LOCUS       sequence-170003-        9464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9464)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 9464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: 
            AB.pCCL.sin.cPPT.GFP.miR-19a-5p.sensor.PGK.dNGFR.WPRE
FEATURES             Location/Qualifiers
     source          1..9464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..3,4..6,7..720)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    997..1131
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    1268..1385
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1652..1696)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1881..2114
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2607..2732
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2779..2959
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2974..3177)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        3178..3557
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(3658..3678)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3658..3676)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3697..3713)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3697..3713)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3710..3732)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3721..3737
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3745..3751,3752..3769,3770..3775))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3790..3811
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3928..3945)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4099..4687)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4179..4198)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4858..5649,5650..5718))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5481..5500
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5719..5823)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5849..6304)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5986..6007)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6198..6217
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6431..6453
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6445..6462
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6446..6462
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6472..6491
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6472..6490
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      6511..6646
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6686..6807
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             6879..7112
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    7199..7787
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7305..7316
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7714..7734
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     promoter        complement(8791..9301)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8854..8872)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        9329..9367
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
      721 agcggccgcg tcgagagatg ggggaggcta actgagctag ccgaccttaa ctggggagta
      781 ctggtcgacg tagctgtagt gcaactatgc aaaacttgta gtgcaactat gcaaaacttg
      841 tagtgcaact atgcaaaact tgtagtgcaa ctatgcaaaa cttgtagtgc aactatgcaa
      901 aacttgtagt gcaactatgc aaaactaccg gtactagctt agtcatgcac cgggctgcag
      961 gaattcgatg acaggcgcag cttccgaggg atttgagatc cagacatgat aagatacatt
     1021 gatgagtttg gacaaaccaa aactagaatg cagtgaaaaa aatgccttat ttgtgaaatt
     1081 tgtgatgcta ttgccttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     1141 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     1201 taaaacctct acaaatgtgg tatggctgat tatgatcaga tctctcgagg ctagtctcgt
     1261 gatcgataaa attttgaatt tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg
     1321 ttgctattat gtctactatt ctttcccctg cactgtaccc cccaatcccc ccttttcttt
     1381 taaaagttaa ccgataccgt cgagatccgt tcactaatcg aatggatctg tctctgtctc
     1441 tctctccacc ttcttcttct attccttcgg gcctgtcggg tcccctcggg gttgggaggt
     1501 gggtctgaaa cgataatggt gaatatccct gcctaactct attcactata gaaagtacag
     1561 caaaaactat tcttaaacct accaagcctc ctactatcat tatgaataat tttatatacc
     1621 acagccaatt tgttatgtta aaccaattcc acaaacttgc ccatttatct aattccaata
     1681 attcttgttc attcttttct tgctggtttt gcgattcttc aattaaggag tgtattaagc
     1741 ttgtgtaatt gttaatttct ctgtcccact ccatccaggt cgtgtgattc caaatctgtt
     1801 ccagagattt attactccaa ctagcattcc aaggcacagc agtggtgcaa atgagttttc
     1861 cagagcaacc ccaaatcccc aggagctgtt gatcctttag gtatctttcc acagccagga
     1921 ttcttgcctg gagctgcttg atgccccaga ctgtgagttg caacagatgc tgttgcgcct
     1981 caatagccct cagcaaattg ttctgctgct gcactatacc agacaataat tgtctggcct
     2041 gtaccgtcag cgtcattgac gctgcgccca tagtgcttcc tgctgctccc aagaacccaa
     2101 ggaacaaagc tcctattccc actgctcttt tttctctctg caccactctt ctctttgcct
     2161 tggtgggtgc tactcctaat ggttcaattt ttactacttt atatttatat aattcacttc
     2221 tccaattgtc cctcatatct cctcctccag gtctgaagat cagcggccgc ttgctgtgcg
     2281 gtggtcttac ttttgttttg ctcttcctct atcttgtcta aagcttcctt ggtgtctttt
     2341 atctctatcc tttgatgcac acaatagagg gttgctactg tattatataa tgatctaagt
     2401 tcttctgatc ctgtctgaag ggatggttgt agctgtccca gtatttgtct acagccttct
     2461 gatgtttcta acaggccagg attaactgcg aatcgttcta gctccctgct tgcccatact
     2521 atatgtttta atttatattt tttctttccc cctggcctta accgaatttt ttcccatcgc
     2581 gatctaattc tcccccgctt aatactgacg ctctcgcacc catctctctc cttctagcct
     2641 ccgctagtca aaatttttgg cgtactcacc agtcgccgcc cctcgcctct tgccgtgcgc
     2701 gcttcagcaa gccgagtcct gcgtcgagag agctcctctg gtttcccttt cgctttcaag
     2761 tccctgttcg ggcgccactg ctagagattt tccacactga ctaaaagggt ctgagggatc
     2821 tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc
     2881 tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct
     2941 ggtctaacca gagagacccc ggttcactaa acgagctctg cttatataga cctcccaccg
     3001 tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc
     3061 ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa
     3121 tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc gcatcaccat
     3181 ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat
     3241 gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact
     3301 tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca
     3361 ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt
     3421 caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac
     3481 gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact attaataact
     3541 agtcaataat caatgtcaac atggcggtaa tgttggacat gagccaatat aaatgtacat
