LOCUS       sequence-170039-        9446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9446)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 9446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: AB.pCCL.sin.cPPT.GFP.miR-564.sensor.PGK.dNGFR....
FEATURES             Location/Qualifiers
     source          1..9446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..3,4..6,7..720)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    979..1113
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    1250..1367
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1634..1678)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1863..2096
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2589..2714
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2761..2941
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2956..3159)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        3160..3539
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(3640..3660)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3640..3658)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3679..3695)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3679..3695)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3692..3714)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3703..3719
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3727..3733,3734..3751,3752..3757))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3772..3793
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3910..3927)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4081..4669)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4161..4180)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4840..5631,5632..5700))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5463..5482
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5701..5805)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5831..6286)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5968..5989)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6180..6199
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6413..6435
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6427..6444
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6428..6444
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6454..6473
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6454..6472
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      6493..6628
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6668..6789
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             6861..7094
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    7181..7769
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7287..7298
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7696..7716
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     promoter        complement(8773..9283)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8836..8854)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        9311..9349
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
      721 agcggccgcg tcgagagatg ggggaggcta actgagctag ccgaccttaa ctggggagta
      781 ccaatcgtcg tagcgcctgc tgacaccgtg cctgcctgct gacaccgtgc ctgcctgctg
      841 acaccgtgcc tgcctgctga caccgtgcct gcctgctgac accgtgcctg cctgctgaca
      901 ccgtgcctac cggtactagc ttagtcatgc accgggctgc aggaattcga tgacaggcgc
      961 agcttccgag ggatttgaga tccagacatg ataagataca ttgatgagtt tggacaaacc
     1021 aaaactagaa tgcagtgaaa aaaatgcctt atttgtgaaa tttgtgatgc tattgcctta
     1081 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     1141 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     1201 ggtatggctg attatgatca gatctctcga ggctagtctc gtgatcgata aaattttgaa
     1261 tttttgtaat ttgtttttgt aattctttag tttgtatgtc tgttgctatt atgtctacta
     1321 ttctttcccc tgcactgtac cccccaatcc ccccttttct tttaaaagtt aaccgatacc
     1381 gtcgagatcc gttcactaat cgaatggatc tgtctctgtc tctctctcca ccttcttctt
     1441 ctattccttc gggcctgtcg ggtcccctcg gggttgggag gtgggtctga aacgataatg
     1501 gtgaatatcc ctgcctaact ctattcacta tagaaagtac agcaaaaact attcttaaac
     1561 ctaccaagcc tcctactatc attatgaata attttatata ccacagccaa tttgttatgt
     1621 taaaccaatt ccacaaactt gcccatttat ctaattccaa taattcttgt tcattctttt
     1681 cttgctggtt ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt
     1741 ctctgtccca ctccatccag gtcgtgtgat tccaaatctg ttccagagat ttattactcc
     1801 aactagcatt ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc
     1861 ccaggagctg ttgatccttt aggtatcttt ccacagccag gattcttgcc tggagctgct
     1921 tgatgcccca gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat
     1981 tgttctgctg ctgcactata ccagacaata attgtctggc ctgtaccgtc agcgtcattg
     2041 acgctgcgcc catagtgctt cctgctgctc ccaagaaccc aaggaacaaa gctcctattc
     2101 ccactgctct tttttctctc tgcaccactc ttctctttgc cttggtgggt gctactccta
     2161 atggttcaat ttttactact ttatatttat ataattcact tctccaattg tccctcatat
     2221 ctcctcctcc aggtctgaag atcagcggcc gcttgctgtg cggtggtctt acttttgttt
     2281 tgctcttcct ctatcttgtc taaagcttcc ttggtgtctt ttatctctat cctttgatgc
     2341 acacaataga gggttgctac tgtattatat aatgatctaa gttcttctga tcctgtctga
     2401 agggatggtt gtagctgtcc cagtatttgt ctacagcctt ctgatgtttc taacaggcca
     2461 ggattaactg cgaatcgttc tagctccctg cttgcccata ctatatgttt taatttatat
     2521 tttttctttc cccctggcct taaccgaatt ttttcccatc gcgatctaat tctcccccgc
     2581 ttaatactga cgctctcgca cccatctctc tccttctagc ctccgctagt caaaattttt
     2641 ggcgtactca ccagtcgccg cccctcgcct cttgccgtgc gcgcttcagc aagccgagtc
     2701 ctgcgtcgag agagctcctc tggtttccct ttcgctttca agtccctgtt cgggcgccac
     2761 tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac
     2821 acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca
     2881 gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc
     2941 ccggttcact aaacgagctc tgcttatata gacctcccac cgtacacgcc taccgcccat
     3001 ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca
     3061 aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg
     3121 ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa
     3181 tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca
     3241 ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg
     3301 atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt
     3361 ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt
     3421 gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg
     3481 gctatgaact aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtca
     3541 acatggcggt aatgttggac atgagccaat ataaatgtac atattatgat atggatacaa
     3601 cgtatgcaat ggccaagctt gcagctccag cttttgttcc ctttagtgag ggttaattgc
