LOCUS       sequence-170078-        2007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bioluminescent and ratiometric voltage indicator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Inagaki S, Tsutsui H, Suzuki K, Agetsuma M, Arai Y, Jinno Y, Bai G, 
            Daniels MJ, Okamura Y, Matsuda T, Nagai T
  TITLE     Genetically encoded bioluminescent voltage indicator for 
            multi-purpose use in wide range of bioimaging.
  JOURNAL   Sci Rep. 2017 Feb 13;7:42398. doi: 10.1038/srep42398.
  PUBMED    28205521
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-02-13"; volume: "7:42398. doi"; pages: " 
            10.1038/srep42398"
COMMENT     Sequence Label: LOTUS-V/pcDNA3
FEATURES             Location/Qualifiers
     source          1..2007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             316..828
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             join(1288..1290,1291..1293,1294..2007)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1333..1354)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1594..1613)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1941..1962
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggagggat tcgacggttc agattttagt cctccagctg atttagttgg cgttgacggt
       61 gcagtcatgc ggaacgtcgt tgacgtcacg ataaatggtg acgtcactgc tccgccgaaa
      121 gctgcgccaa gaaaaagtga atcggtaaag aaagttcatt ggaatgatgt agaccaagga
      181 ccgagtgaaa aaccagagac aagacaggag gaacgaatag atatacccga gatttcaggt
      241 ctatggtggg gcgagaatga acatggagtg gacgatggga gaatggagat acctactact
      301 ggtgtagggg atcccatggt cttcacactc gaagatttcg ttggggactg gcgacagaca
      361 gccggctaca acctggacca agtccttgaa cagggaggtg tgtccagttt gtttcagaat
      421 ctcggggtgt ccgtaactcc gatccaaagg attgtcctga gcggtgaaaa tgggctgaag
      481 atcgacatcc atgtcatcat cccgtatgaa ggtctgagcg gcgaccaaat gggccagatc
      541 gaaaaaattt ttaaggtggt gtaccctgtg gatgatcatc actttaaggt gatcctgcac
      601 tatggcacac tggtaatcga cggggttacg ccgaacatga tcgactattt cggacggccg
      661 tatgaaggca tcgccgtgtt cgacggcaaa aagatcactg taacagggac cctgtggaac
      721 ggcaacaaaa ttatcgacga gcgcctgatc aaccccgacg gctccctgct gttccgagta
      781 accatcaacg gagtgaccgg ctggcggctg tgcgaacgca ttctggcgga attcggcgtg
      841 ggccgcgtcc agtttcgtgt ccgagcagtg attgatcatc tagggatgcg agtctttgga
      901 gtcttcctaa ttttcttgga catcatcctc atgatcattg atctcagtct tccaggaaaa
      961 agtgaatctt cacaatcctt ttatgacggg atggctttgg ctctttcttg ttatttcatg
     1021 ctggatttag gattaaggat atttgcctac gggcccaaga atttcttcac caacccctgg
     1081 gaggttgctg atggtttgat tatcgttgtc acattcgtcg tcacgatatt ttacactgtg
     1141 ttagatgaat acgtgcaaga aacaggagcc gatggtttgg ggcagttggt tgtgttggcc
     1201 cgtttgctgc gtgtggttcg attagcaaga atattttatt cccatcaaca aatgaaggct
     1261 tcaagcagaa gaacaatatc actcgagatg gtgagcaagg gcgaggagct gttcaccggg
     1321 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     1381 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc
     1441 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc
     1501 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     1561 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1621 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1681 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     1741 tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
     1801 atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     1861 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac
     1921 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     1981 ctcggcatgg acgagctgta caagtaa
//
