LOCUS       sequence-169923-        4655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Diehl WE, Lin AE, Grubaugh ND, Carvalho LM, Kim K, Kyawe PP, 
            McCauley SM, Donnard E, Kucukural A, McDonel P, Schaffner SF, Garber
            M, Rambaut A, Andersen KG, Sabeti PC, Luban J
  TITLE     Ebola Virus Glycoprotein with Increased Infectivity Dominated the 
            2013-2016 Epidemic.
  JOURNAL   Cell. 2016 Nov 3;167(4):1088-1098.e6. doi: 
            10.1016/j.cell.2016.10.014.
  PUBMED    27814506
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.10"; journalName: "Cell"; date: "2016-11-3- 3";
            volume: "167"; issue: "4"; pages: "1088-1098.e6"
COMMENT     Sequence Label: pGL4.23 A82V/T230A 2014 EBOV Delta-Mucin-Like-...
FEATURES             Location/Qualifiers
     source          1..4655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    175..296
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(212..231)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     266..285
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     regulatory      1820..1829
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1856..1887)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     primer_bind     complement(1921..1941)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2096..2475
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    2505..2596
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(2537..2556)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    2610..2658
                     /note="synthetic polyadenylation signal"
     CDS             join(2763..2831,2832..3623)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3823..4411
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4312..4331
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4565..4582
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc
       61 ggtcgacgga tccttatcga ttttaccaca tttgtagagg ttttacttgc tttaaaaaac
      121 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
      181 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
      241 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
      301 gtctgctcga agcggccggc cgccccgact ctagactaaa agacaaattt gcatatacag
      361 aataaagcga taactgcaat tataacacct gtaactccaa tacctgccgg tatccattgt
      421 ctccatcctg tccaccaatt gtcattgtcc ccctggtccg gaagggtttt atcaacaaaa
      481 tcatgaataa tctgatcaat tttgtctgtt atgttcttgg tccaatcatg tggttcgata
      541 cagcagtccg gtcccaaaat gtggcatgtg ccaccccatc gctgcagcag gaagtcaatt
      601 gccttacggt tgaggattga aaaggttcgc agctcagttg tggctctcag gaacagttgg
      661 agagcttgag tcgtttcgtt ggccagctgc ctcaacccac agattaaacc atcttggttg
      721 tgcattagcc cctctgtgta aattccttcg gctgctggcc cgaaatatgg tatccaggcc
      781 aatccgattg cagcaccttc atcctgagta gtccagtaat gtaaattggg gttgcatttg
      841 ggttgagcat tgacaattac ttctcttcga gtccttctcc cgcctgtgat cagtcctgcg
      901 aaagacaact cttcactgcg aatttttcta gtgaggtttt ttttagtttc ccagaaggcc
      961 cactccccga ttgttgtatc aatttcgggg ttgaccttcc aaattagttt tcccgtggtg
     1021 ttgctcctct tcccacttgc atatattgtc tcattcagct ggagcagaaa ctgtggtgtg
     1081 aatcttgatt caagttggac gtaggtcaaa ttgtcaacct cgaacaagta ctctgcctca
     1141 ttagttccaa aaccggtagc ctgatatcta attgtggtag aataatagcc actcgacggg
     1201 tcctccgttg cattgaccgg ctctctcaag gggtgtgagc tgaagaagtc cttcttagct
     1261 tggggcagta tcagaaatgc aacgacacct tcagcgaaag tcgttcctcg gtagataact
     1321 gtggaagcaa gtcgatcata caggaagaaa gcaccctctt tgtggaaggc aaagtctccg
     1381 gcacatggtc ccgttcctga tactttgtgc acataccggc accgggggaa gccccgaatc
     1441 ccgtctggcg ctgctggtag acactcactc ccgtcaggtt ttttgatttc aagattgtag
     1501 cagttttcag cccattcacc agcttcataa ttgaccacct ttggtgggac accggacctg
     1561 aagccccatc ttttagtcac agatggcacg tcagttgcca ctccattccc ctcgagattc
     1621 agtccaactg atctcaattg atttgtggat gacagtttgt cacgacaaac tagtttgtcg
     1681 acatcactaa cctgtaatgt actattgtgg ataactccaa gcgggatgga aaatgttctt
     1741 tggaaaagga taattaccca aagaaagaat gatgtcctct tgaatcgatc acgaggtaac
     1801 tgcaatattc ctgtaacacc catggtggct ttaccaacag taccggattg ccaagctgga
     1861 agtcgagctt ccattatata ccctctagtg tctaagcttg gccgccgagg ccccaccgta
     1921 cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc
     1981 gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc
     2041 cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg
     2101 taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt
     2161 actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg
     2221 gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt
     2281 gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca
     2341 atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc
     2401 ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat taataactag
     2461 tcaataatca atgtcaacgc gtatatctgg cggccagtta ggccagagaa atgttctggc
     2521 acctgcactt gcactgggga cagcctattt tgctagtttg ttttgtttcg ttttgttttg
     2581 atggagagcg tatgttagta ctatcgattc acacaaaaaa ccaacacaca gatgtaatga
     2641 aaataaagat attttattgc ggcctgtcca atacctcccg taccttaata ttacttactt
     2701 atccttgaga gacgtactag taaccctgat aaatgcttca ataatattga aaaaggacga
     2761 gtatgagcat ccaacatttt cgtgtcgcac tcattccctt ctttgcggca ttttgcttgc
     2821 ctgtttttgc acaccccgaa acgctggtga aagtaaaaga tgctgaagat caactgggtg
     2881 caagagtggg ctatatcgaa ctggatctca atagcggcaa gatccttgag tctttccgcc
     2941 ccgaagaacg attcccgatg atgagcactt tcaaagtact gctatgtggc gcggtgttgt
     3001 cccgtataga cgccgggcaa gagcagcttg gtcgccgtat acactactca caaaacgact
     3061 tggttgagta ctcgccggtc acggaaaagc atcttacgga tggcatgacg gtaagagaat
     3121 tgtgtagtgc tgccattacc atgagcgaca acaccgcggc caacttactt ctgacaacga
     3181 tcggaggccc taaggagctg actgcatttc ttcataatat gggtgatcat gtgacccggc
     3241 ttgaccgctg ggaaccagag ttgaacgaag ccataccgaa cgacgagcgt gataccacga
     3301 tgccagtagc aatggccaca actcttcgga aactactcac tggcgaactt cttactctag
     3361 catcacgaca gcagctcata gactggatgg aggcggacaa agtagcagga ccacttcttc
     3421 gctcggccct ccctgctggc tggttcattg ctgacaaatc gggggccggt gaacgcggct
     3481 ctcgcggcat cattgctgcg ctggggcctg atggtaagcc ctcacgaatc gtagtgatct
     3541 acacgacggg gagtcaggcc actatggacg aacgaaatag gcagatcgct gagatcggtg
     3601 cctcactgat caagcactgg taaccactgc agtggtttag catttgcggc cgctgtcaga
     3661 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     3721 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     3781 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3841 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3901 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3961 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     4021 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     4081 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4141 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     4201 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     4261 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4321 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4381 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     4441 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     4501 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     4561 gcgcagcgag tcagtgagcg aggaagcgga agagcgctgc cggcacctgt cctacgagtt
     4621 gcatgataaa gaagacagtc ataagtgcgg cgacg
//
