LOCUS       sequence-170073-       11615 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of cDNA of full-length genomic RNA3 of PNRSV (Pch12 
            isolate) in transfected cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11615)
  AUTHORS   Cui H, Wang A
  TITLE     An efficient viral vector for functional genomic studies of Prunus 
            fruit trees and its induced resistance to Plum pox virus via 
            silencing of a host factor gene.
  JOURNAL   Plant Biotechnol J. 2016 Aug 27. doi: 10.1111/pbi.12629.
  PUBMED    27565765
REFERENCE   2  (bases 1 to 11615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2016 Aug 27. doi: 10.1111/pbi.12629."
COMMENT     Sequence Label: pCaRNA3
FEATURES             Location/Qualifiers
     source          1..11615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    343..364
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(379..384,385..402,403..409)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    417..433
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     422..444
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     441..457
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     441..457
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        866..911
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_RNA        2874..2925
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     polyA_signal    2998..3174
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(3183..3200)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3183..3199)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3192..3214)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3402..3426
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4727..5356
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5785..6858
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6924..7118
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7354..7376)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7462..7602
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7517..7536
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7617..7634)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7788..8376)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7868..7887)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8463..9257)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9682..9706
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    9784..9958
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9999..11024)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(join(11091..11615,1..152))
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt
       61 ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt
      121 ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat
      181 ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttggca agctgctcta
      241 gccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
      301 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
      361 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
      421 gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt ctgaagcttg
      481 catgcctgca ggtcaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag
      541 atacagtctc agaagaccag agggctattg agacttttca acaaagggta atatcgggaa
      601 acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca gtagaaaagg
      661 aagatggctt ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct
      721 ctaccgacag tggtcccaaa gatggacccc cacccacgag gaacatcgtg gaaaaagaag
      781 acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg
      841 atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc
      901 atttggagag ggtttttaca aattgaaatc gtaacatcca gtaattggtt gatttctctt
      961 ttacttgact aggtaccatt gaaacgaatt aaaagtccta tttctctagt aacatcccct
     1021 ttgtgggttt agagattgtt ggttgtctat tcgtaattcc acttgttggt tgaatagtgt
     1081 ttcagtatgg ccggtgtcag taaaaacccc tccactagtg atttttcagt agtggagtgt
     1141 tctatggacg aaatgagcca gatatcagag gatttgcaca aacttatgct gtccgatgag
     1201 atgaaggcgt taccaacgaa aggttgtcat atcctgcatt tggttaacct cccgaaatcc
     1261 aatatattga gattagcgag taaggagcag aaaggtttcc tctcgcgaca ggccgataaa
     1321 gtaaagaaga aaatttacag atgtgtaggc cgagtattcc tcgtttatgt accgattatt
     1381 caggcaacga cctcgggtct gataaccctt aagttgcaaa actccgatac aggagagatt
     1441 tcagacgtcg tgacagacgt cgaagccaac cgcgcctttg ttattatgga cagatgggga
     1501 agatccctgg tggaatcagc agatttaaat ctgttgtatt ccatttcatg tccggacgtc
     1561 cgacctggag ccagagtggg agagatgatg gctttttggg acgagaggat gtcaaggcag
     1621 cagacttact tggagaaagg gaatcctatt ttattcccga ttgccgagac aaaaccttcg
     1681 aaatatctta atgataagaa ggtgttgatg tctatggtcc gaagtaggat attggcaggt
     1741 acagaagggt gcgatatagc tcccgagaat atagaagtca aacgattagg ggatagtagg
     1801 aaggttttga ccattcaacc gaaagccccg attgtagagg aaattaagga cgatgttgaa
     1861 ccgttaggtt cgaatggtga aaatcatatg gaagagaaga ccgtgaccgt taaggtcggt
     1921 agttctggaa gtgcttgagt gactatgtca cgagcgtgcg ctttgcgcga gcgtttttct
     1981 ttcttttctt ccgaacatct ttttcatttg ataatggttt gccgaatttg caatcatacc
     2041 cacgctggtg gatgccgttc ttgcaagaag tgccatccga atgatgctct ggtcccactc
     2101 agggctcaac aaagggctgc gaataacccg aataggaata ggaacccgaa tagggtttcg
     2161 agcggtatag gacctgcggt ccgaccgcaa ccggtcgtga agaccacttg gaccgtgagg
     2221 ggtccgaatg tgcctccccg aattcccaag ggttatgtgg cacataatca ccgagaggtg
     2281 acgacgacag aggcagtgaa gtacttgagc attgacttca cgaccactct ccctcagttg
     2341 atgggtcaga atttgacctt attaaccgtt atagtccgaa tgaactctat gagttcgaat
     2401 ggttggattg ggatggtgga ggactataag gtggatcaac ctgatggtcc gaatgccctg
     2461 tctaggaagg ggttcttaaa ggaccaaccg agaggttggc agttcgaacc tccctccgat
     2521 ttagatttcg acacttttgc gcgtacgcat cgtgtcgtca tcgaattcaa gaccgaagtg
     2581 cccgctgggg ccaaggtctt ggttagggat ttgtacgtag tggtaagtga cttaccacga
     2641 gtgcaaattc cgaccgatgt cttgctggtc gatgaagacc tgcttgagat ctagagtgag
     2701 ataagcacac tcgaatgtct ccgaatggaa agttcgtacc accgatagtg gatattgcga
     2761 aatagatttc tgaaagtcgc ttcccggctt tcatgcttgg aaatcttacc tgcgttagca
     2821 gatgcccaca acgtgaagtt gtggatgccc cgttagggaa gcggatccga taccctgtca
     2881 ccggatgtgt tttccggtct gatgagtccg tgaggacgaa acaggactgt cctgcaggag
     2941 ctcgaattcg gtacgctgaa atcaccagtc tctctctaca aatctatctc tctctatttt
     3001 ctccataaat aatgtgtgag tagtttcccg ataagggaaa ttagggttct tatagggttt
     3061 cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc
     3121 tatcaataaa atttctaatt cctaaaacca aaatccagta ctaaaatcca gatcagcttg
     3181 gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     3241 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     3301 cgcccttccc aacagttgcg cagcctgaat ggcgaatgct agagcagctt gagcttggat
     3361 cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg
     3421 gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa
     3481 ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa
     3541 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
     3601 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
     3661 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
     3721 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
     3781 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
     3841 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
     3901 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
     3961 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
     4021 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
     4081 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
     4141 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
     4201 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
     4261 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
     4321 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
     4381 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
     4441 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
     4501 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
