LOCUS       sequence-169933-        1248 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 6xHis-tagged gei-17 (isoform f), with a TEV protease 
            cleavage site between the tag and the ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Pelisch F, Sonneville R, Pourkarimi E, Agostinho A, Blow JJ, Gartner
            A, Hay RT
  TITLE     Dynamic SUMO modification regulates mitotic chromosome assembly and 
            cell cycle progression in Caenorhabditis elegans.
  JOURNAL   Nat Commun. 2014 Dec 5;5:5485. doi: 10.1038/ncomms6485.
  PUBMED    25475837
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-12-5"; volume: "5:5485. doi"; pages: " 
            10.1038/ncomms6485"
COMMENT     Sequence Label: 6xHis-TEV-GEI-17(133-509)
FEATURES             Location/Qualifiers
     source          1..1248
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..33
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             52..72
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac cattacgata tcccaacgac cgaaaacctg
       61 tattttcagg gcgccggtgg cggtagccaa caaatgatgg ctagtcatca cagtcatttg
      121 cagcaacagc accatcctag caccccaaag aaaatgtacg ccgacaactt tgagcctttg
      181 ccacttccct tttacgacgt tatctcggtc ttgttaaaac ctgtagagtt gcattcgtcc
      241 gactcgccga cccttaaaca aacgaaacag ttacagtttc catttctgct gacagcggaa
      301 catatttcca aaatctctta tcgcgccgat gtcacacctt tgccgcgcta cgagttacag
      361 cttcgctttt tcaacttaac ggaaccggtc caaggaccgc aaaaggatga ttttccgctg
      421 aactgttacg cccgtgtcga cgactctgta gttcagttgc ccaatgtaat tcccacaaac
      481 aaaacaaacg cagagcccaa gcgtccatcc cgtccagtca acattacgag caatatgaat
      541 cgctacaaga aagaacatac cgttgcggta gaatggttag ctgacaaacg cgtgtgggct
      601 gctggtgtat actttgtaca ccgcgtcaac tccgatatcc tgttcaagcg ccttaaccag
      661 aatgtttcac gtcaccgctc gcttgaggta acaaagcagg aagtaattaa gaagctgtca
      721 gggggcgagg acgatatcgc tatggaccgt cttaatatct ctttattaga cccgctgtgt
      781 aagactcgta tgacgacacc gtcgcgctgt caagactgta cccatttgca atgcttcgac
      841 ttactgtcct acctgatgat gaacgaaaaa aaaccaactt ggcaatgccc tgtgtgtagc
      901 tccaattgtc cgtacgatcg tttgattgtc gacgactatt ttttggatat gcttgcaaaa
      961 gtagacaaaa atactaccga agttgaactg aaagaggacg ggtcctacga tgtaatcaaa
     1021 gaggaggctt tttgtatctc agatgacgat gatgacgacg ttgtgccggc cacagttaat
     1081 gggacggcct catgctccag tacgaacggg aacgggctgg ccaatgaagc tgctaaaaaa
     1141 aagcccgcag acgacgacat tattactctt tcggatgacg atgatgaaga attgaatcgt
     1201 ggcatcatga attcgttgta gcgagctccg tcgacaagct tgcggccg
//
