LOCUS       sequence-170067-        4570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression in mycobacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Beckham KSH, Staack S, Wilmanns M, Parret AHA
  TITLE     The pMy vector series: A versatile cloning platform for the 
            recombinant production of mycobacterial proteins in Mycobacterium 
            smegmatis.
  JOURNAL   Protein Sci. 2020 Oct 2. doi: 10.1002/pro.3962.
  PUBMED    33006405
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Oct 2. doi: 10.1002/pro.3962."
COMMENT     Sequence Label: pMyBADNT-kan
FEATURES             Location/Qualifiers
     source          1..4570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(19..65)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(162..182)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(195..212)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(249..533)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(307..326)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             560..1438
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     rep_origin      complement(3530..4118)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3610..3629)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 caccgcggcc atgatggcat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt
       61 atttgatgcc tggcagtcga tcgtacgcta gttaactacg tcgacatcga taagcttgct
      121 agcgatatcg aattcggatc cggtacctta tcgtccatgg cgccctggaa gtacaggttc
      181 tcgccggccg acgggtggtg gtggtggtgg tgcatggtta attcctcctg ttagcccaaa
      241 aaacgggtat ggagaaacag tagagagttg cgataaaaag cgtcaggtag gatccgctaa
      301 tcttatggat aaaaatgcta tggcatagca aagtgtgacg ccgtgcaaat aatcaatgtg
      361 gacttttctg ccgtgattat agacactttt gttacgcgtt tttgtcatgg ctttggtccc
      421 gctttgttac agaatgcttt taataagcgg ggttaccggt ttggttagcg agaagagcca
      481 gtaaaagacg cagtgacggc aatgtctgat gcaatatgga caattggttt cttctctgaa
      541 tggcgggagt atgaaaagta tggctgaagc gcaaaatgat cccctgctgc cgggatactc
      601 gtttaatgcc catctggtgg cgggtttaac gccgattgag gccaacggtt atctcgattt
      661 ttttatcgac cgaccgctgg gaatgaaagg ttatattctc aatctcacca ttcgcggtca
      721 gggggtggtg aaaaatcagg gacgagaatt tgtttgccga ccgggtgata ttttgctgtt
      781 cccgccagga gagattcatc actacggtcg tcatccggag gctcgcgaat ggtatcacca
      841 gtgggtttac tttcgtccgc gcgcctactg gcatgaatgg cttaactggc cgtcaatatt
      901 tgccaatacg gggttctttc gcccggatga agcgcaccag ccgcatttca gcgacctgtt
      961 tgggcaaatc attaacgccg ggcaagggga agggcgctat tcggagctgc tggcgataaa
     1021 tctgcttgag caattgttac tgcggcgcat ggaagcgatt aacgagtcgc tccatccacc
     1081 gatggataat cgggtacgcg aggcttgtca gtacatcagc gatcacctgg cagacagcaa
     1141 ttttgatatc gccagcgtcg cacagcatgt ttgcttgtcg ccgtcgcgtc tgtcacatct
     1201 tttccgccag cagttaggga ttagcgtctt aagctggcgc gaggaccaac gtatcagcca
     1261 ggcgaagctg cttttgagca ccacccggat gcctatcgcc accgtcggtc gcaatgttgg
     1321 ttttgacgat caactctatt tctcgcgggt atttaaaaaa tgcaccgggg ccagcccgag
     1381 cgagttccgt gccggttgtg aagaaaaagt gaatgatgta gccgtcaagt tgtcataatc
     1441 tagatttaaa gatctggtac cgcggccgcg ccacggatgc caccacaggc actacaacgg
     1501 agttcgccac gtacatcacc acaaccaccg attctggcgg tgagctcccc gatattcagc
     1561 ggaaatggct tggtatcgac caagattcgt agaaccccgt ctcgtctggc tggtattcaa
     1621 aacgggcgca acgaaacacg caacgagaca ggcatggccc aaaccagaaa actagcgtct
     1681 accaggactt ttacctgtcc gacccgttgc aacggaaccc cccacggaac ccccgcgaca
