LOCUS       sequence-169929-        5198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Diehl WE, Lin AE, Grubaugh ND, Carvalho LM, Kim K, Kyawe PP, 
            McCauley SM, Donnard E, Kucukural A, McDonel P, Schaffner SF, Garber
            M, Rambaut A, Andersen KG, Sabeti PC, Luban J
  TITLE     Ebola Virus Glycoprotein with Increased Infectivity Dominated the 
            2013-2016 Epidemic.
  JOURNAL   Cell. 2016 Nov 3;167(4):1088-1098.e6. doi: 
            10.1016/j.cell.2016.10.014.
  PUBMED    27814506
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.10"; journalName: "Cell"; date: "2016-11-3- 3";
            volume: "167"; issue: "4"; pages: "1088-1098.e6"
COMMENT     Sequence Label: pGL4.23 A82V/T230A 2014 EBOV + Mucin-Like Domain
FEATURES             Location/Qualifiers
     source          1..5198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     388..408
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        442..473
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      500..509
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2576..2697
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2587..2606)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2641..2660
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2945..2962)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3116..3704)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3196..3215)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3904..4695,4696..4764))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4869..4917
                     /note="synthetic polyadenylation signal"
     misc_feature    4931..5022
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4971..4990
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     enhancer        join(5052..5198,1..233)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
       61 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      121 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      181 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      241 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      301 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      361 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtggggcc
      421 tcggcggcca agcttagaca ctagagggta tataatggaa gctcgacttc cagcttggca
      481 atccggtact gttggtaaag ccaccatggg tgttacagga atattgcagt tacctcgtga
      541 tcgattcaag aggacatcat tctttctttg ggtaattatc cttttccaaa gaacattttc
      601 catcccgctt ggagttatcc acaatagtac attacaggtt agtgatgtcg acaaactagt
      661 ttgtcgtgac aaactgtcat ccacaaatca attgagatca gttggactga atctcgaggg
      721 gaatggagtg gcaactgacg tgccatctgt gactaaaaga tggggcttca ggtccggtgt
      781 cccaccaaag gtggtcaatt atgaagctgg tgaatgggct gaaaactgct acaatcttga
      841 aatcaaaaaa cctgacggga gtgagtgtct accagcagcg ccagacggga ttcggggctt
      901 cccccggtgc cggtatgtgc acaaagtatc aggaacggga ccatgtgccg gagactttgc
      961 cttccacaaa gagggtgctt tcttcctgta tgatcgactt gcttccacag ttatctaccg
     1021 aggaacgact ttcgctgaag gtgtcgttgc atttctgata ctgccccaag ctaagaagga
     1081 cttcttcagc tcacacccct tgagagagcc ggtcaatgca acggaggacc cgtcgagtgg
     1141 ctattattct accacaatta gatatcaggc taccggtttt ggaactaatg aggcagagta
     1201 cttgttcgag gttgacaatt tgacctacgt ccaacttgaa tcaagattca caccacagtt
     1261 tctgctccag ctgaatgaga caatatatgc aagtgggaag aggagcaaca ccacgggaaa
     1321 actaatttgg aaggtcaacc ccgaaattga tacaacaatc ggggagtggg ccttctggga
     1381 aactaaaaaa aacctcacta gaaaaattcg cagtgaagag ttgtctttca cagctgtatc
     1441 aaacggaccc aaaaacatca gtggtcagag tccggcgcga acttcttccg acccagagac
     1501 caacacaaca aatgaagacc acaaaatcat ggcttcagaa aattcctctg caatggttca
     1561 agtgcacagt caaggaagga aagctgcagt gtcgcatctg acaacccttg ccacaatctc
     1621 cacgagtcct caacctccca caaccaaaac aggtccggac aacagcaccc ataatacacc
     1681 cgtgtataaa cttgacatct ctgaggcaac tcaagttgga caacatcacc gtagagcaga
     1741 caacgacagc acagcctccg acactccccc cgccacgacc gcagccggac ccttaaaagc
