LOCUS       sequence-169925-        5198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Diehl WE, Lin AE, Grubaugh ND, Carvalho LM, Kim K, Kyawe PP, 
            McCauley SM, Donnard E, Kucukural A, McDonel P, Schaffner SF, Garber
            M, Rambaut A, Andersen KG, Sabeti PC, Luban J
  TITLE     Ebola Virus Glycoprotein with Increased Infectivity Dominated the 
            2013-2016 Epidemic.
  JOURNAL   Cell. 2016 Nov 3;167(4):1088-1098.e6. doi: 
            10.1016/j.cell.2016.10.014.
  PUBMED    27814506
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.10"; journalName: "Cell"; date: "2016-11-3- 3";
            volume: "167"; issue: "4"; pages: "1088-1098.e6"
COMMENT     Sequence Label: pGL4.23 WT 2014 EBOV + Mucin-Like Domain
FEATURES             Location/Qualifiers
     source          1..5198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     405..424
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     658..675
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    923..1044
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(960..979)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1014..1033
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     regulatory      3111..3120
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3147..3178)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     primer_bind     complement(3212..3232)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3387..3766
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    3796..3887
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(3828..3847)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    3901..3949
                     /note="synthetic polyadenylation signal"
     CDS             join(4054..4122,4123..4914)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      join(5114..5198,1..504)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
       61 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
      121 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
      181 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
      241 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
      301 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
      361 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
      421 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
      481 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
      541 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
      601 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
      661 gagtcagtga gcgaggaagc ggaagagcgc tgccggcacc tgtcctacga gttgcatgat
      721 aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct
      781 gactgggttg aaggctctca agggcatcgg tcgacggatc cttatcgatt ttaccacatt
      841 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      901 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      961 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     1021 gtccaaactc atcaatgtat cttatcatgt ctgctcgaag cggccggccg ccccgactct
     1081 agactaaaag acaaatttgc atatacagaa taaagcgata actgcaatta taacacctgt
     1141 aactccaata cctgccggta tccattgtct ccatcctgtc caccaattgt cattgtcccc
     1201 ctggtccgga agggttttat caacaaaatc atgaataatc tgatcaattt tgtctgttat
     1261 gttcttggtc caatcatgtg gttcgataca gcagtccggt cccaaaatgt ggcatgtgcc
     1321 accccatcgc tgcagcagga agtcaattgc cttacggttg aggattgaaa aggttcgcag
     1381 ctcagttgtg gctctcagga acagttggag agcttgagtc gtttcgttgg ccagctgcct
     1441 caacccacag attaaaccat cttggttgtg cattagcccc tctgtgtaaa ttccttcggc
     1501 tgctggcccg aaatatggta tccaggccaa tccgattgca gcaccttcat cctgagtagt
     1561 ccagtaatgt aaattggggt tgcatttggg ttgagcattg acaattactt ctcttcgagt
     1621 ccttctcccg cctgtgatca gtcctgctac tccagcaata gtattggtaa ttaagcctag
     1681 cttcccgctg ctggcactct cttctccggt atcttggtga tgagtgttgt tgttgccagc
     1741 agtctcgctg tagttttggg ggcttgtcgt ggtggcgagg tccagggagt cagcgctctt
     1801 actcgtgttg gtgttctctg cttttaaggg tccggctgcg gtcgtggcgg ggggagtgtc
     1861 ggaggctgtg ctgtcgttgt ctgctctacg gtgatgttgt ccaacttgag ttgcctcaga
