LOCUS       sequence-169900-        8956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral Gateway-compatible expression vector backbone with a 
            C-terminal 3xFLAG-V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8956)
  AUTHORS   Taipale M, Tucker G, Peng J, Krykbaeva I, Lin ZY, Larsen B, Choi H, 
            Berger B, Gingras AC, Lindquist S
  TITLE     A quantitative chaperone interaction network reveals the 
            architecture of cellular protein homeostasis pathways.
  JOURNAL   Cell. 2014 Jul 17;158(2):434-48. doi: 10.1016/j.cell.2014.05.039.
  PUBMED    25036637
REFERENCE   2  (bases 1 to 8956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.05"; journalName: "Cell"; date: "2014-07-17- 
            17"; volume: "158"; issue: "2"; pages: "434-48"
COMMENT     Sequence Label: pLenti6.2-ccdB-3xFLAG-V5
FEATURES             Location/Qualifiers
     source          1..8956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        1816..2119
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2120..2323
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2274..2294
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        2368..2386
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2474..2598
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2623..2628,2629..2646,2647..2653)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2707..3366
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2773..2792)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3708..4013
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3892..3911
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4054..4178)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4180..4245
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4264..4305
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4340..4381
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        4414..4912
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4449..4472)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4845..4864
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        4926..4973
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4992..5390
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5477..5710
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5782..5903
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5819..5838)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5873..5892
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5943..6078
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6099..6117)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6127..6144)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6127..6143)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6136..6158)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6285..6740
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6372..6391)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6582..6603
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6766..6870
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6871..6939,6940..7731)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7089..7108)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7902..8490
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8391..8410
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8644..8661
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8778..8799
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8814..8819,8820..8837,8838..8844)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8852..8868
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8857..8879
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8876..8892
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8876..8892
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8911..8931
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8913..8931
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgata
     1801 agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     1861 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     1921 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     1981 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     2041 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     2101 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     2161 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     2221 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     2281 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga
     2341 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg
     2401 ctagcgttta aacttaagct tggtaccgag ctcggatcca ctagtccagt gtggtggaat
     2461 tctgcagata tcaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa
     2521 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca
     2581 acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc
     2641 ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa
     2701 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
     2761 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
     2821 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
     2881 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa
     2941 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
     3001 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
     3061 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
     3121 attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta
     3181 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg
     3241 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc
     3301 ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg
     3361 gcgtaaagat ctggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct
     3421 gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt
     3481 atgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg
     3541 catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg
     3601 tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt
     3661 tattgaaatg aacggctctt ttgctgacga gaacaggggc tggtgaaatg cagtttaagg
     3721 tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta
     3781 ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata
     3841 aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga
     3901 ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc
     3961 accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag
     4021 gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta
     4081 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca
     4141 ttttacgttt ctcgttcagc tttcttgtac aaagtggttg attacaaaga ccatgacggt
     4201 gattataaag atcatgacat cgactacaag gatgacgatg acaagggctc gggctcgacc
     4261 tcgggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacatgata actcgagtct
     4321 agagggcccg cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac
     4381 gcgtaccggt tagtaatgac gatggtacct accgggtagg ggaggcgctt ttcccaaggc
     4441 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
     4501 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
     4561 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
     4621 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
     4681 gcaccgctga gcaatggaag cdggtaggcc tttggggcag cggccaatag cagctttgct
     4741 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
     4801 ggctcagggg cggggcgggc gcccgaagtc ctccggaggc ccggcattct gcacgcttca
     4861 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgactctaga
     4921 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     4981 ggaactaaac catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa
     5041 cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct
     5101 ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg
     5161 cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg
     5221 tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc
     5281 ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg
     5341 cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gcacaattcg
     5401 agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     5461 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     5521 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     5581 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     5641 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     5701 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     5761 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     5821 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     5881 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     5941 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     6001 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     6061 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     6121 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     6181 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     6241 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     6301 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     6361 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     6421 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     6481 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     6541 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     6601 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     6661 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     6721 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     6781 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     6841 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     6901 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     6961 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     7021 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     7081 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     7141 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     7201 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     7261 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     7321 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     7381 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     7441 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     7501 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     7561 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     7621 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     7681 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     7741 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     7801 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     7861 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7921 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7981 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     8041 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     8101 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     8161 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     8221 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     8281 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     8341 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     8401 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     8461 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     8521 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     8581 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     8641 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     8701 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     8761 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     8821 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     8881 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     8941 ctggagctgc aagctt
//
