LOCUS       sequence-169926-        5198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Diehl WE, Lin AE, Grubaugh ND, Carvalho LM, Kim K, Kyawe PP, 
            McCauley SM, Donnard E, Kucukural A, McDonel P, Schaffner SF, Garber
            M, Rambaut A, Andersen KG, Sabeti PC, Luban J
  TITLE     Ebola Virus Glycoprotein with Increased Infectivity Dominated the 
            2013-2016 Epidemic.
  JOURNAL   Cell. 2016 Nov 3;167(4):1088-1098.e6. doi: 
            10.1016/j.cell.2016.10.014.
  PUBMED    27814506
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.10"; journalName: "Cell"; date: "2016-11-3- 3";
            volume: "167"; issue: "4"; pages: "1088-1098.e6"
COMMENT     Sequence Label: pGL4.23 A82V/T230A/D637G 2014 EBOV + Mucin-Lik...
FEATURES             Location/Qualifiers
     source          1..5198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(48..636)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(128..147)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(836..1627,1628..1696))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    1801..1849
                     /note="synthetic polyadenylation signal"
     misc_feature    1863..1954
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     1903..1922
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     enhancer        1984..2363
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2518..2538
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        2572..2603
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      2630..2639
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4706..4827
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4717..4736)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4771..4790
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5075..5092)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
       61 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      121 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      181 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      241 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
      301 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
      361 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
      421 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
      481 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
      541 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
      601 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
      661 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
      721 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
      781 atatatgagt aaacttggtc tgacagcggc cgcaaatgct aaaccactgc agtggttacc
      841 agtgcttgat cagtgaggca ccgatctcag cgatctgcct atttcgttcg tccatagtgg
      901 cctgactccc cgtcgtgtag atcactacga ttcgtgaggg cttaccatca ggccccagcg
      961 cagcaatgat gccgcgagag ccgcgttcac cggcccccga tttgtcagca atgaaccagc
     1021 cagcagggag ggccgagcga agaagtggtc ctgctacttt gtccgcctcc atccagtcta
     1081 tgagctgctg tcgtgatgct agagtaagaa gttcgccagt gagtagtttc cgaagagttg
     1141 tggccattgc tactggcatc gtggtatcac gctcgtcgtt cggtatggct tcgttcaact
     1201 ctggttccca gcggtcaagc cgggtcacat gatcacccat attatgaaga aatgcagtca
     1261 gctccttagg gcctccgatc gttgtcagaa gtaagttggc cgcggtgttg tcgctcatgg
     1321 taatggcagc actacacaat tctcttaccg tcatgccatc cgtaagatgc ttttccgtga
     1381 ccggcgagta ctcaaccaag tcgttttgtg agtagtgtat acggcgacca agctgctctt
     1441 gcccggcgtc tatacgggac aacaccgcgc cacatagcag tactttgaaa gtgctcatca
     1501 tcgggaatcg ttcttcgggg cggaaagact caaggatctt gccgctattg agatccagtt
     1561 cgatatagcc cactcttgca cccagttgat cttcagcatc ttttactttc accagcgttt
     1621 cggggtgtgc aaaaacaggc aagcaaaatg ccgcaaagaa gggaatgagt gcgacacgaa
     1681 aatgttggat gctcatactc gtcctttttc aatattattg aagcatttat cagggttact
     1741 agtacgtctc tcaaggataa gtaagtaata ttaaggtacg ggaggtattg gacaggccgc
     1801 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta
     1861 ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc
