LOCUS       sequence-170058-       11741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This vector is used to specifically silence the eIF(iso)4E gene in 
            peach via VIGS..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11741)
  AUTHORS   Cui H, Wang A
  TITLE     An efficient viral vector for functional genomic studies of Prunus 
            fruit trees and its induced resistance to Plum pox virus via 
            silencing of a host factor gene.
  JOURNAL   Plant Biotechnol J. 2016 Aug 27. doi: 10.1111/pbi.12629.
  PUBMED    27565765
REFERENCE   2  (bases 1 to 11741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2016 Aug 27. doi: 10.1111/pbi.12629."
COMMENT     Sequence Label: pCaRNA3-eIFiso4E120(+)
FEATURES             Location/Qualifiers
     source          1..11741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    602..623
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(638..643,644..661,662..668)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    676..692
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     681..703
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     700..716
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     700..716
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1125..1170
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_RNA        3259..3310
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     polyA_signal    3383..3559
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(3568..3585)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3568..3584)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3577..3599)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3787..3811
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5112..5741
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6170..7243
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7309..7503
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7739..7761)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7847..7987
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7902..7921
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8002..8019)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8173..8761)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8253..8272)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8848..9642)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    10067..10091
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    10169..10343
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(10384..11409)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(join(11476..11741,1..411))
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg
       61 atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact gtatctttga
      121 tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt cacatcaatc cacttgcttt
      181 gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct
      241 ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaatgatgg
      301 catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg
      361 caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt
      421 tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc
      481 atgttggcaa gctgctctag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
      541 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
      601 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
      661 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga
      721 ttacgaattc tgaagcttgc atgcctgcag gtcaacatgg tggagcacga cactctcgtc
      781 tactccaaga atatcaaaga tacagtctca gaagaccaga gggctattga gacttttcaa
      841 caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc
      901 gaaaggacag tagaaaagga agatggcttc tacaaatgcc atcattgcga taaaggaaag
      961 gctatcgttc aagatgcctc taccgacagt ggtcccaaag atggaccccc acccacgagg
     1021 aacatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat
     1081 atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct
     1141 atataaggaa gttcatttca tttggagagg gtttttacaa attgaaatcg taacatccag
     1201 taattggttg atttctcttt tacttgacta ggtaccattg aaacgaatta aaagtcctat
     1261 ttctctagta acatcccctt tgtgggttta gagattgttg gttgtctatt cgtaattcca
     1321 cttgttggtt gaatagtgtt tcagtatggc cggtgtcagt aaaaacccct ccactagtga
     1381 tttttcagta gtggagtgtt ctatggacga aatgagccag atatcagagg atttgcacaa
     1441 acttatgctg tccgatgaga tgaaggcgtt accaacgaaa ggttgtcata tcctgcattt
     1501 ggttaacctc ccgaaatcca atatattgag attagcgagt aaggagcaga aaggtttcct
     1561 ctcgcgacag gccgataaag taaagaagaa aatttacaga tgtgtaggcc gagtattcct
     1621 cgtttatgta ccgattattc aggcaacgac ctcgggtctg ataaccctta agttgcaaaa
     1681 ctccgataca ggagagattt cagacgtcgt gacagacgtc gaagccaacc gcgcctttgt
     1741 tattatggac agatggggaa gatccctggt ggaatcagca gatttaaatc tgttgtattc
     1801 catttcatgt ccggacgtcc gacctggagc cagagtggga gagatgatgg ctttttggga
     1861 cgagaggatg tcaaggcagc agacttactt ggagaaaggg aatcctattt tattcccgat
     1921 tgccgagaca aaaccttcga aatatcttaa tgataagaag gtgttgatgt ctatggtccg
     1981 aagtaggata ttggcaggta cagaagggtg cgatatagct cccgagaata tagaagtcaa
     2041 acgattaggg gatagtagga aggttttgac cattcaaccg aaagccccga ttgtagagga
     2101 aattaaggac gatgttgaac cgttaggttc gaatggtgaa aatcatatgg aagagaagac
     2161 cgtgaccgtt aaggtcggta gttctggaag tgcttgagtg actatgtcac gagcgtgcgc
     2221 tttgcgcgag cgtttttctt tcttttcttc cgaacatctt tttcatttga taatggtttg
     2281 ccgaatttgc aatcataccc acgctggtgg atgccgttct tgcaagaagt gccatccgaa
     2341 tgatgctctg gtcccactca gggctcaaca aagggctgcg aataacccga ataggaatag
     2401 gaacccgaat agggtttcga gcggtatagg acctgcggtc cgaccgcaac cggtcgtgaa
     2461 gaccacttgg accgtgaggg gtccgaatgt gcctccccga attcccaagg gttatgtggc
     2521 acataatcac cgagaggtga cgacgacaga ggcagtgaag tacttgagca ttgacttcac
     2581 gaccactctc cctcagttga tgggtcagaa tttgacctta ttaaccgtta tagtccgaat
     2641 gaactctatg agttcgaatg gttggattgg gatggtggag gactataagg tggatcaacc
     2701 tgatggtccg aatgccctgt ctaggaaggg gttcttaaag gaccaaccga gaggttggca
     2761 gttcgaacct ccctccgatt tagatttcga cacttttgcg cgtacgcatc gtgtcgtcat
     2821 cgaattcaag accgaagtgc ccgctggggc caaggtcttg gttagggatt tgtacgtagt
     2881 ggtaagtgac ttaccacgag tgcaaattcc gaccgatgtc ttgctggtcg atgaagacct
     2941 gcttgagatc tagaatggcg acagaggtag cagcagcagc agttccgcca ccacaattag
     3001 acgctgagga aaatagtggg ttagaggcgg cggcggccga ggcaaagata cagccgagca
     3061 gtgggcccca caagtctaga gtgagataag cacactcgaa tgtctccgaa tggaaagttc
     3121 gtaccaccga tagtggatat tgcgaaatag atttctgaaa gtcgcttccc ggctttcatg
     3181 cttggaaatc ttacctgcgt tagcagatgc ccacaacgtg aagttgtgga tgccccgtta
     3241 gggaagcgga tccgataccc tgtcaccgga tgtgttttcc ggtctgatga gtccgtgagg
     3301 acgaaacagg actgtcctgc aggagctcga attcggtacg ctgaaatcac cagtctctct
     3361 ctacaaatct atctctctct attttctcca taaataatgt gtgagtagtt tcccgataag
     3421 ggaaattagg gttcttatag ggtttcgctc atgtgttgag catataagaa acccttagta
     3481 tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc
     3541 cagtactaaa atccagatca gcttggcact ggccgtcgtt ttacaacgtc gtgactggga
     3601 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     3661 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     3721 atgctagagc agcttgagct tggatcagat tgtcgtttcc cgccttcagt ttaaactatc
     3781 agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa
     3841 tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca
     3901 accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct gctatagtgc
     3961 agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta
     4021 agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag
     4081 tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc
     4141 cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca
     4201 acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac
     4261 caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt
     4321 gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga
     4381 gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac
     4441 cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc
