LOCUS       sequence-169924-        4655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Diehl WE, Lin AE, Grubaugh ND, Carvalho LM, Kim K, Kyawe PP, 
            McCauley SM, Donnard E, Kucukural A, McDonel P, Schaffner SF, Garber
            M, Rambaut A, Andersen KG, Sabeti PC, Luban J
  TITLE     Ebola Virus Glycoprotein with Increased Infectivity Dominated the 
            2013-2016 Epidemic.
  JOURNAL   Cell. 2016 Nov 3;167(4):1088-1098.e6. doi: 
            10.1016/j.cell.2016.10.014.
  PUBMED    27814506
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.10"; journalName: "Cell"; date: "2016-11-3- 3";
            volume: "167"; issue: "4"; pages: "1088-1098.e6"
COMMENT     Sequence Label: pGL4.23 A82V/T230A/D637G 2014 EBOV
            Delta-Mucin...
FEATURES             Location/Qualifiers
     source          1..4655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      388..976
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     877..896
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1130..1147
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    1395..1516
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1432..1451)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1486..1505
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     regulatory      3040..3049
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3076..3107)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     primer_bind     complement(3141..3161)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3316..3695
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    3725..3816
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(3757..3776)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    3830..3878
                     /note="synthetic polyadenylation signal"
     CDS             join(3983..4051,4052..4655,1..188)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ctggctggtt cattgctgac aaatcggggg ccggtgaacg cggctctcgc ggcatcattg
       61 ctgcgctggg gcctgatggt aagccctcac gaatcgtagt gatctacacg acggggagtc
      121 aggccactat ggacgaacga aataggcaga tcgctgagat cggtgcctca ctgatcaagc
      181 actggtaacc actgcagtgg tttagcattt gcggccgctg tcagaccaag tttactcata
      241 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
      301 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
      361 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
      421 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
      481 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
      541 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
      601 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
      661 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
      721 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
      781 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
      841 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
      901 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
      961 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1021 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     1081 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     1141 gagcgaggaa gcggaagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga
     1201 cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt
     1261 tgaaggctct caagggcatc ggtcgacgga tccttatcga ttttaccaca tttgtagagg
     1321 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     1381 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     1441 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     1501 tcatcaatgt atcttatcat gtctgctcga agcggccggc cgccccgact ctagactaaa
     1561 agacaaattt gcatatacag aataaagcga taactgcaat tataacacct gtaactccaa
     1621 tacctgccgg tatccattgt ctccatcctg tccaccaatt gtcattgtcc ccctggcccg
     1681 gaagggtttt atcaacaaaa tcatgaataa tctgatcaat tttgtctgtt atgttcttgg
     1741 tccaatcatg tggttcgata cagcagtccg gtcccaaaat gtggcatgtg ccaccccatc
     1801 gctgcagcag gaagtcaatt gccttacggt tgaggattga aaaggttcgc agctcagttg
     1861 tggctctcag gaacagttgg agagcttgag tcgtttcgtt ggccagctgc ctcaacccac
     1921 agattaaacc atcttggttg tgcattagcc cctctgtgta aattccttcg gctgctggcc
     1981 cgaaatatgg tatccaggcc aatccgattg cagcaccttc atcctgagta gtccagtaat
     2041 gtaaattggg gttgcatttg ggttgagcat tgacaattac ttctcttcga gtccttctcc
     2101 cgcctgtgat cagtcctgcg aaagacaact cttcactgcg aatttttcta gtgaggtttt
     2161 ttttagtttc ccagaaggcc cactccccga ttgttgtatc aatttcgggg ttgaccttcc
     2221 aaattagttt tcccgtggtg ttgctcctct tcccacttgc atatattgtc tcattcagct
     2281 ggagcagaaa ctgtggtgtg aatcttgatt caagttggac gtaggtcaaa ttgtcaacct
     2341 cgaacaagta ctctgcctca ttagttccaa aaccggtagc ctgatatcta attgtggtag
     2401 aataatagcc actcgacggg tcctccgttg cattgaccgg ctctctcaag gggtgtgagc
     2461 tgaagaagtc cttcttagct tggggcagta tcagaaatgc aacgacacct tcagcgaaag
     2521 tcgttcctcg gtagataact gtggaagcaa gtcgatcata caggaagaaa gcaccctctt
     2581 tgtggaaggc aaagtctccg gcacatggtc ccgttcctga tactttgtgc acataccggc
     2641 accgggggaa gccccgaatc ccgtctggcg ctgctggtag acactcactc ccgtcaggtt
     2701 ttttgatttc aagattgtag cagttttcag cccattcacc agcttcataa ttgaccacct
     2761 ttggtgggac accggacctg aagccccatc ttttagtcac agatggcacg tcagttgcca
     2821 ctccattccc ctcgagattc agtccaactg atctcaattg atttgtggat gacagtttgt
     2881 cacgacaaac tagtttgtcg acatcactaa cctgtaatgt actattgtgg ataactccaa
     2941 gcgggatgga aaatgttctt tggaaaagga taattaccca aagaaagaat gatgtcctct
     3001 tgaatcgatc acgaggtaac tgcaatattc ctgtaacacc catggtggct ttaccaacag
     3061 taccggattg ccaagctgga agtcgagctt ccattatata ccctctagtg tctaagcttg
     3121 gccgccgagg ccccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt
     3181 acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat
     3241 ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg
     3301 ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg
     3361 aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac
     3421 gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta
     3481 ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac
     3541 atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt
     3601 taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat
     3661 tgattactat taataactag tcaataatca atgtcaacgc gtatatctgg cggccagtta
     3721 ggccagagaa atgttctggc acctgcactt gcactgggga cagcctattt tgctagtttg
     3781 ttttgtttcg ttttgttttg atggagagcg tatgttagta ctatcgattc acacaaaaaa
     3841 ccaacacaca gatgtaatga aaataaagat attttattgc ggcctgtcca atacctcccg
     3901 taccttaata ttacttactt atccttgaga gacgtactag taaccctgat aaatgcttca
     3961 ataatattga aaaaggacga gtatgagcat ccaacatttt cgtgtcgcac tcattccctt
     4021 ctttgcggca ttttgcttgc ctgtttttgc acaccccgaa acgctggtga aagtaaaaga
     4081 tgctgaagat caactgggtg caagagtggg ctatatcgaa ctggatctca atagcggcaa
     4141 gatccttgag tctttccgcc ccgaagaacg attcccgatg atgagcactt tcaaagtact
     4201 gctatgtggc gcggtgttgt cccgtataga cgccgggcaa gagcagcttg gtcgccgtat
     4261 acactactca caaaacgact tggttgagta ctcgccggtc acggaaaagc atcttacgga
     4321 tggcatgacg gtaagagaat tgtgtagtgc tgccattacc atgagcgaca acaccgcggc
     4381 caacttactt ctgacaacga tcggaggccc taaggagctg actgcatttc ttcataatat
     4441 gggtgatcat gtgacccggc ttgaccgctg ggaaccagag ttgaacgaag ccataccgaa
     4501 cgacgagcgt gataccacga tgccagtagc aatggccaca actcttcgga aactactcac
     4561 tggcgaactt cttactctag catcacgaca gcagctcata gactggatgg aggcggacaa
     4621 agtagcagga ccacttcttc gctcggccct ccctg
//