     1921 cccagtgcaa gtgcaggtgc cagaacattt ctctggccta actggccgcc agatatacgc
     1981 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     2041 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     2101 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     2161 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     2221 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     2281 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     2341 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     2401 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     2461 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     2521 aaatgggcgg taggcgtgta cggtggggcc tcggcggcca agcttagaca ctagagggta
     2581 tataatggaa gctcgacttc cagcttggca atccggtact gttggtaaag ccaccatggg
     2641 tgttacagga atattgcagt tacctcgtga tcgattcaag aggacatcat tctttctttg
     2701 ggtaattatc cttttccaaa gaacattttc catcccgctt ggagttatcc acaatagtac
     2761 attacaggtt agtgatgtcg acaaactagt ttgtcgtgac aaactgtcat ccacaaatca
     2821 attgagatca gttggactga atctcgaggg gaatggagtg gcaactgacg tgccatctgt
     2881 gactaaaaga tggggcttca ggtccggtgt cccaccaaag gtggtcaatt atgaagctgg
     2941 tgaatgggct gaaaactgct acaatcttga aatcaaaaaa cctgacggga gtgagtgtct
     3001 accagcagcg ccagacggga ttcggggctt cccccggtgc cggtatgtgc acaaagtatc
     3061 aggaacggga ccatgtgccg gagactttgc cttccacaaa gagggtgctt tcttcctgta
     3121 tgatcgactt gcttccacag ttatctaccg aggaacgact ttcgctgaag gtgtcgttgc
     3181 atttctgata ctgccccaag ctaagaagga cttcttcagc tcacacccct tgagagagcc
     3241 ggtcaatgca acggaggacc cgtcgagtgg ctattattct accacaatta gatatcaggc
     3301 taccggtttt ggaactaatg aggcagagta cttgttcgag gttgacaatt tgacctacgt
     3361 ccaacttgaa tcaagattca caccacagtt tctgctccag ctgaatgaga caatatatgc
     3421 aagtgggaag aggagcaaca ccacgggaaa actaatttgg aaggtcaacc ccgaaattga
     3481 tacaacaatc ggggagtggg ccttctggga aactaaaaaa aacctcacta gaaaaattcg
     3541 cagtgaagag ttgtctttca cagctgtatc aaacggaccc aaaaacatca gtggtcagag
     3601 tccggcgcga acttcttccg acccagagac caacacaaca aatgaagacc acaaaatcat
     3661 ggcttcagaa aattcctctg caatggttca agtgcacagt caaggaagga aagctgcagt
     3721 gtcgcatctg acaacccttg ccacaatctc cacgagtcct caacctccca caaccaaaac
     3781 aggtccggac aacagcaccc ataatacacc cgtgtataaa cttgacatct ctgaggcaac
     3841 tcaagttgga caacatcacc gtagagcaga caacgacagc acagcctccg acactccccc
     3901 cgccacgacc gcagccggac ccttaaaagc agagaacacc aacacgagta agagcgctga
     3961 ctccctggac ctcgccacca cgacaagccc ccaaaactac agcgagactg ctggcaacaa
     4021 caacactcat caccaagata ccggagaaga gagtgccagc agcgggaagc taggcttaat
     4081 taccaatact attgctggag tagcaggact gatcacaggc gggagaagga ctcgaagaga
     4141 agtaattgtc aatgctcaac ccaaatgcaa ccccaattta cattactgga ctactcagga
     4201 tgaaggtgct gcaatcggat tggcctggat accatatttc gggccagcag ccgaaggaat
     4261 ttacacagag gggctaatgc acaaccaaga tggtttaatc tgtgggttga ggcagctggc
     4321 caacgaaacg actcaagctc tccaactgtt cctgagagcc acaactgagc tgcgaacctt
     4381 ttcaatcctc aaccgtaagg caattgactt cctgctgcag cgatggggtg gcacatgcca
     4441 cattttggga ccggactgct gtatcgaacc acatgattgg accaagaaca taacagacaa
     4501 aattgatcag attattcatg attttgttga taaaaccctt ccgggccagg gggacaatga
     4561 caattggtgg acaggatgga gacaatggat accggcaggt attggagtta caggtgttat
     4621 aattgcagtt atcgctttat tctgtatatg caaatttgtc ttttagtcta gagtcggggc
     4681 ggccggccgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact
     4741 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     4801 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     4861 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa
     4921 atcgataagg atccgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg
     4981 gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact
     5041 cgtaggacag gtgccggcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     5101 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     5161 aatcagggga taacgcagga aagaacatgt gagcaaaa
//