     1741 cccgctcccc aattgcgtta gaacagcggt ggattgtcgg cttcgttgtg ggccttttga
     1801 gccgcttcct gttctgccgc acgctctttc ctcgcccgat agccgagtcg cttaacggtg
     1861 tccagatgca gcccgaaatg tttggccgtt tgcggccaag agtggccctc gtcgtcgtga
     1921 taggcgcgga tgcgttcgcg gcgtgcagcc tgctcggcga gccactcgct gcgttcctgc
     1981 gccacgagcc ggacgacgtg gcgttcggat agtccggtga ttcgagcgcc ttcggcggcg
     2041 gtcacgcgcc gctttttgcg gacagtcggc tgccggttgt agccgtcgct gtagccgtcg
     2101 ctcatagcaa tgcctccatg catggctgac gcggactttg cgcgccgcgc aactgtgctc
     2161 gccgccgtgc gcgctgctgc gcccttccgc gagatggccg actggcgcgc actgagtgtg
     2221 gcctcgtaga ccacgatccc gtccgcccaa atgcgcgact tggttgtgat ccaacgccaa
     2281 atgctgttgg cgatggcgcg gacctcgctg tccggtagcg gtccgggaca cacgtcgttg
     2341 cacggaaatt cggcgtttcg cgcgtggcac tcggcataga tcgcgcggcc gagtccgtcc
     2401 acgttccggg tcggcaggta gatccgcatg agggcgggac gataggccca caacctgacg
     2461 gaatcgaaca gtgcgcaatt ccgccctagc ggcgtcggag ccgctttgta cgtggtctgc
     2521 tgacgccagc gcggcggtgg catgttcgcg ccgagctcgg cctcgatgtg gctgagtgtg
     2581 tagagatctg agtggagcca ttccgtttcc caggcgatgt ggccggggtt tttggtcatg
     2641 aggcctgagt aactgcggtc gccatcgacg gcgcgccgaa ggccttcggc gcacgccgcc
     2701 atgtatgcga gcggcttacg ccgcgcgtat tcggtgcgtg gaacaggggc gttgagtgcc
     2761 cacactgcgt gtgcgtggcc gttggcgcga ttgcccacga tcgcgttggg cagcggatgg
     2821 gacccccggg cgctgagcgc tcggagcgct gcgtctggat ggtctacgtc cacgaccagc
     2881 aggtttgcca gcgctgttgg gttcgcctcg atgtaccggc ggcctagggc cgacgcgcgg
     2941 ctttggcggt agatcccctc gagcagatcg tcgcttgcca gcggccagta cggcagccag
     3001 agctgctcaa attcgtcggc gacgtggctc acgcttggta gtagaccacg attaatcacc
     3061 ggtgtatggt ccgacacgag ctccaagtca gatatttcgc tgaggggcca ccccacaact
     3121 gcacactccc ccgctctccc gtcgagccct ggtggtggaa caccagcgac agccgagcac
     3181 ccccaaccac ctgtaccaac caggaggaac acatgcgtcg tttcgaggac gtttccgggc
     3241 cgctgagagc cgctgtggcg gccgtacacg ccgccttaga cccgttagac cccctgccgc
     3301 ctgaatgcgc gggtacgagc cacacagcgc ccgaacttac ggagctggtg ggctcacgcg
     3361 tgcggccgca cgcgttgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     3421 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     3481 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     3541 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     3601 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3661 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ctcgggaagc gtggcgcttt
     3721 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3781 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3841 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     3901 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     3961 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     4021 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     4081 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     4141 cggggtctga cgctcatgga actagagcat gagattatca aaaaggatct tcacctagat
     4201 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     4261 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     4321 atccatagtt gcctgactcc ccgacgtggc cgaccagccc gtcatcgtca acgcctgatc
     4381 cgcggtgcgg acaggccgtg tcgcgaccgg ccgtgcggaa ttaagccggc ccgtaccctg
     4441 tgaatagagg tccgctcaac aaagcgacgt tgtgtctcaa aatctctgat gttacattgc
     4501 acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac
     4561 aaggggtgtt
//
