gggacgtagc agaaggacgt cccgcgcaga atccaggtgg
     2221 caacacaggc gagcagccaa ggaaaggacg atgatttccc cgacaacacc acggaattgt
     2281 cagtgcccaa cagccgagcc cctgtccagc agcgggcaag gcaggcggcg atgagttccg
     2341 ccgtggcaat agggaggggg aaagcgaaag tcccggaaag gagctgacag gtggtggcaa
     2401 tgccccaacc agtgggggtt gcgtcagcaa acacagtgca caccacgcca cgttgcctga
     2461 caacgggcca caactcctca taaagagaca gcaaccagga tttatacaag gaggagaaaa
     2521 tgaaagccat acgggaagca atagcatgat acaaaggcat taaagcagcg tatccacata
     2581 gcgtaaaagg agcaacatag ttaagaatac cagtcaatct ttcacaaatt ttgtaatcca
     2641 gaggttgatt atcgataagc ttgatatcga attgtagatc gcggccgctt tacttgtaca
     2701 gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat
     2761 cgcgcttctc gttggggtct ttgctcaggg cggactgggt gctcaggtag tggttgtcgg
     2821 gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca
     2881 cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct
     2941 tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga
     3001 tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc
     3061 cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc
     3121 gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt
     3181 cggggtagcg gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg
     3241 gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat
     3301 cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga
     3361 ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatt gggccaggat
     3421 tctcctcgac gtcaccgcat gttagcagac ttcctctgcc ctcaccggac ccagagccag
     3481 actcacattt cagctgcttt tccaggccac agatgatcag ttccaggcca aaaaggaatg
     3541 caggctcagc cccttggtgg tcaaacaact caatagcttg ccgcagcagt ggtggcatgg
     3601 agtcagtggt gggggtctcc ctctcctcct tggcaacttg gtgctcctgg tcctccagca
     3661 cacagcccaa ggtgaagtgg cccacagcag agagggcata caaggcattt tccagggaga
     3721 agccttgttg gcacaggaat gccagctggt tctccagggt ctcatactgc ttctctgtag
     3781 gcctggtgcc caagtgaact ttggcaccat cccggtggga gagcaaggca catctgaagc
     3841 tcttggcatt gttcctcagg aagtcctgcc aggactcccc ttccagaggg cagaagtggg
     3901 tgtggtgcct gtccaacatc tcaatggcca gggcatcaag cagagccctc ttgttcttca
     3961 catgccagta cagggtaggc tgctccacac ccagcttctg ggccagcttc ctggtggtca
     4021 agccctcaat gccaacttca ttcaggagct ccaaggcaga gttgatgact ttgctcttgt
     4081 ccagtctgga catggtggat cctctagagt cgacctgcag cccaagcttc gtctaacaaa
     4141 aaagccaaaa acggccagaa tttagcggac aatttactag tctaacactg aaaattacat
     4201 attgacccaa atgattacat ttcaaaaggt gcctaaaaaa cttcacaaaa cacactcgcc
     4261 aaccccgagc gcatagttca aaaccggagc ttcagctact taagaagata ggtacataaa
     4321 accgaccaaa gaaactgacg cctcacttat ccctcccctc accagaggtc cggcgcctgt
     4381 cgattcagga gagcctaccc taggcccgaa ccctgcgtcc tgcgacggag aaaagcctac
     4441 cgcacaccta ccggcaggtg gccccaccct gcattataag ccaacagaac gggtgacgtc
     4501 acgacacgac gagggcgcgc gctcccaaag gtacgggtgc actgcccaac ggcaccgcca
     4561 taactgccgc ccccgcaaca gacgacaaac cgagttctcc agtcagtgac aaacttcacg
     4621 tcagggtccc cagatggtgc cccagcccat ctcacccgaa taagagcttt cccgcattag
     4681 cgaaggcctc aagaccttgg gttcttgccg cccaccatgc cccccacctt gtttcaacga
     4741 cctcacagcc cgcctcacaa gcgtcttcca ttcaagactc gggaacagcc gccattttgc
     4801 tgcgctcccc ccaaccccca gttcagggca accttgctcg cggacccaga ctacagccct
     4861 tggcggtctc tccacacgct tccgtcccac cgagcggccc ggcggccacg aaagccccgg
     4921 ccagcccagc agcccgctac tcaccaagtg acgatcacag cgatccacaa acaagaaccg
     4981 cgacccaaat cccggctgcg acggaactag ctgtgccaca cccggcgcgt ccttatataa
     5041 tcatcggcgt tcaccgcccc acggagatcc ctccgcagaa tcgccgagaa gggactactt
     5101 ttcctcgcct gttccgctct ctggaaagaa aaccagtgcc ctagagtcac ccaagtcccg
     5161 tcctaaaatg tccttctgct gatactgggg ttctaaggcc gagtcttatg agcagcgggc
     5221 cgctgtcctg agcgtccggg cggaaggatc aggacgctcg ctgcgccctt cgtctgacgt
     5281 ggcagcgctc gccgtgagga ggggggcgcc cgcgggaggc gccaaaaccc ggcgcggagg
     5341 ccagatctgg agccgacacg ggttaattaa aaaaaaagca ccgactcggt gccacttttt
     5401 caagttgata acggactagc cttattttaa cttgctattt ctagctctaa aacggttgtg
     5461 gtatctgtag gacgggaatc tctatcactg atagggactt ataagattcc caaatccaaa
     5521 gacatttcac gtttatggtg atttcccaga acacatagcg acatgcaaat attgcagggc
     5581 gccactcccc tgtccctcac agccatcttc ctgccagggc gcacgcgcgc tgggtgttcc
     5641 cgcctagtga cactgggccc gcgattcctt ggagcgggtt gatgacgtca gcgttcgaat
     5701 tccatggtta attaaccaaa ctggatctct gctgtccctg taataaaccc gaaaattttg
     5761 aatttttgta atttgttttt gtaattcttt agtttgtatg tctgttgcta ttatgtctac
     5821 tattctttcc cctgcactgt accccccaat cccccctttt cttttaaaat tgtggatgaa
     5881 tactgccatt tgtctgcaga attggcgcac gcagtgccga tccgttcact aatcgaatgg
     5941 atctgtctct gtctctctct ccaccttctt cttctattcc ttcgggcctg tcgggtcccc
     6001 tcggggttgg gaggtgggtc tgaaacgata atggtgaata tccctgccta actctattca
     6061 ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact atcattatga
     6121 ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa cttgcccatt
     6181 tatctaattc caataattct tgttcattct tttcttgctg gttttgcgat tcttcaatta
     6241 aggagtgtat taagcttgtg taattgttaa tttctctgtc ccactccatc caggtcgtgt
     6301 gattccaaat ctgttccaga gatttattac tccaactagc attccaaggc acagcagtgg
     6361 tgcaaatgag ttttccagag caaccccaaa tccccaggag ctgttgatcc tttaggtatc
