LOCUS       sequence-169952-         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mCherry-tagged human Akt2(K14A) membrane binding mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Ebner M, Lucic I, Leonard TA, Yudushkin I
  TITLE     PI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes.
  JOURNAL   Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 
            10.1016/j.molcel.2016.12.028.
  PUBMED    28157504
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.12"; journalName: "Mol Cell"; date: 
            "2017-02-2- 2"; volume: "65"; issue: "3"; pages: "416-431.e6"
COMMENT     Sequence Label: pmCherry-Akt2(K14A)
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             46..753
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(191..209)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     459..478
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc
       61 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
      121 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      181 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
      241 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
      301 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      361 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
      421 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
      481 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
      541 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
      601 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
      661 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
      721 cactccaccg gcggcatgga cgagctgtac aagtccggac tcagatctcg agctatgaat
      781 gaggtgtctg tcatcaaaga aggctg
//
