LOCUS       sequence-169947-       13786 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MESA target chain with V2-MESA ectodomain, 35 extracellular linkers,
            a flag tag, M cleavage sequence, and dCas9-VP64.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13786)
  AUTHORS   Schwarz KA, Daringer NM, Dolberg TB, Leonard JN
  TITLE     Rewiring human cellular input-output using modular extracellular 
            sensors.
  JOURNAL   Nat Chem Biol. 2016 Dec 12. doi: 10.1038/nchembio.2253.
  PUBMED    27941759
REFERENCE   2  (bases 1 to 13786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Dec 12. doi: 10.1038/nchembio.2253."
COMMENT     Sequence Label: V2-MESA-35F-M-dCas9
FEATURES             Location/Qualifiers
     source          1..13786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(75..94)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        266..645
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        647..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     800..820
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             863..1043
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1090..1215
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1708..1941
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2126..2170
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2468..2585
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             3574..3594
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQM"
     CDS             3610..3657
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             join(3658..3681,3682..3684,3685..6240,6241..6243,
                     6244..7758)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7813..7833
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7858..8007
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             8029..8082
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8083..8481
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8506..9094
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8559..8579)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8977..8988)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9097..9113)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9098..9114)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9619..9799
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9825..9842)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9831..10055
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10101..10529
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10188..10207)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10398..10419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10538..10558)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10543..10872
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10723..10858
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10785..10804
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10920..10967
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10986..11360
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11490..11611
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11527..11546)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11581..11600
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11660..11676)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11660..11676)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11673..11695)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11684..11700
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11708..11714,11715..11732,11733..11738))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11753..11774
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11891..11908)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12062..12650)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12142..12161)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12821..13612,13613..13681))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13444..13463
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13682..13786)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
       61 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
      121 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
      181 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
      241 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
      301 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      361 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      421 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      481 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      541 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      601 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      661 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      721 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      781 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      841 agcagcgcgt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
      901 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
      961 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     1021 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     1081 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     1141 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     1201 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     1261 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     1321 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     1381 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     1441 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     1501 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     1561 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     1621 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     1681 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     1741 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     1801 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     1861 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     1921 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     1981 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     2041 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     2101 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     2161 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     2221 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     2281 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     2341 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     2401 tcgattagtg aacggatcgg cactgcgtgc gccaattctg cagacaaatg gcagtattca
     2461 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     2521 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     2581 attttcgggt ttattacagg gacagcagag atccagtttg gttaattagc tagcgccatc
     2641 atggcttgga ccagcttaat tcttagcttg cttgccctgt gcagcggtgc atcatctgac
     2701 atacagatga ctcagtcacc tagcagtctg tctgcttcag tcggtgatag ggtaactatc
     2761 acctgtcggg catctcaggt gatcagaagg tctctggcct ggtatcagca gaagcccggc
     2821 aaggctccta agttgcttat ctatgcagct tcaaacttgg ccagtggggt gcctagccgg
     2881 tttagcggaa gtggttctgg cacagacttc actttgacaa tcagttccct ccaaccggag
     2941 gatttcgcta cctactactg tcagcagtcc aacaccagcc ctctgacgtt tggacagggc
     3001 actaaagtgg agattaagag gggaggcgga ggctccggtg ggggaggctc tggtggtgga
     3061 ggtagcgagg ttcagctggt ggaaagtggt ggcggactag tacagccagg cggctccctg
     3121 cggctgagtt gcgccgcgag tggcttttct ataaacggat cctggatctt ctgggtcagg
     3181 caagcccctg ggaaaggatt ggagtgggtt ggagccattt ggcctttcgg tgggtatact
     3241 cactatgcag atagcgtgaa agggaggttc accatctctg cagacacaag caagaacacg
     3301 gcctacctac agatgaactc tctgagggca gaggacactg ccgtctacta ttgcgcccga
     3361 tggggacact ccacaagccc ttgggccatg gactattggg gacaggggac attggtaggc
     3421 gccggatctg gaggagacta caaggacgat gacgacaacg gcggatcggg ggggtctggt
     3481 ggtagtggcg gttcgggggg aggtaccggt ctggtcgtgg ttgctggagt cctgttttgt
     3541 tatggcttgc tagtgacagt ggctctttgt gttgaaaacc tgtattttca gatgcgtacg
     3601 gccaccatga aaaggccggc ggccacgaaa aaggccggcc aggcaaaaaa gaaaaaggac
     3661 aagaagtaca gcatcggcct ggccatcggc accaactctg tgggctgggc cgtgatcacc
     3721 gacgagtaca aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc
     3781 atcaagaaga acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc
     3841 cggctgaaga gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg
     3901 caagagatct tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa
     3961 gagtccttcc tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc
     4021 gtggacgagg tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg
     4081 gtggacagca ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc
     4141 aagttccggg gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac
     4201 aagctgttca tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac
     4261 gccagcggcg tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg
     4321 gaaaatctga tcgcccagct gcccggcgag aagaagaatg gcctgttcgg caacctgatt
     4381 gccctgagcc tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc
     4441 aaactgcagc tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc
     4501 ggcgaccagt acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg
     4561 agcgacatcc tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc
     4621 aagagatacg acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag
     4681 ctgcctgaga agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac
     4741 attgacggcg gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag
     4801 atggacggca ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag
     4861 cggaccttcg acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt
     4921 ctgcggcggc aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag
     4981 atcctgacct tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc
     5041 gcctggatga ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg
