LOCUS       sequence-170062-         538 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express mouse PRMT7 with Flag-tag in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Lee SH, Chen TY, Dhar SS, Gu B, Chen K, Kim YZ, Li W, Lee MG
  TITLE     A feedback loop comprising PRMT7 and miR-24-2 interplays with Oct4, 
            Nanog, Klf4 and c-Myc to regulate stemness.
  JOURNAL   Nucleic Acids Res. 2016 Dec 15;44(22):10603-10618. Epub 2016 Sep 12.
  PUBMED    27625395
REFERENCE   2  (bases 1 to 538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2016-12-15"; volume: "44(22)"; pages: 
            "10603-10618. Epub 2016 Sep 12"
COMMENT     Sequence Label: pCDH-Flag-mPRMT7-GFP
FEATURES             Location/Qualifiers
     source          1..538
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..51
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 accggcgcct actctagagc caccatggac tacaaagacg atgacgacaa gaaggtcttc
       61 tgtggccggg ccaatcctac cacgggatcc ctggagtggc tggaggagga tgaacactat
      121 gattaccacc aggagattgc caggtcatcc tatgccgaca tgctacatga caaagacaga
      181 aatataaaat actaccaggg tatccgggca gctgtgagca gggtgaaaga cagaggacag
      241 aaggccttgg ttcttgacat tggcactggc acaggcctct tgtcaatgat ggcagttact
      301 gcaggggctg acttctgcta tgctatcgag gtttttaagc ctatggctga ggctgctgtg
      361 aagattgtgg agaggaatgg ctttcagtga taagattaaa gtcattaaca agcactccac
      421 tgaggtgaca gtcggaccag atggtgactt gccgtgtcgt gctaacattc tgatcacgga
      481 gctgtttgac acagagctga ttggggaggg agcgctgccc tcttatgagc atgcacac
//