     1921 gatgtcaagt ttatacacgg gtgtattatg ggtgctgttg tccggacctg ttttggttgt
     1981 gggaggttga ggactcgtgg agattgtggc aagggttgtc agatgcgaca ctgcagcttt
     2041 ccttccttga ctgtgcactt gaaccattgc agaggaattt tctgaagcca tgattttgtg
     2101 gtcttcattt gttgtgttgg tctctgggtc ggaagaagtt cgcgccggac tctgaccact
     2161 gatgtttttg ggtccgtttg atacagctgt gaaagacaac tcttcactgc gaatttttct
     2221 agtgaggttt tttttagttt cccagaaggc ccactccccg attgttgtat caatttcggg
     2281 gttgaccttc caaattagtt ttcccgtggt gttgctcctc ttcccacttg catatattgt
     2341 ctcattcagc tggagcagaa actgtggtgt gaatcttgat tcaagttgga cgtaggtcaa
     2401 attgtcaacc tcgaacaagt actctgtctc attagttcca aaaccggtag cctgatatct
     2461 aattgtggta gaataatagc cactcgacgg gtcctccgtt gcattgaccg gctctctcaa
     2521 ggggtgtgag ctgaagaagt ccttcttagc ttggggcagt atcagaaatg caacgacacc
     2581 ttcagcgaaa gtcgttcctc ggtagataac tgtggaagca agtcgatcat acaggaagaa
     2641 agcaccctct ttgtggaagg caaagtctcc ggcacatggt cccgttcctg atactttgtg
     2701 cacataccgg caccggggga agccccgaat cccgtctggc gctgctggta gacactcact
     2761 cccgtcaggt tttttgattt caagattgta gcagttttca gcccattcac cagcttcata
     2821 attgaccacc tttggtggga caccggacct gaagccccat cttttagtcg cagatggcac
     2881 gtcagttgcc actccattcc cctcgagatt cagtccaact gatctcaatt gatttgtgga
     2941 tgacagtttg tcacgacaaa ctagtttgtc gacatcacta acctgtaatg tactattgtg
     3001 gataactcca agcgggatgg aaaatgttct ttggaaaagg ataattaccc aaagaaagaa
     3061 tgatgtcctc ttgaatcgat cacgaggtaa ctgcaatatt cctgtaacac ccatggtggc
     3121 tttaccaaca gtaccggatt gccaagctgg aagtcgagct tccattatat accctctagt
     3181 gtctaagctt ggccgccgag gccccaccgt acacgcctac cgcccatttg cgtcaatggg
     3241 gcggagttgt tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca
     3301 ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca
     3361 ttgatgtact gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac
     3421 tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta
     3481 ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag
     3541 tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt
     3601 actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca
     3661 ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat
     3721 gaccccgtaa ttgattacta ttaataacta gtcaataatc aatgtcaacg cgtatatctg
     3781 gcggccagtt aggccagaga aatgttctgg cacctgcact tgcactgggg acagcctatt
     3841 ttgctagttt gttttgtttc gttttgtttt gatggagagc gtatgttagt actatcgatt
     3901 cacacaaaaa accaacacac agatgtaatg aaaataaaga tattttattg cggcctgtcc
     3961 aatacctccc gtaccttaat attacttact tatccttgag agacgtacta gtaaccctga
     4021 taaatgcttc aataatattg aaaaaggacg agtatgagca tccaacattt tcgtgtcgca
     4081 ctcattccct tctttgcggc attttgcttg cctgtttttg cacaccccga aacgctggtg
     4141 aaagtaaaag atgctgaaga tcaactgggt gcaagagtgg gctatatcga actggatctc
     4201 aatagcggca agatccttga gtctttccgc cccgaagaac gattcccgat gatgagcact
     4261 ttcaaagtac tgctatgtgg cgcggtgttg tcccgtatag acgccgggca agagcagctt
     4321 ggtcgccgta tacactactc acaaaacgac ttggttgagt actcgccggt cacggaaaag
     4381 catcttacgg atggcatgac ggtaagagaa ttgtgtagtg ctgccattac catgagcgac
     4441 aacaccgcgg ccaacttact tctgacaacg atcggaggcc ctaaggagct gactgcattt
     4501 cttcataata tgggtgatca tgtgacccgg cttgaccgct gggaaccaga gttgaacgaa
     4561 gccataccga acgacgagcg tgataccacg atgccagtag caatggccac aactcttcgg
     4621 aaactactca ctggcgaact tcttactcta gcatcacgac agcagctcat agactggatg
     4681 gaggcggaca aagtagcagg accacttctt cgctcggccc tccctgctgg ctggttcatt
     4741 gctgacaaat cgggggccgg tgaacgcggc tctcgcggca tcattgctgc gctggggcct
     4801 gatggtaagc cctcacgaat cgtagtgatc tacacgacgg ggagtcaggc cactatggac
     4861 gaacgaaata ggcagatcgc tgagatcggt gcctcactga tcaagcactg gtaaccactg
     4921 cagtggttta gcatttgcgg ccgctgtcag accaagttta ctcatatata ctttagattg
     4981 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5041 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5101 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5161 aaccaccgct accagcggtg gtttgtttgc cggatcaa
//
