LOCUS       sequence-169927-        5198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Diehl WE, Lin AE, Grubaugh ND, Carvalho LM, Kim K, Kyawe PP, 
            McCauley SM, Donnard E, Kucukural A, McDonel P, Schaffner SF, Garber
            M, Rambaut A, Andersen KG, Sabeti PC, Luban J
  TITLE     Ebola Virus Glycoprotein with Increased Infectivity Dominated the 
            2013-2016 Epidemic.
  JOURNAL   Cell. 2016 Nov 3;167(4):1088-1098.e6. doi: 
            10.1016/j.cell.2016.10.014.
  PUBMED    27814506
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.10"; journalName: "Cell"; date: "2016-11-3- 3";
            volume: "167"; issue: "4"; pages: "1088-1098.e6"
COMMENT     Sequence Label: pGL4.23 T230A 2014 EBOV + Mucin-Like Domain
FEATURES             Location/Qualifiers
     source          1..5198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        43..422
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    452..543
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(484..503)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    557..605
                     /note="synthetic polyadenylation signal"
     CDS             join(710..778,779..1570)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1770..2358
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2259..2278
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2512..2529
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    2777..2898
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2814..2833)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2868..2887
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     regulatory      4965..4974
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5001..5032)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     primer_bind     complement(5066..5086)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcgatgac
       61 taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg
      121 ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac
      181 ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa
      241 agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc
      301 gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata
      361 tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg
      421 tcaacgcgta tatctggcgg ccagttaggc cagagaaatg ttctggcacc tgcacttgca
      481 ctggggacag cctattttgc tagtttgttt tgtttcgttt tgttttgatg gagagcgtat
      541 gttagtacta tcgattcaca caaaaaacca acacacagat gtaatgaaaa taaagatatt
      601 ttattgcggc ctgtccaata cctcccgtac cttaatatta cttacttatc cttgagagac
      661 gtactagtaa ccctgataaa tgcttcaata atattgaaaa aggacgagta tgagcatcca
      721 acattttcgt gtcgcactca ttcccttctt tgcggcattt tgcttgcctg tttttgcaca
      781 ccccgaaacg ctggtgaaag taaaagatgc tgaagatcaa ctgggtgcaa gagtgggcta
      841 tatcgaactg gatctcaata gcggcaagat ccttgagtct ttccgccccg aagaacgatt
      901 cccgatgatg agcactttca aagtactgct atgtggcgcg gtgttgtccc gtatagacgc
      961 cgggcaagag cagcttggtc gccgtataca ctactcacaa aacgacttgg ttgagtactc
     1021 gccggtcacg gaaaagcatc ttacggatgg catgacggta agagaattgt gtagtgctgc
     1081 cattaccatg agcgacaaca ccgcggccaa cttacttctg acaacgatcg gaggccctaa
     1141 ggagctgact gcatttcttc ataatatggg tgatcatgtg acccggcttg accgctggga
     1201 accagagttg aacgaagcca taccgaacga cgagcgtgat accacgatgc cagtagcaat
     1261 ggccacaact cttcggaaac tactcactgg cgaacttctt actctagcat cacgacagca
     1321 gctcatagac tggatggagg cggacaaagt agcaggacca cttcttcgct cggccctccc
     1381 tgctggctgg ttcattgctg acaaatcggg ggccggtgaa cgcggctctc gcggcatcat
     1441 tgctgcgctg gggcctgatg gtaagccctc acgaatcgta gtgatctaca cgacggggag
     1501 tcaggccact atggacgaac gaaataggca gatcgctgag atcggtgcct cactgatcaa
     1561 gcactggtaa ccactgcagt ggtttagcat ttgcggccgc tgtcagacca agtttactca
