LOCUS       sequence-169964-        8218 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express wild-type NSD2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8218)
  AUTHORS   Jaffe JD, Wang Y, Chan HM, Zhang J, Huether R, Kryukov GV, Bhang HE,
            Taylor JE, Hu M, Englund NP, Yan F, Wang Z, Robert McDonald E 3rd, 
            Wei L, Ma J, Easton J, Yu Z, deBeaumount R, Gibaja V, Venkatesan K, 
            Schlegel R, Sellers WR, Keen N, Liu J, Caponigro G, Barretina J, 
            Cooke VG, Mullighan C, Carr SA, Downing JR, Garraway LA, Stegmeier F
  TITLE     Global chromatin profiling reveals NSD2 mutations in pediatric acute
            lymphoblastic leukemia.
  JOURNAL   Nat Genet. 2013 Nov;45(11):1386-91. doi: 10.1038/ng.2777. Epub 2013 
            Sep 29.
  PUBMED    24076604
REFERENCE   2  (bases 1 to 8218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.2777"; 
            journalName: "Nat Genet"; date: "2013-11"; volume: "45"; issue: 
            "11"; pages: "1386-91"
COMMENT     Sequence Label: LVXN-Neo-NSD2
FEATURES             Location/Qualifiers
     source          1..8218
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             125..758
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    805..930
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1427..1660
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1845..1889
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2152..2267
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2325..2628
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2629..2832
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2783..2803
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2829..2853
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    3027..3489
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3083..3100)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3310..3329
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3503..4297
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3557..3576)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4167..4186
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    4311..4899
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4364..4384)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4782..4793)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5106..5739
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5867..5883)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5867..5883)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5880..5902)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5891..5907
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5915..5921,5922..5939,5940..5945))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5960..5981
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6098..6115)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6269..6857)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6349..6368)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7028..7819,7820..7888))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7651..7670
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7889..7993)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8041..8175
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8078..8097)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8132..8151
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
       61 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
      121 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
      181 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
      241 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
      301 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
      361 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
      421 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
      481 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
      541 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
      601 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      661 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      721 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
      781 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
      841 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
      901 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
      961 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     1021 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     1081 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1141 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1201 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1261 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     1321 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1381 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     1441 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     1501 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     1561 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     1621 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     1681 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     1741 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     1801 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     1861 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     1921 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     1981 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     2041 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     2101 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc ctttaaaaga
     2161 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2221 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     2281 agggacagca gagatccagt ttatcgataa gcttgggagt tccgcgttac ataacttacg
     2341 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     2401 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     2461 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     2521 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     2581 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     2641 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     2701 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     2761 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     2821 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     2881 gacctccata gaagacaccg actctactag aggatctatt tccggtgaat tccccctaac
     2941 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     3001 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     3061 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     3121 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     3181 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     3241 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     3301 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     3361 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     3421 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     3481 cgatgataag cttgccacaa ccatggctga acaagatgga ttgcacgcag gttctccggc
     3541 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     3601 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     3661 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     3721 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     3781 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     3841 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     3901 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     3961 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     4021 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     4081 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     4141 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     4201 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     4261 catcgccttc tatcgccttc ttgacgagtt cttctgaacg cgtctggaac aatcaacctc
     4321 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     4381 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     4441 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     4501 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     4561 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     4621 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     4681 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     4741 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     4801 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     4861 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg
     4921 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     4981 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     5041 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag
     5101 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     5161 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     5221 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     5281 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     5341 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     5401 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     5461 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     5521 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     5581 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     5641 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     5701 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     5761 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt
     5821 gctagcgttt accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     5881 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     5941 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     6001 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     6061 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6121 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6181 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6241 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6301 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6361 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6421 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6481 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6541 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     6601 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     6661 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     6721 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     6781 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     6841 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     6901 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     6961 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7021 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7081 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7141 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7201 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7261 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7321 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7381 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7441 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7501 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7561 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     7621 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     7681 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     7741 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     7801 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     7861 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     7921 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     7981 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     8041 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     8101 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     8161 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttccca
//