     6421 tttccacagc caggattctt gcctggagct gcttgatgcc ccagactgtg agttgcaaca
     6481 gatgctgttg cgcctcaata gccctcagca aattgttctg ctgctgcact ataccagaca
     6541 ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg
     6601 ctcccaagaa cccaaggaac aaagctccta ttcccactgc tcttttttct ctctgcacca
     6661 ctcttctctt tgccttggtg ggtgctactc ctaatggttc aatttttact actttatatt
     6721 tatataattc acttctccaa ttgtccctca tatctcctcc tccaggtctg aagatcagcg
     6781 gccgcttgct gtgcggtggt cttacttttg ttttgctctt cctctatctt gtctaaagct
     6841 tccttggtgt cttttatctc tatcctttga tgcacacaat agagggttgc tactgtatta
     6901 tataatgatc taagttcttc tgatcctgtc tgaagggatg gttgtagctg tcccagtatt
     6961 tgtctacagc cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg ttctagctcc
     7021 ctgcttgccc atactatatg ttttaattta tattttttct ttccccctgg ccttaaccga
     7081 attttttccc atcgcgatct aattctcccc cgcttaatac tgacgctctc gcacccatct
     7141 ctctccttct agcctccgct agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg
     7201 cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc ctctggtttc
     7261 cctttcgctt tcaagtccct gttcgggcgc cactgctaga gattttccac actgactaaa
     7321 agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa
     7381 gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct
     7441 cccaggctca gatctggtct aaccagagag acccagtaca ggcaaaacgc gctgcttata
     7501 tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga
     7561 cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg
     7621 tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa
     7681 aaccgcatca ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag
     7741 tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca
     7801 atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt
     7861 aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac
     7921 gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc
     7981 gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat
     8041 tactattaat aactagtcaa taatcaatgt caacgcgtat atctggcccg tacatcgcga
     8101 agcagcgcaa aacgcctaac cctaagcaga ttcttcatgc aattgtcggt caagccttgc
     8161 cttgttgtag cttaaatttt gctcgcgcac tactcagcga cctccaacac acaagcaggg
     8221 agcagatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatagg
     8281 ggatcgggag atctcccgat ccgtcgacgt caggtggcac ttttcgggga aatgtgcgcg
     8341 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     8401 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     8461 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     8521 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     8581 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     8641 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     8701 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     8761 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     8821 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     8881 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     8941 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     9001 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     9061 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     9121 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     9181 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     9241 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     9301 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     9361 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     9421 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     9481 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     9541 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     9601 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     9661 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     9721 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     9781 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     9841 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     9901 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     9961 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    10021 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    10081 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    10141 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    10201 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
    10261 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
    10321 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
    10381 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    10441 ttcacacagg aaacagctat gaccatgatt acgccaagct ctagctagag gtcgacggta
    10501 tacagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
    10561 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
    10621 caataaacaa gttggggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca
    10681 tccagccggc gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg
    10741 aatcgaaatc tcgtagcacg tgtcagtcct gctcctcggc cacgaagtgc acgcagttgc
    10801 cggccgggtc gcgcagggcg aactcccgcc cccacggctg ctcgccgatc tcggtcatgg
    10861 ccggcccgga ggcgtcccgg aagttcgtgg acacgacctc cgaccactcg gcgtacagct
    10921 cgtccaggcc gcgcacccac acccaggcca gg
//