     4561 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     4621 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     4681 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     4741 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     4801 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     4861 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     4921 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     4981 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     5041 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     5101 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     5161 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     5221 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     5281 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     5341 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     5401 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     5461 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     5521 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     5581 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     5641 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     5701 aacgggcggt tggccaggcg taagcggctg ggttgcctgc cggccctgca atggcactgg
     5761 aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc
     5821 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
     5881 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
     5941 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
     6001 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
     6061 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
     6121 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
     6181 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
     6241 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
     6301 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
     6361 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
     6421 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
     6481 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
     6541 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
     6601 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
     6661 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc
     6721 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
     6781 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
     6841 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
     6901 aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
     6961 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
     7021 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
     7081 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
     7141 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
     7201 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
     7261 ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc
     7321 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     7381 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     7441 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     7501 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     7561 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     7621 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7681 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7741 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7801 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7861 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7921 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7981 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     8041 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     8101 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     8161 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     8221 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8281 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8341 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8401 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcattctagg
     8461 tactaaaaca attcatccag taaaatataa tattttattt tctcccaatc aggcttgatc
     8521 cccagtaagt caaaaaatag ctcgacatac tgttcttccc cgatatcctc cctgatcgac
     8581 cggacgcaga aggcaatgtc ataccacttg tccgccctgc cgcttctccc aagatcaata
     8641 aagccactta ctttgccatc tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa
     8701 aagacaagtt cctcttcggg cttttccgtc tttaaaaaat catacagctc gcgcggatct
     8761 ttaaatggag tgtcttcttc ccagttttcg caatccacat cggccagatc gttattcagt
     8821 aagtaatcca attcggctaa gcggctgtct aagctattcg tatagggaca atccgatatg
     8881 tcgatggagt gaaagagcct gatgcactcc gcatacagct cgataatctt ttcagggctt
     8941 tgttcatctt catactcttc cgagcaaagg acgccatcgg cctcactcat gagcagattg
     9001 ctccagccat catgccgttc aaagtgcagg acctttggaa caggcagctt tccttccagc
     9061 catagcatca tgtccttttc ccgttccaca tcataggtgg tccctttata ccggctgtcc
     9121 gtcattttta aatataggtt ttcattttct cccaccagct tatatacctt agcaggagac
     9181 attccttccg tatcttttac gcagcggtat ttttcgatca gttttttcaa ttccggtgat
     9241 attctcattt tagccattta ttatttcctt cctcttttct acagtattta aagatacccc
     9301 aagaagctaa ttataacaag acgaactcca attcactgtt ccttgcattc taaaacctta
     9361 aataccagaa aacagctttt tcaaagttgt tttcaaagtt ggcgtataac atagtatcga
     9421 cggagccgat tttgaaaccg cggtgatcac aggcagcaac gctctgtcat cgttacaatc
     9481 aacatgctac cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct
     9541 tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac
     9601 gccgtcccgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc
     9661 ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag
     9721 acaacttaat aacacattgc ggacgttttt aatgtactga attaacgccg aattaattcg
     9781 ggggatctgg attttagtac tggattttgg ttttaggaat tagaaatttt attgatagaa
     9841 gtattttaca aatacaaata catactaagg gtttcttata tgctcaacac atgagcgaaa
     9901 ccctatagga accctaattc ccttatctgg gaactactca cacattatta tggagaaact
     9961 cgagcttgtc gatcgacaga tcccggtcgg catctactct atttctttgc cctcggacga
    10021 gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc ggtccagacg
    10081 gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt
    10141 gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac caagctctga
    10201 tagagttggt caagaccaat gcggagcata tacgcccgga gtcgtggcga tcctgcaagc
    10261 tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc
    10321 cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc gctccagtca
    10381 atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa atccgcgtgc
    10441 acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc gagagcctgc
    10501 gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac atggggatca
    10561 gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg cggtccgaat
    10621 gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat agcctccgcg
    10681 accggttgta gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt
    10741 gaacggcggg agatgcaata ggtcaggctc tcgctaaact ccccaatgtc aagcacttcc
    10801 ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt gtagaaacca
    10861 tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa gctgaaagca
    10921 cgagattctt cgccctccga gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc
    10981 agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcatatctca ttgccccccg
    11041 gatctgcgaa agctcgagag agatagattt gtagagagag actggtgatt tcagcgtgtc
    11101 ctctccaaat gaaatgaact tccttatata gaggaagggt cttgcgaagg atagtgggat
    11161 tgtgcgtcat cccttacgtc agtggagata tcacatcaat ccacttgctt tgaagacgtg
    11221 gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca
    11281 ctgtcggcag aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag
    11341 gtgccacctt ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat
    11401 ccgaggaggt ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct
    11461 gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttcaca
    11521 tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt
    11581 gggtgggggt ccatctttgg gaccactgtc ggcag
//