     5101 gacaagggcg cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg
     5161 cccaacgaga aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac
     5221 gagctgacca aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc
     5281 gagcagaaaa aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag
     5341 cagctgaaag aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc
     5401 gtggaagatc ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag
     5461 gacaaggact tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc
     5521 ctgacactgt ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg
     5581 ttcgacgaca aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg
     5641 agccggaagc tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc
     5701 ctgaagtccg acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg
     5761 acctttaaag aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag
     5821 cacattgcca atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag
     5881 gtggtggacg agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa
     5941 atggccagag agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag
     6001 cggatcgaag agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa
     6061 aacacccagc tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg
     6121 tacgtggacc aggaactgga catcaaccgg ctgtccgact acgatgtgga ccacatcgtg
     6181 cctcagagct ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag
     6241 gcccggggca agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac
     6301 tggcggcagc tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag
     6361 gccgagagag gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg
     6421 gaaacccggc agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag
     6481 tacgacgaga atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg
     6541 gtgtccgatt tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac
     6601 cacgcccacg acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct
     6661 aagctggaaa gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc
     6721 gccaagagcg agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc
     6781 atgaactttt tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg
     6841 atcgagacaa acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc
     6901 gtgcggaaag tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca
     6961 ggcggcttca gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga
     7021 aagaaggact gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct
     7081 gtgctggtgg tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag
     7141 ctgctgggga tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg
     7201 gaagccaagg gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc
     7261 ctgttcgagc tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag
     7321 ggaaacgaac tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat
     7381 gagaagctga agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac
     7441 aagcactacc tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg
     7501 gccgacgcta atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc
     7561 agagagcagg ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc
     7621 gccttcaagt actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg
     7681 ctggacgcca ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg
     7741 tctcagctgg gaggcgacag cgctggagga ggtggaagcg gaggaggagg aagcggagga
     7801 ggaggtagcg gacctaagaa aaagaggaag gtggcggccg ctggatccgg acgggctgac
     7861 gcattggacg attttgatct ggatatgctg ggaagtgacg ccctcgatga ttttgacctt
     7921 gacatgcttg gttcggatgc ccttgatgac tttgacctcg acatgctcgg cagtgacgcc
     7981 cttgatgatt tcgacctgga catgctgatt aactgtacag gcagtggaga gggcagagga
     8041 agtctgctaa catgcggtga cgtcgaggag aatcctggcc caatggccaa gcctttgtct
     8101 caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct
     8161 gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc
     8221 aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct
     8281 gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc
     8341 ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc
     8401 atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct
     8461 ggttatgtgt gggagggcta agaattcgat atcaagctta tcggtaatca acctctggat
     8521 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     8581 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     8641 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     8701 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     8761 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     8821 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     8881 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     8941 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     9001 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     9061 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta
     9121 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
     9181 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
     9241 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     9301 gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca
     9361 caaggctact tccctgattg gcagaactac acaccagggc cagggatcag atatccactg
     9421 acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat
     9481 gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag
     9541 agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag
     9601 ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     9661 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     9721 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     9781 gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
     9841 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     9901 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     9961 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
    10021 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
    10081 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
    10141 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
    10201 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
    10261 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
    10321 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
    10381 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
    10441 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
    10501 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
    10561 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
    10621 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
    10681 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
    10741 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
    10801 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
    10861 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg
    10921 ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact
    10981 aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag
    11041 cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg
    11101 ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc
    11161 cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt
    11221 cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg
    11281 agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg
    11341 tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg
    11401 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
    11461 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
    11521 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
    11581 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
    11641 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
    11701 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
    11761 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
    11821 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
    11881 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
    11941 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
    12001 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
    12061 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
    12121 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    12181 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    12241 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    12301 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    12361 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    12421 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    12481 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
    12541 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    12601 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    12661 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    12721 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    12781 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    12841 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    12901 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    12961 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    13021 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    13081 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    13141 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    13201 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    13261 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    13321 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    13381 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    13441 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    13501 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    13561 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    13621 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    13681 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    13741 acatatttga atgtatttag aaaaataaac aaataggggt tccgcg
//