     1621 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     1681 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1741 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1801 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1861 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     1921 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     1981 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     2041 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     2101 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     2161 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     2221 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     2281 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     2341 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     2401 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     2461 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     2521 gtgagcgagg aagcggaaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa
     2581 gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg
     2641 gttgaaggct ctcaagggca tcggtcgacg gatccttatc gattttacca catttgtaga
     2701 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     2761 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     2821 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     2881 actcatcaat gtatcttatc atgtctgctc gaagcggccg gccgccccga ctctagacta
     2941 aaagacaaat ttgcatatac agaataaagc gataactgca attataacac ctgtaactcc
     3001 aatacctgcc ggtatccatt gtctccatcc tgtccaccaa ttgtcattgt ccccctggtc
     3061 cggaagggtt ttatcaacaa aatcatgaat aatctgatca attttgtctg ttatgttctt
     3121 ggtccaatca tgtggttcga tacagcagtc cggtcccaaa atgtggcatg tgccacccca
     3181 tcgctgcagc aggaagtcaa ttgccttacg gttgaggatt gaaaaggttc gcagctcagt
     3241 tgtggctctc aggaacagtt ggagagcttg agtcgtttcg ttggccagct gcctcaaccc
     3301 acagattaaa ccatcttggt tgtgcattag cccctctgtg taaattcctt cggctgctgg
     3361 cccgaaatat ggtatccagg ccaatccgat tgcagcacct tcatcctgag tagtccagta
     3421 atgtaaattg gggttgcatt tgggttgagc attgacaatt acttctcttc gagtccttct
     3481 cccgcctgtg atcagtcctg ctactccagc aatagtattg gtaattaagc ctagcttccc
     3541 gctgctggca ctctcttctc cggtatcttg gtgatgagtg ttgttgttgc cagcagtctc
     3601 gctgtagttt tgggggcttg tcgtggtggc gaggtccagg gagtcagcgc tcttactcgt
     3661 gttggtgttc tctgctttta agggtccggc tgcggtcgtg gcggggggag tgtcggaggc
     3721 tgtgctgtcg ttgtctgctc tacggtgatg ttgtccaact tgagttgcct cagagatgtc
     3781 aagtttatac acgggtgtat tatgggtgct gttgtccgga cctgttttgg ttgtgggagg
     3841 ttgaggactc gtggagattg tggcaagggt tgtcagatgc gacactgcag ctttccttcc
     3901 ttgactgtgc acttgaacca ttgcagagga attttctgaa gccatgattt tgtggtcttc
     3961 atttgttgtg ttggtctctg ggtcggaaga agttcgcgcc ggactctgac cactgatgtt
     4021 tttgggtccg tttgatacag ctgtgaaaga caactcttca ctgcgaattt ttctagtgag
     4081 gtttttttta gtttcccaga aggcccactc cccgattgtt gtatcaattt cggggttgac
     4141 cttccaaatt agttttcccg tggtgttgct cctcttccca cttgcatata ttgtctcatt
     4201 cagctggagc agaaactgtg gtgtgaatct tgattcaagt tggacgtagg tcaaattgtc
     4261 aacctcgaac aagtactctg cctcattagt tccaaaaccg gtagcctgat atctaattgt
     4321 ggtagaataa tagccactcg acgggtcctc cgttgcattg accggctctc tcaaggggtg
     4381 tgagctgaag aagtccttct tagcttgggg cagtatcaga aatgcaacga caccttcagc
     4441 gaaagtcgtt cctcggtaga taactgtgga agcaagtcga tcatacagga agaaagcacc
     4501 ctctttgtgg aaggcaaagt ctccggcaca tggtcccgtt cctgatactt tgtgcacata
     4561 ccggcaccgg gggaagcccc gaatcccgtc tggcgctgct ggtagacact cactcccgtc
     4621 aggttttttg atttcaagat tgtagcagtt ttcagcccat tcaccagctt cataattgac
     4681 cacctttggt gggacaccgg acctgaagcc ccatctttta gtcgcagatg gcacgtcagt
     4741 tgccactcca ttcccctcga gattcagtcc aactgatctc aattgatttg tggatgacag
     4801 tttgtcacga caaactagtt tgtcgacatc actaacctgt aatgtactat tgtggataac
     4861 tccaagcggg atggaaaatg ttctttggaa aaggataatt acccaaagaa agaatgatgt
     4921 cctcttgaat cgatcacgag gtaactgcaa tattcctgta acacccatgg tggctttacc
     4981 aacagtaccg gattgccaag ctggaagtcg agcttccatt atataccctc tagtgtctaa
     5041 gcttggccgc cgaggcccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag
     5101 ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg
     5161 tcaatggggt ggagacttgg aaatccccgt gagtcaaa
//
