LOCUS       sequence-170080-        8230 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lenti reporter plasmid with M-AAT-specific gRNA target-sequence, to 
            be used with tGFP #26864.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8230)
  AUTHORS   Eggenschwiler R, Moslem M, Fraguas MS, Galla M, Papp O, Naujock M, 
            Fonfara I, Gensch I, Wahner A, Beh-Pajooh A, Mussolino C, Tauscher 
            M, Steinemann D, Wegner F, Petri S, Schambach A, Charpentier E, 
            Cathomen T, Cantz T
  TITLE     Improved bi-allelic modification of a transcriptionally silent locus
            in patient-derived iPSC by Cas9 nickase.
  JOURNAL   Sci Rep. 2016 Dec 2;6:38198. doi: 10.1038/srep38198.
  PUBMED    27910942
REFERENCE   2  (bases 1 to 8230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-12-2"; volume: "6:38198. doi"; pages: " 
            10.1038/srep38198"
COMMENT     Sequence Label: Lenti CGIP + M-AAT target
FEATURES             Location/Qualifiers
     source          1..8230
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      36..45
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(87..108)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(348..367)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     757..778
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(863..1437,1438..1440,1441..1449)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1030..1047)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1257..1276
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             1450..2049
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1450..1469)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1946..1966
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(2105..2121)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2106..2122)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    2159..2747
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2212..2232)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2630..2641)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2834..3067
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3139..3260
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3176..3195)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3230..3249
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3279..3414
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3446..3465)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3447..3465)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3475..3492)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3475..3491)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3484..3506)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3636..4091
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3723..3742)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3933..3954
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4120..4224
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4225..4293,4294..5085)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4443..4462)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5256..5844
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5745..5764
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5998..6015
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6132..6153
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6168..6173,6174..6191,6192..6198)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6206..6222
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6211..6233
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6230..6246
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6230..6246
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6265..6285
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6267..6285
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             6541..6721
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6765..6890
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7383..7616
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7801..7845
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8112..8229
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cattttggca aagaattcct cgagatccac cggtcgccac catggtgagc aagggcgagg
       61 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      121 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      181 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
      241 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      301 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
      361 acaagaccta accaggccgt gcataaggct gtgctgacca tcgacgagaa agggactgaa
      421 gctgctgggg cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
      481 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
      541 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
      601 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
      661 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
      721 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
      781 cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc gcgtcgacgg
      841 gcccgcggaa ttccgccccc cccccctctc cctccccccc ccctaacgtt actggccgaa
      901 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
      961 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     1021 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     1081 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     1141 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
     1201 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     1261 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     1321 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
     1381 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg
     1441 gccacaacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     1501 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     1561 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     1621 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     1681 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     1741 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     1801 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     1861 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     1921 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     1981 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     2041 ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat
     2101 gcatcgatac cgtcgacctc gagggggggc ccggtacaag taaagcggcc gcgtcgacaa
     2161 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     2221 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     2281 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     2341 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     2401 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     2461 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     2521 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     2581 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     2641 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     2701 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattcgagc
     2761 tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     2821 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     2881 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     2941 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     3001 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     3061 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     3121 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     3181 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     3241 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     3301 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     3361 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     3421 tttgcgtcga gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     3481 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     3541 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     3601 ccaacagttg cgcagcctga atggcgaatg gcgcgacgcg ccctgtagcg gcgcattaag
     3661 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     3721 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     3781 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     3841 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     3901 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     3961 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4021 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     4081 gtttacaatt tcccaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4141 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     4201 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     4261 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     4321 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     4381 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     4441 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     4501 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     4561 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     4621 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     4681 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     4741 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     4801 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     4861 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     4921 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     4981 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     5041 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     5101 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5161 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5221 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5281 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5341 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5401 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     5461 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     5521 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     5581 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     5641 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     5701 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     5761 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     5821 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     5881 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     5941 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     6001 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     6061 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     6121 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     6181 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     6241 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
     6301 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
     6361 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
     6421 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
     6481 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacaataaac
     6541 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6601 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6661 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6721 agtggcgccc gaacagggac ctgaaagcga aagggaaacc agagctctct cgacgcagga
     6781 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     6841 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     6901 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     6961 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     7021 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     7081 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     7141 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     7201 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     7261 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     7321 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     7381 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcctca
     7441 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     7501 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     7561 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     7621 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     7681 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     7741 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     7801 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     7861 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     7921 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     7981 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     8041 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     8101 tatcggttaa cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     8161 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     8221 caaaatttta
//
