LOCUS       sequence-169930-        5198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Diehl WE, Lin AE, Grubaugh ND, Carvalho LM, Kim K, Kyawe PP, 
            McCauley SM, Donnard E, Kucukural A, McDonel P, Schaffner SF, Garber
            M, Rambaut A, Andersen KG, Sabeti PC, Luban J
  TITLE     Ebola Virus Glycoprotein with Increased Infectivity Dominated the 
            2013-2016 Epidemic.
  JOURNAL   Cell. 2016 Nov 3;167(4):1088-1098.e6. doi: 
            10.1016/j.cell.2016.10.014.
  PUBMED    27814506
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.10"; journalName: "Cell"; date: "2016-11-3- 3";
            volume: "167"; issue: "4"; pages: "1088-1098.e6"
COMMENT     Sequence Label: pGL4.23 D637G 2014 EBOV + Mucin-Like Domain
FEATURES             Location/Qualifiers
     source          1..5198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     303..322
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     556..573
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    821..942
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(858..877)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     912..931
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     regulatory      3009..3018
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3045..3076)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     primer_bind     complement(3110..3130)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3285..3664
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    3694..3785
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(3726..3745)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    3799..3847
                     /note="synthetic polyadenylation signal"
     CDS             join(3952..4020,4021..4812)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      join(5012..5198,1..402)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
       61 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
      121 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
      181 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
      241 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      301 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      361 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
      421 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
      481 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
      541 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgctg ccggcacctg
      601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
      661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacggatcct
      721 tatcgatttt accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
      781 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
      841 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
      901 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gctcgaagcg
      961 gccggccgcc ccgactctag actaaaagac aaatttgcat atacagaata aagcgataac
     1021 tgcaattata acacctgtaa ctccaatacc tgccggtatc cattgtctcc atcctgtcca
     1081 ccaattgtca ttgtccccct ggcccggaag ggttttatca acaaaatcat gaataatctg
     1141 atcaattttg tctgttatgt tcttggtcca atcatgtggt tcgatacagc agtccggtcc
     1201 caaaatgtgg catgtgccac cccatcgctg cagcaggaag tcaattgcct tacggttgag
     1261 gattgaaaag gttcgcagct cagttgtggc tctcaggaac agttggagag cttgagtcgt
     1321 ttcgttggcc agctgcctca acccacagat taaaccatct tggttgtgca ttagcccctc
     1381 tgtgtaaatt ccttcggctg ctggcccgaa atatggtatc caggccaatc cgattgcagc
     1441 accttcatcc tgagtagtcc agtaatgtaa attggggttg catttgggtt gagcattgac
     1501 aattacttct cttcgagtcc ttctcccgcc tgtgatcagt cctgctactc cagcaatagt
     1561 attggtaatt aagcctagct tcccgctgct ggcactctct tctccggtat cttggtgatg
     1621 agtgttgttg ttgccagcag tctcgctgta gttttggggg cttgtcgtgg tggcgaggtc
     1681 cagggagtca gcgctcttac tcgtgttggt gttctctgct tttaagggtc cggctgcggt
     1741 cgtggcgggg ggagtgtcgg aggctgtgct gtcgttgtct gctctacggt gatgttgtcc
     1801 aacttgagtt gcctcagaga tgtcaagttt atacacgggt gtattatggg tgctgttgtc
