LOCUS       sequence-169902-       10951 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of guide with fluorescent Cherry reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10951)
  AUTHORS   Herold
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: FgH1tUTCherry
FEATURES             Location/Qualifiers
     source          1..10951
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2511..2530
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    2575..2593
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_RNA        2615..2690
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2726..3937
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3774..3793
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             3981..4601
                     /codon_start=1
                     /gene="synthetic gene"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     CDS             4611..4664
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4665..5375
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4810..4828)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5078..5097
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5403..5991
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5456..5476)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5874..5885)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5994..6010)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             6519..6699
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6725..6742)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6731..6955
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7001..7429
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7088..7107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7298..7319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7438..7458)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7443..7772
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7623..7758
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7685..7704
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7820..7867
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7886..8260
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8390..8511
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8427..8446)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8481..8500
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8560..8576)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8560..8576)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8573..8595)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8584..8600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8608..8614,8615..8632,8633..8638))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8653..8674
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8791..8808)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8962..9550)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9042..9061)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9721..10512,10513..10581))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10344..10363
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10582..10686)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10761..10780)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaaccatgg aattcgaacg ctgacgtcat caacccgctc
     2401 caaggaatcg cgggcccagt gtcactaggc gggaacaccc agcgcgcgtg cgccctggca
     2461 ggaagatggc tgtgagggac aggggagtgg cgccctgcaa tatttgcatg tcgctatgtg
     2521 ttctgggaaa tcaccataaa cgtgaaatgt ctttggattt gggaatctta taagtcccta
     2581 tcagtgatag agattcccgg agacggacgt ctcggtttta gagctagaaa tagcaagtta
     2641 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttttta
     2701 attaacccgt gtcggctcca gatctggcct ccgcgccggg ttttggcgcc tcccgcgggc
     2761 gcccccctcc tcacggcgag cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat
     2821 ccttccgccc ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc
     2881 cagtatcagc agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc
     2941 tttccagaga gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga
     3001 tctccgtggg gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta
     3061 gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac
     3121 ttggtgagta gcgggctgct gggctggccg gggctttcgt ggccgccggg ccgctcggtg
     3181 ggacggaagc gtgtggagag accgccaagg gctgtagtct gggtccgcga gcaaggttgc
     3241 cctgaactgg gggttggggg gagcgcagca aaatggcggc tgttcccgag tcttgaatgg
     3301 aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg
     3361 caagaaccca aggtcttgag gccttcgcta atgcgggaaa gctcttattc gggtgagatg
     3421 ggctggggca ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcggtt
     3481 tgtcgtctgt tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca cccgtacctt
     3541 tgggagcgcg cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg
     3601 gtggggccac ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc
     3661 gggcctaggg taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata
     3721 agtgaggcgt cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct
     3781 ccggttttga actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc
     3841 ttttgaaatg taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc
     3901 cgctaaattc tggccgtttt tggctttttt gttagacgaa gcttgggctg caggtcgact
     3961 ctagaggatc tgaattcacc atgtccagac tggacaagag caaagtcatc aactctgcct
     4021 tggagctcct gaatgaagtt ggcattgagg gcttgaccac caggaagctg gcccagaagc
     4081 tgggtgtgga gcagcctacc ctgtactggc atgtgaagaa caagagggct ctgcttgatg
     4141 ccctggccat tgagatgttg gacaggcacc acacccactt ctgccctctg gaaggggagt
     4201 cctggcagga cttcctgagg aacaatgcca agagcttcag atgtgccttg ctctcccacc
     4261 gggatggtgc caaagttcac ttgggcacca ggcctacaga gaagcagtat gagaccctgg
     4321 agaaccagct ggcattcctg tgccaacaag gcttctccct ggaaaatgcc ttgtatgccc
     4381 tctctgctgt gggccacttc accttgggct gtgtgctgga ggaccaggag caccaagttg
     4441 ccaaggagga gagggagacc cccaccactg actccatgcc accactgctg cggcaagcta
     4501 ttgagttgtt tgaccaccaa ggggctgagc ctgcattcct ttttggcctg gaactgatca
     4561 tctgtggcct ggaaaagcag ctgaaatgtg agtctggctc tggatccggt gagggcagag
     4621 gaagtctgct aacatgcggt gacgtcgagg agaatcctgg cccaatggtg agcaagggcg
     4681 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
     4741 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     4801 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
     4861 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     4921 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     4981 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
     5041 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     5101 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
     5161 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
     5221 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
     5281 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
     5341 actccaccgg cggcatggac gagctgtaca agtagtgaca attcgatatc aagcttatcg
     5401 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     5461 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     5521 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     5581 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     5641 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     5701 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     5761 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     5821 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     5881 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     5941 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     6001 gtcgacctcg tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     6061 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     6121 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     6181 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg
     6241 atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc
     6301 cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag
     6361 agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc
     6421 atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat
     6481 ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga
     6541 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     6601 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     6661 ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga
     6721 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     6781 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     6841 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     6901 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     6961 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     7021 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     7081 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     7141 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     7201 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     7261 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     7321 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     7381 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     7441 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     7501 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     7561 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     7621 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     7681 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     7741 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     7801 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
     7861 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac
     7921 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
     7981 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
     8041 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
     8101 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
     8161 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
     8221 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga
     8281 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     8341 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     8401 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     8461 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     8521 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     8581 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     8641 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     8701 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     8761 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     8821 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8881 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8941 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     9001 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     9061 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     9121 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     9181 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     9241 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     9301 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     9361 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     9421 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     9481 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     9541 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     9601 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     9661 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     9721 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     9781 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9841 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9901 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9961 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    10021 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    10081 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    10141 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    10201 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    10261 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    10321 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    10381 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    10441 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    10501 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    10561 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    10621 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    10681 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
    10741 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
    10801 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
    10861 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
    10921 cttcgcgatg tacgggccag atatacgcgt t
//
