LOCUS       sequence-169983-        1431 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses PchA WT, the isochorismate synthase from Pseudomonas 
            aeruginosa.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Meneely KM, Luo Q, Dhar P, Lamb AL
  TITLE     Lysine221 is the general base residue of the isochorismate synthase 
            from Pseudomonas aeruginosa (PchA) in a reaction that is diffusion 
            limited.
  JOURNAL   Arch Biochem Biophys. 2013 Oct 1;538(1):49-56. doi: 
            10.1016/j.abb.2013.07.026. Epub 2013 Aug 11.
  PUBMED    23942051
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.abb.2013.07.026"; journalName: "Arch Biochem Biophys"; 
            date: "2013-10-1- 1"; volume: "538"; issue: "1"; pages: "49-56"
COMMENT     Sequence Label: pET28b-pchA WT
FEATURES             Location/Qualifiers
     source          1..1431
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgagccggc tggcgcccct gagccagtgc ctgcacgcct tgcgcggcac cttcgagcgc
       61 gccatcggcc aggcgcaggc gctcgatcgt ccggtgctgg tggcggcatc gttcgagatc
      121 gacccattgg acccgctgca ggtattcggt gcctgggacg accggcaaac gccctgcctg
      181 tactgggaac agcccgagct ggcgttcttc gcctggggct gcgccctgga gctgcaaggc
      241 cacggcgaac agcgcttcgc ccggatcgag gaaaactggc aattgctctg cgccgacgcc
      301 gtggtcgagg gcccgctggc gccgcgcctg tgcggcggat tccgcttcga tccgcgcggc
      361 ccgcgcgagg aacactggca agccttcgcc gatgccagcc tgatgctcgc cggcatcacc
      421 gtgctgcgcg agggcgaacg ctaccgggta ctctgccaac acctggccaa gcccggcgaa
      481 gatgccctgg ccctggccgc ctaccactgc tcggcgctac tgcgcctgag gcagccggcc
      541 agacgccggc cctcggggcc gaccgctggc gcgcagggcg acgcttcggc gcaggagcgc
      601 aggcaatggg aagccaaggt gagcgacgcg gtaagcagtg tccgccaggg acgcttcggc
      661 aaggtcgtgc tggcccgcac ccaggcccgg cctctcggcg acatcgagcc gtggcaggtc
      721 atcgaacacc tgcgtctgca acatgccgac gcccagctgt tcgcctgtcg ccgcggcaac
      781 gcctgcttcc tcggcgcctc cccggaacgc ctggtccgca ttcgcgccgg cgaggcactc
      841 acccatgccc tggccgggac catcgcccgc ggcggcgatg cccaggaaga tgcgcggctc
      901 ggacaggccc tgctggacag cgccaaggac aggcacgaac accagttggt ggtggaggcg
      961 atccgtacgg ccctggaacc cttcagcgag gtgctggaaa tccccgatgc gcccggcctg
     1021 aaacgactgg cgcgagtcca gcacctgaac acgccgatcc gcgcccgcct cgctgacgca
     1081 ggcggcatcc tgcggctgct acaagcgctg catccgaccc ccgcggtggg cggctaccca
     1141 cgcagcgcgg cgctggacta catccgccag cacgaaggga tggaccgcgg ctggtacgcc
     1201 gcgccgctgg gctggctcga cggcgaaggc aacggcgatt tcctggtggc gctgcgctcg
     1261 gccctgctca cgccgggccg gggctacctg ttcgccggct gcggtctggt aggcgattcg
     1321 gaaccggccc acgagtatcg cgaaacctgc cttaagctca gtgccatgcg ggaagctcta
     1381 tccgccatag gcggcctgga cgaagtgccc ttgcagcgcg gcgtcgcctg a
//
