LOCUS       sequence-169931-         607 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 6xHis-tagged smo-1(GG), with a TEV protease cleavage site 
            between the tag and the ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Pelisch F, Sonneville R, Pourkarimi E, Agostinho A, Blow JJ, Gartner
            A, Hay RT
  TITLE     Dynamic SUMO modification regulates mitotic chromosome assembly and 
            cell cycle progression in Caenorhabditis elegans.
  JOURNAL   Nat Commun. 2014 Dec 5;5:5485. doi: 10.1038/ncomms6485.
  PUBMED    25475837
REFERENCE   2  (bases 1 to 607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-12-5"; volume: "5:5485. doi"; pages: " 
            10.1038/ncomms6485"
COMMENT     Sequence Label: 6xHis-TEV-SMO-1(GG)
FEATURES             Location/Qualifiers
     source          1..607
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..42
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        23..41
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    42..66
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             81..103
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             123..140
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             162..182
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             474..491
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(543..561)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      557..604
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 atcgatctcg atcccgcaaa attaatacga ctcactatag gggaattgtg agcggataac
       61 aattcccctc tagaaataat tttgtttaac tttaagaagg agatatacat atgtcgtact
      121 accatcacca tcaccatcac gattacgata tcccaacgac cgaaaacctg tattttcagg
      181 gcgccatggc cgatgatgca gctcaagcag gcgacaacgc cgaatacatc aagatcaagg
      241 tcgttggaca ggacagcaac gaagtgcact tccgtgtaaa gtatggaacc tctatggcca
      301 agctgaagaa gtcgtacgct gacagaaccg gtgtcgccgt caacagtctt cgtttcctct
      361 tcgacggacg tcgcatcaac gacgatgaca caccgaagac gctcgagatg gaggacgatg
      421 acgtcatcga ggtctaccaa gagcagctgg gcggatgagc ggccgcactc gagcaccacc
      481 accaccacca ctgagatccg gctgctaaca aagcccaaag gaagctgagt tggctgctgc
      541 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
      601 tttgctg
//
