LOCUS       sequence-169918-        4655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Diehl WE, Lin AE, Grubaugh ND, Carvalho LM, Kim K, Kyawe PP, 
            McCauley SM, Donnard E, Kucukural A, McDonel P, Schaffner SF, Garber
            M, Rambaut A, Andersen KG, Sabeti PC, Luban J
  TITLE     Ebola Virus Glycoprotein with Increased Infectivity Dominated the 
            2013-2016 Epidemic.
  JOURNAL   Cell. 2016 Nov 3;167(4):1088-1098.e6. doi: 
            10.1016/j.cell.2016.10.014.
  PUBMED    27814506
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.10"; journalName: "Cell"; date: "2016-11-3- 3";
            volume: "167"; issue: "4"; pages: "1088-1098.e6"
COMMENT     Sequence Label: pGL4.23 WT 2014 EBOV Delta-Mucin-Like-Domain
FEATURES             Location/Qualifiers
     source          1..4655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    249..370
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(260..279)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     314..333
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(618..635)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(789..1377)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(869..888)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1577..2368,2369..2437))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    2542..2590
                     /note="synthetic polyadenylation signal"
     misc_feature    2604..2695
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2644..2663
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     enhancer        2725..3104
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     3259..3279
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        3313..3344
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      3371..3380
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctgtatcga accacatgat tggaccaaga acataacaga caaaattgat cagattattc
       61 atgattttgt tgataaaacc cttccggacc agggggacaa tgacaattgg tggacaggat
      121 ggagacaatg gataccggca ggtattggag ttacaggtgt tataattgca gttatcgctt
      181 tattctgtat atgcaaattt gtcttttagt ctagagtcgg ggcggccggc cgcttcgagc
      241 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
      301 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
      361 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
      421 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtc
      481 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
      541 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
      601 cagcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      661 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      721 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      781 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      841 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      901 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      961 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1021 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1081 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1141 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1201 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1261 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1321 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1381 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1441 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     1501 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagcgg ccgcaaatgc
     1561 taaaccactg cagtggttac cagtgcttga tcagtgaggc accgatctca gcgatctgcc
     1621 tatttcgttc gtccatagtg gcctgactcc ccgtcgtgta gatcactacg attcgtgagg
     1681 gcttaccatc aggccccagc gcagcaatga tgccgcgaga gccgcgttca ccggcccccg
     1741 atttgtcagc aatgaaccag ccagcaggga gggccgagcg aagaagtggt cctgctactt
     1801 tgtccgcctc catccagtct atgagctgct gtcgtgatgc tagagtaaga agttcgccag
     1861 tgagtagttt ccgaagagtt gtggccattg ctactggcat cgtggtatca cgctcgtcgt
     1921 tcggtatggc ttcgttcaac tctggttccc agcggtcaag ccgggtcaca tgatcaccca
     1981 tattatgaag aaatgcagtc agctccttag ggcctccgat cgttgtcaga agtaagttgg
     2041 ccgcggtgtt gtcgctcatg gtaatggcag cactacacaa ttctcttacc gtcatgccat
     2101 ccgtaagatg cttttccgtg accggcgagt actcaaccaa gtcgttttgt gagtagtgta
     2161 tacggcgacc aagctgctct tgcccggcgt ctatacggga caacaccgcg ccacatagca
     2221 gtactttgaa agtgctcatc atcgggaatc gttcttcggg gcggaaagac tcaaggatct
     2281 tgccgctatt gagatccagt tcgatatagc ccactcttgc acccagttga tcttcagcat
     2341 cttttacttt caccagcgtt tcggggtgtg caaaaacagg caagcaaaat gccgcaaaga
     2401 agggaatgag tgcgacacga aaatgttgga tgctcatact cgtccttttt caatattatt
     2461 gaagcattta tcagggttac tagtacgtct ctcaaggata agtaagtaat attaaggtac
     2521 gggaggtatt ggacaggccg caataaaata tctttatttt cattacatct gtgtgttggt
     2581 tttttgtgtg aatcgatagt actaacatac gctctccatc aaaacaaaac gaaacaaaac
     2641 aaactagcaa aataggctgt ccccagtgca agtgcaggtg ccagaacatt tctctggcct
     2701 aactggccgc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
     2761 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     2821 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2881 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2941 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     3001 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     3061 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     3121 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     3181 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     3241 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtggggc ctcggcggcc
     3301 aagcttagac actagagggt atataatgga agctcgactt ccagcttggc aatccggtac
     3361 tgttggtaaa gccaccatgg gtgttacagg aatattgcag ttacctcgtg atcgattcaa
     3421 gaggacatca ttctttcttt gggtaattat ccttttccaa agaacatttt ccatcccgct
     3481 tggagttatc cacaatagta cattacaggt tagtgatgtc gacaaactag tttgtcgtga
     3541 caaactgtca tccacaaatc aattgagatc agttggactg aatctcgagg ggaatggagt
     3601 ggcaactgac gtgccatctg cgactaaaag atggggcttc aggtccggtg tcccaccaaa
     3661 ggtggtcaat tatgaagctg gtgaatgggc tgaaaactgc tacaatcttg aaatcaaaaa
     3721 acctgacggg agtgagtgtc taccagcagc gccagacggg attcggggct tcccccggtg
     3781 ccggtatgtg cacaaagtat caggaacggg accatgtgcc ggagactttg ccttccacaa
     3841 agagggtgct ttcttcctgt atgatcgact tgcttccaca gttatctacc gaggaacgac
     3901 tttcgctgaa ggtgtcgttg catttctgat actgccccaa gctaagaagg acttcttcag
     3961 ctcacacccc ttgagagagc cggtcaatgc aacggaggac ccgtcgagtg gctattattc
     4021 taccacaatt agatatcagg ctaccggttt tggaactaat gagacagagt acttgttcga
     4081 ggttgacaat ttgacctacg tccaacttga atcaagattc acaccacagt ttctgctcca
     4141 gctgaatgag acaatatatg caagtgggaa gaggagcaac accacgggaa aactaatttg
     4201 gaaggtcaac cccgaaattg atacaacaat cggggagtgg gccttctggg aaactaaaaa
     4261 aaacctcact agaaaaattc gcagtgaaga gttgtctttc gcaggactga tcacaggcgg
     4321 gagaaggact cgaagagaag taattgtcaa tgctcaaccc aaatgcaacc ccaatttaca
     4381 ttactggact actcaggatg aaggtgctgc aatcggattg gcctggatac catatttcgg
     4441 gccagcagcc gaaggaattt acacagaggg gctaatgcac aaccaagatg gtttaatctg
     4501 tgggttgagg cagctggcca acgaaacgac tcaagctctc caactgttcc tgagagccac
     4561 aactgagctg cgaacctttt caatcctcaa ccgtaaggca attgacttcc tgctgcagcg
     4621 atggggtggc acatgccaca ttttgggacc ggact
//