     1861 cggacctgtt ttggttgtgg gaggttgagg actcgtggag attgtggcaa gggttgtcag
     1921 atgcgacact gcagctttcc ttccttgact gtgcacttga accattgcag aggaattttc
     1981 tgaagccatg attttgtggt cttcatttgt tgtgttggtc tctgggtcgg aagaagttcg
     2041 cgccggactc tgaccactga tgtttttggg tccgtttgat acagctgtga aagacaactc
     2101 ttcactgcga atttttctag tgaggttttt tttagtttcc cagaaggccc actccccgat
     2161 tgttgtatca atttcggggt tgaccttcca aattagtttt cccgtggtgt tgctcctctt
     2221 cccacttgca tatattgtct cattcagctg gagcagaaac tgtggtgtga atcttgattc
     2281 aagttggacg taggtcaaat tgtcaacctc gaacaagtac tctgtctcat tagttccaaa
     2341 accggtagcc tgatatctaa ttgtggtaga ataatagcca ctcgacgggt cctccgttgc
     2401 attgaccggc tctctcaagg ggtgtgagct gaagaagtcc ttcttagctt ggggcagtat
     2461 cagaaatgca acgacacctt cagcgaaagt cgttcctcgg tagataactg tggaagcaag
     2521 tcgatcatac aggaagaaag caccctcttt gtggaaggca aagtctccgg cacatggtcc
     2581 cgttcctgat actttgtgca cataccggca ccgggggaag ccccgaatcc cgtctggcgc
     2641 tgctggtaga cactcactcc cgtcaggttt tttgatttca agattgtagc agttttcagc
     2701 ccattcacca gcttcataat tgaccacctt tggtgggaca ccggacctga agccccatct
     2761 tttagtcgca gatggcacgt cagttgccac tccattcccc tcgagattca gtccaactga
     2821 tctcaattga tttgtggatg acagtttgtc acgacaaact agtttgtcga catcactaac
     2881 ctgtaatgta ctattgtgga taactccaag cgggatggaa aatgttcttt ggaaaaggat
     2941 aattacccaa agaaagaatg atgtcctctt gaatcgatca cgaggtaact gcaatattcc
     3001 tgtaacaccc atggtggctt taccaacagt accggattgc caagctggaa gtcgagcttc
     3061 cattatatac cctctagtgt ctaagcttgg ccgccgaggc cccaccgtac acgcctaccg
     3121 cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg
     3181 tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca
     3241 aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg
     3301 actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa
     3361 tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac
     3421 acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg
     3481 aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg
     3541 tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata
     3601 tatgggctat gaactaatga ccccgtaatt gattactatt aataactagt caataatcaa
     3661 tgtcaacgcg tatatctggc ggccagttag gccagagaaa tgttctggca cctgcacttg
     3721 cactggggac agcctatttt gctagtttgt tttgtttcgt tttgttttga tggagagcgt
     3781 atgttagtac tatcgattca cacaaaaaac caacacacag atgtaatgaa aataaagata
     3841 ttttattgcg gcctgtccaa tacctcccgt accttaatat tacttactta tccttgagag
     3901 acgtactagt aaccctgata aatgcttcaa taatattgaa aaaggacgag tatgagcatc
     3961 caacattttc gtgtcgcact cattcccttc tttgcggcat tttgcttgcc tgtttttgca
     4021 caccccgaaa cgctggtgaa agtaaaagat gctgaagatc aactgggtgc aagagtgggc
     4081 tatatcgaac tggatctcaa tagcggcaag atccttgagt ctttccgccc cgaagaacga
     4141 ttcccgatga tgagcacttt caaagtactg ctatgtggcg cggtgttgtc ccgtatagac
     4201 gccgggcaag agcagcttgg tcgccgtata cactactcac aaaacgactt ggttgagtac
     4261 tcgccggtca cggaaaagca tcttacggat ggcatgacgg taagagaatt gtgtagtgct
     4321 gccattacca tgagcgacaa caccgcggcc aacttacttc tgacaacgat cggaggccct
     4381 aaggagctga ctgcatttct tcataatatg ggtgatcatg tgacccggct tgaccgctgg
     4441 gaaccagagt tgaacgaagc cataccgaac gacgagcgtg ataccacgat gccagtagca
     4501 atggccacaa ctcttcggaa actactcact ggcgaacttc ttactctagc atcacgacag
     4561 cagctcatag actggatgga ggcggacaaa gtagcaggac cacttcttcg ctcggccctc
     4621 cctgctggct ggttcattgc tgacaaatcg ggggccggtg aacgcggctc tcgcggcatc
     4681 attgctgcgc tggggcctga tggtaagccc tcacgaatcg tagtgatcta cacgacgggg
     4741 agtcaggcca ctatggacga acgaaatagg cagatcgctg agatcggtgc ctcactgatc
     4801 aagcactggt aaccactgca gtggtttagc atttgcggcc gctgtcagac caagtttact
     4861 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     4921 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4981 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5041 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5101 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     5161 ttctagtgta gccgtagtta ggccaccact tcaagaac
//
