LOCUS       sequence-169928-        5198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Diehl WE, Lin AE, Grubaugh ND, Carvalho LM, Kim K, Kyawe PP, 
            McCauley SM, Donnard E, Kucukural A, McDonel P, Schaffner SF, Garber
            M, Rambaut A, Andersen KG, Sabeti PC, Luban J
  TITLE     Ebola Virus Glycoprotein with Increased Infectivity Dominated the 
            2013-2016 Epidemic.
  JOURNAL   Cell. 2016 Nov 3;167(4):1088-1098.e6. doi: 
            10.1016/j.cell.2016.10.014.
  PUBMED    27814506
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.10"; journalName: "Cell"; date: "2016-11-3- 3";
            volume: "167"; issue: "4"; pages: "1088-1098.e6"
COMMENT     Sequence Label: pGL4.23 A82V 2014 EBOV + Mucin-Like Domain
FEATURES             Location/Qualifiers
     source          1..5198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(17..48)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     primer_bind     complement(82..102)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        257..636
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    666..757
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(698..717)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    771..819
                     /note="synthetic polyadenylation signal"
     CDS             join(924..992,993..1784)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1984..2572
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2473..2492
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2726..2743
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    2991..3112
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3028..3047)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3082..3101
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     regulatory      5179..5188
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtaccggatt gccaagctgg aagtcgagct tccattatat accctctagt gtctaagctt
       61 ggccgccgag gccccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt
      121 tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa
      181 tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact
      241 gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag
      301 gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga
      361 cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt
      421 accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa
      481 catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat
      541 ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa
      601 ttgattacta ttaataacta gtcaataatc aatgtcaacg cgtatatctg gcggccagtt
      661 aggccagaga aatgttctgg cacctgcact tgcactgggg acagcctatt ttgctagttt
      721 gttttgtttc gttttgtttt gatggagagc gtatgttagt actatcgatt cacacaaaaa
      781 accaacacac agatgtaatg aaaataaaga tattttattg cggcctgtcc aatacctccc
      841 gtaccttaat attacttact tatccttgag agacgtacta gtaaccctga taaatgcttc
      901 aataatattg aaaaaggacg agtatgagca tccaacattt tcgtgtcgca ctcattccct
      961 tctttgcggc attttgcttg cctgtttttg cacaccccga aacgctggtg aaagtaaaag
     1021 atgctgaaga tcaactgggt gcaagagtgg gctatatcga actggatctc aatagcggca
     1081 agatccttga gtctttccgc cccgaagaac gattcccgat gatgagcact ttcaaagtac
     1141 tgctatgtgg cgcggtgttg tcccgtatag acgccgggca agagcagctt ggtcgccgta
     1201 tacactactc acaaaacgac ttggttgagt actcgccggt cacggaaaag catcttacgg
     1261 atggcatgac ggtaagagaa ttgtgtagtg ctgccattac catgagcgac aacaccgcgg
     1321 ccaacttact tctgacaacg atcggaggcc ctaaggagct gactgcattt cttcataata
     1381 tgggtgatca tgtgacccgg cttgaccgct gggaaccaga gttgaacgaa gccataccga
     1441 acgacgagcg tgataccacg atgccagtag caatggccac aactcttcgg aaactactca
     1501 ctggcgaact tcttactcta gcatcacgac agcagctcat agactggatg gaggcggaca
     1561 aagtagcagg accacttctt cgctcggccc tccctgctgg ctggttcatt gctgacaaat
     1621 cgggggccgg tgaacgcggc tctcgcggca tcattgctgc gctggggcct gatggtaagc
     1681 cctcacgaat cgtagtgatc tacacgacgg ggagtcaggc cactatggac gaacgaaata
     1741 ggcagatcgc tgagatcggt gcctcactga tcaagcactg gtaaccactg cagtggttta