     3601 attatgatat ggatacaacg tatgcaatgg ccaagcttgc agctccagct tttgttccct
     3661 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     3721 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     3781 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     3841 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     3901 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     3961 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     4021 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     4081 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     4141 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4201 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4261 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     4321 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4381 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4441 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     4501 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     4561 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4621 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4681 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4741 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     4801 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     4861 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     4921 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     4981 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     5041 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     5101 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     5161 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     5221 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     5281 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     5341 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     5401 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     5461 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     5521 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     5581 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     5641 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     5701 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     5761 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     5821 gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc
     5881 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     5941 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     6001 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     6061 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     6121 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     6181 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     6241 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     6301 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     6361 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     6421 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac
     6481 tcactatagg gcgaattggg tacgtcccta ggcctccaaa aaagcctcct cactacttct
     6541 ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc
     6601 catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg
     6661 gcgggatagc tagagccaga catgataaga tacattgatg agtttggaca aaccacaact
     6721 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     6781 accattataa gctgcaataa acaagttcct ctcactctct gatattcatt tctttgcaag
     6841 ttataaatac tgaataataa gatgacatga actactactg ctagagattt tccacactga
     6901 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
     6961 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
     7021 gagctcccag gctcagatct ggtctaacca gagagaccca gtacaagcaa aaagcagatc
     7081 ttgtcttcgt tgggagtgaa ttagcccttc cagtcccccc ttttctttta aaaagtggct
     7141 aagatctaca gctgccttgt aagtcattgg tcttaaaggt accgagctcg aattccaggc
     7201 ggggaggcgg cccaaaggga gatccgactc gtctgagggc gaaggcgaag acgcggaaga
     7261 ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg
     7321 acgtagcaga aggacgtccc gcgcagaatc caggtggcaa cacaggcgag cagccatgga
     7381 aaggacgtca gcttccccga caacaccacg gaattgtcag tgcccaacag ccgagcccct
     7441 gtccagcagc gggcaaggca ggcggcgatg agttccgccg tggcaatagg gagggggaaa
     7501 gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg
     7561 tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa cgggccacaa ctcctcataa
     7621 agagacagca accaggattt atacaaggag gagaaaatga aagccatacg ggaagcaata
     7681 gcatgataca aaggcattaa agcagcgtat ccacatagcg taaaaggagc aacatagtta
     7741 agaataccag tcaatctttc acaaattttg taatccagag gttgattgtc gactctagac
     7801 tcgactctag aggatccccc tgttccacct cttgaaggct atgtaggcca caaggcccac
     7861 aaccacagca gccaggatgg agcaatagac agggatgagg ttgtcggtgg tgcctcgggt
     7921 caccacgggc tgggagctgc ccatcactgt ggtcaccaca cctgccaccg tgctggctat
     7981 gaggtcttgt tctggaggtg cctcaggctc ctgggtgctg ggggctgtgc tgtccgagcc
     8041 ctctgggggt gtggaccgtg taatccaacg gccagggatc tcctcgcact cggcgtcggc
     8101 ccagcgtgtg cactcgcgga gctggcgctc ggtgtcctcg cacacggtgc agggcaggca
     8161 cgggtccacg tggttggcct cgtcggaata cgtgccgtcg gggcactcct cgcacacggt
     8221 gttctgcttg tcctggcagg agaacacgag gcccgagccc gcctcgcaca cgcggcacgc
     8281 ctcgcagcgc ccagtcgtct catcctggta gtagccgtag gcgcagcggc acacggcgtc
     8341 gtcggcctcc acgcacggcg ccgacatgct ctggagcccc acgcactcgg tgcacggctt
     8401 gcacggctcg gtcgcgctca ccacgtcgga gaacgtcacg ctgtccaggc agggctcaca
     8461 cacggtctgg ttggctccac aaggctgggc cacaccctcg cccaggttgc aggctttgca
     8521 gcactcaccg ctgtgtgtgt acaggcctgt ggggcatgcc tccttggcac ctccaaggga
     8581 cacccccaga agcagcaaca gcagcaggcg cggcccgtcc atggcgcggc cggtggcacc
     8641 tgcccccatc gcccgcctcc cgcggcagcg ctcgacttcc agctcggtcc gctttgcgga
     8701 ctgatggggc tgcgctgcgc tgcgctccag cgccccccct gcccgccgga gctggccgcg
     8761 gcccgaattc ctgcagcccg ggggatcccc ctggggagag aggtcggtga ttcggtcaac
     8821 gagggagccg actgccgacg tgcgctccgg aggcttgcag aatgcggaac accgcgcggg
     8881 caggaacagg gcccacacta ccgccccaca ccccgcctcc cgcaccgccc cttcccggcc
     8941 gctgctctcg gcgcgccctg ctgagcagcc gctattggcc acagcccatc gcggtcggcg
     9001 cgctgccatt gctccctggc gctgtccgtc tgcgagggta ctagtgagac gtgcggcttc
     9061 cgtttgtcac gtccggcacg ccgcgaaccg caaggaacct tcccgactta ggggcggagc
     9121 aggaagcgtc gccggggggc ccacaagggt agcggcgaag atccgggtga cgctgcgaac
     9181 ggacgtgaag aatgtgcgag acccagggtc ggcgccgctg cgtttcccgg aaccacgccc
     9241 agagcagccg cgtccctgcg caaacccagg gctgccttgg aaaaggcgca accccaaccc
     9301 cgtggaattc gatatcaagc ttctcgaggg taggcgtgta cggtgggagg cctatataag
     9361 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     9421 ccatagaaga caccgggacc gatccagcct ccgcggaatt cacc
//