     1801 agagaacacc aacacgagta agagcgctga ctccctggac ctcgccacca cgacaagccc
     1861 ccaaaactac agcgagactg ctggcaacaa caacactcat caccaagata ccggagaaga
     1921 gagtgccagc agcgggaagc taggcttaat taccaatact attgctggag tagcaggact
     1981 gatcacaggc gggagaagga ctcgaagaga agtaattgtc aatgctcaac ccaaatgcaa
     2041 ccccaattta cattactgga ctactcagga tgaaggtgct gcaatcggat tggcctggat
     2101 accatatttc gggccagcag ccgaaggaat ttacacagag gggctaatgc acaaccaaga
     2161 tggtttaatc tgtgggttga ggcagctggc caacgaaacg actcaagctc tccaactgtt
     2221 cctgagagcc acaactgagc tgcgaacctt ttcaatcctc aaccgtaagg caattgactt
     2281 cctgctgcag cgatggggtg gcacatgcca cattttggga ccggactgct gtatcgaacc
     2341 acatgattgg accaagaaca taacagacaa aattgatcag attattcatg attttgttga
     2401 taaaaccctt ccggaccagg gggacaatga caattggtgg acaggatgga gacaatggat
     2461 accggcaggt attggagtta caggtgttat aattgcagtt atcgctttat tctgtatatg
     2521 caaatttgtc ttttagtcta gagtcggggc ggccggccgc ttcgagcaga catgataaga
     2581 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     2641 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     2701 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     2761 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgtcgac cgatgccctt
     2821 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
     2881 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg
     2941 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtattagctc
     3001 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     3061 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     3121 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3181 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3241 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3301 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3361 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     3421 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     3481 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     3541 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     3601 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     3661 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     3721 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     3781 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     3841 tctaaagtat atatgagtaa acttggtctg acagcggccg caaatgctaa accactgcag
     3901 tggttaccag tgcttgatca gtgaggcacc gatctcagcg atctgcctat ttcgttcgtc
     3961 catagtggcc tgactccccg tcgtgtagat cactacgatt cgtgagggct taccatcagg
     4021 ccccagcgca gcaatgatgc cgcgagagcc gcgttcaccg gcccccgatt tgtcagcaat
     4081 gaaccagcca gcagggaggg ccgagcgaag aagtggtcct gctactttgt ccgcctccat
     4141 ccagtctatg agctgctgtc gtgatgctag agtaagaagt tcgccagtga gtagtttccg
     4201 aagagttgtg gccattgcta ctggcatcgt ggtatcacgc tcgtcgttcg gtatggcttc
     4261 gttcaactct ggttcccagc ggtcaagccg ggtcacatga tcacccatat tatgaagaaa
     4321 tgcagtcagc tccttagggc ctccgatcgt tgtcagaagt aagttggccg cggtgttgtc
     4381 gctcatggta atggcagcac tacacaattc tcttaccgtc atgccatccg taagatgctt
     4441 ttccgtgacc ggcgagtact caaccaagtc gttttgtgag tagtgtatac ggcgaccaag
     4501 ctgctcttgc ccggcgtcta tacgggacaa caccgcgcca catagcagta ctttgaaagt
     4561 gctcatcatc gggaatcgtt cttcggggcg gaaagactca aggatcttgc cgctattgag
     4621 atccagttcg atatagccca ctcttgcacc cagttgatct tcagcatctt ttactttcac
     4681 cagcgtttcg gggtgtgcaa aaacaggcaa gcaaaatgcc gcaaagaagg gaatgagtgc
     4741 gacacgaaaa tgttggatgc tcatactcgt cctttttcaa tattattgaa gcatttatca
     4801 gggttactag tacgtctctc aaggataagt aagtaatatt aaggtacggg aggtattgga
     4861 caggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat
     4921 cgatagtact aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat
     4981 aggctgtccc cagtgcaagt gcaggtgcca gaacatttct ctggcctaac tggccgccag
     5041 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     5101 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     5161 ctgaccgccc aacgaccccc gcccattgac gtcaataa
//