     1801 gcatttgcgg ccgctgtcag accaagttta ctcatatata ctttagattg atttaaaact
     1861 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     1921 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1981 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     2041 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     2101 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     2161 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     2221 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     2281 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     2341 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     2401 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     2461 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     2521 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     2581 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     2641 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     2701 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgctg
     2761 ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag
     2821 tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc
     2881 gacggatcct tatcgatttt accacatttg tagaggtttt acttgcttta aaaaacctcc
     2941 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     3001 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     3061 ttttttcatt gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     3121 gctcgaagcg gccggccgcc ccgactctag actaaaagac aaatttgcat atacagaata
     3181 aagcgataac tgcaattata acacctgtaa ctccaatacc tgccggtatc cattgtctcc
     3241 atcctgtcca ccaattgtca ttgtccccct ggtccggaag ggttttatca acaaaatcat
     3301 gaataatctg atcaattttg tctgttatgt tcttggtcca atcatgtggt tcgatacagc
     3361 agtccggtcc caaaatgtgg catgtgccac cccatcgctg cagcaggaag tcaattgcct
     3421 tacggttgag gattgaaaag gttcgcagct cagttgtggc tctcaggaac agttggagag
     3481 cttgagtcgt ttcgttggcc agctgcctca acccacagat taaaccatct tggttgtgca
     3541 ttagcccctc tgtgtaaatt ccttcggctg ctggcccgaa atatggtatc caggccaatc
     3601 cgattgcagc accttcatcc tgagtagtcc agtaatgtaa attggggttg catttgggtt
     3661 gagcattgac aattacttct cttcgagtcc ttctcccgcc tgtgatcagt cctgctactc
     3721 cagcaatagt attggtaatt aagcctagct tcccgctgct ggcactctct tctccggtat
     3781 cttggtgatg agtgttgttg ttgccagcag tctcgctgta gttttggggg cttgtcgtgg
     3841 tggcgaggtc cagggagtca gcgctcttac tcgtgttggt gttctctgct tttaagggtc
     3901 cggctgcggt cgtggcgggg ggagtgtcgg aggctgtgct gtcgttgtct gctctacggt
     3961 gatgttgtcc aacttgagtt gcctcagaga tgtcaagttt atacacgggt gtattatggg
     4021 tgctgttgtc cggacctgtt ttggttgtgg gaggttgagg actcgtggag attgtggcaa
     4081 gggttgtcag atgcgacact gcagctttcc ttccttgact gtgcacttga accattgcag
     4141 aggaattttc tgaagccatg attttgtggt cttcatttgt tgtgttggtc tctgggtcgg
     4201 aagaagttcg cgccggactc tgaccactga tgtttttggg tccgtttgat acagctgtga
     4261 aagacaactc ttcactgcga atttttctag tgaggttttt tttagtttcc cagaaggccc
     4321 actccccgat tgttgtatca atttcggggt tgaccttcca aattagtttt cccgtggtgt
     4381 tgctcctctt cccacttgca tatattgtct cattcagctg gagcagaaac tgtggtgtga
     4441 atcttgattc aagttggacg taggtcaaat tgtcaacctc gaacaagtac tctgtctcat
     4501 tagttccaaa accggtagcc tgatatctaa ttgtggtaga ataatagcca ctcgacgggt
     4561 cctccgttgc attgaccggc tctctcaagg ggtgtgagct gaagaagtcc ttcttagctt
     4621 ggggcagtat cagaaatgca acgacacctt cagcgaaagt cgttcctcgg tagataactg
     4681 tggaagcaag tcgatcatac aggaagaaag caccctcttt gtggaaggca aagtctccgg
     4741 cacatggtcc cgttcctgat actttgtgca cataccggca ccgggggaag ccccgaatcc
     4801 cgtctggcgc tgctggtaga cactcactcc cgtcaggttt tttgatttca agattgtagc
     4861 agttttcagc ccattcacca gcttcataat tgaccacctt tggtgggaca ccggacctga
     4921 agccccatct tttagtcaca gatggcacgt cagttgccac tccattcccc tcgagattca
     4981 gtccaactga tctcaattga tttgtggatg acagtttgtc acgacaaact agtttgtcga
     5041 catcactaac ctgtaatgta ctattgtgga taactccaag cgggatggaa aatgttcttt
     5101 ggaaaaggat aattacccaa agaaagaatg atgtcctctt gaatcgatca cgaggtaact
     5161 gcaatattcc tgtaacaccc atggtggctt taccaaca
//
