LOCUS       sequence-169965-        9244 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express NSD2 E1099K mutant  in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9244)
  AUTHORS   Jaffe JD, Wang Y, Chan HM, Zhang J, Huether R, Kryukov GV, Bhang HE,
            Taylor JE, Hu M, Englund NP, Yan F, Wang Z, Robert McDonald E 3rd, 
            Wei L, Ma J, Easton J, Yu Z, deBeaumount R, Gibaja V, Venkatesan K, 
            Schlegel R, Sellers WR, Keen N, Liu J, Caponigro G, Barretina J, 
            Cooke VG, Mullighan C, Carr SA, Downing JR, Garraway LA, Stegmeier F
  TITLE     Global chromatin profiling reveals NSD2 mutations in pediatric acute
            lymphoblastic leukemia.
  JOURNAL   Nat Genet. 2013 Nov;45(11):1386-91. doi: 10.1038/ng.2777. Epub 2013 
            Sep 29.
  PUBMED    24076604
REFERENCE   2  (bases 1 to 9244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.2777"; 
            journalName: "Nat Genet"; date: "2013-11"; volume: "45"; issue: 
            "11"; pages: "1386-91"
COMMENT     Sequence Label: LVXN-Neo-NSD2-E1099K
FEATURES             Location/Qualifiers
     source          1..9244
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     135..154
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             328..1122
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(382..401)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     992..1011
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    1136..1724
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1189..1209)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1607..1618)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1931..2564
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2611..2736
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3233..3466
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3651..3695
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3958..4073
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        4131..4434
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4435..4638
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4589..4609
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4635..4659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4743..4757
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     protein_bind    4760..4784
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4793..4802
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4802..4825
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7504..7515
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(8947..8971)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(9152..9169)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
       61 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
      121 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
      181 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
      241 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
      301 aaacacgatg ataagcttgc cacaaccatg gctgaacaag atggattgca cgcaggttct
      361 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
      421 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
      481 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
      541 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
      601 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
      661 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
      721 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
      781 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
      841 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
      901 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
      961 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     1021 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     1081 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaacgcgtct ggaacaatca
     1141 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     1201 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     1261 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     1321 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     1381 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     1441 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     1501 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     1561 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     1621 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     1681 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc
     1741 agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     1801 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
     1861 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     1921 aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg
     1981 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
     2041 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
     2101 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
     2161 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
     2221 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
     2281 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
     2341 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga
     2401 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     2461 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     2521 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     2581 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     2641 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     2701 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     2761 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     2821 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     2881 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     2941 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     3001 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     3061 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     3121 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     3181 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     3241 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     3301 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     3361 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     3421 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     3481 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     3541 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     3601 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     3661 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     3721 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     3781 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     3841 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     3901 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt
     3961 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     4021 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     4081 tattacaggg acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa
     4141 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     4201 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     4261 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     4321 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     4381 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     4441 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     4501 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     4561 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     4621 gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
     4681 tgttttgacc tccatagaag acaccgactc tactagagga tctatttccg gtgaattgta
     4741 ccgacgacga cgacaaggca caagtttgta caaaaaagca ggctccgcgg ccgccaccat
     4801 ggactacaag gacgacgatg acaaaggtgg ttctggagga tcagaattta gcatcaagca
     4861 gagtcccctt tctgttcaga gtgttgtaaa gtgcataaag atgaagcagg caccagaaat
     4921 cctcggcagt gccaacggga agactccgag ctgcgaggtg aaccgcgagt gttctgtgtt
     4981 cctcagcaaa gcccagctct ccagtagcct gcaggagggg gtcatgcaga agtttaacgg
     5041 ccacgacgcc ctgcccttta ttccagccga caagctgaaa gatcttactt cccgggtgtt
     5101 taatggagaa cccggcgcac acgatgccaa actgcgtttt gagtcccagg aaatgaaagg
     5161 gattgggaca ccccctaaca ctacccctat caaaaatggc tctccagaaa ttaagctgaa
     5221 aatcaccaaa acatacatga atgggaagcc tctctttgaa tcttccattt gtggtgacag
     5281 tgctgctgat gtgtctcagt cagaagaaaa tggacaaaaa ccagaaaaca aggcgagaag
     5341 gaacaggaag aggagcataa aatatgactc cttgctggag cagggccttg tcgaagcagc
     5401 tcttgtgtct aagatctcaa gtccttcaga taaaaagatt ccagctaaga aagagtcttg
     5461 tccaaacact ggaagagaca aagaccacct gttgaaatac aacgttggtg atttggtgtg
     5521 gtccaaagtg tcgggttacc cttggtggcc ttgcatggtt tctgcagatc cactccttca
     5581 cagctatacc aaacttaaag gtcagaaaaa gagtgcacgc cagtatcacg tacagttctt
     5641 tggtgacgcc ccagaaagag cttggatatt tgagaagagc ctcgtagctt ttgaaggaga
     5701 aggacagttt gaaaaattat gccaggaaag tgccaagcag gcacccacga aagctgagaa
     5761 aattaagcta ttgaaaccaa tttcagggaa attgagggcc cagtgggaaa tgggcattgt
     5821 tcaagcagaa gaagctgcaa gcatgtcagt ggaggagcgg aaagccaagt tcacctttct
     5881 ctatgtgggg gaccagcttc atctcaaccc tcaagtagcc aaggaggctg gcattgctgc
     5941 agagtctttg ggagaaatgg cagaatcctc aggagtcagt gaagaagctg ctgaaaaccc
     6001 caagtctgtg agagaagagt gcattcccat gaagagaagg cggagggcca aactgtgtag
     6061 ctctgcagag accctggaga gtcaccccga catagggaag agtactcctc aaaagacggc
     6121 agaggctgac cccagaagag gagtagggtc tcctcctggg aggaagaaga ccacagtctc
     6181 catgccacga agcaggaagg gagatgcagc atcccagttt ttggtcttct gtcaaaaaca
     6241 cagggatgag gtggtagctg agcacccaga tgcttcaggt gaggagattg aagagctgct
     6301 caggtcacag tggagtctgc tgagtgagaa gcagagagca cgctacaaca ccaagtttgc
     6361 cctggtggcc cctgtccagg ctgaagaaga ctctggtaat gtaaatggga aaaaaagaaa
     6421 ccacacaaag aggatacagg accctacaga agatgctgaa gctgaggaca cacccaggaa
     6481 aagactcagg acggacaagc acagtcttcg gaagagagac acaatcactg acaaaacggc
     6541 cagaacaagc tcttacaagg ccatggaggc agcctcctcg ctcaagagcc aggcagcaac
     6601 gaaaaatctg tctgatgcat gtaaaccact gaagaagcga aatcgggctt ccacggcagc
     6661 atcttcagct cttgggttta gcaaaagttc atctccttct gcatccttaa ctgagaatga
     6721 ggtctcggac agcccgggag acgagccctc ggagtcccca tacgaaagtg cagacgaaac
     6781 acaaactgaa gtatctgtct catccaaaaa gtctgagcga ggagtgactg ccaaaaagga
     6841 gtatgtgtgc cagctgtgtg agaagccggg cagcctcctg ctctgtgaag gaccctgctg
     6901 cggagctttc cacctcgcct gccttgggct ttcccggagg ccagaaggga ggttcacctg
     6961 cagcgagtgt gcctcaggga ttcactcatg tttcgtgtgt aaagagagca agacagatgt
     7021 taagcgctgt gtggtaactc agtgtggaaa attttaccat gaggcttgtg tgaaaaaata
     7081 ccctctgact gtatttgaga gccgaggttt ccgctgcccc ctccacagct gtgtgagctg
     7141 ccatgcttcc aacccttcaa acccaaggcc gtcaaaaggt aaaatgatgc ggtgtgtccg
     7201 ctgccccgtt gcctatcaca gcggggatgc ttgtctggca gcaggatgct cagtgatcgc
     7261 ctccaacagc atcatctgca ctgcccactt cactgctcgg aaggggaagc gacaccacgc
     7321 ccacgtcaac gtgagctggt gcttcgtgtg ctccaaaggg gggagccttc tgtgctgtga
     7381 gtcctgccca gcggccttcc accctgactg cctgaacatc gagatgcctg acggcagctg
     7441 gttctgcaat gactgcaggg ctgggaagaa gctgcacttc caggatatca tttgggtgaa
     7501 acttgggaac tacagatggt ggccggcaga agtttgccat cccaaaaatg ttcccccaaa
     7561 tattcagaaa atgaagcacg agattggaga attccctgtg tttttctttg ggtctaaaga
     7621 ttattactgg acgcatcagg cgcgagtgtt cccgtacatg gagggggacc ggggcagccg
     7681 ctaccagggg gtcagaggga tcggaagagt cttcaaaaac gcactgcaag aagctgaagc
     7741 tcgttttcgt gaaattaagc ttcagaggga agcccgagaa acacaggaga gcgagcgcaa
     7801 gcccccacca tacaagcaca tcaaggtcaa caaaccctat ggccgcgtcc agatctacac
     7861 agcggatatt tcagaaatcc ctaagtgcaa ctgcaagccc acagatgaga atccttgtgg
     7921 ctttgattcg gagtgtctga acaggatgct gatgtttgag tgccacccgc aggtgtgtcc
     7981 cgcgggcgag ttctgccaga accagtgctt caccaagcgc cagtacccag agaccaagat
     8041 catcaagaca gatggcaaag ggtggggcct ggtcgccaag agggacatca gaaagggaga
     8101 atttgttaac gagtacgttg gggagctgat cgacaaggag gagtgcatgg cgagaatcaa
     8161 gcacgcacac gagaacgaca tcacccactt ctacatgctc actatagaca aggaccgtat
     8221 aatagacgct ggccccaaag gaaactactc tcgatttatg aatcacagct gccagcccaa
     8281 ctgtgagacc ctcaagtgga cagtgaatgg ggacactcgt gtgggcctgt ttgccgtctg
     8341 tgacattcct gcagggacgg agctgacttt taactacaac ctcgattgtc tgggcaatga
     8401 aaaaacggtc tgccgatgtg gagcctccaa ttgcagtgga ttcctcgggg atagaccaaa
     8461 gacctcgacg accctttcat cagaggaaaa gggcaaaaag accaagaaga aaacgaggcg
     8521 gcgcagagca aaaggggaag ggaagaggca gtcagaggac gagtgcttcc gctgcggtga
     8581 tggcgggcag ctggtgctgt gtgaccgcaa gttctgcacc aaggcctacc acctgtcctg
     8641 cctgggcctt ggcaagcggc ccttcgggaa gtgggaatgt ccttggcatc attgtgacgt
     8701 gtgtgggaag ccctctacct ctttctgcca cctctgcccc aattcgttct gtaaggagca
     8761 ccaggacggg acagccttca gctgcacccc ggacgggcgg tcctactgct gtgagcatga
     8821 cttaggggcg gcatcggtca gaagcaccaa gactgagaag ccccccccag agccagggaa
     8881 gccgaagggg aagaggcggc ggcggagggg ctggcggaga gtcacagagg gcaaataggc
     8941 gcgccgaccc agctttcttg tacaaagtgg ttgcgatgat cccgcccctc tccctccccc
     9001 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     9061 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     9121 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     9181 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     9241 accc
//