     3661 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     3721 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     3781 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     3841 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     3901 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3961 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4021 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4081 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4141 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4201 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4261 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4321 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4381 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4441 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4501 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     4561 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4621 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4681 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4741 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     4801 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     4861 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     4921 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     4981 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     5041 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     5101 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     5161 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     5221 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     5281 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     5341 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     5401 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     5461 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     5521 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     5581 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     5641 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     5701 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     5761 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     5821 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     5881 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     5941 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     6001 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     6061 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     6121 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     6181 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     6241 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
     6301 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     6361 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     6421 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
     6481 ggtacgtccc taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg
     6541 aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg
     6601 cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggata gctagagcca
     6661 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     6721 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     6781 aaacaagttc ctctcactct ctgatattca tttctttgca agttataaat actgaataat
     6841 aagatgacat gaactactac tgctagagat tttccacact gactaaaagg gtctgaggga
     6901 tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa
     6961 gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat
     7021 ctggtctaac cagagagacc cagtacaagc aaaaagcaga tcttgtcttc gttgggagtg
     7081 aattagccct tccagtcccc ccttttcttt taaaaagtgg ctaagatcta cagctgcctt
     7141 gtaagtcatt ggtcttaaag gtaccgagct cgaattccag gcggggaggc ggcccaaagg
     7201 gagatccgac tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc
     7261 aggccgcggg aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc
     7321 ccgcgcagaa tccaggtggc aacacaggcg agcagccatg gaaaggacgt cagcttcccc
     7381 gacaacacca cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg
     7441 caggcggcga tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg
     7501 agctgacagg tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac
     7561 accacgccac gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat
     7621 ttatacaagg aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt
     7681 aaagcagcgt atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt
     7741 tcacaaattt tgtaatccag aggttgattg tcgactctag actcgactct agaggatccc
     7801 cctgttccac ctcttgaagg ctatgtaggc cacaaggccc acaaccacag cagccaggat
     7861 ggagcaatag acagggatga ggttgtcggt ggtgcctcgg gtcaccacgg gctgggagct
     7921 gcccatcact gtggtcacca cacctgccac cgtgctggct atgaggtctt gttctggagg
     7981 tgcctcaggc tcctgggtgc tgggggctgt gctgtccgag ccctctgggg gtgtggaccg
     8041 tgtaatccaa cggccaggga tctcctcgca ctcggcgtcg gcccagcgtg tgcactcgcg
     8101 gagctggcgc tcggtgtcct cgcacacggt gcagggcagg cacgggtcca cgtggttggc
     8161 ctcgtcggaa tacgtgccgt cggggcactc ctcgcacacg gtgttctgct tgtcctggca
     8221 ggagaacacg aggcccgagc ccgcctcgca cacgcggcac gcctcgcagc gcccagtcgt
     8281 ctcatcctgg tagtagccgt aggcgcagcg gcacacggcg tcgtcggcct ccacgcacgg
     8341 cgccgacatg ctctggagcc ccacgcactc ggtgcacggc ttgcacggct cggtcgcgct
     8401 caccacgtcg gagaacgtca cgctgtccag gcagggctca cacacggtct ggttggctcc
     8461 acaaggctgg gccacaccct cgcccaggtt gcaggctttg cagcactcac cgctgtgtgt
     8521 gtacaggcct gtggggcatg cctccttggc acctccaagg gacaccccca gaagcagcaa
     8581 cagcagcagg cgcggcccgt ccatggcgcg gccggtggca cctgccccca tcgcccgcct
     8641 cccgcggcag cgctcgactt ccagctcggt ccgctttgcg gactgatggg gctgcgctgc
     8701 gctgcgctcc agcgcccccc ctgcccgccg gagctggccg cggcccgaat tcctgcagcc
     8761 cgggggatcc ccctggggag agaggtcggt gattcggtca acgagggagc cgactgccga
     8821 cgtgcgctcc ggaggcttgc agaatgcgga acaccgcgcg ggcaggaaca gggcccacac
     8881 taccgcccca caccccgcct cccgcaccgc cccttcccgg ccgctgctct cggcgcgccc
     8941 tgctgagcag ccgctattgg ccacagccca tcgcggtcgg cgcgctgcca ttgctccctg
     9001 gcgctgtccg tctgcgaggg tactagtgag acgtgcggct tccgtttgtc acgtccggca
     9061 cgccgcgaac cgcaaggaac cttcccgact taggggcgga gcaggaagcg tcgccggggg
     9121 gcccacaagg gtagcggcga agatccgggt gacgctgcga acggacgtga agaatgtgcg
     9181 agacccaggg tcggcgccgc tgcgtttccc ggaaccacgc ccagagcagc cgcgtccctg
     9241 cgcaaaccca gggctgcctt ggaaaaggcg caaccccaac cccgtggaat tcgatatcaa
     9301 gcttctcgag ggtaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa
     9361 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
     9421 ccgatccagc ctccgcggaa ttcacc
//
