LOCUS       sequence-169966-        3237 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express NSD2 E1099K mutant  in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Jaffe JD, Wang Y, Chan HM, Zhang J, Huether R, Kryukov GV, Bhang HE,
            Taylor JE, Hu M, Englund NP, Yan F, Wang Z, Robert McDonald E 3rd, 
            Wei L, Ma J, Easton J, Yu Z, deBeaumount R, Gibaja V, Venkatesan K, 
            Schlegel R, Sellers WR, Keen N, Liu J, Caponigro G, Barretina J, 
            Cooke VG, Mullighan C, Carr SA, Downing JR, Garraway LA, Stegmeier F
  TITLE     Global chromatin profiling reveals NSD2 mutations in pediatric acute
            lymphoblastic leukemia.
  JOURNAL   Nat Genet. 2013 Nov;45(11):1386-91. doi: 10.1038/ng.2777. Epub 2013 
            Sep 29.
  PUBMED    24076604
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.2777"; 
            journalName: "Nat Genet"; date: "2013-11"; volume: "45"; issue: 
            "11"; pages: "1386-91"
COMMENT     Sequence Label: LVXN-Neo-NSD2-E1099K
FEATURES             Location/Qualifiers
     source          1..3237
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             52..309
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(437..453)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(437..453)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(450..472)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    461..477
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(485..491,492..509,510..515))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    530..551
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(668..685)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(839..1427)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(919..938)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1598..2389,2390..2458))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2221..2240
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2459..2563)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    2611..2745
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2648..2667)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2702..2721
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg
       61 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg
      121 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
      181 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
      241 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
      301 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
      361 gaatatcaga gagtgagagg ccttgacatt gctagcgttt accgtcgacc tctagctaga
      421 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
      481 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
      541 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
      601 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
      661 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
      721 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
      781 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      841 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      901 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      961 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1021 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1081 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1141 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1201 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1261 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1321 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1381 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1441 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1501 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     1561 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     1621 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     1681 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     1741 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     1801 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     1861 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     1921 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     1981 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     2041 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     2101 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     2161 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     2221 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     2281 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     2341 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     2401 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     2461 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     2521 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     2581 tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac
     2641 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     2701 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
     2761 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
     2821 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
     2881 atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag
     2941 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa
     3001 ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt
     3061 accagttgag ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca
     3121 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga
     3181 cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaa
//