     4501 cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt
     4561 tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca
     4621 ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta
     4681 ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt
     4741 ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga
     4801 ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag
     4861 atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc
     4921 gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc
     4981 agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa
     5041 aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc
     5101 aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga
     5161 ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg
     5221 attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa
     5281 ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg
     5341 acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg
     5401 cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg
     5461 acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct
     5521 ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg
     5581 ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca
     5641 ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg
     5701 tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa
     5761 aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc
     5821 tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc
     5881 ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga
     5941 gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta
     6001 gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg
     6061 ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg
     6121 cctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg
     6181 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt
     6241 gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc
     6301 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc
     6361 gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct
     6421 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc
     6481 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga
     6541 ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc
     6601 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg
     6661 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg
     6721 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat
     6781 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt
     6841 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga
     6901 gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt
     6961 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg
     7021 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg
     7081 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga
     7141 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg
     7201 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct
     7261 agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac
     7321 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa
     7381 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga
     7441 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc
     7501 ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct
     7561 gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat
     7621 ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga
     7681 ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     7741 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     7801 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     7861 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
     7921 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     7981 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8041 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8101 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8161 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8221 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8281 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8341 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8401 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8461 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8521 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8581 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     8641 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8701 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8761 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8821 agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt
     8881 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc
     8941 ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc
     9001 cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt
     9061 gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa
     9121 aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc
     9181 cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct
     9241 attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata
     9301 cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc
     9361 atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt
     9421 tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata
     9481 ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac
     9541 cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc
     9601 gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct
     9661 tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca
     9721 ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca
     9781 aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca
     9841 gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca
     9901 aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc
     9961 gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg
    10021 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
    10081 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
    10141 actgaattaa cgccgaatta attcggggga tctggatttt agtactggat tttggtttta
    10201 ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac taagggtttc
    10261 ttatatgctc aacacatgag cgaaacccta taggaaccct aattccctta tctgggaact
    10321 actcacacat tattatggag aaactcgagc ttgtcgatcg acagatcccg gtcggcatct
    10381 actctatttc tttgccctcg gacgagtgct ggggcgtcgg tttccactat cggcgagtac
    10441 ttctacacag ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg tacgcccgac
    10501 agtcccggct ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc
    10561 gaaattgccg tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga gcatatacgc
    10621 ccggagtcgt ggcgatcctg caagctccgg atgcctccgc tcgaagtagc gcgtctgctg
    10681 ctccatacaa gccaaccacg gcctccagaa gaagatgttg gcgacctcgt attgggaatc
    10741 cccgaacatc gcctcgctcc agtcaatgac cgctgttatg cggccattgt ccgtcaggac
    10801 attgttggag ccgaaatccg cgtgcacgag gtgccggact tcggggcagt cctcggccca
    10861 aagcatcagc tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt
    10921 ttgccagtga tacacatggg gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta
    10981 ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa gatcggccgc
    11041 agcgatcgca tccatagcct ccgcgaccgg ttgtagaaca gcgggcagtt cggtttcagg
    11101 caggtcttgc aacgtgacac cctgtgaacg gcgggagatg caataggtca ggctctcgct
    11161 aaactcccca atgtcaagca cttccggaat cgggagcgcg gccgatgcaa agtgccgata
    11221 aacataacga tctttgtaga aaccatcggc gcagctattt acccgcagga catatccacg
    11281 ccctcctaca tcgaagctga aagcacgaga ttcttcgccc tccgagagct gcatcaggtc
    11341 ggagacgctg tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg
    11401 ctttttcata tctcattgcc ccccggatct gcgaaagctc gagagagata gatttgtaga
    11461 gagagactgg tgatttcagc gtgtcctctc caaatgaaat gaacttcctt atatagagga
    11521 agggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca
    11581 tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt
    11641 gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc
    11701 tttatcgcaa tgatggcatt tgtaggtgcc accttccttt t
//
