LOCUS       sequence-169977-        9463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9463)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 9463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: 
            AB.pCCL.sin.cPPT.GFP.miR-370-5p.sensor.PGK.dNGFR.WPRE
FEATURES             Location/Qualifiers
     source          1..9463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..3,4..6,7..720)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    996..1130
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    1267..1384
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1651..1695)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1880..2113
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2606..2731
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2778..2958
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2973..3176)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        3177..3556
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(3657..3677)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3657..3675)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3696..3712)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3696..3712)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3709..3731)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3720..3736
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3744..3750,3751..3768,3769..3774))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3789..3810
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3927..3944)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4098..4686)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4178..4197)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4857..5648,5649..5717))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5480..5499
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5718..5822)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5848..6303)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5985..6006)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6197..6216
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6430..6452
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6444..6461
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6445..6461
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6471..6490
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6471..6489
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      6510..6645
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6685..6806
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             6878..7111
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    7198..7786
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7304..7315
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7713..7733
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     promoter        complement(8790..9300)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8853..8871)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        9328..9366
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
      721 agcggccgcg tcgagagatg ggggaggcta actgagctag ccgaccttaa ctggggagta
      781 ctggtcgacg tagcgtaact gcagagacgt gacctggtaa ctgcagagac gtgacctggt
      841 aactgcagag acgtgacctg gtaactgcag agacgtgacc tgtaactgca gagacgtgac
      901 ctggtaactg cagagacgtg acctgaccgg tactagctta gtcatgcacc gggctgcagg
      961 aattcgatga caggcgcagc ttccgaggga tttgagatcc agacatgata agatacattg
     1021 atgagtttgg acaaaccaaa actagaatgc agtgaaaaaa atgccttatt tgtgaaattt
     1081 gtgatgctat tgccttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     1141 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     1201 aaaacctcta caaatgtggt atggctgatt atgatcagat ctctcgaggc tagtctcgtg
     1261 atcgataaaa ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt
     1321 tgctattatg tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt
     1381 aaaagttaac cgataccgtc gagatccgtt cactaatcga atggatctgt ctctgtctct
     1441 ctctccacct tcttcttcta ttccttcggg cctgtcgggt cccctcgggg ttgggaggtg
     1501 ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag aaagtacagc
     1561 aaaaactatt cttaaaccta ccaagcctcc tactatcatt atgaataatt ttatatacca
     1621 cagccaattt gttatgttaa accaattcca caaacttgcc catttatcta attccaataa
     1681 ttcttgttca ttcttttctt gctggttttg cgattcttca attaaggagt gtattaagct
     1741 tgtgtaattg ttaatttctc tgtcccactc catccaggtc gtgtgattcc aaatctgttc
     1801 cagagattta ttactccaac tagcattcca aggcacagca gtggtgcaaa tgagttttcc
     1861 agagcaaccc caaatcccca ggagctgttg atcctttagg tatctttcca cagccaggat
     1921 tcttgcctgg agctgcttga tgccccagac tgtgagttgc aacagatgct gttgcgcctc
     1981 aatagccctc agcaaattgt tctgctgctg cactatacca gacaataatt gtctggcctg
     2041 taccgtcagc gtcattgacg ctgcgcccat agtgcttcct gctgctccca agaacccaag
     2101 gaacaaagct cctattccca ctgctctttt ttctctctgc accactcttc tctttgcctt
     2161 ggtgggtgct actcctaatg gttcaatttt tactacttta tatttatata attcacttct
     2221 ccaattgtcc ctcatatctc ctcctccagg tctgaagatc agcggccgct tgctgtgcgg
     2281 tggtcttact tttgttttgc tcttcctcta tcttgtctaa agcttccttg gtgtctttta
     2341 tctctatcct ttgatgcaca caatagaggg ttgctactgt attatataat gatctaagtt
     2401 cttctgatcc tgtctgaagg gatggttgta gctgtcccag tatttgtcta cagccttctg
     2461 atgtttctaa caggccagga ttaactgcga atcgttctag ctccctgctt gcccatacta
     2521 tatgttttaa tttatatttt ttctttcccc ctggccttaa ccgaattttt tcccatcgcg
     2581 atctaattct cccccgctta atactgacgc tctcgcaccc atctctctcc ttctagcctc
     2641 cgctagtcaa aatttttggc gtactcacca gtcgccgccc ctcgcctctt gccgtgcgcg
     2701 cttcagcaag ccgagtcctg cgtcgagaga gctcctctgg tttccctttc gctttcaagt
     2761 ccctgttcgg gcgccactgc tagagatttt ccacactgac taaaagggtc tgagggatct
     2821 ctagttacca gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct
     2881 ttattgaggc ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg
     2941 gtctaaccag agagaccccg gttcactaaa cgagctctgc ttatatagac ctcccaccgt
     3001 acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc
     3061 cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat
     3121 ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg
     3181 gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg
     3241 tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt
     3301 ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat
     3361 tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc
     3421 aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg
     3481 cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta
     3541 gtcaataatc aatgtcaaca tggcggtaat gttggacatg agccaatata aatgtacata
     3601 ttatgatatg gatacaacgt atgcaatggc caagcttgca gctccagctt ttgttccctt
     3661 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     3721 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     3781 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     3841 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     3901 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     3961 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     4021 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     4081 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     4141 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4201 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4261 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4321 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4381 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     4441 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     4501 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     4561 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     4621 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4681 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4741 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     4801 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4861 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     4921 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     4981 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     5041 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     5101 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     5161 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     5221 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     5281 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     5341 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     5401 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     5461 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     5521 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     5581 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     5641 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     5701 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     5761 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     5821 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg
     5881 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     5941 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     6001 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     6061 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     6121 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     6181 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     6241 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     6301 cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     6361 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     6421 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact
     6481 cactataggg cgaattgggt acgtccctag gcctccaaaa aagcctcctc actacttctg
     6541 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     6601 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     6661 cgggatagct agagccagac atgataagat acattgatga gtttggacaa accacaacta
     6721 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     6781 ccattataag ctgcaataaa caagttcctc tcactctctg atattcattt ctttgcaagt
     6841 tataaatact gaataataag atgacatgaa ctactactgc tagagatttt ccacactgac
     6901 taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc acacactact
     6961 tgaagcactc aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag
     7021 agctcccagg ctcagatctg gtctaaccag agagacccag tacaagcaaa aagcagatct
     7081 tgtcttcgtt gggagtgaat tagcccttcc agtcccccct tttcttttaa aaagtggcta
     7141 agatctacag ctgccttgta agtcattggt cttaaaggta ccgagctcga attccaggcg
     7201 gggaggcggc ccaaagggag atccgactcg tctgagggcg aaggcgaaga cgcggaagag
     7261 gccgcagagc cggcagcagg ccgcgggaag gaaggtccgc tggattgagg gccgaaggga
     7321 cgtagcagaa ggacgtcccg cgcagaatcc aggtggcaac acaggcgagc agccatggaa
     7381 aggacgtcag cttccccgac aacaccacgg aattgtcagt gcccaacagc cgagcccctg
     7441 tccagcagcg ggcaaggcag gcggcgatga gttccgccgt ggcaataggg agggggaaag
     7501 cgaaagtccc ggaaaggagc tgacaggtgg tggcaatgcc ccaaccagtg ggggttgcgt
     7561 cagcaaacac agtgcacacc acgccacgtt gcctgacaac gggccacaac tcctcataaa
     7621 gagacagcaa ccaggattta tacaaggagg agaaaatgaa agccatacgg gaagcaatag
     7681 catgatacaa aggcattaaa gcagcgtatc cacatagcgt aaaaggagca acatagttaa
     7741 gaataccagt caatctttca caaattttgt aatccagagg ttgattgtcg actctagact
     7801 cgactctaga ggatccccct gttccacctc ttgaaggcta tgtaggccac aaggcccaca
     7861 accacagcag ccaggatgga gcaatagaca gggatgaggt tgtcggtggt gcctcgggtc
     7921 accacgggct gggagctgcc catcactgtg gtcaccacac ctgccaccgt gctggctatg
     7981 aggtcttgtt ctggaggtgc ctcaggctcc tgggtgctgg gggctgtgct gtccgagccc
     8041 tctgggggtg tggaccgtgt aatccaacgg ccagggatct cctcgcactc ggcgtcggcc
     8101 cagcgtgtgc actcgcggag ctggcgctcg gtgtcctcgc acacggtgca gggcaggcac
     8161 gggtccacgt ggttggcctc gtcggaatac gtgccgtcgg ggcactcctc gcacacggtg
     8221 ttctgcttgt cctggcagga gaacacgagg cccgagcccg cctcgcacac gcggcacgcc
     8281 tcgcagcgcc cagtcgtctc atcctggtag tagccgtagg cgcagcggca cacggcgtcg
     8341 tcggcctcca cgcacggcgc cgacatgctc tggagcccca cgcactcggt gcacggcttg
     8401 cacggctcgg tcgcgctcac cacgtcggag aacgtcacgc tgtccaggca gggctcacac
     8461 acggtctggt tggctccaca aggctgggcc acaccctcgc ccaggttgca ggctttgcag
     8521 cactcaccgc tgtgtgtgta caggcctgtg gggcatgcct ccttggcacc tccaagggac
     8581 acccccagaa gcagcaacag cagcaggcgc ggcccgtcca tggcgcggcc ggtggcacct
     8641 gcccccatcg cccgcctccc gcggcagcgc tcgacttcca gctcggtccg ctttgcggac
     8701 tgatggggct gcgctgcgct gcgctccagc gccccccctg cccgccggag ctggccgcgg
     8761 cccgaattcc tgcagcccgg gggatccccc tggggagaga ggtcggtgat tcggtcaacg
     8821 agggagccga ctgccgacgt gcgctccgga ggcttgcaga atgcggaaca ccgcgcgggc
     8881 aggaacaggg cccacactac cgccccacac cccgcctccc gcaccgcccc ttcccggccg
     8941 ctgctctcgg cgcgccctgc tgagcagccg ctattggcca cagcccatcg cggtcggcgc
     9001 gctgccattg ctccctggcg ctgtccgtct gcgagggtac tagtgagacg tgcggcttcc
     9061 gtttgtcacg tccggcacgc cgcgaaccgc aaggaacctt cccgacttag gggcggagca
     9121 ggaagcgtcg ccggggggcc cacaagggta gcggcgaaga tccgggtgac gctgcgaacg
     9181 gacgtgaaga atgtgcgaga cccagggtcg gcgccgctgc gtttcccgga accacgccca
     9241 gagcagccgc gtccctgcgc aaacccaggg ctgccttgga aaaggcgcaa ccccaacccc
     9301 gtggaattcg atatcaagct tctcgagggt aggcgtgtac ggtgggaggc ctatataagc
     9361 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
     9421 catagaagac accgggaccg atccagcctc cgcggaattc acc
//
